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OfflineTheUsualSuspect
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Registered: 09/11/22
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cobweb mold - i dont get it *DELETED*
    #28008535 - 10/20/22 10:36 PM (2 years, 2 months ago)

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OfflineBagOfDicks
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Registered: 12/11/21
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Re: cobweb mold - i dont get it [Re: TheUsualSuspect]
    #28008546 - 10/20/22 11:10 PM (2 years, 2 months ago)

Well that's pretty good rates of contam vs axenic.  Are you flaming your tools to sterilize them? Or using heat? 

Regardless--1 out of 10 is not bad at all.  And my rates are not that good.


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InvisibleNichrome
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Registered: 12/17/18
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Re: cobweb mold - i dont get it [Re: TheUsualSuspect] * 1
    #28008548 - 10/20/22 11:17 PM (2 years, 2 months ago)

Quote:

TheUsualSuspect said:
About 4 days ago I ran some oats in the pressure cooker. Was trying the soak and scoop method instead of my usual no soak. I did 10 2 quart bags and the next day I hit them with liquid culture. The liquid culture is from a 1/2 gallon batch I have been using for last month or so, 10cc per bag using a livestock irrigation gun. 9 of the 10 bags are showing predictable decent growth. Bag 10 shows prominent cobweb contamination (which is the first time ive run into it).





10 bags of soaked and pc oats processed exact same way, soaked then scooped, 2 hours at 15psi after hitting temp with steam vented. Same jar of LC. All done in front of the flow hood. All tools hit well with 70% iso prior to starting. One bag contaminates, 9 dont. Wierd.




This is likely where you are going wrong. You need to use sterile tools for inoculation, not sanitized tools. If you going to use a noc gun (99.9% of cultivators don't) you need one that you can run through the pc.

Have you tested your lc on agar? Was the LC made using a clean wedge of mycelium on agar or did you put spores in the fluid??? A half gallon is a lot and it's not commonly made in large batches like that. Large volumes like that are really easy to flub up and hard to handle physically.


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OfflineTheUsualSuspect
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Re: cobweb mold - i dont get it *DELETED* [Re: BagOfDicks]
    #28008550 - 10/20/22 11:19 PM (2 years, 2 months ago)

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InvisibleNichrome
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Registered: 12/17/18
Posts: 7,669
Loc: Zone 5
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Re: cobweb mold - i dont get it [Re: TheUsualSuspect] * 1
    #28008556 - 10/20/22 11:26 PM (2 years, 2 months ago)

There are a lot of steps from "boiled" to inoculating grain. I'd still suspect that's the culprit. Boiling in water is not a good sterilization method for tools used for sterile culture work.

Any tools that you can't flame sterilize, like inoculation guns or syringes, need to be run through a pressure cooker to be considered sterilized. That's why dentists and tattooists use PC's or autoclaves for all their tools and they don't just boil them in water which would be easier for sure. Boiled is not sterilized.

How did you make the LC?


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discussions are a healthy alternative to arguments

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OfflineTheUsualSuspect
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Registered: 09/11/22
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Re: cobweb mold - i dont get it *DELETED* [Re: Nichrome] * 1
    #28008558 - 10/20/22 11:31 PM (2 years, 2 months ago)

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OfflineJaksavage
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Registered: 11/19/21
Posts: 548
Loc: Left coast
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Re: cobweb mold - i dont get it [Re: TheUsualSuspect]
    #28012120 - 10/23/22 08:33 AM (2 years, 2 months ago)

That change in grain prep has my warning light going off.

:goodluck:


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OfflineKROM
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Re: cobweb mold - i dont get it [Re: Jaksavage]
    #28012152 - 10/23/22 08:51 AM (2 years, 2 months ago)

Two thoughts: 1) you might want to try a different oat prep. For instance, I like Bodโ€™s oat prep, with the only difference being I thoroughly wash the oats before boiling, as I get some dirty ass, dust bucket oats.
2) You might consider increasing your PC cycle time. P9 pcโ€™s 4 quart bags for 3.5 to 4 hours. Iโ€™m not sure how to approach it for 2 quart bags of oats, but I usually pc quart jars for 2.5 hours.


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