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InvisibleTexastransplant
Cosmic Voyager
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Registered: 09/15/22
Posts: 115
Genetic Library
    #28017364 - 10/26/22 10:45 AM (2 years, 2 months ago)

I transferred a number of potential colonies from some plates I nocked up from syringe spores. Most have done well so far, with only a handful showing two colonies growing, or mold (mold on the other side of the dish from the myc). These have had a fresh transfer as of today to regular agar (testing out my ffu, if they are not doing proper later on will go to water to attempt to clean up).

It looks like I will have more success than I was expecting (all plates are roughly half dollar - quarter size). I only plan to use 1 sample at a time to start bulk growing, how long can I expect to be able to store these other plates in my fridge until further use is needed?

7g agar agar and 7g MEA - was the recipe for the nut agar.

I would only need to double wrap them again as well for preparation of going in the fridge?

Should I get a small ice box to put them inside of in the fridge even if it only gets opened by myself for related materials? 1-2 a day?

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Invisiblebakedbeings
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Registered: 09/01/20
Posts: 4,563
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Re: Genetic Library [Re: Texastransplant] * 1
    #28017387 - 10/26/22 10:58 AM (2 years, 2 months ago)

i wrap plates in foil then plastic. seems to work ok. if you want total assurance you can store cultures in distilled water, seems to be the longest lasting


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InvisibleTexastransplant
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Registered: 09/15/22
Posts: 115
Re: Genetic Library [Re: bakedbeings]
    #28017441 - 10/26/22 11:28 AM (2 years, 2 months ago)

Quote:

bakedbeings said:
i wrap plates in foil then plastic. seems to work ok. if you want total assurance you can store cultures in distilled water, seems to be the longest lasting




Ill look into the distilled water as an option, guessing they wont last more than a few months in the fridge?

I have a nut plate with a clone thats gotten far along with no contam, would it be ok to spawn from this sample or should I transfer to a clean plate to ensure no contam from the sample?


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OfflineiceNock


Registered: 11/15/21
Posts: 335
Last seen: 1 year, 4 months
Re: Genetic Library [Re: Texastransplant]
    #28017525 - 10/26/22 12:39 PM (2 years, 2 months ago)

Quote:

Texastransplant said:
Quote:

bakedbeings said:
i wrap plates in foil then plastic. seems to work ok. if you want total assurance you can store cultures in distilled water, seems to be the longest lasting




Ill look into the distilled water as an option, guessing they wont last more than a few months in the fridge?






From what I read they can last years in that situation.

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InvisibleShrimps
Traveler
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Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
Re: Genetic Library [Re: iceNock]
    #28017532 - 10/26/22 12:42 PM (2 years, 2 months ago)

I can recommend: https://www.shroomery.org/forums/showflat.php/Number/27778467/fpart/1/vc/1

Great tek, also read somewhere that with that kind of tek mush cultus could be revived after 20years. (But can't remember the source i read that :frown: )


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InvisibleTexastransplant
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Registered: 09/15/22
Posts: 115
Re: Genetic Library [Re: Shrimps]
    #28017602 - 10/26/22 01:21 PM (2 years, 2 months ago)

Quote:

Shrimps said:
I can recommend: https://www.shroomery.org/forums/showflat.php/Number/27778467/fpart/1/vc/1

Great tek, also read somewhere that with that kind of tek mush cultus could be revived after 20years. (But can't remember the source i read that :frown: )




Yeah I looked into slants, not what I am working toward atm. Planning to grow and assess each individual colony I have going to determine which are good, which to keep, and which to shit can. Once I have that, then I can slant a colony I deem worthy.

Thats why my initial query was for something in the ball park of 6-12m, I know I didnt specify was looking for data on the storage tek.

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Offlineschpat
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Registered: 09/26/21
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Re: Genetic Library [Re: Texastransplant] * 1
    #28018611 - 10/27/22 02:12 AM (2 years, 2 months ago)

I've only stored plates in the fridge for about 6 months, they worked fine and I think they could have gone double that time.  I think it may depend on how deep your agar is, the deeper the longer they will take to dry out.


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