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echochild
The Padawan Learner

Registered: 08/26/22
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Glass Agar Dishes info needed
#28022266 - 10/28/22 11:59 PM (2 years, 2 months ago) |
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So, I bought some glass (autoclavable) petri dishes off amazon. I wanted agar dishes I could reuse that I could sterilize in my PC.
What I got looks way off. The lids and bottoms don't match. There is a 2-3 mm gap difference when you slide the bottoms all the way to a side. Is that normal for glass dishes?

Also... I have a vayepro hot plate. I have a glass stove and the manufacturer stated they did not recommend using a PC on it. The hot plate, turns on and off to maintain the desired heat setting, making pressure in the PC kind of irregular.
Anyone have any recommendations for a better one that would work with a 23 QT Presto?
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HappyHigh
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Re: Glass Agar Dishes info needed [Re: echochild]
#28022269 - 10/29/22 12:07 AM (2 years, 2 months ago) |
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Mine are the same, I was sure to use tape around the whole edge of dish to keep it sturdy. I used in PC worked fine.
-------------------- Had I the heavens' embroidered cloths,
Enwrought with golden and silver light,
The blue and the dim and the dark cloths
Of night and light and the half light,
I would spread the cloths under your feet:
But I, being poor, have only my dreams;
I have spread my dreams under your feet;
Tread softly because you tread on my dreams.
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echochild
The Padawan Learner

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Re: Glass Agar Dishes info needed [Re: HappyHigh]
#28022272 - 10/29/22 12:10 AM (2 years, 2 months ago) |
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How are your contam rates with them? That seems like an awfully big gap...
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HappyHigh
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Re: Glass Agar Dishes info needed [Re: echochild] 2
#28022287 - 10/29/22 12:26 AM (2 years, 2 months ago) |
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They have "parafilm" what most ppl use but it is a lil $$. I just use .3 micron tape 4$, round the outer edge then fold under too seal, stack fine. During PC I fold them in foil and leave it sealed till I use it.
-------------------- Had I the heavens' embroidered cloths,
Enwrought with golden and silver light,
The blue and the dim and the dark cloths
Of night and light and the half light,
I would spread the cloths under your feet:
But I, being poor, have only my dreams;
I have spread my dreams under your feet;
Tread softly because you tread on my dreams.
Edited by HappyHigh (10/29/22 12:27 AM)
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echochild
The Padawan Learner

Registered: 08/26/22
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Re: Glass Agar Dishes info needed [Re: HappyHigh]
#28022289 - 10/29/22 12:35 AM (2 years, 2 months ago) |
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Yeah, I have parafilm. I tried micropore tape previously, but noticed I was having an unusually high rate of contam on those prepoured plates compared to the ones I was using parafilm with. Maybe I was just messing up my sterile technique, but as soon as I went back to parafilm, I stopped having the issue.
Thing is, the bottoms of the plates, and the bottom edges of the tops are pretty much flush with one another. I'm not sure that parafilm would grip that well, unless I'm doing several wraps with it (if even that would work?).
Maybe I will need to do another test with the micropore tape...
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HappyHigh
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Re: Glass Agar Dishes info needed [Re: echochild]
#28022299 - 10/29/22 12:53 AM (2 years, 2 months ago) |
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Yeah I wondered the same about the parafilm if it would hold.
-------------------- Had I the heavens' embroidered cloths,
Enwrought with golden and silver light,
The blue and the dim and the dark cloths
Of night and light and the half light,
I would spread the cloths under your feet:
But I, being poor, have only my dreams;
I have spread my dreams under your feet;
Tread softly because you tread on my dreams.
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echochild
The Padawan Learner

Registered: 08/26/22
Posts: 226
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Re: Glass Agar Dishes info needed [Re: HappyHigh]
#28022301 - 10/29/22 12:59 AM (2 years, 2 months ago) |
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My crystal ball is showing me I have experimentation in my future
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Toasted
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Re: Glass Agar Dishes info needed [Re: echochild]
#28022305 - 10/29/22 01:09 AM (2 years, 2 months ago) |
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The gaps are fine but more expensive petris do have less of a gap
Make sure edges are dry so your parafilm or saran wrap doesn't slip off
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echochild
The Padawan Learner

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Re: Glass Agar Dishes info needed [Re: Toasted]
#28022306 - 10/29/22 01:13 AM (2 years, 2 months ago) |
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Quote:
Toasted said: The gaps are fine but more expensive petris do have less of a gap
Make sure edges are dry so your parafilm or saran wrap doesn't slip off
Saran wrap.
Man... I need to go to bed. I've been PC'ing PF Tek pucks and was having issues with my circuit breaker deciding to be an asshole every time I would get close to getting ready to pressurize.
Saran wrap would indeed do the job and not obstruct my view of the plates. Now I just need to give them all a thorough look over. I noticed a crack in one of them.
Thank you all
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bongoman
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Re: Glass Agar Dishes info needed [Re: echochild] 1
#28026490 - 10/31/22 04:37 PM (2 years, 2 months ago) |
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I'v been using grafting tape with my glass petris.
But I notice that the extra weight of the glass material tends to put more pressure on the wrap when you are handling the plates and the wrap potentially can work itself loose.
I wonder if lab parafilm holds better?
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Shrimps
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Registered: 10/13/22
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Re: Glass Agar Dishes info needed [Re: bongoman]
#28026497 - 10/31/22 04:38 PM (2 years, 2 months ago) |
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Parafilm is great, but expensive compared to saran. If money didn't matter, I would go with parafilm ^^
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kirkeng
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Re: Glass Agar Dishes info needed [Re: Shrimps] 1
#28026956 - 10/31/22 08:54 PM (2 years, 2 months ago) |
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If you don’t want to do pre-pours with glass plates I recommend sterilizing clean dishes in the oven 1 hour at 400F does the trick, stacks wrapped in foil. Make sure they are clean and devoid of debris or sticky stuff if you use tape. Pouring glass dishes can also be cumbersome I pour in stacks of 5 because of this. Clean your dishes sooner rather than later when your done with them. If you wait to long you may need to scrub, if cleaned in good time you can just rinse them with water and dry them before sterilizing again. If you live by a university it’s worthwhile to contact them if they have a reuse program, I’ve gotten hundreds of old plates at .25-.5 cents a piece, when they out with the old and in with the new.
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Suckatshrooms
Seriously, don’t listen to me.


Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
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Re: Glass Agar Dishes info needed [Re: echochild]
#28026971 - 10/31/22 09:01 PM (2 years, 2 months ago) |
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Quote:
echochild said: So, I bought some glass (autoclavable) petri dishes off amazon. I wanted agar dishes I could reuse that I could sterilize in my PC.
What I got looks way off. The lids and bottoms don't match. There is a 2-3 mm gap difference when you slide the bottoms all the way to a side. Is that normal for glass dishes?

Also... I have a vayepro hot plate. I have a glass stove and the manufacturer stated they did not recommend using a PC on it. The hot plate, turns on and off to maintain the desired heat setting, making pressure in the PC kind of irregular.
Anyone have any recommendations for a better one that would work with a 23 QT Presto?
Send them back and get Pyrex brand if you’re after quality. They’re perfect. If budget is a concern, I would go the opposite direction and get dirt cheap, non borosilicate glass. You can still pre pour and autoclave, but to be safe, let the PC cool to room temp before you open it.
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Suckatshrooms
Seriously, don’t listen to me.


Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
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Re: Glass Agar Dishes info needed [Re: bongoman]
#28026977 - 10/31/22 09:04 PM (2 years, 2 months ago) |
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Quote:
bongoman said: I'v been using grafting tape with my glass petris.
But I notice that the extra weight of the glass material tends to put more pressure on the wrap when you are handling the plates and the wrap potentially can work itself loose.
I wonder if lab parafilm holds better?
Parafilm doesn’t budge on my glass plates.
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
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Re: Glass Agar Dishes info needed [Re: echochild] 2
#28027014 - 10/31/22 09:24 PM (2 years, 2 months ago) |
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Quote:
echochild said: How are your contam rates with them? That seems like an awfully big gap...
That's a terrible answer. Wrap the dish (like every petri dish, plastic or glass) with cling wrap or grafting tape and that gap now has 0 impact.
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!.
We don't own things - things own us.
Semi-solid liquid culture (SSLC)
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echochild
The Padawan Learner

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Re: Glass Agar Dishes info needed [Re: Rusty2096]
#28027097 - 10/31/22 10:22 PM (2 years, 2 months ago) |
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Quote:
Rusty2096 said:
Quote:
echochild said: How are your contam rates with them? That seems like an awfully big gap...
That's a terrible answer. Wrap the dish (like every petri dish, plastic or glass) with cling wrap or grafting tape and that gap now has 0 impact.
Terrible question you mean? That was a question to a response.
Well, I PC'ed them yesterday and poured some agar. They are a bit cumbersome to work with. Use cling wrap to seal them, and they are in the waiting phase for a week to make sure no accidental contamination happened.
Before I PC'ed them I tested with parafilm. Considering the edges of the top are flush with the bottom, parafilm didn't play well, or I couldn't figure out how to make it play well like my other dishes, so I went with the wrap.
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hazyhorse
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Re: Glass Agar Dishes info needed [Re: echochild] 1
#28027133 - 10/31/22 10:54 PM (2 years, 2 months ago) |
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hi! i use those glass dishes, the gap on the lid is totally normal. seems huge but is just how those cheaper glass dishes are. just use some cling wrap/seran wrap (same thing i just don’t know what everyone else called it). i cut my rolls about 1”-2” wide. you definitely want to make it wide enough to completely seal the dishes. i think parafilm would be too thin for my glass petri dishes, but i’ve never used parafilm so idk. i have a write up in my sig about using them for no pour too, if you’re sterilizing the plates in the PC it’s nicer to just no pour them imo but like a previous comment said they can just be oven sterilized if you’re trying to pour them. sandman420 has a good write up on that but it’s pretty straight forward
-------------------- you're not the first to set foot here, just another
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TheUsualSuspect
never tell me the odds


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Re: Glass Agar Dishes info needed [Re: hazyhorse]
#28027159 - 10/31/22 11:29 PM (2 years, 2 months ago) |
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Out of curiosity, why the preference for glass pitris?
I use plastic ones pre sterilized. I like that they fit together tight and also the gap is in the center of the two so parafilm works fine (although I hate parafilm and am never convinced it 'stuck' where it was put). I also like that if I look in and its all nasty, I just chuck it in the trash without opening to expose myself to whatever is in there.
There must be benefits to glass, or is it just the plastic waste?
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DERRAYLD
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Plastic waste, clarity and infinitely reusable, I also use pp cups but they too are not disposable. Disposable plates are incredibly wasteful and don't force you to focus in anyway.
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echochild
The Padawan Learner

Registered: 08/26/22
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Re: Glass Agar Dishes info needed [Re: DERRAYLD]
#28027211 - 11/01/22 12:47 AM (2 years, 2 months ago) |
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Quote:
DERRAYLD said: Plastic waste, clarity and infinitely reusable, I also use pp cups but they too are not disposable. Disposable plates are incredibly wasteful and don't force you to focus in anyway.
Derrayld is on the nose. I'm not the most eco friendly person ever, but if I can, I do. I liked using ketchup cups, but hate how small they are and that they can't be reused.
Also... combined with making my own agar, a lot of money savings in the long run.
Hazy, thanks for giving me ONE MORE TAB to read... LOL joking. Thanks for the info, I will give it a read when I have more brains tomorrow.
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