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Offlinestemseed
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Registered: 10/10/22
Posts: 73
Last seen: 1 month, 29 days
P Nat on Agar
    #28011567 - 10/22/22 10:05 PM (2 years, 2 months ago)

Got some P Nats into this no pour Pyrex media container. Remind me to do pours only from now on. I should have listened to the old timers when they said no pours suck, because I see now that they do. I had tons of boil over and cleaning it up was a pain and it may all contam. It’s looking rough for this one but I have several Petri’s going as well.

Agar + LME + Activated charcoal. Unscrewed the lid a bit and pressure cooked for 45mins. Flame sterilized my scalpel very badly with a bic lighter and did a t1 transfer in a small ass SAB to this. Then I screwed the lid very loosely and wrapped with parafilm.




This is all fictitious of course.

Edited by stemseed (12/09/22 12:08 AM)

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InvisibleFridgedoor
Psssssst!
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Registered: 09/13/20
Posts: 1,136
Re: P Nat on Agar [Re: stemseed]
    #28011583 - 10/22/22 10:14 PM (2 years, 2 months ago)

This is quite a Petri dish you got there :smile:. I imagine the transfer from T1 to T2 is going to be a little challenge.

Happy growing :mushroom2:


--------------------
Hokus Pokus Fidibus!

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OfflineCJD
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Registered: 08/10/22
Posts: 383
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Last seen: 27 days, 4 hours
Re: P Nat on Agar [Re: stemseed]
    #28011590 - 10/22/22 10:18 PM (2 years, 2 months ago)

Yeah I think with a 250ml bottle you could have just poured into smaller containers. What did you transfer from? How do you plan to make a transfer out of the bottle neck? I'm sure you'll get something good either way, just might not be easy to execute.

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