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OfflineMycoShadow
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Can you grow mycelium in LC from MSS?
    #28008529 - 10/20/22 10:26 PM (2 years, 2 months ago)

I am a bit confused as to whether or not you can inoculate into LC right from a MSS?  If so is there a specific type of LC or will any work?

Pretty sure you need to go to Agar first but I am a bit confused about this.  Thanks!!

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OfflineLogicaL ChaosM
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Re: Can you grow mycelium in LC from MSS? [Re: MycoShadow] * 3
    #28008533 - 10/20/22 10:35 PM (2 years, 2 months ago)

You should place multispores on agar first to verify its clean and/or to pick a healthy looking mycelium.

If you go spores to LC, you're likely to have an infected LC.


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Re: Can you grow mycelium in LC from MSS? [Re: LogicaL Chaos] * 1
    #28008584 - 10/21/22 12:00 AM (2 years, 2 months ago)

The other thing with LC is that because of the high volume that it leads to you might want to know more about the genetics you are working with.  It would not be good to make a bunch of spawn from LC that you don't know is going to be productive.


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OfflineMycoShadow
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Re: Can you grow mycelium in LC from MSS? [Re: schpat] * 1
    #28009354 - 10/21/22 02:06 PM (2 years, 2 months ago)

Ok got it that totally makes sense and I saw the flow chart posted on another thread.  Agar it is, going to see how well i can do without a flow hood and go from there. thanks ya'll have a great weekend!!

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OfflineLogicaL ChaosM
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Re: Can you grow mycelium in LC from MSS? [Re: MycoShadow]
    #28009372 - 10/21/22 02:19 PM (2 years, 2 months ago)

U must be talking about this flowchart made by Bodhi: https://www.shroomery.org/forums/showflat.php/Number/24420178#24420178

If u look closely, it says "(highly not recommended)". Its as risky as spores to grain, thus clean colonized agar is the best method for grain and LC inoculation.

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