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Myyco.com Shop: Golden Teacher Liquid Culture For Sale, Isolated Cubensis Liquid Culture For Sale, Penis Envy Liquid Culture For Sale

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OfflinePadaone
Mycophage

Registered: 07/03/22
Posts: 68
Last seen: 3 months, 27 days
Re: Super cloudy LC. What did I do wrong? [Re: No-Pilot-555] * 1
    #28009820 - 10/21/22 06:54 PM (2 years, 2 months ago)

PGT...there is your problem. You literally dont need anything more than water and LME. Muy complicado.

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Offlinesmalltalk_canceled
Babnik
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Registered: 07/13/20
Posts: 3,174
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Re: Super cloudy LC. What did I do wrong? [Re: No-Pilot-555] * 1
    #28009822 - 10/21/22 06:55 PM (2 years, 2 months ago)

how dare you link youtube on shroomery, guards!

guards!



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Edited by smalltalk_canceled (10/21/22 06:55 PM)

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Offlinematsc
Stranger
Male

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Registered: 09/17/12
Posts: 681
Loc: Arizona
Last seen: 1 year, 2 months
Re: Super cloudy LC. What did I do wrong? [Re: smalltalk_canceled]
    #28009997 - 10/21/22 09:45 PM (2 years, 2 months ago)

So, I looked up the dudes video (I assume this is based on his "Super" liquid culture recipe). 12ml corn syrup in 600ml water (or 20ml in a liter) is a 2% v/v solution, that's nothing out of the ordinary. The 1.2g of malt and 1 gram of peptone are .12% and .1% (w/v) respectively, also not unusual.

So, yeah, his recipe isn't really super incredibly high nutrient. That said, it's still overkill for what most amateur mycologists need unless you're getting into really niche stuff. Yeah you're adding a bit of nitrogen and some micronutrients in the mix, but, general saprobes aren't that picky in the short term.

In your particular case, it's almost assuredly the yeast that is causing the cloudiness. Maybe your malt extract was a bit starchy, or you caramelized things slightly, but whole yeast isn't exactly soluble, so I'd point there first.


It's not really going to hurt anything, any fungi that go to town in there will eventually eat up that excess chitin in the mix. It may not look as pretty, and the cloudiness might make spotting contaminants a wee bit trickier, but that's about the worst of it. Also, your mixes are at 3.33% sugar for the honey, and 3% for the corn syrup, which is starting to push things a bit (I usually stick around 1-1.5% myself) but you're not really in a problem range yet (again, unless you're looking at niche stuff). Just a little overkill, but not catastrophically. 


(and really, malt extact on its own is plenty for general saprobes. Maybe you'll have to wait a day or two longer to get to a certain cell density in the mix compared to the video's recipe, but no need to stress about it)


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OfflineNo-Pilot-555
Astral Traveler
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Registered: 09/30/22
Posts: 102
Loc: Outer Space's Ceiling
Last seen: 6 months, 5 days
Re: Super cloudy LC. What did I do wrong? [Re: matsc]
    #28010329 - 10/22/22 07:34 AM (2 years, 2 months ago)

Thank you for this. I've just learned a lot from PGT but in my opinion I like Gary from fresh from the farm fungi. He's more sanitary about his stuff.

Either way, any time I have used what I've learned in my own lab, I tend to end up messing it up a long the way.

I do think my problem is the yeast and I will be getting peptone this upcoming Wednesday so, I'll cut out the yeast and try lowering the other nutrients a bit more.

Thank you for the help! Shroomery is literally the best source for mycology. I love it.


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Knowledge above all none sense.

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Myyco.com Shop: Golden Teacher Liquid Culture For Sale, Isolated Cubensis Liquid Culture For Sale, Penis Envy Liquid Culture For Sale


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