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OfflineFunfarm
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Agar time cycle
    #28018184 - 10/26/22 07:02 PM (2 years, 2 months ago)

Not at the point in the hobby of doing my own agar production, so Grandma was kind enough to buy me a number of poured plates from a vendor online. I have a fruit that I would like to clone, I was going to take specimen collections from it and place it on 5 separate plates. Is it ok to store the unused plates in the refrigerator for later use until the 5 plates I attempt to get mycelium with are ready to be transferred?

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Invisiblebigfootscreepyuncl
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Re: Agar time cycle [Re: Funfarm] * 1
    #28018198 - 10/26/22 07:10 PM (2 years, 2 months ago)

Unused and properly wrapped you can store agar plates at room temp for a long time. I just germinated spores on 11 month old plates. If you have an inoculated plate either by spore or clone material you can allow it to colonize the plate and put it in the fridge. I would recommend putting the plates in ziplock bags. They can be kept in the fridge for about 6 months before you should start thinking about transferring them although I would recommend refrigerating an already cleaned up plate.

Maybe consider putting a tissue sample to one plate and using the remaining 4 for transfers.


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OfflineFunfarm
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Re: Agar time cycle [Re: bigfootscreepyuncl]
    #28018235 - 10/26/22 07:25 PM (2 years, 2 months ago)

Sure thing, I'll take one fruit sample on one plate, wait for growth and transfer to the other plates. It sounds like both used and unused plates can go in the fridge?

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OfflineRusty2096
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Re: Agar time cycle [Re: Funfarm]
    #28018282 - 10/26/22 07:48 PM (2 years, 2 months ago)

None of your plates need a fridge. You are starting out. You will have used em all way before they dry out (or pin even)


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Invisiblebigfootscreepyuncl
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Re: Agar time cycle [Re: Rusty2096] * 1
    #28018521 - 10/26/22 11:04 PM (2 years, 2 months ago)

Rusty brought up a good point - I would honestly put clone tissue to 1 or 2 plates and let them sit at room temp to see if they throw off a plate pin or two. If they do, just pull the pin off the plate, transfer it to a new plate and use that for your next batch :thumbup:


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OfflineTheUsualSuspect
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Re: Agar time cycle [Re: bigfootscreepyuncl]
    #28018596 - 10/27/22 01:30 AM (2 years, 2 months ago)

I would recommend if they arent sealed by the vendor, that you put the ones you dont use in a sealed zip-loc bag so they dont dry out and to reduce chance they contam before using. Maybe not needed but I like redundancy.

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OfflineFunfarm
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Re: Agar time cycle [Re: TheUsualSuspect]
    #28019866 - 10/27/22 05:26 PM (2 years, 2 months ago)

Thinking ziplock and fridge. Thanks to all for the direction, excited to get a healthy culture and sector in hopes of cloned fruits canopy style.

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OfflineRusty2096
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Re: Agar time cycle [Re: Funfarm]
    #28019893 - 10/27/22 05:48 PM (2 years, 2 months ago)

You got this all wrong brother. But they, it's your fridge :shrug:

(reducing sectoring, even to the point of getting a true isolate, has nothing to do with canopies. In fact, many iso won't even fruit)


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OfflineFunfarm
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Re: Agar time cycle [Re: Rusty2096]
    #28021612 - 10/28/22 02:57 PM (2 years, 2 months ago)

Rusty what do you suggest? I just want to clone a healthy fruit in hopes of better yield

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Offlinehazyhorse
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Re: Agar time cycle [Re: Funfarm]
    #28021638 - 10/28/22 03:16 PM (2 years, 2 months ago)

just clone the best fruit in the flush by taking some inner tissue, leave it in an agar plate until you can take a few transfers, then grow it out to see how it performs. agar doesn’t need to be refrigerated unless you’re intentionally trying to slow down a culture


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OfflineFunfarm
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Re: Agar time cycle [Re: hazyhorse]
    #28021971 - 10/28/22 07:21 PM (2 years, 2 months ago)

Turn the plates upside down put them waiting in ziplocks and store at room temperature. That's what Grandma wants me to do. Thank you for responding to all

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