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InvisibleHumbleDragon
Stranger
I'm a teapot User Gallery

Registered: 04/19/22
Posts: 50
New Season - Fresh Start / Advice Requested
    #28011990 - 10/23/22 06:07 AM (2 years, 2 months ago)

Hello friends, I have returned after combating a major contamination issue that nearly crushed all my hopes and dreams. Lesson learned - take your time and don't allow room for mistakes, even if that means you have to do everything yourself.

That being said, I have a few questions that are somewhat vague - I have a pretty good idea of what I am going to do this time around but I am curious as to what some others might do in my shoes.

A bit of background - I picked up mycology this year, a friend gave me a bunch of equipment because I was moving into a house with a basement perfect for the hobby. He has had many successful grows in the past and offered to teach me since I knew absolutely nothing. He gave me just about everything you could ask for minus some petri dishes that were clean. I spent several months learning agar work assuming I was failing with my sterile technique before he finally found the time to come and see what the issue was and realized everything he gave me was contaminated in one way or another. I started isolating what I did have away while waiting for some new genetics from him, he never delivered and somewhat vanished. I still have the equipment but no guide :laugh:

Long story short I ended up having to cleanse my space and call the summer a wash. Spores are hard to come by where I live and so it took awhile to come up with a method to acquire some. Tomorrow I get 8 different syringes from one of our lovely vendors and I am beyond excited to start over fresh with a relatively clean(I hope)grow environment.

So what I have to work with equipment wise are 3 massive grow tents(20x16x16/13x10x10*2) in a relatively clean basement(sprayed several times over with Sporicidin top to bottom)
I was using one of the smaller tents for my sterile lab work, the other for incubating jars(not as an incubator just as a space for them) with the goal to have the large tent be my growing space.

1 - 24x24 Laminar Flowhood
1 - 24x48 Hepa flowhood

60 Fresh petri dishes
20 Y Sectioned Dishes
Media Bottles & Wine bottles that pour extra nice
Agar - LME - ELME
Blender
Parafilm
All of the scalpels/unique transfer tools you can imagine
Torch/Iso/Gloves/Masks/Arm Covers etc

Dedicated minifridge for all of the new agar

pretty much everything I can think of for fleshing out these spores I built up over the year of trial and error. Which brings me to now -

I never actually did PF-Tek in all of my attempts this year - I figure since contamination was such an issue(though it was somewhat out of my hands as to why)that I should start there. Should I MSS right into the jars and do my agar work as a separate project? I'm pretty confident in my lab work but due to the setbacks I've had so far I want to hit this from all angles and really make sure I have at least something that will yield perfectly clean spawn by the time spring starts to hit.


I'm sure theres more I should say but thats about all I can think of at the moment. Any input is greatly appreciated, I'm currently cleaning/rewiring the hepa flowhood so it can start cleaning the air downstairs but I plan to spend most of the day making agar until I get a batch thats crystal clear. I want to have plates ready to go the moment the spores arrive xD

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Offlineechochild
The Padawan Learner

Registered: 08/26/22
Posts: 226
Last seen: 2 years, 2 months
Re: New Season - Fresh Start / Advice Requested [Re: HumbleDragon]
    #28011996 - 10/23/22 06:28 AM (2 years, 2 months ago)

So I don't know how the veteran growers are going to respond (I do have some questions myself on some of what you said but I'll hold them for now), but as a now slightly experienced noob, this is my view:

I shotgunned several Teks when I started because I could. I did multiple just to see what works best in my environment, for me, and that I expected a minimum contam rate of 50% (if not more) based on the nature of the game and inexperience.

With your MS coming in, if I were in your shoes, I would take 2 drops for each MS and apply it to 3 agar plates each. Then, I would get into reading the updated PF Tek and innoc a few jars, since PF Tek is the most forgiving of contam from MS. I'd save a little from each MS, just in case all the agar plates of that strain became bacterial or such, just to have some back ups.

These are the threads that have helped me the most:
https://www.shroomery.org/forums/showflat.php/Number/24144021 (Getting Started)
https://www.shroomery.org/forums/showflat.php/Number/24179086 (Updated PF Tek)
https://www.shroomery.org/forums/showflat.php/Number/26601054/fpart/1/vc/1 (Water Tub Tek)

Remember... your basement can be the cleanest clean that ever cleaned... but bacteria and mold could be riding along with the MS's themselves. So I would practice the agar isolation of good / clean agar plates while the PF Tek is starting to roll.

The PF Tek could be a good jumping point later for clone specimens.

I wish you luck!

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Invisiblebakedbeings
orbiter of truth
 User Gallery

Registered: 09/01/20
Posts: 4,563
Trusted Cultivator
Re: New Season - Fresh Start / Advice Requested [Re: echochild] * 1
    #28012022 - 10/23/22 07:02 AM (2 years, 2 months ago)

Quote:

1 - 24x24 Laminar Flowhood
1 - 24x48 Hepa flowhood



what does this mean? one is hepa but not laminar and one is laminar but not hepa?

Quote:

3 massive grow tents(20x16x16/13x10x10*2) in a relatively clean basement(



how fucking big is your basement??

i didnt see mention of a pressure cooker

one syringe goes a long way and seeing as they are hard to get and you havent had a ton of success yet, i recommend picking one as your "learning syringe", putting the rest in the fridge, and using it to knock up lots of agar plates (1 drop each) and a handful of PF jars. that will get you off to a good start

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