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OfflineEnkidu
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PE f-line
    #28013189 - 10/23/22 08:07 PM (2 years, 2 months ago)

Is there anything I need to know for a grow using PE f-line ?

I've never grown PE before, only ever cubensis

I have PE f-line on agar as well as PE


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InvisiblefahtsterM
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Re: PE f-line [Re: Enkidu] * 1
    #28013285 - 10/23/22 09:12 PM (2 years, 2 months ago)

Got pics of the f-line?  Never heard of it. 

Thick 2” compressed top layer for regular PE for sure.  That’s here

You can also case with peat/verm but that also needs pasteurizing.  ^^^the pseudo layer works well and just uses more CV.  PE is a cubensis btw

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OfflineEnkidu
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Re: PE f-line [Re: fahtster]
    #28013320 - 10/23/22 09:36 PM (2 years, 2 months ago)

Right, guess I meant I've never worked with any PE varieties and only regular cebes

I knew some PE called for the casing but I thought some you could just run with a top layer at spawn

Appreciate the link, will go that route and take your advice

No pics, idk much about PE varieties, someone sent me swabs or prints I don't even remember now, but that was what the label said


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OfflineLogicaL ChaosM
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Re: PE f-line [Re: Enkidu]
    #28013477 - 10/24/22 01:02 AM (2 years, 2 months ago)

The added top layer on your substrate is a casing: https://www.shroomery.org/13871/Casing

Never heard of "F line" but it sounds like a name given to track cloning/cultures by the original grower perhaps :strokebeard:

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Re: PE f-line [Re: LogicaL Chaos] * 2
    #28013520 - 10/24/22 02:05 AM (2 years, 2 months ago)

:whathesaid:
My guess is that if no pics exist, or history differentiating this culture from any other PE out there, then the source you received your spores from had likely germinated a plate, taken 6 (or more) transfers from germination, labeled them A-F (and so on) to keep them separate in their records while growing out and fruiting multiple cultures in order to chase down a “line” worth cloning/printing/swabbing.


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OfflineSexBurrito
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Re: PE f-line [Re: Egon_Spengler]
    #28013579 - 10/24/22 04:50 AM (2 years, 2 months ago)

The pseudo case doesn't work well for PE in my experience. I did that to 8 PE sb's and they all blobed out.


Just cased these sweater boxes after full colonization with cvg and no more blobs. 



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OfflineDERRAYLD
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Re: PE f-line [Re: SexBurrito]
    #28013614 - 10/24/22 05:44 AM (2 years, 2 months ago)

If Fahtster says it works then believe him, he's the PE master.

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Invisiblebakedbeings
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Re: PE f-line [Re: DERRAYLD] * 1
    #28013669 - 10/24/22 06:39 AM (2 years, 2 months ago)

Quote:

DERRAYLD said:
If Fahtster says it works then believe him, he's the PE master.



:whathesaid:
there is much documented success


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Re: PE f-line [Re: SexBurrito]
    #28013924 - 10/24/22 11:34 AM (2 years, 2 months ago)

Quote:

SexBurrito said:
The pseudo case doesn't work well for PE in my experience. I did that to 8 PE sb's and they all blobed out.


Just cased these sweater boxes after full colonization with cvg and no more blobs. 






Did you do the full 2” of compressed layer?  Looks a little thin.  It really needs to be 2+” thick.  But yeah there’s lots of others who’ve done it.  I understand for “you” it didn’t work.. we should figure out why and get ya set right.  If you did go 2”, you may have to go a bit thicker with that specific culture.  Or try the peat casing… I’ve never not had that work with the 1” non compressed peat layer. 

As far as that cv @ 100% goes… have you dug down a bit to make sure there isn’t blobs under there?  If there isn’t and it’s about to throw up lots of pins, stick with that.. there’s no one way for everyone.. it’s more about finding what works for you.  That’s why there’s 8 different teks for the same thing in most cases

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Offlineschpat
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Re: PE f-line [Re: fahtster]
    #28013986 - 10/24/22 12:22 PM (2 years, 2 months ago)

Quote:

fahtster said:


Did you do the full 2” of compressed layer?  Looks a little thin.  It really needs to be 2+” thick.  But yeah there’s lots of others who’ve done it.  I understand for “you” it didn’t work.. we should figure out why and get ya set right.  If you did go 2”, you may have to go a bit thicker with that specific culture.  Or try the peat casing… I’ve never not had that work with the 1” non compressed peat layer. 

As far as that cv @ 100% goes… have you dug down a bit to make sure there isn’t blobs under there?  If there isn’t and it’s about to throw up lots of pins, stick with that.. there’s no one way for everyone.. it’s more about finding what works for you.  That’s why there’s 8 different teks for the same thing in most cases




Faht, do you include the 2" compressed layer in your spawn ratio?


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Re: PE f-line [Re: schpat] * 1
    #28014007 - 10/24/22 12:33 PM (2 years, 2 months ago)

Hmm.. good question.  I don’t think I did.  Pretty sure I made the main portion of the sub at 1:3 and then added the 2” layer so I’d say that made it closer to 1:5-6

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InvisibleStipe-n CapMDiscord
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Re: PE f-line [Re: SexBurrito] * 1
    #28014215 - 10/24/22 02:59 PM (2 years, 2 months ago)

Quote:

SexBurrito said:
The pseudo case doesn't work well for PE in my experience. I did that to 8 PE sb's and they all blobed out.






I can see why it didn't work just by looking at your picture; you're running a shallow shoebox that looks at best half full.

PE blobs will be the result of

1. Spawn ratio too high
2. Genetics
3  Surface conditions.

If you have a culture that readily blobs you'll need to use a thick pseudo-casing which sits above a compressed substrate. The compression layer between the substrate and the cv pseudo-casing produces a separation between the two layers, if you don't do this then all you have is one continuous substrate without a  pseudo-casing.

Your substrate does not have a 1.5-2' pseudo-casing, otherwise the shoebox would have more depth.

I run all substrates including shoeboxes at a 1:6 (this includes the pseudo-casing material) spawn to sub ratio which takes care of reason 1, separation of pseudo-casing layer by compression of sub and top layer separately takes care of reason 3, genetics are what they are.

Edited by Stipe-n Cap (10/24/22 03:41 PM)

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OfflineSexBurrito
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Re: PE f-line [Re: fahtster]
    #28014337 - 10/24/22 03:52 PM (2 years, 2 months ago)

Quote:

fahtster said:
Quote:

SexBurrito said:
The pseudo case doesn't work well for PE in my experience. I did that to 8 PE sb's and they all blobed out.


Just cased these sweater boxes after full colonization with cvg and no more blobs. 






Did you do the full 2” of compressed layer?  Looks a little thin.  It really needs to be 2+” thick.  But yeah there’s lots of others who’ve done it.  I understand for “you” it didn’t work.. we should figure out why and get ya set right.  If you did go 2”, you may have to go a bit thicker with that specific culture.  Or try the peat casing… I’ve never not had that work with the 1” non compressed peat layer. 

As far as that cv @ 100% goes… have you dug down a bit to make sure there isn’t blobs under there?  If there isn’t and it’s about to throw up lots of pins, stick with that.. there’s no one way for everyone.. it’s more about finding what works for you.  That’s why there’s 8 different teks for the same thing in most cases




So on the sweater boxes I did 2" at 100% but the sb's I did maybe a inch at spawn and both were pressed down. I'm definitely seeing better results imo with the case at 100% but that could just be my pseudo was to thin. Was getting super frustrated with all the blobs with the pseudo case.  In the future I will definitely case them again at 100 but I may try doing a thicker layer at spawn next time just to see if it works any better.  I haven't dug down at all but I did have a few more pins pop up while I was at work today but as of right now, no blobs.  Thanks for the suggestion fahtster, I'll give it a shot.  This is my first grow in 4 years so I'm all ears.


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InvisibleStipe-n CapMDiscord
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Re: PE f-line [Re: SexBurrito]
    #28014369 - 10/24/22 04:07 PM (2 years, 2 months ago)

You're supposed to add the pseudo-casing at spawning, adding it after 100% colonization is far too late.

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InvisibleStuey1
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Re: PE f-line [Re: Stipe-n Cap]
    #28016981 - 10/26/22 02:51 AM (2 years, 2 months ago)

I always case my PE tubs. But what do I know? I’m a little old school


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