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Offlineklekle
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Red contamination on PF jars
    #28012316 - 10/23/22 11:11 AM (2 years, 2 months ago)

I've inoculated 10 PF jars with a spore syringe 13 Days ago. Today I checked and all of the jars had Red/Purple spots where I put the spore solution. The first jar I inoculated had no growth at all because I forgot to shake the syringe.

Im assuming that this means my jars are contaminated due to my syringe.

I PCed all of the jars and threw the cakes in the trash all but one ar to see what will happen.

When I inoculated I used only a drop or two.

I ordered two new syringes and am planning on inoculating my jars with the old syringe but using 1 ml per hole. Can this work? I mean is more solution possible to give the cubes enough head start so it won't turn purple again?


This is what the jars looked like on the sides where I didn't inoculate. Is this the mycelium I was looking for?


This is what the inoculation point looked like
I've only found posts about lipstick mold which didn't quite looked like my jars.


I've read that the cubes can outcompete lipstick mold but using contaminated jars would be a bad idea because the spores could contaminate my work area so I decided against it. Although i am planning on burying the leftover jar in my yard.

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OfflineJaksavage
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Re: Red contamination on PF jars [Re: klekle]
    #28012376 - 10/23/22 11:47 AM (2 years, 2 months ago)

Welcome

Many times a contam jar will still yield fruit.

You can fruit them in individual qt jars or ziplocs.

:goodluck:


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Invisiblebigfootscreepyuncl
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Re: Red contamination on PF jars [Re: klekle]
    #28012378 - 10/23/22 11:48 AM (2 years, 2 months ago)

Neither of those jars look good. Did you order your syringes from one of the forums trusted vendors?

A lot of people throw around the saying of 'mushroom mycelium can outcompete mold/bacteria' but the problem with that is that even if your mushroom mycelium manages to colonize faster than the mold or bacteria, there is still a contamination in there no matter what, and it is just a matter of time before it rears its ugly head. Mushroom mycelium is not parasitic and does not have the ability to beat contaminations into submission. You may get some fruits out of a contaminated jar, but at the end of the day contaminated is contaminated.

By using more than a couple drops it will likely improve the speed of germination and possibly accelerate the growth of the mycelium, but the syringes themselves are carrying contaminants.


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Offlineklekle
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Re: Red contamination on PF jars [Re: bigfootscreepyuncl]
    #28012391 - 10/23/22 11:55 AM (2 years, 2 months ago)

I just looked it up and it turns out that the website I ordered from is a trusted vendor.

Can you specify what you mean by the jars not looking good? Do you think the white stuff is mycelium or could it be all mold

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Offlineklekle
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Re: Red contamination on PF jars [Re: Jaksavage]
    #28012393 - 10/23/22 11:57 AM (2 years, 2 months ago)

Thanks ^^

Im a bit worried about the spores of the contams spreading and ruining future operations. And I'm not sure if there is even cubensis mycelium in the jars or if it's all mold :confused: :thumbdown:

Edited by klekle (10/23/22 11:57 AM)

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Invisiblebigfootscreepyuncl
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Re: Red contamination on PF jars [Re: klekle]
    #28012407 - 10/23/22 12:09 PM (2 years, 2 months ago)

It may just be the picture quality but the mycelium looks kinda chalky/powdery. To me, from the pictures you included, it looks mostly like mold. But like I said earlier, it doesn't really matter if its mushroom or mold mycelium since there is actively sporulating mold in there. Meaning, it's done for anyway :shrug:

Don't worry so much about these attempts ruining future grows. There are billions of mold spores floating around in the air you breathe. Just take the jars outside to open and dispose of them, wash the jars and lids with hot soapy water and move on to your next attempt.

If you walk us through your process we can help narrow down what went wrong (assuming it's not just a dirty syringe). Did you use a SAB? How long did you PC them, and at what psi? Did you vent your PC? The more info you provide the easier it will be for us to help you


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Offlineklekle
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Re: Red contamination on PF jars [Re: bigfootscreepyuncl]
    #28012449 - 10/23/22 12:45 PM (2 years, 2 months ago)

Of course I was hoping that this would work out right away but I'm not surprised that I've ran into trouble on my first try. I hope I didn't forget anything

My PC reaches 13,77 psi. It was advertised with 15 but the manual says 95kPa.I PC for 45 minutes. Does venting the PC mean to let the pressure go all the way down before opening it? I do wait for it.

I use a SAB. I spray soapy water on the table and the walls but not on the ceiling. I clean the walls and the table with 70% ISO before. I place the jars on the table and then put the SAB over them.

I cleaned the closet where the jars sit in too and they are in the top shelf so they are far from the floor.

When the jars come out of the PC I use a tong that I wiped with Iso to move the hot jars in the closet I remove the foil (2 layers) once they cooled down enough to touch them. I wipe my hands and arms with Iso too

My jars have a verm layer my verm is 3-6mm

I wear a mask while handling the jars from the point I take of the foil.

I inoculate the next day I heat the needle with a blowtorch I pull in some hot air one time and shake the syringe. I angle the syringe through the holes and squeeze out some liquid at about the middle height of the jar. I reheat the needle every 2 jars. My jars have 2 holes

I carefully put the jars inside the closet. I've had them in darkness the first couple of days but left the door open afterwards

Temperature is 20-23°C

Edited by klekle (10/23/22 12:47 PM)

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OfflineLogicaL ChaosM
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Re: Red contamination on PF jars [Re: klekle] * 1
    #28012469 - 10/23/22 12:56 PM (2 years, 2 months ago)

Damn, looks like a red mold to me. That jar is no good but you can try fruit it in isolation :shrug:

If the syringes are infected with mold spores, using more solution per hole wouldnt help except for seeing mold grow faster.

Id recommend getting agar to test your syringes cleanliness or trying a different syringe vendor. It could also be your technique in inoculating them. What sort of SAB do you have? Can you describe your clean process for inoculating?


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Invisiblebigfootscreepyuncl
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Re: Red contamination on PF jars [Re: klekle]
    #28012510 - 10/23/22 01:20 PM (2 years, 2 months ago)

Quote:

klekle said:
Of course I was hoping that this would work out right away but I'm not surprised that I've ran into trouble on my first try. I hope I didn't forget anything

My PC reaches 13,77 psi. It was advertised with 15 but the manual says 95kPa.I PC for 45 minutes. Does venting the PC mean to let the pressure go all the way down before opening it? I do wait for it.

Venting means to put the lid on the PC and lock it into place (without the jiggle weight) and allow it to blow out steam for 10-15 minutes prior to allowing it to build up pressure. You ALWAYS need to vent and ALWAYS allow your PC to completely depressurize before opening the lid. I can not stress that enough. DO NOT open the PC while it is pressurized!

I use a SAB. I spray soapy water on the table and the walls but not on the ceiling. I clean the walls and the table with 70% ISO before. I place the jars on the table and then put the SAB over them.

I cleaned the closet where the jars sit in too and they are in the top shelf so they are far from the floor.

When the jars come out of the PC I use a tong that I wiped with Iso to move the hot jars in the closet I remove the foil (2 layers) once they cooled down enough to touch them. I wipe my hands and arms with Iso too

My jars have a verm layer my verm is 3-6mm

I wear a mask while handling the jars from the point I take of the foil.

I inoculate the next day I heat the needle with a blowtorch I pull in some hot air one time and shake the syringe. I angle the syringe through the holes and squeeze out some liquid at about the middle height of the jar. I reheat the needle every 2 jars. My jars have 2 holes

Heat the needle to red hot and squirt some spore solution through the needle to cool it. Sucking air into the syringe is a huge vector for contamination.

I carefully put the jars inside the closet. I've had them in darkness the first couple of days but left the door open afterwards

Mushrooms need light. Don't worry about keeping them in the dark. Ambient light will be sufficient while you're getting started.

Temperature is 20-23°C





Everything else sounds OK to me, but if your syringe is contaminated there's nothing you can do unless you were to clean it up on agar :shrug:


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OfflineLogicaL ChaosM
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Re: Red contamination on PF jars [Re: klekle]
    #28012520 - 10/23/22 01:25 PM (2 years, 2 months ago)

Yeah i think the pulling in air into the syringe part could be the issue. Never do that! Push on the plunger only. Never pull back during/in-between inoculation.

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Offlineklekle
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Re: Red contamination on PF jars [Re: bigfootscreepyuncl]
    #28012524 - 10/23/22 01:26 PM (2 years, 2 months ago)

for cooling down do I squirt the drop outside the jar or inside? So I don't need to shake it? Or can I shake it without air inside? Ive read that you need the air for that. I'll not suck air into my next syringes. Thanks for your effort 🙏🏼

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OfflineLogicaL ChaosM
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Re: Red contamination on PF jars [Re: klekle]
    #28012531 - 10/23/22 01:29 PM (2 years, 2 months ago)

Yeah squirt outside of a jar. Its waste. Shake before heating up the tip. Heating is last. U dont need air in the syringe to shake it up.

Ive been having good sucess with no heat, hydrogen peroxide wiping on my syringe tips. Could be Im finally getting good at sterile technique. Its hard to say. Might be worth a try :thumbup:

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