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Enkidu
"No-Such-Person"


Registered: 07/09/16
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PE f-line
#28013189 - 10/23/22 08:07 PM (2 years, 2 months ago) |
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Is there anything I need to know for a grow using PE f-line ?
I've never grown PE before, only ever cubensis
I have PE f-line on agar as well as PE
-------------------- Within You , Without You
      
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,716
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Re: PE f-line [Re: Enkidu] 1
#28013285 - 10/23/22 09:12 PM (2 years, 2 months ago) |
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Got pics of the f-line? Never heard of it.
Thick 2” compressed top layer for regular PE for sure. That’s here
You can also case with peat/verm but that also needs pasteurizing. ^^^the pseudo layer works well and just uses more CV. PE is a cubensis btw
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Enkidu
"No-Such-Person"


Registered: 07/09/16
Posts: 10,704
Last seen: 2 months, 5 days
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Right, guess I meant I've never worked with any PE varieties and only regular cebes
I knew some PE called for the casing but I thought some you could just run with a top layer at spawn
Appreciate the link, will go that route and take your advice
No pics, idk much about PE varieties, someone sent me swabs or prints I don't even remember now, but that was what the label said
-------------------- Within You , Without You
      
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
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Re: PE f-line [Re: Enkidu]
#28013477 - 10/24/22 01:02 AM (2 years, 2 months ago) |
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The added top layer on your substrate is a casing: https://www.shroomery.org/13871/Casing
Never heard of "F line" but it sounds like a name given to track cloning/cultures by the original grower perhaps
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Egon_Spengler
Ever lurkin never workin



Registered: 04/02/22
Posts: 2,294
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My guess is that if no pics exist, or history differentiating this culture from any other PE out there, then the source you received your spores from had likely germinated a plate, taken 6 (or more) transfers from germination, labeled them A-F (and so on) to keep them separate in their records while growing out and fruiting multiple cultures in order to chase down a “line” worth cloning/printing/swabbing.
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SexBurrito
Wontons and WAP



Registered: 09/08/22
Posts: 322
Loc: United States
Last seen: 3 months, 15 days
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The pseudo case doesn't work well for PE in my experience. I did that to 8 PE sb's and they all blobed out.

Just cased these sweater boxes after full colonization with cvg and no more blobs.
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GYT with SexBurrito
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DERRAYLD
Constructus



Registered: 05/13/02
Posts: 12,101
Loc: South Africa
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If Fahtster says it works then believe him, he's the PE master.
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bakedbeings
orbiter of truth


Registered: 09/01/20
Posts: 4,563
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Quote:
DERRAYLD said: If Fahtster says it works then believe him, he's the PE master.
 there is much documented success
-------------------- Confused? Well now you can!
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,716
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Quote:
SexBurrito said: The pseudo case doesn't work well for PE in my experience. I did that to 8 PE sb's and they all blobed out.

Just cased these sweater boxes after full colonization with cvg and no more blobs.

Did you do the full 2” of compressed layer? Looks a little thin. It really needs to be 2+” thick. But yeah there’s lots of others who’ve done it. I understand for “you” it didn’t work.. we should figure out why and get ya set right. If you did go 2”, you may have to go a bit thicker with that specific culture. Or try the peat casing… I’ve never not had that work with the 1” non compressed peat layer.
As far as that cv @ 100% goes… have you dug down a bit to make sure there isn’t blobs under there? If there isn’t and it’s about to throw up lots of pins, stick with that.. there’s no one way for everyone.. it’s more about finding what works for you. That’s why there’s 8 different teks for the same thing in most cases
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schpat
psychesomadelic



Registered: 09/26/21
Posts: 1,041
Loc: South Africa
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Quote:
fahtster said:
Did you do the full 2” of compressed layer? Looks a little thin. It really needs to be 2+” thick. But yeah there’s lots of others who’ve done it. I understand for “you” it didn’t work.. we should figure out why and get ya set right. If you did go 2”, you may have to go a bit thicker with that specific culture. Or try the peat casing… I’ve never not had that work with the 1” non compressed peat layer.
As far as that cv @ 100% goes… have you dug down a bit to make sure there isn’t blobs under there? If there isn’t and it’s about to throw up lots of pins, stick with that.. there’s no one way for everyone.. it’s more about finding what works for you. That’s why there’s 8 different teks for the same thing in most cases
Faht, do you include the 2" compressed layer in your spawn ratio?
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,716
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Re: PE f-line [Re: schpat] 1
#28014007 - 10/24/22 12:33 PM (2 years, 2 months ago) |
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Hmm.. good question. I don’t think I did. Pretty sure I made the main portion of the sub at 1:3 and then added the 2” layer so I’d say that made it closer to 1:5-6
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Stipe-n Cap
The Pride of Cucamonga


Registered: 08/04/12
Posts: 8,259
Loc: Canada
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Quote:
SexBurrito said: The pseudo case doesn't work well for PE in my experience. I did that to 8 PE sb's and they all blobed out.

I can see why it didn't work just by looking at your picture; you're running a shallow shoebox that looks at best half full.
PE blobs will be the result of
1. Spawn ratio too high 2. Genetics 3 Surface conditions.
If you have a culture that readily blobs you'll need to use a thick pseudo-casing which sits above a compressed substrate. The compression layer between the substrate and the cv pseudo-casing produces a separation between the two layers, if you don't do this then all you have is one continuous substrate without a pseudo-casing.
Your substrate does not have a 1.5-2' pseudo-casing, otherwise the shoebox would have more depth.
I run all substrates including shoeboxes at a 1:6 (this includes the pseudo-casing material) spawn to sub ratio which takes care of reason 1, separation of pseudo-casing layer by compression of sub and top layer separately takes care of reason 3, genetics are what they are.
Edited by Stipe-n Cap (10/24/22 03:41 PM)
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SexBurrito
Wontons and WAP



Registered: 09/08/22
Posts: 322
Loc: United States
Last seen: 3 months, 15 days
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Quote:
fahtster said:
Quote:
SexBurrito said: The pseudo case doesn't work well for PE in my experience. I did that to 8 PE sb's and they all blobed out.

Just cased these sweater boxes after full colonization with cvg and no more blobs.

Did you do the full 2” of compressed layer? Looks a little thin. It really needs to be 2+” thick. But yeah there’s lots of others who’ve done it. I understand for “you” it didn’t work.. we should figure out why and get ya set right. If you did go 2”, you may have to go a bit thicker with that specific culture. Or try the peat casing… I’ve never not had that work with the 1” non compressed peat layer.
As far as that cv @ 100% goes… have you dug down a bit to make sure there isn’t blobs under there? If there isn’t and it’s about to throw up lots of pins, stick with that.. there’s no one way for everyone.. it’s more about finding what works for you. That’s why there’s 8 different teks for the same thing in most cases
So on the sweater boxes I did 2" at 100% but the sb's I did maybe a inch at spawn and both were pressed down. I'm definitely seeing better results imo with the case at 100% but that could just be my pseudo was to thin. Was getting super frustrated with all the blobs with the pseudo case. In the future I will definitely case them again at 100 but I may try doing a thicker layer at spawn next time just to see if it works any better. I haven't dug down at all but I did have a few more pins pop up while I was at work today but as of right now, no blobs. Thanks for the suggestion fahtster, I'll give it a shot. This is my first grow in 4 years so I'm all ears.
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GYT with SexBurrito
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Stipe-n Cap
The Pride of Cucamonga


Registered: 08/04/12
Posts: 8,259
Loc: Canada
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You're supposed to add the pseudo-casing at spawning, adding it after 100% colonization is far too late.
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Stuey1
We're all mad Here



Registered: 11/28/13
Posts: 740
Loc: Bat Country
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I always case my PE tubs. But what do I know? I’m a little old school
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