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InvisibleSuckatshrooms
Seriously, don’t listen to me.
Female

Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
Sterile spore print to agar.
    #28010474 - 10/22/22 09:58 AM (2 years, 2 months ago)

Have any of you made a sterile spore print straight to agar? Let an agar plate fruit, cut the cap off and place directly on another plate to print directly onto the agar?

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OnlineDERRAYLD
Constructus
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Registered: 05/13/02
Posts: 12,101
Loc: South Africa
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Re: Sterile spore print to agar. [Re: Suckatshrooms]
    #28010480 - 10/22/22 10:01 AM (2 years, 2 months ago)

Why?

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Invisibleruawakeyet
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Registered: 05/03/21
Posts: 1,862
Re: Sterile spore print to agar. [Re: DERRAYLD]
    #28010484 - 10/22/22 10:07 AM (2 years, 2 months ago)

If it could be done, that would likely produce the world's smallest spore print.

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InvisibleSuckatshrooms
Seriously, don’t listen to me.
Female

Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
Re: Sterile spore print to agar. [Re: DERRAYLD]
    #28010490 - 10/22/22 10:21 AM (2 years, 2 months ago)

Quote:

DERRAYLD said:
Why?



I have no idea. Maybe a tiny shroom farm for a doll house?

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OfflineHappyHigh
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Registered: 05/05/17
Posts: 905
Last seen: 3 months, 25 days
Re: Sterile spore print to agar. [Re: ruawakeyet] * 1
    #28010492 - 10/22/22 10:22 AM (2 years, 2 months ago)

Why not just cut some of the first and place it on the 2nd, the spores are just doing the same exact thing over again.
The only reason too do something like you are saying is too get 100% clean spore print then stop.
you would be going back a step, not moving forward.


--------------------
Had I the heavens' embroidered cloths,
Enwrought with golden and silver light,
The blue and the dim and the dark cloths
Of night and light and the half light,
I would spread the cloths under your feet:
But I, being poor, have only my dreams;
I have spread my dreams under your feet;
Tread softly because you tread on my dreams.

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InvisibleSuckatshrooms
Seriously, don’t listen to me.
Female

Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
Re: Sterile spore print to agar. [Re: HappyHigh]
    #28010543 - 10/22/22 11:23 AM (2 years, 2 months ago)

Quote:

HappyHigh said:
Why not just cut some of the first and place it on the 2nd, the spores are just doing the same exact thing over again.
The only reason too do something like you are saying is too get 100% clean spore print then stop.
you would be going back a step, not moving forward.



I was under the impression that cloning over and over again degraded the genetics until eventually it wouldn’t be able to reproduce.

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Invisibleruawakeyet
 User Gallery

Registered: 05/03/21
Posts: 1,862
Re: Sterile spore print to agar. [Re: Suckatshrooms]
    #28010554 - 10/22/22 11:31 AM (2 years, 2 months ago)

Quote:

Suckatshrooms said:
Quote:

HappyHigh said:
Why not just cut some of the first and place it on the 2nd, the spores are just doing the same exact thing over again.
The only reason too do something like you are saying is too get 100% clean spore print then stop.
you would be going back a step, not moving forward.




I was under the impression that cloning over and over again degraded the genetics until eventually it wouldn’t be able to reproduce.




I'm confused, are you talking about cloning or printing?

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InvisibleSuckatshrooms
Seriously, don’t listen to me.
Female

Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
Re: Sterile spore print to agar. [Re: ruawakeyet]
    #28010595 - 10/22/22 12:02 PM (2 years, 2 months ago)

Quote:

ruawakeyet said:
Quote:

Suckatshrooms said:
Quote:

HappyHigh said:
Why not just cut some of the first and place it on the 2nd, the spores are just doing the same exact thing over again.
The only reason too do something like you are saying is too get 100% clean spore print then stop.
you would be going back a step, not moving forward.




I was under the impression that cloning over and over again degraded the genetics until eventually it wouldn’t be able to reproduce.




I'm confused, are you talking about cloning or printing?



Sorry, I know jack shit about this process so I may just be using incorrect terminology.

I thought that you were talking about cloning from one plate to another instead of the hypothetical tiny spore print. My understanding is that cloning/mycelium plate transfers can’t be done indefinitely due to genetics degrading, but selecting fruits and using spores can be done forever.

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OfflineLogicaL ChaosM
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Re: Sterile spore print to agar. [Re: Suckatshrooms] * 1
    #28010615 - 10/22/22 12:13 PM (2 years, 2 months ago)

You can make pretty clean spore prints by following teks on here. I wouldnt bother with making a tiny spore print from a fruiting agar dish.

My favorite tek is by eatualive. I follow his pretty closely: https://www.shroomery.org/forums/showflat.php/Number/24732724#24732724

Its so clean, I can make spore syringes out of them with virtually no molds, which is perfect for me :awesomenod:


--------------------
"The beginnings of unlimited energy will likely emerge around 2024 and will become a worldwide manifestation by 2040. Once unlimited energy is established, the possibilities will be endless - your progressive movement will accelerate to astronomical heights." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

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InvisibleShrimps
Traveler
I'm a teapot


Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
Re: Sterile spore print to agar. [Re: LogicaL Chaos] * 2
    #28010649 - 10/22/22 12:33 PM (2 years, 2 months ago)

Quote:

LogicaL Chaos said:


My favorite tek is by eatualive. I follow his pretty closely: https://www.shroomery.org/forums/showflat.php/Number/24732724#24732724






My bookmarks just get longer and longer <3


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
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Proper Surface Moisture
Recognizing and dealing with contamination

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OfflineLand TroutM
Stranger
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Registered: 01/08/18
Posts: 3,180
Last seen: 6 hours, 20 minutes
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Re: Sterile spore print to agar. [Re: Suckatshrooms] * 1
    #28010658 - 10/22/22 12:37 PM (2 years, 2 months ago)

Quote:

Suckatshrooms said:
Quote:

HappyHigh said:
Why not just cut some of the first and place it on the 2nd, the spores are just doing the same exact thing over again.
The only reason too do something like you are saying is too get 100% clean spore print then stop.
you would be going back a step, not moving forward.



I was under the impression that cloning over and over again degraded the genetics until eventually it wouldn’t be able to reproduce.



I’m not too familiar with anyone cloning clones to the point of degradation, not that it isn’t possible.  Lots of people have come up with good cultures from cloning clones of clones, sometimes it doesn’t go as well like I have a clone that makes a weaker cake than the MS it came from but that’s got more to do with luck of the draw.
And to answer your first question.  Yes, if your culture fruits on agar and produces fertile spores you can swab/streak, those to another plate,  if you put the cap to “print” on the second plate it would more likely clone to it faster than the spores would germinate.  Transferring pins from a plate are a great way to transfer.  Lots of people do that.

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Invisibleruawakeyet
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Registered: 05/03/21
Posts: 1,862
Re: Sterile spore print to agar. [Re: Land Trout] * 1
    #28010836 - 10/22/22 02:34 PM (2 years, 2 months ago)

Land Trout, I wish I could explain things as well as you just did.

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