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OfflineTeaboomboom
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Registered: 10/12/22
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Can you ru-use an already inoculate grain spawn bag?
    #28011396 - 10/22/22 09:02 PM (2 years, 2 months ago)

So, i purchased these syringes from my local hydro store and upon inoculating 2 5lb grain spawn bags from Midwest Grow with 10cc of Jedi mind fuck and golden teachers. It’s been 4 days and no growth or even sign of it. I’m guessing maybe the spores required to meet in order for mycelium growth to happen, didn’t? However I have more syringes, can I go and inoculate another syringe of a different strain? I’m still a newbie, sorry if this a silly question. 🙏🏽

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Offlinerumfor69
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Registered: 08/05/11
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Re: Can you ru-use an already inoculate grain spawn bag? [Re: Teaboomboom]
    #28011417 - 10/22/22 09:09 PM (2 years, 2 months ago)

It takes way longer for spores to germinate on grain. Dont even start to wonder about it untill 2 weeks


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OfflineRusty2096
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Registered: 08/23/22
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Re: Can you ru-use an already inoculate grain spawn bag? [Re: Teaboomboom]
    #28011420 - 10/22/22 09:09 PM (2 years, 2 months ago)

This is extremely silly but I'll try to help.

First of, MSS to grain is always a bad plan.

Second, spores in a bag can take weeks to germinate and show growth.

Third, the more you inject, the more contam you will introduce. You bags are very likely already fucked so don't add any more.

Couple reads to help you get started:
Start by reading this
Then do PF Tek
And fruit in a water tub

Welcome to the Shroomery and goodluck!


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Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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OfflineSo Anyway
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Registered: 07/11/21
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Last seen: 18 days, 16 hours
Re: Can you ru-use an already inoculate grain spawn bag? [Re: Teaboomboom] * 1
    #28011424 - 10/22/22 09:10 PM (2 years, 2 months ago)

4 days is nothing for germinating spores, it can take up to 10+ to see growth. Putting a spore syringe directly to grain is bad mmkay. Maybe you'll get lucky...back before mss to grain was a faux pas around here I did it a bunch and admittedly got some of my best canopies from it. 
:homerlurk:

Best practice is to germinate spores on agar and transfer to grain.

If you inject grain and don't see growth, injecting again is not a great idea; likely bacteria prevented myc from growing


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So anyway, uh...

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