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OfflineLetsgrowstones
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Tried Agar - anything save-able or should I toss? * 1
    #28007369 - 10/20/22 03:53 AM (2 years, 2 months ago)

Heya all you beautiful people,

I tried my hand at some old spores I had lying around, please take a look at the pictures and let me know what you think,
























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OfflineJaksavage
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Re: Tried Agar - anything save-able or should I toss? [Re: Letsgrowstones] * 1
    #28008977 - 10/21/22 08:42 AM (2 years, 2 months ago)

Start over.
I don't see any myc.

:goodluck:


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InvisibleSprores
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Registered: 09/24/19
Posts: 169
Re: Tried Agar - anything save-able or should I toss? [Re: Letsgrowstones]
    #28009120 - 10/21/22 11:13 AM (2 years, 2 months ago)

First plate has some kind of contam, second plate i don't see anything happening.

What is your agar recipe?

If using swabs, I would recommend streaking the plate in a zig-zag pattern. If using a print, I would recommend using an inoculation loop (you can DIY one of these easily) and streaking the plate in a zig-zag pattern. If using a spore syringe, I would recommend shaking up the syringe to break up any spore clumps, then squirting a small drop of solution onto the plate, and streaking the solution on the plate in a zig-zag pattern.

Of course, following sterile procedure is paramount to success. Flame sterilization, SAB, gloves, 70% iso, long sleeves, and making sure your hands are never hovering over an open plate are things you'll need to keep in mind.

For newbies I also recommend reading through all of Bod's and Pastywhyte's teks. See here.


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Offlinehazyhorse
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Re: Tried Agar - anything save-able or should I toss? [Re: Sprores]
    #28009302 - 10/21/22 01:26 PM (2 years, 2 months ago)

maybe i just have bad eyes but that first plate looks ok to me, just more tomentose. i’d take a transfer & see how it looks as it grows out more. second plate looks way more suspect. are the rest of them still blank? i don’t see anything on the others


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OfflineLogicaL ChaosM
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Re: Tried Agar - anything save-able or should I toss? [Re: Letsgrowstones]
    #28009316 - 10/21/22 01:34 PM (2 years, 2 months ago)

I definitely see tomentose mycelium. Some of the plates kinda look bacterial. I would let them grow out more.


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Offlinehazyhorse
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Re: Tried Agar - anything save-able or should I toss? [Re: LogicaL Chaos] * 1
    #28009322 - 10/21/22 01:38 PM (2 years, 2 months ago)

glad i wasn’t just seeing things lol. germ plates have a tendency to look super weird ime. after a transfer you should have a clearer picture of what the culture looks like


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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OfflineJaksavage
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Re: Tried Agar - anything save-able or should I toss? [Re: Letsgrowstones]
    #28009368 - 10/21/22 02:17 PM (2 years, 2 months ago)

I'm the one with bad eyes and quick draw opinions, LOL.

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Offlinehazyhorse
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Re: Tried Agar - anything save-able or should I toss? [Re: Jaksavage] * 1
    #28009371 - 10/21/22 02:19 PM (2 years, 2 months ago)

i think the amount of rhizomorphic agar porn that gets posted on here also skews people’s perception of what agar should look like. growth on agar is super variable & a lot of stuff is clean even if it’s not a perfect rhizo monoculture


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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OfflineLogicaL ChaosM
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Re: Tried Agar - anything save-able or should I toss? [Re: hazyhorse] * 1
    #28009868 - 10/21/22 07:36 PM (2 years, 2 months ago)

Bingo!

Its also noteworthy to mention that molds typically have a tomentose appearance and thus cubenis tomentose mycelium can be misidentified as a mold.

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Offlinehazyhorse
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Re: Tried Agar - anything save-able or should I toss? [Re: LogicaL Chaos] * 1
    #28009915 - 10/21/22 08:10 PM (2 years, 2 months ago)

^^^^

ime those tomentose molds always have really disorganized & weird growth when compared with tomentose myc & once you see the mold vs tomentose myc in person or side by side it gets a bit easier to tell


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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OfflineLogicaL ChaosM
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Re: Tried Agar - anything save-able or should I toss? [Re: hazyhorse] * 1
    #28010013 - 10/21/22 09:58 PM (2 years, 2 months ago)

Definitely! Molds have some strange looking growth, randomness.

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Offlinehazyhorse
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Re: Tried Agar - anything save-able or should I toss? [Re: LogicaL Chaos] * 1
    #28010028 - 10/21/22 10:06 PM (2 years, 2 months ago)

randomness is a great way to put it!! when those molds grow out it just looks… bad. even myc that looks bad has some sort of organization to it. i had some mold on a plate (ended up turning purple at sporulation) & the earlier white growth provoked a visceral reaction in me, it was so gross lol


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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