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InvisibleMushroomsee
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Registered: 04/29/18
Posts: 195
Failed spawn bags, help please
    #28015565 - 10/25/22 08:05 AM (2 years, 2 months ago)

Hi. I set up 10 spawn bags. Injected with spores. 6 of the 10 bags looks really wet now after a few days, and smell sour. While the other 4 are drier and smell normal. What has gone wrong?


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OfflineScrewup
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Registered: 01/27/22
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Re: Failed spawn bags, help please [Re: Mushroomsee] * 1
    #28015568 - 10/25/22 08:08 AM (2 years, 2 months ago)

You used syringes on pre made bags.

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InvisibleMushroomsee
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Registered: 04/29/18
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Re: Failed spawn bags, help please [Re: Screwup]
    #28015575 - 10/25/22 08:18 AM (2 years, 2 months ago)

No, I made the bags myself. Boiled the grains, sealed the bag, PC for 90 min at 15 psi. Let the bags cool down for a day. Then injected spores. I have done this many times before and it has always gone well. I don't understand what went wrong now? Was the grains to wet maybe? They did not look to wet but maybe? Or what else could have caused it?

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InvisibleMushroomsee
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Posts: 195
Re: Failed spawn bags, help please [Re: Mushroomsee]
    #28015578 - 10/25/22 08:20 AM (2 years, 2 months ago)

It smells something like beer or sourdough

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OfflineKROM
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Re: Failed spawn bags, help please [Re: Mushroomsee]
    #28015580 - 10/25/22 08:22 AM (2 years, 2 months ago)

How big are those bags? I think I saw P9 pcs 4qt grain bags for 3.5-4 hours. Also, here’s a thread on why spores to grain is not recommended.


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OfflineScrewup
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Re: Failed spawn bags, help please [Re: Mushroomsee]
    #28015582 - 10/25/22 08:23 AM (2 years, 2 months ago)

Quote:

Mushroomsee said:
No, I made the bags myself. Boiled the grains, sealed the bag, PC for 90 min at 15 psi. Let the bags cool down for a day. Then injected spores. I have done this many times before and it has always gone well. I don't understand what went wrong now? Was the grains to wet maybe? They did not look to wet but maybe? Or what else could have caused it?



Not long enough PC time by most people standard for baba, you used syringes which are full of bacteria. They were perhaps too wet but those two top ones are your biggest issues. They’re done for so toss em.

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InvisibleMushroomsee
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Re: Failed spawn bags, help please [Re: KROM]
    #28015586 - 10/25/22 08:27 AM (2 years, 2 months ago)

3 liter bags

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InvisibleMushroomsee
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Registered: 04/29/18
Posts: 195
Re: Failed spawn bags, help please [Re: Screwup]
    #28015590 - 10/25/22 08:29 AM (2 years, 2 months ago)

Quote:

Screwup said:
Quote:

Mushroomsee said:
No, I made the bags myself. Boiled the grains, sealed the bag, PC for 90 min at 15 psi. Let the bags cool down for a day. Then injected spores. I have done this many times before and it has always gone well. I don't understand what went wrong now? Was the grains to wet maybe? They did not look to wet but maybe? Or what else could have caused it?



Not long enough PC time by most people standard for baba, you used syringes which are full of bacteria. They were perhaps too wet but those two top ones are your biggest issues. They’re done for so toss em.




I have always used Spore syringe without problem. And 4 of the 6 bags do good. Something else most be wrong. Maybe to wet grains? Can that be the problem?

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OfflineScrewup
Googles your dumb questions
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Re: Failed spawn bags, help please [Re: Mushroomsee]
    #28015596 - 10/25/22 08:33 AM (2 years, 2 months ago)

That was then and this is now. Grain bags with syringes will give you issues eventually. Your grains being too wet can cause an issue too. PC them longer as well.

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Offlineschpat
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Re: Failed spawn bags, help please [Re: Mushroomsee]
    #28015601 - 10/25/22 08:38 AM (2 years, 2 months ago)

Quote:

Mushroomsee said:
Quote:

Screwup said:
Quote:

Mushroomsee said:
No, I made the bags myself. Boiled the grains, sealed the bag, PC for 90 min at 15 psi. Let the bags cool down for a day. Then injected spores. I have done this many times before and it has always gone well. I don't understand what went wrong now? Was the grains to wet maybe? They did not look to wet but maybe? Or what else could have caused it?



Not long enough PC time by most people standard for baba, you used syringes which are full of bacteria. They were perhaps too wet but those two top ones are your biggest issues. They’re done for so toss em.




I have always used Spore syringe without problem. And 4 of the 6 bags do good. Something else most be wrong. Maybe to wet grains? Can that be the problem?




Dude, despite the question around PC time on large bags, spores are inherently not sterile.  You may have gotten lucky man mnay time so far, but it's almost 100% the spore syringe.


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InvisibleMushroomsee
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Posts: 195
Re: Failed spawn bags, help please [Re: schpat]
    #28015674 - 10/25/22 09:51 AM (2 years, 2 months ago)

But this is 2 days after I set up my bags. If it had been the fault of the spore syringe, it should have taken longer? I have the bags in a closet where it is 28 degrees. It seems that the heat caused the bags to become so wet and smell sour. Surely it must have been because there was too much water in the grains?

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Invisiblebakedbeings
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Re: Failed spawn bags, help please [Re: Mushroomsee] * 1
    #28015678 - 10/25/22 10:03 AM (2 years, 2 months ago)

little of column A, little of column B


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InvisibleMushroomsee
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Re: Failed spawn bags, help please [Re: bakedbeings]
    #28015755 - 10/25/22 11:10 AM (2 years, 2 months ago)

What can I do next time to try to prevent this? I will continue with the spore syringe as I have never had a problem with it.

Should I make my seeds a little drier?

I see in the bag that it's a bit sticky too. You see starch, sort of.

They must have been overcooked? Or?

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OfflineSingularFusion
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Re: Failed spawn bags, help please [Re: Mushroomsee] * 1
    #28015761 - 10/25/22 11:17 AM (2 years, 2 months ago)

it was your spore syringe... seriously

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Invisiblebakedbeings
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Re: Failed spawn bags, help please [Re: Mushroomsee] * 1
    #28015762 - 10/25/22 11:17 AM (2 years, 2 months ago)

if you want consistent results follow trusted teks


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OfflineJaksavage
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Re: Failed spawn bags, help please [Re: Mushroomsee]
    #28015769 - 10/25/22 11:30 AM (2 years, 2 months ago)

28c = 82f= too hot.

Others have pointed at your sterilizing time being too short.

You have heard our thinking on syringes.

Fix the temp and steri time and try again.

:goodluck:


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InvisibleMushroomsee
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Registered: 04/29/18
Posts: 195
Re: Failed spawn bags, help please [Re: Jaksavage]
    #28015826 - 10/25/22 12:06 PM (2 years, 2 months ago)

Quote:

Jaksavage said:
28c = 82f= too hot.

Others have pointed at your sterilizing time being too short.

You have heard our thinking on syringes.

Fix the temp and steri time and try again.

:goodluck:




Thank you brother. I'm boiling new bags now. This time I will let them stand at room temperature of 24 degrees instead of in the cabinet with element where it was 28 degrees. And then I will try to boil them less instead of more. Then PC for 120 min instead of 90 min.

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OfflineJaksavage
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Re: Failed spawn bags, help please [Re: Mushroomsee]
    #28015837 - 10/25/22 12:11 PM (2 years, 2 months ago)

If you pc is packed full, boil for 2.5 to 3 hrs.


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:takingnotes:


The Shroomery is the biggest library with the coolest librarians

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InvisibleMushroomsee
Stranger
Registered: 04/29/18
Posts: 195
Re: Failed spawn bags, help please [Re: Jaksavage]
    #28016198 - 10/25/22 03:50 PM (2 years, 2 months ago)

Quote:

Jaksavage said:
If you pc is packed full, boil for 2.5 to 3 hrs.




Thanks for the help brother

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