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OfflineSirPsycho
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins] * 3
    #28014830 - 10/24/22 07:37 PM (1 year, 3 months ago)

One word: tweezers


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineRotnpins
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: SirPsycho] * 2
    #28014839 - 10/24/22 07:41 PM (1 year, 3 months ago)

Quote:

SirPsycho said:
One word: tweezers




Yeah.. I've taken tissue samples from plate pins with tweezers because the plate was sus... and I'll be doing the same with semps, but I figure it would be really easy to just cut a piece of a stipe as well, toss it on a WA plate and give it a try


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OfflineSirPsycho
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins] * 2
    #28014847 - 10/24/22 07:42 PM (1 year, 3 months ago)

Quote:

Rotnpins said:
Quote:

SirPsycho said:
One word: tweezers




Yeah.. I've taken tissue samples from plate pins with tweezers because the plate was sus... and I'll be doing the same with semps, but I figure it would be really easy to just cut a piece of a stipe as well, toss it on a WA plate and give it a try



As long as you keep track, I'll keep watching :super:


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineCJD
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: SirPsycho]
    #28014848 - 10/24/22 07:43 PM (1 year, 3 months ago)

Quote:

SirPsycho said:
Quote:

CJD said:
What makes it hard?  Just the fruits are so small or they don't take for some reason?



Just being so small. I had 2 clean plates out of 3 and the third contamed on the side not on the flesh sample. Just a bitch and a half to handle.




OK that is what I figured.  I struggled with my first few clones for the same reason.  None of the fruits were "solid stipes" & my razor I use isn't as sharp as it should be.  Might need to try tweezers like you mentioned to Rotn.


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OfflineRotnpins
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: SirPsycho]
    #28014852 - 10/24/22 07:45 PM (1 year, 3 months ago)

Quote:

SirPsycho said:
Quote:

Rotnpins said:
Quote:

SirPsycho said:
One word: tweezers




Yeah.. I've taken tissue samples from plate pins with tweezers because the plate was sus... and I'll be doing the same with semps, but I figure it would be really easy to just cut a piece of a stipe as well, toss it on a WA plate and give it a try



As long as you keep track, I'll keep watching :super:




For sure.. I'd mostly be doing it to share the results in the WA thread.  I need to finish and post some various WA projects, but I've been lagging a bit the past couple weeks.


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OfflineSirPsycho
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins] * 1
    #28014857 - 10/24/22 07:48 PM (1 year, 3 months ago)

Quote:

Rotnpins said:
Quote:

SirPsycho said:
Quote:

Rotnpins said:
Quote:

SirPsycho said:
One word: tweezers




Yeah.. I've taken tissue samples from plate pins with tweezers because the plate was sus... and I'll be doing the same with semps, but I figure it would be really easy to just cut a piece of a stipe as well, toss it on a WA plate and give it a try



As long as you keep track, I'll keep watching :super:




For sure.. I'd mostly be doing it to share the results in the WA thread.  I need to finish and post some various WA projects, but I've been lagging a bit the past couple weeks.



That pops up in my feed, don't worry :wink:


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineLand TroutM
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: SirPsycho] * 6
    #28014910 - 10/24/22 08:16 PM (1 year, 3 months ago)

I love these #12s


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Invisiblenosf3r4tu

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Posts: 775
Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins] * 1
    #28015263 - 10/24/22 11:43 PM (1 year, 3 months ago)

Quote:

Rotnpins said:
Quote:

SirPsycho said:
Quote:

CJD said:
What makes it hard?  Just the fruits are so small or they don't take for some reason?



Just being so small. I had 2 clean plates out of 3 and the third contamed on the side not on the flesh sample. Just a bitch and a half to handle.




I'm thinking once my fruits mature (so not any time in the far future) I'm going to just cut a piece of stipe (not inner tissue) and toss it on a WA plate.. might be an easy way to clone them ๐Ÿค”



Dip it in 3% H2O2 for 5/7 minutes before tossing it on the plate. That's how I used to clone pans and it always worked.


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OfflineSirPsycho
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: nosf3r4tu] * 11
    #28016159 - 10/25/22 03:24 PM (1 year, 3 months ago)



Not quite there yet, maybe another week.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Offlinesmalltalk_canceled
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: SirPsycho] * 2
    #28016199 - 10/25/22 03:53 PM (1 year, 3 months ago)

maybe one should just look for fast pinners on agar with this if one is considering the culture work?


--------------------
Willpower is the one true virtue



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OfflineSirPsycho
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: smalltalk_canceled] * 1
    #28016205 - 10/25/22 03:55 PM (1 year, 3 months ago)

I personally have had horrible luck with cloning plate pins and stones but others swear by it so ya know.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineRotnpins
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: SirPsycho]
    #28016210 - 10/25/22 03:57 PM (1 year, 3 months ago)

Quote:

SirPsycho said:


Not quite there yet, maybe another week.




:awesomenod:

:ancientaliens:
Please tell me that this is a clone..


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OfflineSirPsycho
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins] * 2
    #28016228 - 10/25/22 04:05 PM (1 year, 3 months ago)

Quote:

Rotnpins said:
Quote:

SirPsycho said:


Not quite there yet, maybe another week.




:awesomenod:

:ancientaliens:
Please tell me that this is a clone..



It is, I cloned a fatty from my first run. In retrospect, I probably shouldn't have cloned a fruit that was bent at a 90ยฐ angle... didn't expect bendiness/sensitivety to light source(:shrug:) to be a trait that carried over


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineRotnpins
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: SirPsycho] * 1
    #28016237 - 10/25/22 04:08 PM (1 year, 3 months ago)

Quote:

SirPsycho said:
Quote:

Rotnpins said:
Quote:

SirPsycho said:


Not quite there yet, maybe another week.




:awesomenod:

:ancientaliens:
Please tell me that this is a clone..



It is, I cloned a fatty from my first run. In retrospect, I probably shouldn't have cloned a fruit that was bent at a 90ยฐ angle... didn't expect bendiness/sensitivety to light source(:shrug:) to be a trait that carried over




Interesting... I just cloned a Nat fruit from a cluster that was all bent over.. wonder if I'll notice something similar


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OfflineSirPsycho
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins]
    #28016249 - 10/25/22 04:11 PM (1 year, 3 months ago)

Quote:

Rotnpins said:
Quote:

SirPsycho said:
Quote:

Rotnpins said:
Quote:

SirPsycho said:


Not quite there yet, maybe another week.




:awesomenod:

:ancientaliens:
Please tell me that this is a clone..



It is, I cloned a fatty from my first run. In retrospect, I probably shouldn't have cloned a fruit that was bent at a 90ยฐ angle... didn't expect bendiness/sensitivety to light source(:shrug:) to be a trait that carried over




Interesting... I just cloned a Nat fruit from a cluster that was all bent over.. wonder if I'll notice something similar



:popcorn:


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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InvisibleEgon_Spengler
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: SirPsycho] * 1
    #28016333 - 10/25/22 04:49 PM (1 year, 3 months ago)

Quote:

SirPsycho said:


Not quite there yet, maybe another week.




:awesomenod:


--------------------

   


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Invisiblekirkeng
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Egon_Spengler] * 11
    #28016348 - 10/25/22 05:00 PM (1 year, 3 months ago)

Well looks like Iโ€™m down to my last tub of four. Woke up today and the stalled tub had premonitions of mold as well as the mediocre tub. At least the one that filled in the best is holding out. I suspect the sparse pinning of the others was a condition of the latent infection, gives me hope this final one will make it the distance. Caps are beginning to open up and the section in the back is catching up to the maturity of the rest. For some reason I expected them to be a bit bigger, like tampanensis size, I assume theyโ€™re still a ways out from harvest so guess Iโ€™ll see.


--------------------


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OfflineRotnpins
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: nosf3r4tu] * 8
    #28016427 - 10/25/22 05:44 PM (1 year, 3 months ago)



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OfflineAwestruck
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: Rotnpins]
    #28016597 - 10/25/22 07:02 PM (1 year, 3 months ago)

Oh hello, little pins!


--------------------
Repaying my karmic debt to the Shroomery.
Looking for a Pan cambo print.


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OfflineHindsight
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Re: Official Ps. Subtropicales/Semperviva Thread [Re: kirkeng]
    #28016760 - 10/25/22 08:58 PM (1 year, 3 months ago)

Quote:

kirkeng said:
Well looks like Iโ€™m down to my last tub of four. Woke up today and the stalled tub had premonitions of mold as well as the mediocre tub. At least the one that filled in the best is holding out. I suspect the sparse pinning of the others was a condition of the latent infection, gives me hope this final one will make it the distance. Caps are beginning to open up and the section in the back is catching up to the maturity of the rest. For some reason I expected them to be a bit bigger, like tampanensis size, I assume theyโ€™re still a ways out from harvest so guess Iโ€™ll see.





Thatโ€™s going to be stellar!


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