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hazyhorse
scoobin



Registered: 03/19/19
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i love glass petris & you can too!! 28
#28006384 - 10/19/22 12:09 PM (1 year, 3 months ago) |
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glass petris:
actually kinda cool      been doing glass petris for a bit & figured a little write up on them might be nice to have for people considering using them. a lot of the info on glass petris i've found is scattered across random shroomery posts & not necessarily compiled in a way accessible to people unfamiliar with how to navigate the site. this post is more so just a bit of a guide for people who may be new to agar or who are looking to upgrade from more traditional no pour containers. there isn’t really anything groundbreaking here, so i doubt more experienced cultivators will get much useful information out of this. but there are a lot of ways for a cat to have skin & i feel like it never hurts to have a write up. pros & cons:pros:
- great visibility - really just crystal clear. a substantial upgrade over every other no pour container imo.
- reusable - being able to PC over & over saves you money each time you use the plates while also throwing out way less plastic. plus, you're not dependent on purchasing & waiting for petris to ship when you need new plates, they're always on hand.
- ability to no pour - i only tried pouring my glass dishes once, but it was enough of a pain in the ass that i decided to go back to no pour immediately. maybe i'm just kind of a baby with pouring, but i don't trust my pouring technique & the way i see it... you already have to PC the agar anyways & have to sterilize the glass plates too, doing a no pour method allows you to sterilize both agar & plates simultaneously & eliminates having to pour all together, two birds & all that. they've never boiled over on me, just don't fill them up to the brim with agar & you should be good

- feel & aesthetics - a subjective one for sure, but i personally think they feel great to handle & look amazing. good weight, feels sturdy in your hand. i'm a sucker for glass in general & the petris make me feel like some victorian biologist.
cons:- up front cost - i got mine for about $25 for a 10 pack. if you want a brand like pyrex, it’s likely going to be much more. if you’re trying to buy like 50, it is pretty expensive all at once. but every time you use them, you save money that you would have spent on plastic petris. over time what you spent cancels out. just order a set or two at a time if you like them.
- breakable - they can take a slight bit of abuse, but you don’t want to drop them or knock them into things. they are glass, they are fragile. i cracked one once just by putting a little too much pressure on the center of the lid trying to rip the seran wrap off the plate instead of unwrapping it. usually they are sturdy enough. as long as you treat them gently & are cognizant of how you handle & store them you should be completely fine. true boro/pyrex dishes will probably be very sturdy, but come at a higher cost
- slippery - i don’t find this to be a huge problem but i’ve seen people who have tried glass petris say they are too slippery or slide around a bunch. if you place the piece of seran wrap you took off the plate right underneath it, the plate shouldn't slide around on your surface while you're cutting. you can also just pick them up (carefully). i don't personally find them that challenging to work with
- cleaning - probably the biggest con of glass plates for most people. i won't lie, it kinda sucks to have to wash 40 plates all at once. i don't have to make agar too often when i do large batches, but if you were really burning through plates i could see this getting annoying. i've found that cleaning plates as they are done being used & storing them away until i need them again makes it much more manageable. it's way less work to clean 1 to 5 plates here & there than it is to wash your entire inventory of petris at once. as long as i'm not being lazy & cleaning my plates in a timely manner, they don't pile up & it's much less overwhelming when i do need to make more agar. i wash them & then dry with a microfiber cloth, but if you soak them in a vinegar + water solution you should be able to let them air dry
- may arrive broken - shipping glass shit is tough. you may end up with some broken plates. if you get in contact with the seller, they should replace them. still annoying, though
- potential con for SAB users - large batches can be annoying unless you have a huge SAB. i was using a 110qt & the 40+ plates in there were a bitch to unwrap & let dry. if you have a nice, big SAB i could see it being easier to do a large batch. it may be best to stagger your agar runs & make smaller batches a little more frequently if you find the large amount of plates unruly in the SAB. unwrapping the plates is a pretty slow process too, since you're constantly stirring up air when you unwrap the plates. it's doable but kind of slow, especially in those large batches. the flowhood is all around much better for them in this regard. still very doable in the SAB though!
sizes i use these plates specifically, but anything glass in the same sizes should be fine. there are a few sizes to choose from. i have the 60mm, 75mm, & 90mm plates. i mainly use the 75mm plates. i didn't realize it when i first bought them, but it seems most people use 90-100mm plates around here. personally, i think the 75mm is a great size to work with. the plates are big enough that you can let a culture grow out substantially but won't be pouring tons of agar that will go to waste when taking quick transfers. the 90mm plates feel a bit excessive to me, but are my second choice in size. i'll sometimes use them as spore germination plates or when growing out a culture to send to like 5+ grain jars, but recently i have been using them for water agar since the myc seemingly likes to run on WA. the 60mm are rather small, & i don't find myself using them much. they are good for quick transfers, but i don't know if they are worth buying specifically for that. the 75mm are the best balance of size to practicality imo. my plates are mostly 15mm deep, with the 90mm plates being 20mm deep. 15mm is plenty deep for no pour agar, just don't fill them up half way (this is way too much agar anyways) & don't throw around the PC while they are cooling & you should be primo  how to make themagain, nothing ground breaking, it's just a no pour agar tek. but i want this to make sense for people unfamiliar with agar & there are a few little details that might make glass petris easier to work with. if you really wanna pour them, you can follow sandman420's guide here to sterilize the plates. i recommend using them as no pour containers though. you can really use whatever agar recipe floats your boat. i personally use d3’s recipe but the recipe isn’t really what’s important here. pastywhyte's, or really any agar recipe will work. my process is essentially the same as making any no pour agar plates, just with some special considerations in method for the glass petris you can kinda try to figure out how much you'll need per plate... but most recipes are made relative to 90-100mm plates, & my dumb ass can never figure out the right ratios to get the exact amount of agar i'll need to fill the specific number of plates i'm making. to get around this, i've just been making more agar than i actually need & pouring the left over stuff into a PC safe vessel (a mason jar works excellently, just make sure the jar or vessel is able to vent properly during the cycle). PC this with your plates & you'll have agar ready for the next time you have to pour some.   (left over agar comes in very clutch when you just need to whip up a smaller batch of plates real quick. i've let jars sit unused for a month + & the agar is totally fine. you can reheat it by microwaving it for a few seconds at a time until it starts to melt. WATCH THE AGAR THE ENTIRE TIME IT'S IN THE MICROWAVE!! the moment you look away is the moment it will boil over. it happens fast, so the moment you see bubbling, stop the microwave. stir it up, & if there are still chunks, keep heating it until there aren't. alternatively, you could probably try scooping out chunks of agar into a pot & heat it that way, or just heat the jar while holding it with tongs in a pot of boiling water, or just place chunks of agar in the plates to melt in the PC cycle, or... anything idc get creative- it's your life) i use a pyrex liquid measuring cup to pour my agar into my plates, but you can pour out of the pot or out of a jar or whatever you want really, the little spout just makes it easier to pour without drips. i like to have a paper towel ready in my other hand to wipe up any agar drips off the vessel i'm pouring from like a sommelier pouring wine. i will let them solidify & cool off a little before placing the lid on them. 
now you'll wrap them in foil & stack them as high as you can in your PC. the way i lift my trivet & the size of my plates lets me do 7 plates in a stack in my 16qt, you may be able to do more or less depending on your set up. test this out before you wrap them, obviously. i like foil because it keeps the plates sturdy so they won't slide off each other during the cycle, & you can reuse the foil if you're careful enough! recently, i haven't been wrapping mine for the most part, as i use containers to PC them in now. but when i do have to wrap in foil, i find wrapping with two pieces & making a top & bottom like pictured makes it much easier to unwrap once you're ready to use them.  these are some quart sized ziplocks that fit my 75mm plates well. there is unfortunately some headspace lost to a slight taper, which leaves me able to do 6 plates at a time when i probably could fit 8 in there otherwise, but it's not a big enough issue for me to work around it yet. happened to have these on hand & wasn't using them so said fuck it lol. 24 plates a run is super acceptable for me right now, easy enough to make more containers if i needed them. i just drilled out some largish holes with a knife & covered them with 2 layers of micropore tape. i made about 6 holes, may bump it to 8 just to help with drying times, they can stay moist for quite a few days it seems. i cover the tops with foil in the PC just to keep the top tape from getting too soaked. you could probably used SFDs if you really wanted, but i don't see MP tape causing problems in this instance. i also like these containers because i can just slap the cover back on after i grab the plates i need, eliminating the need to wrap any unused plates if i don't want to. mainly it's just nice for going directly onto a shelf until needed- no need to set up the SAB or let them dry in front of a hood. i'm sure you could find plenty of containers that would work if you get creative! i also added a thin strip of foil under the bottom petri that i fold up the sides of the dishes so i can pick the whole stack up by just pinching the sides of the foil, just makes that bottom plate way easier to handle. (there are petri racks like this that can be used, but they are pretty pricey & will probably be too tall for 16qt cookers anyways. would probably be nice if it fits yours, though!) also forgot a pic here so... small batch!some people may prefer to wrap them with a single piece of foil, which SirPsycho outlines in this post.throw it all in your PC & run it for 20-60 minutes under pressure. you can easily fit 30+ plates in the 16qt cooker with foil or good containers. i usually do 30-45min for my agar. (if you don't know how to use a pressure cooker, check this out.) once it's done, carefully move the PC to a place to a place to cool. make sure to lift it without sloshing it around, as the agar is still hot in the plates & you don't want it spilling out. i let mine cool overnight in the PC. make sure your PC sits level, otherwise your plates may be a little slanted. not the biggest deal typically, but it may bother some people from an aesthetic stand point. you can check if your PC sits level by putting a jar in the PC & checking it with a bubble level set on top. if it's not level, you may have to set a towel under certain parts of your PC so that it sits level & your agar cools flat in the plates. you CAN take the plates out hot using jar grabbers or something, but you have to be really careful when moving the PC and plates because of the liquid agar. ime it's more worth it to just let them cool in the PC once it's cool enough that the agar has solidified, take the PC over to your SAB or flowhood & place the stacked plates in there. slowly begin to unwrap the plates. the foil gets kinda brittle after the PC & when it's all just once piece the plates can be tricky to get out of the foil without destroying it. the two pieces makes this a little easier. it's still kinda tricky, but you can keep the foil mostly intact if you're careful while unwrapping. i can reuse foil quite a few times as long as i don't have any major tears. just take it slow when unwrapping the plates- you don't wanna bump anything during this process. (if in an SAB, take your time. when i used an SAB with these petris, i just exposed the plates but didn't take them off the foil right away & let them dry for a bit. then, i'd remove them from the foil & let them dry more from there. again, smaller batches or a larger SAB makes this much easier.) once the plates are exposed, i'll grab them in stacks of 5 or so (grab as many as you feel comfortable with- better safe than sorry!) & place them where i want them to dry. i'll dry them in stacks of 10, but if you have a cramped work space you probably don't want giant stacks of breakable things where they can be easily knocked over. use your discretion with this. i'd leave the plates at least overnight in the SAB, a day or two if i could afford it (cover the arm holes). a few hours in front of a flowhood should help quite a bit, just make sure it doesn't go too long & dry the plates out. a lot of the condensation should clear up in storage or in use, but even if it doesn't don't worry too much. i find it generally clears up enough during use & shouldn't affect anything on the plates in my experience, you can leave the petris wrapped in foil for weeks on the shelf with no issue. they didn't seem to dry out as well this way, though, so it might not be the best for avoiding condensation. YMMV there, but they shouldn't contaminate on you. sir P leaves his PP5s completely unwrapped, & it seems that even my glass petris do OK unwrapped as long as they are handled delicately. however, i'm too clumsy to trust myself handling them unwrapped so i prefer to wrap them even when unused  when your petris are dry on the outside, they're ready to be wrapped!(in case you’re a dumbass like i am when i first got these, you need to wrap petris with cling wrap or parafilm… lol). i will use some plates fresh if i need to make any transfers, then will start wrapping in stacks. wrap your individual plates with a 1-2" wide roll of cling wrap once or twice around the bottom, making sure the cling wrap completely covers all gaps in the petri lid. when doing batch wrapping, it can be kinda hard to wrap tall stacks. cutting your cling wrap down to a size that can wrap 5 plates (or however many you are comfortable handling) will make this a lot easier than trying to wrap 10 all in one stack. i'll kind of pinch the cling wrap to the bottom plate with my pinky, then kind of lean them into the cling wrap & into my hand as i roll & continue to wrap. hard to explain, but it gets easier when you try it. alternatively, you can (very carefully!) wrap your plates like p9hu7 does in this handy gif:
 put your wrapped stacks & plates on whatever you use to hold plates  when you need to use plates, either unwrap the stack completely & individually wrap any plates you didn't use or just use a scalpel/your hands & cut out how many plates you want to use. [
i love mine, but not everyone is a fan.  if you’re unsure, order a 5 or 10 pack to test out before you order 60 of them & find out you hate them. practice how they feel to use in a realistic scenario before you make your first batch with agar in them. they may not be your thing, & that's totally fine, but i don't think they are as bad as i've seen people make them out to be sometimes. if you want to stick to reusable or no pour containers, glass petris are 100% worth the investment in my opinion. peace       
Edited by hazyhorse (10/27/23 08:41 PM)
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DERRAYLD
Constructus


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Re: i love glass petris & you can too!! [Re: hazyhorse] 3
#28018656 - 10/27/22 03:46 AM (1 year, 3 months ago) |
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Excellent write up, saves me the trouble  Exactly how I make my no pour glass petris.
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hazyhorse
scoobin



Registered: 03/19/19
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Re: i love glass petris & you can too!! [Re: DERRAYLD] 2
#28018658 - 10/27/22 03:49 AM (1 year, 3 months ago) |
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thank you friend! glad i could save ya the time. let the thread know if you have any personal tips i missed out on. they're pretty simple conceptually but not everyone has the background in agar & i wanted to lay it out as clearly as possible
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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UkaUka
Cpt



Registered: 05/12/22
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Loc: Eastern Europe
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Re: i love glass petris & you can too!! [Re: hazyhorse] 3
#28018662 - 10/27/22 04:00 AM (1 year, 3 months ago) |
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Thanks a lot mate! Just finished my first pour of agar, in 20 plastic and some glass dishes I got just for testing. The ability to do no pour in them is a he changer plus you don't end up with all this plastic waste. Keep it up!
-------------------- Writing is good, thinking is better. Cleverness is good, patience is better. looking for any give-away spore prints.
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Shrimps
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Re: i love glass petris & you can too!! [Re: hazyhorse] 2
#28018663 - 10/27/22 04:00 AM (1 year, 3 months ago) |
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Very nice write up Hazy!
I am also using Glass petris since I started. It was more because I could get them for free at that time, and I have never regret getting those.
Although depending on which Glass they're made of, when one of mine break it always hurts
I totally agree with you, Glass is worth the investment. And I am not a fan of wasting that much plastic either ^^
mush love <3
edit: and they feel much nicer than plastic imo
Edited by Shrimps (10/27/22 04:05 AM)
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hazyhorse
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Re: i love glass petris & you can too!! [Re: UkaUka] 2
#28018668 - 10/27/22 04:08 AM (1 year, 3 months ago) |
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glad you enjoyed the read uka! yeah, the ability to no pour these is great. it's a drop in the bucket at the end of the day, but it does make me feel a little better personally to not be throwing out so many petris all the time.
shrimps, glad you're on the glass petri wave too <3 it does hurt my soul whenever i break a petri, but luckily it doesn't happen too often. i'm surprised how sturdy they are sometimes. mush love friend!! also, excellent avatar
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
Edited by hazyhorse (10/27/22 04:21 AM)
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SirPsycho
Purple Belt in Google-Fu



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Re: i love glass petris & you can too!! [Re: hazyhorse] 2
#28018683 - 10/27/22 04:41 AM (1 year, 3 months ago) |
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What would happen if I left them in the foil until use? One of the main reasons I chose no-pour to start with is not having to set up the SAB timed to the PC cycle and then leaving it out while they cool.
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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smokypi
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Re: i love glass petris & you can too!! [Re: hazyhorse] 3
#28018686 - 10/27/22 04:48 AM (1 year, 3 months ago) |
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Thank you for the write up! I was doing agar with different kinds of pp5 containers, but was never really satisfied with handling and visibility. I'm going to order some glass petris now, found a good deal online in my country.
-------------------- “I am in fact, a hobbit in all but size” ------------------------------ My first try at the SFF Shoebox TEK ------------------------------ PF TEK SFF Shoebox TEK
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Rusty2096
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Re: i love glass petris & you can too!! [Re: hazyhorse] 2
#28018696 - 10/27/22 05:00 AM (1 year, 3 months ago) |
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Excellent work. When I finally move to glass, I'll know where to start all thanks to you!
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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Suckatshrooms
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Registered: 09/26/22
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Re: i love glass petris & you can too!! [Re: Rusty2096] 2
#28018807 - 10/27/22 07:38 AM (1 year, 2 months ago) |
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I think it’s important to note that your current observations on glass being more fragile may just be brand related. I have Pyrex and noticed the opposite. They are way tougher and less fragile than the plastic. I’ve broken plenty of plastic on accident just trying to wrap, but no issue with the glass.
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Suckatshrooms
Seriously, don’t listen to me.


Registered: 09/26/22
Posts: 157
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Re: i love glass petris & you can too!! [Re: SirPsycho] 2
#28018813 - 10/27/22 07:42 AM (1 year, 2 months ago) |
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Quote:
SirPsycho said: What would happen if I left them in the foil until use? One of the main reasons I chose no-pour to start with is not having to set up the SAB timed to the PC cycle and then leaving it out while they cool.
That’s what I do. They come out of the foil in the SAB right before I use them.
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SirPsycho
Purple Belt in Google-Fu



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Re: i love glass petris & you can too!! [Re: Suckatshrooms] 2
#28018814 - 10/27/22 07:43 AM (1 year, 2 months ago) |
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Quote:
Suckatshrooms said:
Quote:
SirPsycho said: What would happen if I left them in the foil until use? One of the main reasons I chose no-pour to start with is not having to set up the SAB timed to the PC cycle and then leaving it out while they cool.
That’s what I do. They come out of the foil in the SAB right before I use them.
And just to double check, you let them sit in the foil for extended periods of time. I.E. days
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
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ruawakeyet


Registered: 05/03/21
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Re: i love glass petris & you can too!! [Re: SirPsycho] 2
#28018997 - 10/27/22 09:37 AM (1 year, 2 months ago) |
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Thank you hazyhorse! This write up is EXACTLY what I needed! I looked at and liked many options that people are using, but if I'm going to buy something new for my hobby, I'm going to get really picky about every little detail. I don't want to realize later on that I chose the wrong path again. (Like using jars for so long, only to realize they are the reason I get pissed off trying to use them.) Every other option had something about it I didn't like, but I think I can live with the few disadvantages these carry.
I also hadn't considered using different sizes for different purposes. I like that concept, even though I will probably go with one size to start. (Since I use A2G for my primary inoculation method, I'll probably go with the 90 mm, even though I could see ordering some 75s in the future for quick transfers like you mentioned.)
Ultimately for me, it comes down to not wanting to throw away plastic, wanting the best visibility I can get, and not having high walls to work around.
The gloves I wear for my SAB work are dish washing gloves, and they have a textured surface that should help grip the lid while using. I hope that will minimize the slick nature of these.
As far as them being breakable glass, I'm used to handling borosilicate functional glass. I have worked in a head shop before, and had to handle / clean glass that was much more expensive than these. That experience, in addition to what I own and use on the regular, gives me confidence that I can be careful enough with these that they will last for a long time.
But if I end up not liking these, I'm going to blame you hazyhorse! (JK!)
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SirPsycho
Purple Belt in Google-Fu



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Re: i love glass petris & you can too!! [Re: ruawakeyet] 3
#28019193 - 10/27/22 11:20 AM (1 year, 2 months ago) |
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Quote:
ruawakeyet said:

Thank you hazyhorse! This write up is EXACTLY what I needed! I looked at and liked many options that people are using, but if I'm going to buy something new for my hobby, I'm going to get really picky about every little detail. I don't want to realize later on that I chose the wrong path again. (Like using jars for so long, only to realize they are the reason I get pissed off trying to use them.) Every other option had something about it I didn't like, but I think I can live with the few disadvantages these carry.
I also hadn't considered using different sizes for different purposes. I like that concept, even though I will probably go with one size to start. (Since I use A2G for my primary inoculation method, I'll probably go with the 90 mm, even though I could see ordering some 75s in the future for quick transfers like you mentioned.)
Ultimately for me, it comes down to not wanting to throw away plastic, wanting the best visibility I can get, and not having high walls to work around.
The gloves I wear for my SAB work are dish washing gloves, and they have a textured surface that should help grip the lid while using. I hope that will minimize the slick nature of these.
As far as them being breakable glass, I'm used to handling borosilicate functional glass. I have worked in a head shop before, and had to handle / clean glass that was much more expensive than these. That experience, in addition to what I own and use on the regular, gives me confidence that I can be careful enough with these that they will last for a long time.
But if I end up not liking these, I'm going to blame you hazyhorse! (JK!)
Ditch the gloves, come over to the dark side. We got mushrooms.
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: i love glass petris & you can too!! [Re: SirPsycho] 1
#28019329 - 10/27/22 12:38 PM (1 year, 2 months ago) |
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Quote:
SirPsycho said: Ditch the gloves, come over to the dark side. We got mushrooms.
I was afraid I was the reason you started that poll! 
I've got mushies too, they're just not fresh. I'm excited to freshen up my stash though!!!
Mush love!
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SirPsycho
Purple Belt in Google-Fu



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Re: i love glass petris & you can too!! [Re: ruawakeyet] 2
#28019335 - 10/27/22 12:40 PM (1 year, 2 months ago) |
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Quote:
ruawakeyet said:
Quote:
SirPsycho said: Ditch the gloves, come over to the dark side. We got mushrooms.
I was afraid I was the reason you started that poll! 
I've got mushies too, they're just not fresh. I'm excited to freshen up my stash though!!!
Mush love!
Been wanting to ask that for a while, you just reminded me.
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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hazyhorse
scoobin



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Re: i love glass petris & you can too!! [Re: ruawakeyet] 1
#28019349 - 10/27/22 12:44 PM (1 year, 2 months ago) |
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thank you all for the kind replies <3
Quote:
Suckatshrooms said: I think it’s important to note that your current observations on glass being more fragile may just be brand related. I have Pyrex and noticed the opposite. They are way tougher and less fragile than the plastic. I’ve broken plenty of plastic on accident just trying to wrap, but no issue with the glass.
yeah, i could see the pyrex ones being fucking sick. they are just like $100 for a pack & i found that hard to justify at my current income, but i think you're right about the brand. a quality borosilicate glass plate is gonna be borderline indestructible Quote:
SirPsycho said:
Quote:
Suckatshrooms said:
Quote:
SirPsycho said: What would happen if I left them in the foil until use? One of the main reasons I chose no-pour to start with is not having to set up the SAB timed to the PC cycle and then leaving it out while they cool.
That’s what I do. They come out of the foil in the SAB right before I use them.
And just to double check, you let them sit in the foil for extended periods of time. I.E. days
i never even considered this, but yeah if they were wrapped well enough i could see this being fine. i usually use janky foil with some holes in parts of it so it never really occurred to me lol. would be nice to validate that they can sit on the shelf for extended periods though. may add that consideration into the OP & do a little test myself next time i make plates. only caveat i could see is that the foil might not let them dry out so there may be condensation? but like i said, i always have it clear up once actually in use or in seran wrap. i get not wanting to set up the SAB for all this, though, so i hope the foil storage works well.
Quote:
ruawakeyet said:

Thank you hazyhorse! This write up is EXACTLY what I needed! I looked at and liked many options that people are using, but if I'm going to buy something new for my hobby, I'm going to get really picky about every little detail. I don't want to realize later on that I chose the wrong path again. (Like using jars for so long, only to realize they are the reason I get pissed off trying to use them.) Every other option had something about it I didn't like, but I think I can live with the few disadvantages these carry.
I also hadn't considered using different sizes for different purposes. I like that concept, even though I will probably go with one size to start. (Since I use A2G for my primary inoculation method, I'll probably go with the 90 mm, even though I could see ordering some 75s in the future for quick transfers like you mentioned.)
Ultimately for me, it comes down to not wanting to throw away plastic, wanting the best visibility I can get, and not having high walls to work around.
The gloves I wear for my SAB work are dish washing gloves, and they have a textured surface that should help grip the lid while using. I hope that will minimize the slick nature of these.
As far as them being breakable glass, I'm used to handling borosilicate functional glass. I have worked in a head shop before, and had to handle / clean glass that was much more expensive than these. That experience, in addition to what I own and use on the regular, gives me confidence that I can be careful enough with these that they will last for a long time.
But if I end up not liking these, I'm going to blame you hazyhorse! (JK!)
i'm very glad you found it helpful! if it helps even one person then writing this was worth it. everything is a give & take, all methods are gonna have their pros & cons. glass petris have a good balance imo. it's really important to have equipment & methods you like using. i realized my jank SAB was making me drag my feet on a lot of my sterile work because every time i used it i hated it more & more lol.
the visibility & reusable nature of them while also being no pour is the perfect combo for me. hands down the best no pour containers.
like sirpsycho seems to, i actually work barehanded! but if your gloves have good grip that's a huge plus. if you're used to handling glass to that degree, i'm sure you can handle some plates with a deft touch.
i think you'll like em, but i'll take the heat if you don't
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SirPsycho
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Re: i love glass petris & you can too!! [Re: hazyhorse] 2
#28019360 - 10/27/22 12:49 PM (1 year, 2 months ago) |
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Quote:
hazyhorse said:
like sirpsycho seems to, i actually work barehanded!
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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Re: i love glass petris & you can too!! [Re: SirPsycho] 3
#28019379 - 10/27/22 12:59 PM (1 year, 2 months ago) |
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raw dogging that shit just feels sooooo good
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bongoman
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28020551 - 10/27/22 11:36 PM (1 year, 2 months ago) |
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Thoughts on the height of glass petris?
I see they come in various heights: 15mm, 20mm, 25mm.
I imagine if doing no pour, the height might be a relevant issue?
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hazyhorse
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Re: i love glass petris & you can too!! [Re: bongoman] 2
#28020564 - 10/27/22 11:50 PM (1 year, 2 months ago) |
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ooh, good question bongo!
my 75mm plates are 15mm deep, the 90mm plates are 20mm deep. the 15mm deep plates handle no pour just fine. just don't overfill them. i'll add that consideration to the OP
edit: changed OP & added a link to the specific plates i use as well
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Edited by hazyhorse (10/28/22 12:13 AM)
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bongoman
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28020582 - 10/28/22 12:34 AM (1 year, 2 months ago) |
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That's great to know.
I was given 10 x 100mm x 15mm glass (borosilicate) petris by the person I bought my second hand flow hood from. One of my pet hates is throwing out plastic petris so I reckon it's time to dig out the glass stash and run a batch of 10 this weekend.
Maybe I'll invest in some smaller ones — it makes a lot of sense to keep the large ones for expanding clean transfers to go to grain and the smaller ones for quick transfers away from potentially dirty cultures.
You get much condensation with no pour? Maybe I need to go back and re-read the whole thread. Thanks for posting this!
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hazyhorse
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Re: i love glass petris & you can too!! [Re: bongoman] 1
#28020601 - 10/28/22 12:54 AM (1 year, 2 months ago) |
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oh fuck yeah! i recently got a flowhood & the glass petris are kick ass for it. i hated pouring them but they are very nice for no pour imo
i said this up in the OP too but the 75mm are great workhorse plates for stuff you’re not trying to grow out all the way. i also put the 75mm plates to grain & rarely use my 90mm but if you’re used to 90mm or just like they larger plate they are excellent to send to grain
i don’t get any bad condensation & the condensation that is there clears up after being on the shelf for a few days. i dry mine for a few hours in front of the flowhood, would leave them for a day in the SAB. if there is a big temp swing or something i’ll get slight condensation on plates but it’s easily remedied & that happens with all types of agar containers
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bongoman
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28020638 - 10/28/22 01:45 AM (1 year, 2 months ago) |
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So leaving them unwrapped in front of the flow hood but lids on?
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hazyhorse
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Re: i love glass petris & you can too!! [Re: bongoman] 1
#28021060 - 10/28/22 10:27 AM (1 year, 2 months ago) |
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yup! no real reason to take the lids off to dry them ime
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Suckatshrooms
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Re: i love glass petris & you can too!! [Re: SirPsycho] 1
#28021497 - 10/28/22 02:09 PM (1 year, 2 months ago) |
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Quote:
SirPsycho said:
Quote:
Suckatshrooms said:
Quote:
SirPsycho said: What would happen if I left them in the foil until use? One of the main reasons I chose no-pour to start with is not having to set up the SAB timed to the PC cycle and then leaving it out while they cool.
That’s what I do. They come out of the foil in the SAB right before I use them.
And just to double check, you let them sit in the foil for extended periods of time. I.E. days
I make a bunch at once and save them for weeks. Pour the plates, let solidify, wrap stacks of 4 in foil, PC at 1bar for 20min, let cool to room temp overnight, put in zip lock bag until needed.
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bongoman
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Re: i love glass petris & you can too!! [Re: Suckatshrooms] 1
#28026448 - 10/31/22 04:08 PM (1 year, 2 months ago) |
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And when you wrap them, are you using parafilm or grafting tape?
I'm finding the extra weight of the base and the lid puts more stress on the wrap — I find I need to be a bit more careful handling them so that the wrap doesn't work loose.
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hazyhorse
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Re: i love glass petris & you can too!! [Re: bongoman] 1
#28026496 - 10/31/22 04:38 PM (1 year, 2 months ago) |
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i personally use a 1”-2” wide roll of cling wrap, wrapped around twice typically. you do need to be a bit more conscientious when wrapping the glass petris. the wider rolls of cling wrap work wonderfully for me
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bongoman
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Re: i love glass petris & you can too!! [Re: hazyhorse] 3
#28027379 - 11/01/22 04:32 AM (1 year, 2 months ago) |
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Look what arrived today: 36 x 75mm x15mm borosilicate plates:
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Shrimps
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Re: i love glass petris & you can too!! [Re: bongoman] 1
#28027390 - 11/01/22 04:58 AM (1 year, 2 months ago) |
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Quote:
bongoman said: Look what arrived today: 36 x 75mm x15mm borosilicate plates:


You are going to have much joy with them
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smokypi
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Re: i love glass petris & you can too!! [Re: bongoman] 1
#28027491 - 11/01/22 07:28 AM (1 year, 2 months ago) |
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Quote:
bongoman said: Look what arrived today: 36 x 75mm x15mm borosilicate plates:

Nice! Tomorrow my order will arrive if the tracking is right. Have ordered 18x 80x15mm. Have fun!
-------------------- “I am in fact, a hobbit in all but size” ------------------------------ My first try at the SFF Shoebox TEK ------------------------------ PF TEK SFF Shoebox TEK
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Suckatshrooms
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Re: i love glass petris & you can too!! [Re: bongoman] 1
#28028407 - 11/01/22 06:28 PM (1 year, 2 months ago) |
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Quote:
bongoman said: And when you wrap them, are you using parafilm or grafting tape?
I'm finding the extra weight of the base and the lid puts more stress on the wrap — I find I need to be a bit more careful handling them so that the wrap doesn't work loose.
I use Parafilm. I haven’t had that problem, but once they’re wrapped, I don’t really handle them much so 🤷
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SpreadTheSpore
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28028943 - 11/02/22 04:29 AM (1 year, 2 months ago) |
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Excellent write up! Thank you for providing the information to us. Just stumbled upon these dishes, 10 each size, secondhand, traded for a bottle of wine! Seems like a good deal right?!
Gonne use your tek this weekend!
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SpreadTheSpore
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Re: i love glass petris & you can too!! [Re: Suckatshrooms] 1
#28028945 - 11/02/22 04:33 AM (1 year, 2 months ago) |
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Sounds like a nice addition.
To be sure: - You don’t do anything to the dishes to get them dry? Reading that they could be quite wet when taken uit of the PC - When you take them out of the PC, wrapped in aluminium foil. Do you put them in the ziplock with the aluminium foil still wrapped?
*not sure if I could ask these questions in this journal.. hope it is alright.
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Suckatshrooms
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Re: i love glass petris & you can too!! [Re: SpreadTheSpore] 1
#28029213 - 11/02/22 09:59 AM (1 year, 2 months ago) |
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Quote:
SpreadTheSpore said: Sounds like a nice addition.
To be sure: - You don’t do anything to the dishes to get them dry? Reading that they could be quite wet when taken uit of the PC - When you take them out of the PC, wrapped in aluminium foil. Do you put them in the ziplock with the aluminium foil still wrapped?
*not sure if I could ask these questions in this journal.. hope it is alright.
When the PC gets back to room temp, I do one of two things.
1) If they’re being stored for a long time, I take them out and straight into zip lock bags, foil and all and straight into the lab refrigerator at 5°C
2) If they’re being used soon, I just put them in my SAB.
They always have some condensation, but usually nothing major. I just ignore it and use as normal. After a week the condensation disappears on its own. Sometimes for unknown reasons, I’ll have one with a pool of water in the bottom. With these, I still just use as normal, but in the SAB, I just pour the excess water out right before I do my transfer or whatever. After I Parafilm wrap it, I usually sanitize the outside with IPA just because I get nervous about any nutrient water from pouring it out sticking to the side and letting bacteria migrate up past the Parafilm.
Pre pour has never made plates as immediately perfect as normal, but for the ease of so few steps, I love it.
Edited by Suckatshrooms (11/02/22 10:00 AM)
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Suckatshrooms
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Re: i love glass petris & you can too!! [Re: SpreadTheSpore] 3
#28029219 - 11/02/22 10:03 AM (1 year, 2 months ago) |
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Here’s a pic I just took this morning. It’s dated 22OCT, and you can see there’s still some condensation.
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hazyhorse
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Re: i love glass petris & you can too!! [Re: SpreadTheSpore] 1
#28029515 - 11/02/22 02:23 PM (1 year, 2 months ago) |
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Quote:
SpreadTheSpore said: Sounds like a nice addition.
To be sure: - You don’t do anything to the dishes to get them dry? Reading that they could be quite wet when taken uit of the PC - When you take them out of the PC, wrapped in aluminium foil. Do you put them in the ziplock with the aluminium foil still wrapped?
*not sure if I could ask these questions in this journal.. hope it is alright.
you can totally ask questions here! i wanted it to be an open forum for glass petri questions. otherwise i could have just made it so no one can reply 
i don’t like, hand dry the dishes but i do leave them in the SAB/flowhood for a bit so the outside dries off. cling wrap will slip off if the glass is wet. condensation almost always clears up on its own & tbh i think it’s something people worry about a bit too much. it should go away after a bit, or the temperature in the room is just different than the plates & you may get a bit regardless of the plate being plastic or glass or whatever
i just personally take them out of the foil in the hood/SAB & wrap them in stacks with seran wrap once dry. but you should be able to just put the foil wrapped plates straight into a ziplock bag (make sure it’s a fresh bag, as they come sterile out of the package). you could probably use an oven bag & sterilize that in the PC too if you wanted to reuse a bag
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Re: i love glass petris & you can too!! [Re: bongoman] 1
#28029516 - 11/02/22 02:24 PM (1 year, 2 months ago) |
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beautiful plates bongo! i hope you love em as much as i do (:
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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Shrimps
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28029605 - 11/02/22 03:22 PM (1 year, 2 months ago) |
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Regarding the wet Petris out of the PC. You can also heat up your oven to about 80C / 176F also put them for like a couple of minutes into the oven. I don't pre pour my dishes and dry them always in the oven. I don't see why that shouldn't work also with pre-poured dishes You can just turn the oven off and let them cool down in the oven.
As long as they stay in the aluminium foil wrapped, they stay contam-free.
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hazyhorse
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Re: i love glass petris & you can too!! [Re: Shrimps]
#28030170 - 11/02/22 08:28 PM (1 year, 2 months ago) |
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i would be worried the oven may dry out the agar pretty quick. i’ve seen people like josex swear contams can make their way through foil, but YMMV with that. i can neither confirm nor deny
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smokypi
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28030538 - 11/03/22 01:19 AM (1 year, 2 months ago) |
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New petris arrived yesterday! Used gw agar without colouring, let's see how i like them. They are currently drying up in my sab - doing some transfers later.
-------------------- “I am in fact, a hobbit in all but size” ------------------------------ My first try at the SFF Shoebox TEK ------------------------------ PF TEK SFF Shoebox TEK
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hazyhorse
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Re: i love glass petris & you can too!! [Re: smokypi] 1
#28031131 - 11/03/22 12:53 PM (1 year, 2 months ago) |
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good luck friend! i hope you enjoy them!
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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SingularFusion


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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28041327 - 11/08/22 10:57 PM (1 year, 2 months ago) |
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nice writeup Hazy
I still have some glass around, it's what I started with... I used to sterilize them in foil towers of six plates in the oven. Then pour them in the SAB like normal plates. That's why six to a stack, otherwise they get heavy to lift from the bottom up with a two finger grip. I was always scared the things would explode in my hand from the grip pressure
Anyway, I abandoned them eventually because I just needed so many it became a bit of a PITA. But for no pour and the way you do things I can definitely see the given advantages, especially the whole feeling like some sorta Victorian mycologist. For a noob, that was almost the best part of them 
It would be nice to not use single use PS. The last thing the world needs is more plastic waste. But the convenience of them is useful, when you have the planning skills of a goldfish, such as I do
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SirPsycho
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So I'm supposed to be getting some shrink wrap sleeves delivered today. Gonna try running some agar in them see if we can sterilize and wrap/seal in one go.
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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SingularFusion


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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28041707 - 11/09/22 08:26 AM (1 year, 2 months ago) |
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sounds interesting Sir
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hazyhorse
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Re: i love glass petris & you can too!! [Re: SirPsycho] 1
#28041866 - 11/09/22 10:19 AM (1 year, 2 months ago) |
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aye thanks nef! yeah, pouring them sucks ass lol. literally tried it once & was like “never again” lol. luckily they really rock as no pour containers. more delicate than your traditional PP5 no pour but i think the visibility makes it worth it.
ngl it still feels pretty satisfying to hold these plates with a beautiful culture on them lol. when i first got them it felt like such a huge upgrade. makes ya feel legit when you’ve been using plastic take out cups ya know? lol
i totally get why people use plastic petris tho, no judgement there & the amount of plastic saved by using glass petri dishes is realistically a drop in the bucket compared to most of the waste humans produce. but it’s something & i do like having them on hand vs having to wait on shipping if i were to run low for whatever reason
Quote:
SirPsycho said: So I'm supposed to be getting some shrink wrap sleeves delivered today. Gonna try running some agar in them see if we can sterilize and wrap/seal in one go.
fuck yeah, keep us updated sir P. i was thinking about trying to get them to stack safely in an oven bag or something to accomplish the same thing. foil + oven bag would definitely work, i just feel like they’ll topple over if it’s just bare glass plates stacked. the shrink wrap sounds like an excellent idea
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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SirPsycho
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Re: i love glass petris & you can too!! [Re: hazyhorse] 2
#28042587 - 11/09/22 06:31 PM (1 year, 2 months ago) |
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So I'm probably not gonna have time to get that agar done tonight, still working on getting the bottles going but here's a shot of some old plates in the sleeve for fit.

I can probably throw 4 more in there
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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hazyhorse
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28042664 - 11/09/22 07:28 PM (1 year, 2 months ago) |
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dude fuck yeah. at that point i think the height of your PC would be the limiting factor. im a pleb with a 16qt presto ;(
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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Rusty2096
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28042674 - 11/09/22 07:34 PM (1 year, 2 months ago) |
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Quote:
hazyhorse said: dude fuck yeah. at that point i think the height of your PC would be the limiting factor. im a pleb with a 16qt presto ;(
Bro, I'm a pleb with a 7qt presto PC. That said I bought a 23qt that should be here in 10 days or so
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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SirPsycho
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28042734 - 11/09/22 08:10 PM (1 year, 2 months ago) |
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Quote:
hazyhorse said: dude fuck yeah. at that point i think the height of your PC would be the limiting factor. im a pleb with a 16qt presto ;(
Lmfao, I got a 16 too. AA though. Thing is a beast.
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28042840 - 11/09/22 09:06 PM (1 year, 2 months ago) |
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Quote:
Rusty2096 said: Bro, I'm a pleb with a 7qt presto PC. That said I bought a 23qt that should be here in 10 days or so 
hahahaha i used to be in the same boat man, shit is rough. i was doing like 4 pint jars at a time, i could do a run of like 8 pasty plates. shit was workable but also sucked. i had roommates who didn’t know i grew at the time & so i had to run it on a hot plate in my closet lolol. the 23qt will change your cultivation life, not even kidding
Quote:
SirPsycho said: Lmfao, I got a 16 too. AA though. Thing is a beast.
ahh im jealous!! those AA cookers are such beautiful appliances. for the price the presto kicks ass, but the AA are just kick ass
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SirPsycho
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28044044 - 11/10/22 05:05 PM (1 year, 2 months ago) |
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 Aight let's see how this shrink wrap thing goes. The second stack is wrapped in parchment paper
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28044123 - 11/10/22 05:52 PM (1 year, 2 months ago) |
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hell yeah man, stoked to see the results
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SirPsycho
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28044127 - 11/10/22 05:56 PM (1 year, 2 months ago) |
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Quote:
hazyhorse said: hell yeah man, stoked to see the results
Me too, still debating opening the pc tonight or letting it cool overnight like I usually do.
6 mins left on the timer
--------------------
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Rusty2096
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28044129 - 11/10/22 05:57 PM (1 year, 2 months ago) |
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I vote open 
Edit: no so sure anymore might make a mess
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
Edited by Rusty2096 (11/10/22 05:57 PM)
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SirPsycho
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Re: i love glass petris & you can too!! [Re: Rusty2096] 2
#28044132 - 11/10/22 05:58 PM (1 year, 2 months ago) |
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Quote:
Rusty2096 said: I vote open 
Lmfao, I might just do it cause you asked and I love your new avatar.
That shit is
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28044161 - 11/10/22 06:12 PM (1 year, 2 months ago) |
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the only time i opened it without letting it fully cool it was a logistical nightmare of trying to not move the plates so much that i spill agar out of them lol
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28044231 - 11/10/22 06:44 PM (1 year, 2 months ago) |
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Alright then since we're all on the same page I'll see y'all in the morning. It's cooling now.
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho] 1
#28044246 - 11/10/22 06:51 PM (1 year, 2 months ago) |
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glad you didn’t let rusty get the best of you this time <3
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28044767 - 11/11/22 04:29 AM (1 year, 2 months ago) |
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Well, it didn't melt like cling wrap but it did end up tearing where it's circled. Gonna try another run this morning at some point and hopefully have updates by the evening.
--------------------
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28044824 - 11/11/22 06:04 AM (1 year, 2 months ago) |
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Is it expensive? Maybe you could multi layer it?
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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Re: i love glass petris & you can too!! [Re: Rusty2096]
#28044880 - 11/11/22 07:26 AM (1 year, 2 months ago) |
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Quote:
Rusty2096 said: Is it expensive? Maybe you could multi layer it?
15 cents a piece. Couldn't find any rolls on Amazon that I was confident was actually heat film.
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28045075 - 11/11/22 09:50 AM (1 year, 2 months ago) |
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So this time I left the bag whole. Last time I cut off the bottom to make it into an actual sleeve and figured I filled the sleeves too high.

Still tore.
Maybe if I get the 8 inch wide bags instead of 7.
--------------------
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28045200 - 11/11/22 11:23 AM (1 year, 2 months ago) |
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I guess an extra inch sounds about right to prevent tearing
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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Re: i love glass petris & you can too!! [Re: Rusty2096] 2
#28045216 - 11/11/22 11:36 AM (1 year, 2 months ago) |
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Fingers crossed.
In the meantime, I hit these bags I do have with a hair drier and they'll be perfect for wrapping plates when they go in the fridge for culture storage
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28045237 - 11/11/22 11:57 AM (1 year, 2 months ago) |
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As a matter of fact, I just re-wrapped my fridge plates. This will make it much easier to keep track of what I got put away.
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28045315 - 11/11/22 12:59 PM (1 year, 2 months ago) |
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ooh, nice. hopefully you can get them to withstand the PC cycle, but using them for culture storage seems super nice the way you have it set up
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28045321 - 11/11/22 01:02 PM (1 year, 2 months ago) |
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Quote:
hazyhorse said: ooh, nice. hopefully you can get them to withstand the PC cycle, but using them for culture storage seems super nice the way you have it set up
The 8x12 bags I just ordered had sizing instructions too and the 7 were about 1/4" too small according to the formula provided. Hopefully 8 isn't too big
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28045326 - 11/11/22 01:05 PM (1 year, 2 months ago) |
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it would be nice to be a tight stack, but as long as they aren't toppling over i'd call it a win.
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SirPsycho
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Re: i love glass petris & you can too!! [Re: Suckatshrooms]
#28052910 - 11/15/22 03:11 PM (1 year, 2 months ago) |
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Quote:
Suckatshrooms said:
Quote:
SirPsycho said: What would happen if I left them in the foil until use? One of the main reasons I chose no-pour to start with is not having to set up the SAB timed to the PC cycle and then leaving it out while they cool.
That’s what I do. They come out of the foil in the SAB right before I use them.
No update on the shrink wrap, still waiting on the bigger bags but just wanted to say I've done the leaving in the foil thing my last couple of runs.
Just let them cool overnight and then leave them wrapped in the tin foil until next time I got the SAB set up, at which point I wrap them like hazy showed p9 doing. Works great, all my plates last batch were clean and just wrapped the second run today.
Hell there's even less condensation than when I was taping them with micropore before the pc
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28053065 - 11/15/22 04:05 PM (1 year, 2 months ago) |
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thanks for following up, psycho. the foil is just so dead simple & i also find there’s very little condensation or moisture that way. glad they seem to store fine on the shelf in the foil too!!
i’ve been brainstorming cutting foil in a way to make unwrapping them a bit easier. the way i’m thinking probably wouldn’t be the best for long term storage but if you’re putting them immediately in the SAB or in the hood i think it would make the foil a breeze to peel off. im up skiing rn but if im not too busy later i’ll play around with it & potentially update
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28053077 - 11/15/22 04:09 PM (1 year, 2 months ago) |
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If you use the extra wide foil (25 inch) and you rip out a square you can set a stack of 8 or so plates right in the centre and have enough foil to completely fold over the top of the stack.
I'll take some step by step pics of how I do it next time.
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28053101 - 11/15/22 04:17 PM (1 year, 2 months ago) |
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fuck yeah, looking forward to it.
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28054400 - 11/16/22 12:19 PM (1 year, 2 months ago) |
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Here's how I wrap stacks in foil. I went a bit high this time, I find 8 of my plates is ideal. Make sure you hold everything down at the top of the stack as you fold it over. One hand holds the pieces on the top of the stack the other hand does the folding.
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28054542 - 11/16/22 02:09 PM (1 year, 2 months ago) |
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fuck yeah, thanks sirP! i’ll update the OP a bit later when i’m off work
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SirPsycho
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28054674 - 11/16/22 03:28 PM (1 year, 2 months ago) |
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Well the bigger shrink wrap bags are a bust too. Literally, they also bust open. The force of them shrinking also deformed a plate on each stack. Weirdly enough it was the 3rd plate from the top of each stack.
--------------------
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Rusty2096
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28055031 - 11/16/22 07:00 PM (1 year, 2 months ago) |
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Any chance to get thicker ones?
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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SirPsycho
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Re: i love glass petris & you can too!! [Re: Rusty2096] 2
#28055050 - 11/16/22 07:11 PM (1 year, 2 months ago) |
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Quote:
Rusty2096 said: Any chance to get thicker ones?
Don't think it'll help. It looks like it has to do with just shrinking the whole thing at once. Plus it already deformed my plates at this thickness.
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28055909 - 11/17/22 10:09 AM (1 year, 2 months ago) |
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bummer the shrink wrap didn’t work out, that would have been mad convenient. that’s super weird about them deforming the plates too lol really random. i appreciate ya giving it a go in the name of

at least the foil is pretty dead simple. hard to beat & if it works for long term storage, all the better
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SirPsycho
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28056196 - 11/17/22 01:32 PM (1 year, 2 months ago) |
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Quote:
hazyhorse said: bummer the shrink wrap didn’t work out, that would have been mad convenient. that’s super weird about them deforming the plates too lol really random. i appreciate ya giving it a go in the name of

at least the foil is pretty dead simple. hard to beat & if it works for long term storage, all the better
I guess the shrink wrap plastic is actually thougher than the polypropylene the plates are made out of. I imagine with your glass plates that would mean just larger tearing in the wrap.
Yeah, the foil seems to work just fine. Not sure about long term cause I've managed to find time to wrap with cling the next day so far but if I do happen to find an upper limit for how long they store in just foil I'll let you know.
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28056218 - 11/17/22 01:42 PM (1 year, 2 months ago) |
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yeah the glass plates would probably just destroy the wrap at that point, seems finicky. i wrap mine pretty immediately after they dry & see no reason not to, but i get that it would be pretty convenient to just toss the foil wraps on the shelf out of the PC. josex mentioned somewhere he swears pores can find their way through folded foil (why he doesn’t recommend storing syringes in foil long term) but yeah the upper limit would be cool to know for people who may wanna go that route
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SirPsycho
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28056274 - 11/17/22 02:16 PM (1 year, 2 months ago) |
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Yeah, I'm not too keen on trusting the foil completely myself.
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28056934 - 11/17/22 07:14 PM (1 year, 2 months ago) |
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me either, anything not truly sealed is pretty suspect to me. not hard to give em the ol’ p9 seran wrap after they dry imo
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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hazyhorse
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28059449 - 11/19/22 03:27 AM (1 year, 2 months ago) |
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updated the OP a little (added a link to ur post on wrapping, psycho!!)
also, a thought crossed my mind for potential long term storage right out of the PC. might be possible to put the foil wrapped plates in an oven bag before PCing & just leave them in the oven bag sealed on the shelf until you need to use them. i doubt it's something i would do often myself, but i might give it a go just to see if it works & give people the option if it's how they want to go about it.
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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LogicaL Chaos
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Re: i love glass petris & you can too!! (moved) [Re: hazyhorse] 2
#28059920 - 11/19/22 11:34 AM (1 year, 2 months ago) |
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This thread was moved from the user's journal.
Reason: Moved at OP's request.
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Shrimps
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Re: i love glass petris & you can too!! (moved) [Re: LogicaL Chaos] 2
#28059943 - 11/19/22 11:43 AM (1 year, 2 months ago) |
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Great decision! This need more attention! Just stocked up to 40 dishes 
Thinking about building a petridishholder for my incubation-box (just a box), to save up more space. They are actually easy to build (and not expensive to buy).
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hazyhorse
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Re: i love glass petris & you can too!! (moved) [Re: Shrimps] 2
#28059963 - 11/19/22 11:52 AM (1 year, 2 months ago) |
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thanks shrimps! it just occurred to me that it probably wasn’t being seen in searches since it was in my journal
i’d love to see that petri holder if you have any pics/sketches of what it would look like! right now i just store my empty dishes in a shoebox padded with old socks dishes with agar just get stacked in the corner of my cult shelf
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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Shrimps
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Re: i love glass petris & you can too!! (moved) [Re: hazyhorse] 3
#28059966 - 11/19/22 11:55 AM (1 year, 2 months ago) |
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Yeah when I got my stuff together, I will make some pic's how to build it. Will make updates then ;-)
Should look something like this (what I had in mind)
Edited by Shrimps (11/19/22 12:33 PM)
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B Traven
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Re: i love glass petris & you can too!! (moved) [Re: Shrimps] 2
#28060100 - 11/19/22 01:17 PM (1 year, 2 months ago) |
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Nice to see this discussion.
With all respect due to Roger Rabbit, I recall seeing him comment some years back that "glass sucks for mycology." That alone put me off of trying to do anything with a stash of old pyrex plates from a university lab that someone had given me.
I finally decided to give them a shot, and now I could never go back. (Except when I run out of glass petris, and run pastyplates to make up the difference)
I had no idea what I was doing, and still don't. That said, I'm pretty satisfied with my current results. My techniques don't quite align with the ones I'm reading about here. I stumbled blindly into no-pour glass petris, and approached them the same way I'd been doing pastyplates. I haven't yet found a compelling reason to change.
I start with my pot o hot agar, and pour it into the petri bottoms arrayed on the countertop. I then leave them, uncovered, until they cool completely.
Once cool, I put the covers on and individually wrap them in foil. They then get PC'ed in an instant pot for 1.5 hours.
I let the instant pot sit overnight, or sometimes much longer if I don't have a chance to get to it. I either pull the fully cooled plates from the instant pot and immediately work with them in my SAB, or I pull them from the instant pot and put them in my SAB for later work. That's usually when I want to free up the instant pot for another batch, and then do all my transfers at once on the third day.
Transfers are a crude process round my way. Unwrap however many plates the master plate will be donating to, and stack them right on top of the still-wrapped plates in the SAB. Let the air settle, then have at it. Repeat until done.
I don't wrap my plates in cling wrap, nor do I do any drying. Condensation has never really been an issue for me with my glass petris.
PC'ed plates never really sit around my place. I'm pretty much making them as I need them. The same holds true for plates that need to be washed, and plates that have just been washed. I'm usually running transfers to free up plates that I need to run more transfers. I kind of like having a limited supply, it keeps me sharp and organized. The dish and storage burden is so spread out that I barely notice it.
I keep wondering if I should start wrapping my plates with parafilm after transfers. I keep not getting any further than just sort of thinking about it.
-------------------- Beware of advice- even this.
Edited by B Traven (11/19/22 01:21 PM)
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hazyhorse
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Re: i love glass petris & you can too!! (moved) [Re: B Traven] 2
#28060150 - 11/19/22 01:58 PM (1 year, 2 months ago) |
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yeah, part of the reason i wanted to do this write up was because there was very little collective info on glass petris & a lot of the stuff i found by searching was just old posts hating on them lol. i fucking love mine & wanted to encourage people to try them out
when i say i dry my petris, i more mean dry the outside so that the cling wrap or parafilm doesn’t slip off. i don’t find condensation much of an issue with them either
you don’t see contam by not wrapping your plates? maybe the pyrex ones have a better seal, but i saw rampant contams pretty quick when i didn’t wrap mine lol. i learned that lesson really fast
@shrimps: that’s so sexy!! i love little diy projects, definitely a good way to keep them safe!! share pics if you get around to making your own, i’d love to see it (:
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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B Traven
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Re: i love glass petris & you can too!! (moved) [Re: hazyhorse] 1
#28060174 - 11/19/22 02:10 PM (1 year, 2 months ago) |
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Yeah, that's kind of what I figured.
The pyrex covers are pretty substantial, and I think the weight holds them in place pretty well.
I do see some contaminated plates, but not very many, and the nature of the contamination suggests to me that it's mostly due to my less than stellar transfer techniques.
-------------------- Beware of advice- even this.
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hazyhorse
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Re: i love glass petris & you can too!! (moved) [Re: B Traven]
#28060188 - 11/19/22 02:23 PM (1 year, 2 months ago) |
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i’d absolutely love to try out some pyrex plates, they look beautiful & i know the quality is top notch. they are just so pricey to buy /:
for sure, once you get a handle on things you can kinda infer where you’re getting contams from. a wrap isn’t gonna hurt anything but hey, if it ain’t broke…
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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Moopers
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Re: i love glass petris & you can too!! (moved) [Re: hazyhorse] 1
#28070653 - 11/26/22 07:16 PM (1 year, 2 months ago) |
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thanks for this guide!
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LotKid
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Re: i love glass petris & you can too!! (moved) [Re: Moopers] 3
#28070810 - 11/26/22 09:56 PM (1 year, 2 months ago) |
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Shrimps
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Re: i love glass petris & you can too!! (moved) [Re: LotKid]
#28070895 - 11/26/22 11:31 PM (1 year, 2 months ago) |
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Quote:
LotKid said:


I love glass too

Damn, love your FH! Are those autoclave containers for your dishes?
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LotKid
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Re: i love glass petris & you can too!! (moved) [Re: Shrimps] 2
#28071234 - 11/27/22 08:18 AM (1 year, 1 month ago) |
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Yes
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kirkeng
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Re: i love glass petris & you can too!! [Re: LotKid] 4
#28071295 - 11/27/22 09:24 AM (1 year, 1 month ago) |
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I’ve said it before but I’ll say it again in hopes of helping people get over the price tag of glass. Call your local university, many have reuse programs or recycle centers. They are not always available but when they are you can find glass petris for 25-50 cents a pop, at least at my local university. Sometimes the deal is better, I’ve gotten cases of hundreds for 10$. Highly recommend the effort. Quite frankly they often have a lot of highly discounted lab equipment. Many of these programs are open to the general public as well, I have never set foot in a university in my life but still reap those benefits from time to time.
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hazyhorse
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Re: i love glass petris & you can too!! [Re: kirkeng]
#28077211 - 11/30/22 09:51 PM (1 year, 1 month ago) |
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Quote:
LotKid said:


I love glass too

the corning dishes look so nice. would love to grab those or pyrex ones one day
they go so great with cling wrap, don't they? 
@moopers: no problem!! thanks for your natalensis thread, it's so incredibly helpful to have all that info compiled in one place!!
@kirkeng: that's an excellent thing to keep in mind. i knew they had old lab equipment & stuff for sale but never thought about the petri dishes. would be very awesome to pick up a bunch on the low!!
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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LotKid
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Re: i love glass petris & you can too!! [Re: hazyhorse] 3
#28079282 - 12/02/22 08:59 AM (1 year, 1 month ago) |
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Pyrex... corning... same thing... literally...
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hazyhorse
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Re: i love glass petris & you can too!! [Re: LotKid] 1
#28079487 - 12/02/22 12:03 PM (1 year, 1 month ago) |
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guess that makes it easy to choose
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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senatedestroyer
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28081035 - 12/03/22 09:24 AM (1 year, 1 month ago) |
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I’m so glad someone linked this post to my inquiry! I too scoured the internet and could find minimal info on pouring THEN sterilizing. I just pc’d mine last night and am patiently waiting to see if any contam shows on my control. My process was just about the same l. Wrapped in foil, individual paradigm today in my SAB. Any speed bumps you ran into to look out for?
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hazyhorse
scoobin



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aside from just handling them carefully & only wrapping/unwrapping in an SAB or in front of a flowhood, if the plates aren’t fully dry on the outside sometimes the wrap can slip off. that’s about the only thing i can think of
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Shrimps
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28081630 - 12/03/22 04:33 PM (1 year, 1 month ago) |
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Quote:
hazyhorse said: aside from just handling them carefully & only wrapping/unwrapping in an SAB or in front of a flowhood, if the plates aren’t fully dry on the outside sometimes the wrap can slip off. that’s about the only thing i can think of
That's why we use parafilm :3
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hazyhorse
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Re: i love glass petris & you can too!! [Re: Shrimps]
#28081644 - 12/03/22 04:41 PM (1 year, 1 month ago) |
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shhhhh i don’t wanna hear it shrimps!!!
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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sandman420
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Re: i love glass petris & you can too!! [Re: Anonymous] 2
#28081672 - 12/03/22 05:05 PM (1 year, 1 month ago) |
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Way cool write up! I have started using my glass plates for no pours after seeing you and Derayld having such good luck with them.
I'm such a dummy I always wondered how you wrapped them up with foil after putting the hot agar in...haha... I never even considered LETTING THEM COOL OFF.
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hazyhorse
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Re: i love glass petris & you can too!! [Re: sandman420] 2
#28082065 - 12/03/22 08:19 PM (1 year, 1 month ago) |
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yoo thank you sandman!! i hella appreciate it. they are such sick no pour containers & i'm shocked they don't seem to be more popular on here
if i had a dollar for every time i missed some really obvious shit in this hobby i'd be a rich man. i wondered the same thing when i first went to use them & had a big "no shit" moment when i realized i could just wait 5 minutes & then wrap them
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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tealseal
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28110065 - 12/23/22 03:18 PM (1 year, 1 month ago) |
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I just got 100 HG plates anf i regret not seeing this thread before i pulled the trigger on em. Ive always done no pour pasteys but wanted more viz, thought HG'S were the ticket but afyer the pc alot of them have this wierd film that wont come off. Even after a soak in vinegar and a iso bath. Plus some of then warped. I expected that after reading aliens HG plate thread. Gonna have to chalk that up to a lessoned learned and grab some glass.. I just hate the foil BS!! i wonder of theres a way to get around all the damn foil
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NuclearTidalWave
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Re: i love glass petris & you can too!! [Re: tealseal]
#28110091 - 12/23/22 04:02 PM (1 year, 1 month ago) |
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Quote:
tealseal said: I just got 100 HG plates anf i regret not seeing this thread before i pulled the trigger on em. Ive always done no pour pasteys but wanted more viz, thought HG'S were the ticket but afyer the pc alot of them have this wierd film that wont come off. Even after a soak in vinegar and a iso bath. Plus some of then warped. I expected that after reading aliens HG plate thread. Gonna have to chalk that up to a lessoned learned and grab some glass.. I just hate the foil BS!! i wonder of theres a way to get around all the damn foil
They make autoclaveable containers Like these
-------------------- Signature text goes here. NTW's LAGM 2.023 P̫̻̳̮͎̥̬͋͊̂͐Ä̡͓̜̩̤̠̮́́̔̽̇Ṛ̹̱ͅA̠͉͒F̹̦̣͇̘̻̍̎ͬ͋͒̅͌͘I̖̻ͨͧ̇̋ͯ͡L̩̩͋́M͕̳͎̱̎ ̤̜͇̰̉̿ͫ̑͑͡ͅC̶̲̝̉̔U͚̙͕̬̘̗̐ͮ̀ͣ̎L̏́̂͋Ṯ̥̪̠ͩ͛̃͊
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tealseal
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Comming in to save the day!!! Yeaa boii
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NuclearTidalWave
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I have not yet tried them but I've got some on the way. I also hate dealing with all the foil. FYI the ones I linked are only supposed to fit up 90mm plates. I've yet to find any that fit 100mm but also haven't looked that hard.
-------------------- Signature text goes here. NTW's LAGM 2.023 P̫̻̳̮͎̥̬͋͊̂͐Ä̡͓̜̩̤̠̮́́̔̽̇Ṛ̹̱ͅA̠͉͒F̹̦̣͇̘̻̍̎ͬ͋͒̅͌͘I̖̻ͨͧ̇̋ͯ͡L̩̩͋́M͕̳͎̱̎ ̤̜͇̰̉̿ͫ̑͑͡ͅC̶̲̝̉̔U͚̙͕̬̘̗̐ͮ̀ͣ̎L̏́̂͋Ṯ̥̪̠ͩ͛̃͊
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tealseal
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Looks like 9.5 inches.. I dont think that will fit in a 23Q presto Can anyone confirm?
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NuclearTidalWave
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Re: i love glass petris & you can too!! [Re: tealseal]
#28110109 - 12/23/22 04:11 PM (1 year, 1 month ago) |
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Sorry I kind of glossed over the fact you are aiming for no pours. I do poured and I sterilize my glass plates in the oven so size isn't really an issue. I should be getting mine in a few days though and can report back if they fit in a 23q presto.
-------------------- Signature text goes here. NTW's LAGM 2.023 P̫̻̳̮͎̥̬͋͊̂͐Ä̡͓̜̩̤̠̮́́̔̽̇Ṛ̹̱ͅA̠͉͒F̹̦̣͇̘̻̍̎ͬ͋͒̅͌͘I̖̻ͨͧ̇̋ͯ͡L̩̩͋́M͕̳͎̱̎ ̤̜͇̰̉̿ͫ̑͑͡ͅC̶̲̝̉̔U͚̙͕̬̘̗̐ͮ̀ͣ̎L̏́̂͋Ṯ̥̪̠ͩ͛̃͊
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tealseal
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Lmk if they fit and ill snag a few Thanks!
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NuclearTidalWave
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Re: i love glass petris & you can too!! [Re: tealseal]
#28110124 - 12/23/22 04:18 PM (1 year, 1 month ago) |
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This is with one row of mason jar lids below the trivet. Looks like I have about 10" of space. Seems like it would still work.
-------------------- Signature text goes here. NTW's LAGM 2.023 P̫̻̳̮͎̥̬͋͊̂͐Ä̡͓̜̩̤̠̮́́̔̽̇Ṛ̹̱ͅA̠͉͒F̹̦̣͇̘̻̍̎ͬ͋͒̅͌͘I̖̻ͨͧ̇̋ͯ͡L̩̩͋́M͕̳͎̱̎ ̤̜͇̰̉̿ͫ̑͑͡ͅC̶̲̝̉̔U͚̙͕̬̘̗̐ͮ̀ͣ̎L̏́̂͋Ṯ̥̪̠ͩ͛̃͊
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tealseal
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Omg im a dumbass.. this whole time i thought i had a 23q i deff do not as mine reads 9 inches inside... Its a 16 fuuuk lol
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NuclearTidalWave
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Re: i love glass petris & you can too!! [Re: tealseal]
#28110133 - 12/23/22 04:27 PM (1 year, 1 month ago) |
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As a fellow dumbass, I feel that. If you do figure out a good way to do no pours with glass petris I would be interested to hear about it though. I've been thinking about trying it out.
-------------------- Signature text goes here. NTW's LAGM 2.023 P̫̻̳̮͎̥̬͋͊̂͐Ä̡͓̜̩̤̠̮́́̔̽̇Ṛ̹̱ͅA̠͉͒F̹̦̣͇̘̻̍̎ͬ͋͒̅͌͘I̖̻ͨͧ̇̋ͯ͡L̩̩͋́M͕̳͎̱̎ ̤̜͇̰̉̿ͫ̑͑͡ͅC̶̲̝̉̔U͚̙͕̬̘̗̐ͮ̀ͣ̎L̏́̂͋Ṯ̥̪̠ͩ͛̃͊
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tealseal
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Quote:
NuclearTidalWave said: As a fellow dumbass, I feel that. If you do figure out a good way to do no pours with glass petris I would be interested to hear about it though. I've been thinking about trying it out.
Start by reading the beginning of this thread. Thats how hazy does it. I just dont like the tin foil bs. But with what u linked it can be avoided
Load the pc with the glass petris already poured and take it to the sab or ffu after n let the outside of the plates dry and wrap with cling.
The only thing i would add is those containers u linked may need a few pieces of MP tape on em to keep things sterile while removing them from the PC
Edited by tealseal (12/23/22 04:44 PM)
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Stipe-n Cap


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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28110153 - 12/23/22 04:38 PM (1 year, 1 month ago) |
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Nice work
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mixomatoz
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28159350 - 01/27/23 05:29 AM (1 year, 1 day ago) |
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Great infos thank you,
i just got mine, i was playing arround and i figure out something tell me if it will work.
i had used spawn bags, i clean them up,
then i put some glass petri in them, close them up with an impulse sealer, and i plan to PC them for 1h.
if it worked as intented it will got cleaned and bagged petri good for storage,
thoughts ?
edit: same idea but already with agar in it.?
Edited by mixomatoz (01/27/23 05:32 AM)
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Steevil
Registered: 01/21/03
Posts: 103
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Great write-up!
Quote:
NuclearTidalWave said: They make autoclaveable containers Like these
I was trying to find one of these for 60mm dishes and came across "stainless steel disinfection jars" (some places call them "cotton swab holders"). They're just stainless steel cans, they're much cheaper than the real deal.
They come in all different diameters and heights so they could fit in a regular PC. I'm guessing they won't seal tight. I've ordered a 65mm x 105mm can, I'll update with how it goes
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SirPsycho
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Re: i love glass petris & you can too!! [Re: Steevil] 1
#28162501 - 01/29/23 08:22 AM (11 months, 22 days ago) |
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Just before the new year I saw B Traven mention that they leave their no pour plates unwrapped after removing them from the pc and I decided to try it. Seems to be working pretty well. The first set I tried didn't have a chance to sit for very long so I couldn't really draw any conclusions but these two plates are from a batch that was removed from the pc on Jan 16th, so almost two weeks.
This is a picture of my culture drawer showing how I've been storing them. The red plates were done the day after the blue, Jan 17th. Haven't used any yet but they are still clean.

I still wrap with cling wrap or grafting tape after inoculation. Don't think B does though.
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28162951 - 01/29/23 02:03 PM (11 months, 22 days ago) |
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A look at a few more plates I transferred into today. Sitting unwrapped in my drawer since Jan 17
--------------------
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JHOVA
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28164429 - 01/30/23 01:52 PM (11 months, 21 days ago) |
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https://www.shroomery.org/forums/showflat.php/Number/27619322#27619322
Quote:
DERRAYLD said: I'm still on that no pour glass petri, I do them in large pp containers with massive polyfil stuffed lid, 10 petris at a time and leave them to cool inside the container. When I'm ready to work I open the large pp container in front of the flow.
I've been prepping dishes and leaving them for 3 weeks in the pp container and all traces of condensation dries off.
Just ordered some of these 75 mm plates to try out. Im gonna get a big pp5 container as the drying out box. Since these are so darn expensive they will be the final plates and holy grail for initial cleanup. Best of both worlds.
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LadysKnight
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Re: i love glass petris & you can too!! [Re: JHOVA]
#28164563 - 01/30/23 03:30 PM (11 months, 21 days ago) |
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The thin cheap soda/lime/Chinese glass plates work great and last forever as long as you treat them like glass. When I stocked up they were like $1.50ea, last I looked they were like $8ea. YMMV
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JHOVA
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Re: i love glass petris & you can too!! [Re: LadysKnight]
#28164636 - 01/30/23 04:18 PM (11 months, 21 days ago) |
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$2.20/each for 10.
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LadysKnight
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Re: i love glass petris & you can too!! [Re: JHOVA] 1
#28164994 - 01/30/23 08:23 PM (11 months, 21 days ago) |
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Not bad, but mine are 100s. I do have some 60s, but a procrastinator needs that extra time
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DERRAYLD
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Re: i love glass petris & you can too!! [Re: LadysKnight]
#28165328 - 01/31/23 02:36 AM (11 months, 21 days ago) |
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Glass plates have really gone up recently, boro has become cheaper than soda for some reason.
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Re: i love glass petris & you can too!! [Re: DERRAYLD] 1
#28168196 - 02/01/23 09:29 PM (11 months, 19 days ago) |
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First run. Tyvek + micropore tape in screw top pp5s.
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fiddle_head
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28168229 - 02/01/23 10:18 PM (11 months, 19 days ago) |
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Cool write-up Hazyhorse. I've been considering investin in some glass for some time now. Even if I don't use it all the time, I'd use it to showcase my more aesthetically pleasing/novel cultures, so I should at least have a few sitting around.
Quote:
JHOVA said:
 First run. Tyvek + micropore tape in screw top pp5s.
And that looks dope by the way. Let us know how that goes!
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SirPsycho
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Re: i love glass petris & you can too!! [Re: fiddle_head] 1
#28168594 - 02/02/23 06:25 AM (11 months, 18 days ago) |
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You guys are making this way too hard on yourself and I get it, I used to tape each individual plate with two layers of micropore before pressure cooking.
Just wrapping in foil then removing the foil after the pc and leaving them unwrapped has done great for me. Y'all should give it a shot.
How I wrap and the results after sitting in my drawer for two weeks are a few posts back in this thread.
--------------------
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johnukguy
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28168611 - 02/02/23 06:45 AM (11 months, 18 days ago) |
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Thank you for the excellent write up.

Thanks also to all of those contributing here - Sir Psycho, B.Traven, Kirkeng and others. I feel like this thread is just golden for some quality sharing.
-------------------- How to post pictures to shroomery TEK Shroomery Trusted Cultivator And Member YouTube Channels. “Evey Hammond: Who are you? V: Who? Who is but the form following the function of what and what I am is a man in a mask. Evey Hammond: Well I can see that. V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”
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KeyMaker
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Re: i love glass petris & you can too!! [Re: johnukguy]
#28168662 - 02/02/23 07:53 AM (11 months, 18 days ago) |
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I looked into this last year, never got around to it. I'm gonna do it now. I still have success with ketchup cups, although I did just order more plastic petris. I like the clarity.
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JHOVA
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28168667 - 02/02/23 07:57 AM (11 months, 18 days ago) |
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Quote:
SirPsycho said: You guys are making this way too hard on yourself and I get it, I used to tape each individual plate with two layers of micropore before pressure cooking.
Just wrapping in foil then removing the foil after the pc and leaving them unwrapped has done great for me. Y'all should give it a shot.
How I wrap and the results after sitting in my drawer for two weeks are a few posts back in this thread.
No thanks wrapping sucks.
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SirPsycho
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Re: i love glass petris & you can too!! [Re: JHOVA]
#28168672 - 02/02/23 08:02 AM (11 months, 18 days ago) |
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Quote:
JHOVA said:
Quote:
SirPsycho said: You guys are making this way too hard on yourself and I get it, I used to tape each individual plate with two layers of micropore before pressure cooking.
Just wrapping in foil then removing the foil after the pc and leaving them unwrapped has done great for me. Y'all should give it a shot.
How I wrap and the results after sitting in my drawer for two weeks are a few posts back in this thread.
No thanks wrapping sucks.
In what sense?
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28168674 - 02/02/23 08:05 AM (11 months, 18 days ago) |
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Contams. unfiltered air. Are you using large holy grails or glass petris? Totally different uses.
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SirPsycho
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Re: i love glass petris & you can too!! [Re: JHOVA]
#28168676 - 02/02/23 08:08 AM (11 months, 18 days ago) |
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Quote:
JHOVA said: Contams. unfiltered air. Are you using large holy grails or glass petris? Totally different uses.
Did you check the link, it shows exactly which plates I use; or my posts showing the unwrapped plates after two weeks, also shows the plates and complete absence of contam.
--------------------
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San Pedro Girl
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Re: i love glass petris & you can too!! [Re: SirPsycho] 2
#28168683 - 02/02/23 08:20 AM (11 months, 18 days ago) |
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Quote:
SirPsycho said: You guys are making this way too hard on yourself and I get it, I used to tape each individual plate with two layers of micropore before pressure cooking.
Just wrapping in foil then removing the foil after the pc and leaving them unwrapped has done great for me. Y'all should give it a shot.
How I wrap and the results after sitting in my drawer for two weeks are a few posts back in this thread.
Or ditch the foil and make it even easier!
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SirPsycho
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Re: i love glass petris & you can too!! [Re: San Pedro Girl]
#28168694 - 02/02/23 08:29 AM (11 months, 18 days ago) |
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Quote:
San Pedro Girl said:
Quote:
SirPsycho said: You guys are making this way too hard on yourself and I get it, I used to tape each individual plate with two layers of micropore before pressure cooking.
Just wrapping in foil then removing the foil after the pc and leaving them unwrapped has done great for me. Y'all should give it a shot.
How I wrap and the results after sitting in my drawer for two weeks are a few posts back in this thread.
Or ditch the foil and make it even easier!

That was gonna be my next purchase if the method I described before failed. I imagine they work great and do make it easier. You do have to be careful unwrapping the foil and putting them aside so you don't open them. I figure with these holders it's as easy as flipping upside down and sliding it off the stack.
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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Steevil
Registered: 01/21/03
Posts: 103
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28168701 - 02/02/23 08:36 AM (11 months, 18 days ago) |
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They are expensive, so I wonder how a $10 spaghetti tin would compare
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JHOVA
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28168702 - 02/02/23 08:38 AM (11 months, 18 days ago) |
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You keep posting holy grail pp5 screw tops in a glass petri thread. Why? I dont foil pp5s. I stack them up and pc them then screw em on tight post pc. No contams.
If you ever buy some glass petris you can see a huge gap between the ID and OD. its a big gap. SPG how much do those racks go for? Got a link?
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SirPsycho
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Re: i love glass petris & you can too!! [Re: JHOVA]
#28168703 - 02/02/23 08:39 AM (11 months, 18 days ago) |
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Quote:
JHOVA said: You keep posting holy grail pp5 screw tops in a glass petri thread. Why? I dont foil pp5s. I stack them up and pc them then screw em on tight post pc. No contams.
If you ever buy some glass petris you can see a huge gap between the ID and OD. its a big gap. SPG how much do those racks go for? Got a link?
They aren't screw top. They are petri dishes, 100mmx15mm
--------------------
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San Pedro Girl
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Re: i love glass petris & you can too!! [Re: SirPsycho] 1
#28168722 - 02/02/23 08:56 AM (11 months, 18 days ago) |
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Quote:
SirPsycho said:
Quote:
San Pedro Girl said:
Quote:
SirPsycho said: You guys are making this way too hard on yourself and I get it, I used to tape each individual plate with two layers of micropore before pressure cooking.
Just wrapping in foil then removing the foil after the pc and leaving them unwrapped has done great for me. Y'all should give it a shot.
How I wrap and the results after sitting in my drawer for two weeks are a few posts back in this thread.
Or ditch the foil and make it even easier!

That was gonna be my next purchase if the method I described before failed. I imagine they work great and do make it easier. You do have to be careful unwrapping the foil and putting them aside so you don't open them. I figure with these holders it's as easy as flipping upside down and sliding it off the stack.
I remove mine by turning sideways and pulling out with the cage since I have limited space in my SAB. It would be nice to have a flow hood so I could just lift them straight out without turning sideways or upside down.
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SirPsycho
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Re: i love glass petris & you can too!! [Re: San Pedro Girl]
#28168726 - 02/02/23 09:01 AM (11 months, 18 days ago) |
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I know you've answered this before but I forget, you're using 100mm plates right?
--------------------
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San Pedro Girl
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Re: i love glass petris & you can too!! [Re: Steevil] 1
#28168730 - 02/02/23 09:07 AM (11 months, 18 days ago) |
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Quote:
Steevil said: They are expensive, so I wonder how a $10 spaghetti tin would compare

Mine was only $24 total to my front door. You’ll need to drill a hole through the top/lid and you won’t have the cage to conveniently pull them out, but as long as the spaghetti barrel is stainless steel, it’ll probably work just fine.
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San Pedro Girl
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Re: i love glass petris & you can too!! [Re: SirPsycho] 1
#28168732 - 02/02/23 09:08 AM (11 months, 18 days ago) |
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Quote:
SirPsycho said: I know you've answered this before but I forget, you're using 100mm plates right?
Yes, 100mm x 15mm Pyrex brand.
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SirPsycho
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Re: i love glass petris & you can too!! [Re: San Pedro Girl]
#28168735 - 02/02/23 09:11 AM (11 months, 18 days ago) |
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Quote:
San Pedro Girl said:
Quote:
SirPsycho said: I know you've answered this before but I forget, you're using 100mm plates right?
Yes, 100mm x 15mm Pyrex brand.
Awesome, I may still get some just to eliminate all the foil I throw away after unwrapping.
--------------------
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28168753 - 02/02/23 09:21 AM (11 months, 18 days ago) |
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Quote:
SirPsycho said:
Quote:
San Pedro Girl said:
Quote:
SirPsycho said: I know you've answered this before but I forget, you're using 100mm plates right?
Yes, 100mm x 15mm Pyrex brand.
Awesome, I may still get some just to eliminate all the foil I throw away after unwrapping.
It’ll take quite a bit of foil to break even on the barrel, but for me it was just a quality of life upgrade.
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JHOVA
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Re: i love glass petris & you can too!! [Re: San Pedro Girl]
#28168794 - 02/02/23 09:45 AM (11 months, 18 days ago) |
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The pp5 bottles are $2.50/bottle, mp and tyvek from the post office.
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Re: i love glass petris & you can too!! [Re: JHOVA]
#28168845 - 02/02/23 10:16 AM (11 months, 18 days ago) |
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Quote:
JHOVA said: The pp5 bottles are $2.50/bottle, mp and tyvek from the post office.
Definitely a great option for anyone trying to save money.
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SirPsycho
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Re: i love glass petris & you can too!! [Re: San Pedro Girl] 1
#28168853 - 02/02/23 10:18 AM (11 months, 18 days ago) |
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They won't fit 100mm plates though, I've tried
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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KeyMaker
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28168867 - 02/02/23 10:23 AM (11 months, 18 days ago) |
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JHOVA
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28168881 - 02/02/23 10:34 AM (11 months, 18 days ago) |
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Quote:
SirPsycho said: They won't fit 100mm plates though, I've tried
They fit the 75mm plates ok. I did 5 each. I think it could fit 6.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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LadysKnight
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Re: i love glass petris & you can too!! [Re: Steevil]
#28168922 - 02/02/23 11:02 AM (11 months, 18 days ago) |
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Quote:
Steevil said: They are expensive, so I wonder how a $10 spaghetti tin would compare

I was gonna start looking for these, glad you beat me to it.
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Philbilly
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Re: i love glass petris & you can too!! [Re: LadysKnight]
#28169059 - 02/02/23 12:30 PM (11 months, 18 days ago) |
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Idk how many plates you PC at a time, but I just ordered 18 of those storage/ sterilization tubes off alibaba. They came to 15 a piece if I remember right. Comes with the rack and hole already in the lid. Added a case of their borosilicate glass plates for $120 so we will see how that goes.
Edited by Philbilly (02/02/23 12:32 PM)
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SirPsycho
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Re: i love glass petris & you can too!! [Re: JHOVA]
#28169449 - 02/02/23 04:30 PM (11 months, 18 days ago) |
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See homie, petri dishes. Just like you, except not glass cause that shit turns to 5 bucks a plate with shipping to America's Hat.

Gap and all. Actually "vented" too, if you zoom in at about 4-5 o'clock in the first pic you can see this little plastic line on the lid that keeps a slight gap between it and the bottom.
--------------------
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JHOVA
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Re: i love glass petris & you can too!! [Re: SirPsycho] 1
#28169463 - 02/02/23 04:41 PM (11 months, 18 days ago) |
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Thanks. It was confusing me to hell.
The price on alibaba per plate  The total cost with shipping
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Philbilly
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Re: i love glass petris & you can too!! [Re: JHOVA]
#28169623 - 02/02/23 06:35 PM (11 months, 18 days ago) |
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The shipping cost is 200 for 18 sterilization tubes, 144 glass Petri dishes, and some assorted other lab equipment. I’m almost convinced $200 shipping is a base price thing.
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Philbilly
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Re: i love glass petris & you can too!! [Re: San Pedro Girl]
#28169628 - 02/02/23 06:38 PM (11 months, 18 days ago) |
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My biggest selling point on the tubes, is that after you sterilize, you can just tape over the holes and around the lid to keep it sealed til you need it. Plus it should fit beside quart jars in the AA for a pc run
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San Pedro Girl
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Re: i love glass petris & you can too!! [Re: Philbilly]
#28169632 - 02/02/23 06:41 PM (11 months, 18 days ago) |
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Quote:
Philbilly said: My biggest selling point on the tubes, is that after you sterilize, you can just tape over the holes and around the lid to keep it sealed til you need it. Plus it should fit beside quart jars in the AA for a pc run
You don’t have to tape the hole. When you open the PC, just rotate the cap 180° to seal the holes.
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Steevil
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28170224 - 02/03/23 02:49 AM (11 months, 18 days ago) |
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Quote:
SirPsycho said:
 Gap and all. Actually "vented" too, if you zoom in at about 4-5 o'clock in the first pic you can see this little plastic line on the lid that keeps a slight gap between it and the bottom.
Yeah the pp5's have a little <1mm gap, and the plastic grips so they don't move at all when you carry a stack.
The glass ones have a 3mm gap - when you first see them it looks like then sent the wrong lid. They rattle around when you move them, it feels like they're drawing in air.
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SirPsycho
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Re: i love glass petris & you can too!! [Re: Steevil] 1
#28170505 - 02/03/23 09:15 AM (11 months, 17 days ago) |
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Quote:
Steevil said:
Quote:
SirPsycho said:
 Gap and all. Actually "vented" too, if you zoom in at about 4-5 o'clock in the first pic you can see this little plastic line on the lid that keeps a slight gap between it and the bottom.
Yeah the pp5's have a little <1mm gap, and the plastic grips so they don't move at all when you carry a stack.
The glass ones have a 3mm gap - when you first see them it looks like then sent the wrong lid. They rattle around when you move them, it feels like they're drawing in air.
I mean, it's clearly more than 1mm as the spine of my Leatherman fits in there but yes, I do imagine the plastic has higher static friction than glass.
The other thing to consider though is glass plates are generally deeper, 20mm was the most common I found.
Not really sure how to respond to "it feels" like it's drawing in air. Why would it? Is there a pressure difference?
Also, if you read my first post about leaving the dishes unwrapped you'll notice I mentioned I'm doing this at the suggestion of another user, B Traven. I'm pretty sure he's using glass plates.
We can discuss what we think will happen all day and not reach any conclusions. Just try it and see how it goes.
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Re: i love glass petris & you can too!! [Re: SirPsycho] 1
#28171866 - 02/04/23 10:14 AM (11 months, 16 days ago) |
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SirPsycho
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Re: i love glass petris & you can too!! [Re: JHOVA]
#28172032 - 02/04/23 12:48 PM (11 months, 16 days ago) |
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Quote:
JHOVA said:


Damn condensation
Have you tried leaving them unwrapped until use... It'll get rid of the condensation
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28172075 - 02/04/23 01:21 PM (11 months, 16 days ago) |
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Hindsight has a method that I haven't tried, but I believe it boils down to insulating them while hot so the agar can absorb the ambient moisture in the plate.
If that works, I wonder if you could just insulate the PC after it's no longer dangerous, for no pour.
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28172097 - 02/04/23 01:39 PM (11 months, 16 days ago) |
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Quote:
SirPsycho said:
Quote:
JHOVA said:


Damn condensation
Have you tried leaving them unwrapped until use... It'll get rid of the condensation 
next time. i can't spare the time to set them out to dry this round. i have the other 5 in the pp5 container i posted earlier so i'll see if that's enough to dry em out over the next couple days.
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Re: i love glass petris & you can too!! [Re: JHOVA]
#28173072 - 02/05/23 07:58 AM (11 months, 15 days ago) |
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My pyrex dish covers are heavy and the bases have a significant glass bead around the rim. Seems like a pretty solid seal to me. I imagine that a wider cover might actually help reduce turbulence and suction when removing it.
In addition to wrapping my no-pours in foil, I also engage in the antiquated practice of covering my jar lids with foil. Agar plate foil gets repurposed as jar lid foil. I end up with these multi-layer lid hats that eventually start to disintegrate.
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Re: i love glass petris & you can too!! [Re: B Traven]
#28173081 - 02/05/23 08:10 AM (11 months, 15 days ago) |
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Quote:
B Traven said: I imagine that a wider cover might actually help reduce turbulence and suction when removing it.
This. I have the cheap glass plates. They are not an exact, uniform product. When a plate has a snug fitting lid, it will not separate easily with one hand, especially when wet. This is why each plate has a dedicated, pre-selected lid with an acceptable gap.
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Re: i love glass petris & you can too!! [Re: San Pedro Girl] 1
#28175654 - 02/07/23 01:29 AM (11 months, 14 days ago) |
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took a bit of a break from posting & i'm glad to see this thread is still active!!
Quote:
Stipe-n Cap said: Nice work 
thanks dude! much appreciated.
Quote:
mixomatoz said: Great infos thank you, i had used spawn bags, i clean them up,
then i put some glass petri in them, close them up with an impulse sealer, and i plan to PC them for 1h.
if it worked as intented it will got cleaned and bagged petri good for storage,
thoughts ?
edit: same idea but already with agar in it.?
as long as the bag has a filter on it, i think this would work! so i actually PC'd in an oven bag (used foil to keep them sturdy) & left them on the shelf for about a month and a half. just opened the bag tonight & all the plates are clean! i just made sure to lightly knot the oven bag so that air could escape in the PC, then immediately tightened the knot when i pulled the bag out of the PC. the top agar plates were actually a bit dry, but nothing had contaminated. kinda figured it would be fine, but now i have confirmation. i don't think i'll be storing any plates like this tbh but i could see this being useful if you had an SAB but still wanted to do a bunch of plates all at once.
  
one of the bottom plates took in a bit of water at some point and boiled out into the bag, but other than staining the plastic nothing came of that. you can see that weird dry agar texture in that one plate, but there was no sign of bacteria at all.
Quote:
Steevil said: Great write-up!
Quote:
NuclearTidalWave said: They make autoclaveable containers Like these
I was trying to find one of these for 60mm dishes and came across "stainless steel disinfection jars" (some places call them "cotton swab holders"). They're just stainless steel cans, they're much cheaper than the real deal.
They come in all different diameters and heights so they could fit in a regular PC. I'm guessing they won't seal tight. I've ordered a 65mm x 105mm can, I'll update with how it goes 
thanks! how did these work out? i saw all the PP5 stuff & thought about it, i'll have to try once i have some empty bottles.
Quote:
SirPsycho said: You guys are making this way too hard on yourself and I get it, I used to tape each individual plate with two layers of micropore before pressure cooking.
Just wrapping in foil then removing the foil after the pc and leaving them unwrapped has done great for me. Y'all should give it a shot.
How I wrap and the results after sitting in my drawer for two weeks are a few posts back in this thread.
i'm really shocked they didn't contam, that's some wild news!! i just unwrapped a plate & am gonna see how my glass plates do. mine are the really loose fitting kind from amazon. i'll update if anything happens in the next few weeks.
Quote:
San Pedro Girl said: Or ditch the foil and make it even easier!

i'd love one of these, but i couldn't find any that were short enough to work in my 16qt cooker /:
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28175782 - 02/07/23 06:23 AM (11 months, 13 days ago) |
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Quote:
hazyhorse said:
Quote:
Steevil said: Great write-up!
Quote:
NuclearTidalWave said: They make autoclaveable containers Like these
I was trying to find one of these for 60mm dishes and came across "stainless steel disinfection jars" (some places call them "cotton swab holders"). They're just stainless steel cans, they're much cheaper than the real deal.
They come in all different diameters and heights so they could fit in a regular PC. I'm guessing they won't seal tight. I've ordered a 65mm x 105mm can, I'll update with how it goes 
thanks! how did these work out? i saw all the PP5 stuff & thought about it, i'll have to try once i have some empty bottles.
I'm not sure yet. I got tired of waiting for delivery and bought this tin from a local coffee shop:

After some encouragement with a hammer it snugly fits 5x 60x18mm dishes. I did a dry sterilization test run for 30mins at 180°C with no problem, but I'll have to wait and see if dishes contaminate more than with foil. Next time I have empty dishes I'll try a no-pour PC run. I see the proper ones have holes that can align to allow steam in, so I'll drill some of those.
Basically I think I'm going to like using it, but I wouldn't recommend it to anyone else, if you know what I mean. Like if you really can't get a real one or you really want to put it in a PC then get one, but in the end you might still prefer foil.
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Re: i love glass petris & you can too!! [Re: Steevil]
#28175799 - 02/07/23 06:50 AM (11 months, 13 days ago) |
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Quote:
Steevil said:
Quote:
hazyhorse said:
Quote:
Steevil said: Great write-up!
Quote:
NuclearTidalWave said: They make autoclaveable containers Like these
I was trying to find one of these for 60mm dishes and came across "stainless steel disinfection jars" (some places call them "cotton swab holders"). They're just stainless steel cans, they're much cheaper than the real deal.
They come in all different diameters and heights so they could fit in a regular PC. I'm guessing they won't seal tight. I've ordered a 65mm x 105mm can, I'll update with how it goes 
thanks! how did these work out? i saw all the PP5 stuff & thought about it, i'll have to try once i have some empty bottles.
I'm not sure yet. I got tired of waiting for delivery and bought this tin from a local coffee shop:

After some encouragement with a hammer it snugly fits 5x 60x18mm dishes. I did a dry sterilization test run for 30mins at 180°C with no problem, but I'll have to wait and see if dishes contaminate more than with foil. Next time I have empty dishes I'll try a no-pour PC run. I see the proper ones have holes that can align to allow steam in, so I'll drill some of those.
Basically I think I'm going to like using it, but I wouldn't recommend it to anyone else, if you know what I mean. Like if you really can't get a real one or you really want to put it in a PC then get one, but in the end you might still prefer foil.
Drill a small hole straight through the lid and top of the container. When you use it in the PC, it will allow steam to penetrate well. That’s one of its main advantages over foil. When you remove it from the PC, just twist the cap 180° to offset the holes and seal it.
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Re: i love glass petris & you can too!! [Re: San Pedro Girl]
#28175823 - 02/07/23 07:14 AM (11 months, 13 days ago) |
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Im still testing with the pp5s. I used a flathead screwdriver which made a tiny hole with tyvek and mp on both the inside and outside. The air flow is far too restricted. For now i removed the outside tape combo.
Next time ill make at least a quarter inch hole for better drying.
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28175894 - 02/07/23 08:04 AM (11 months, 13 days ago) |
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I was surprised as well but it seems to be consistent so far. Got another set of 24 I did on the 26th and just started using, all look clean. A set I did last week and making more tonight
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Re: i love glass petris & you can too!! [Re: SirPsycho] 1
#28176471 - 02/07/23 04:20 PM (11 months, 13 days ago) |
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I bought a set of cheap glass petri and a set of the pp5 sirpsycho uses.
The cheap glass petris were really hard for me to wrap with parafilm because of the big lid/base gap. On three plates, the parafilm just slid off one side exposing the gap; one now has a little spot of penicillium contam.
They're not smooth/uniform thickness, so the visibility isn't great, kind of like a fun house mirror.
And, the condensation is crazy. I left them 60 hours in the SAB and still had bad pooling. I needed the SAB, so I had to take them out. I inoculated them anyway, which was probably a mistake.
The pp5 seemed to fare better with condensation, not sure why. I used charcoal in that batch. They're also easier to wrap with parafilm.
I'm picking up a set of pyrex 100x15mm and will see if the results are better.
In the meantime, any tips on dealing with the condensation? I used D3 recipe linked in the OP, same brand of agar, with a stick blender. I dried in stacks of 5 in the SAB, but it was a bit cold, like 16-17C.
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Re: i love glass petris & you can too!! [Re: Moopers]
#28176485 - 02/07/23 04:34 PM (11 months, 13 days ago) |
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Quote:
Moopers said: I bought a set of cheap glass petri and a set of the pp5 sirpsycho uses.
The cheap glass petris were really hard for me to wrap with parafilm because of the big lid/base gap. On three plates, the parafilm just slid off one side exposing the gap; one now has a little spot of penicillium contam.
They're not smooth/uniform thickness, so the visibility isn't great, kind of like a fun house mirror.
And, the condensation is crazy. I left them 60 hours in the SAB and still had bad pooling. I needed the SAB, so I had to take them out. I inoculated them anyway, which was probably a mistake.
The pp5 seemed to fare better with condensation, not sure why. I used charcoal in that batch. They're also easier to wrap with parafilm.
I'm picking up a set of pyrex 100x15mm and will see if the results are better.
In the meantime, any tips on dealing with the condensation? I used D3 recipe linked in the OP, same brand of agar, with a stick blender. I dried in stacks of 5 in the SAB, but it was a bit cold, like 16-17C.
I have Pyrex petris, 100mm x 15mm, and use parafilm M. I pour at 60°C and only have condensation issues on the top plate. However, that’s fixed within a couple days. If you have condensate issues, flip the plate over. If the parafilm slips off the plate, you’re either not stretching it enough or the plate is wet.
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Re: i love glass petris & you can too!! [Re: San Pedro Girl]
#28176505 - 02/07/23 04:52 PM (11 months, 13 days ago) |
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Stretching it over the 3mm gap is the issue, I think. I either pull too hard (it breaks, gets too narrow to cover the gap or just barely covers it) or not hard enough (wide enough to cover gap, not stretched enough to be sticky). Maybe the outsides were still a little wet, too. I'll look out for that next time. Thanks for the tips.
Never had these issues with PS petris, and the pp5 were much easier.
I'm hoping things go better with the Pyrex.
Edited by Moopers (02/07/23 04:55 PM)
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Re: i love glass petris & you can too!! [Re: Moopers] 2
#28176576 - 02/07/23 06:05 PM (11 months, 13 days ago) |
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Yes, yes. Embrace the pp, love the pp
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Re: i love glass petris & you can too!! [Re: Moopers]
#28177931 - 02/08/23 04:12 PM (11 months, 12 days ago) |
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yo what up moopers!!
for wrapping my petris individually, i use an extra wide cut off a roll of cling wrap. i had considered trying out parafilm but kind of assumed it wouldn’t be wide enough for the gap on the cheap plates because it all looks a lot more narrow than what i use.
i’ve gotten lucky with my plates in general, but yeah quality varies really wildly with them. i have one that doesn’t sit flat because the bottom part of the plate has a huge bubble of glass lol. almost all mine are really clear to look through though, so maybe i got lucky there.
very weird about the condensation. if they are wet at all on they outside they are almost impossible to wrap ime. i take it you took them out of the foil to dry? was your SAB totally sealed while they were drying? unless you live in a super humid area i can’t see why it would take so long. maybe cold temps affected that but idk, 60 hours should be plenty of time… mine were always good after. a little condensation is pretty hard to avoid but i don’t really care about it as usually they clear up when in storage. only other thing i can think of is if you put the lids on them while the agar was still hot? i let my plates completely cool before adding the lids & stacking for the PC
if i find myself in need of more petris i’ll have to try out the pp5s, they sound sick. but i’ve had good luck with my glsss so far
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28180160 - 02/09/23 08:45 PM (11 months, 11 days ago) |
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I would just like to add that the name brands are the best for handling. The Pyrex and Klimax both have beaded rims, which make them stronger and more break resistant. Secondly the name brands have a cover that its shorter than the dish which makes handling, opening, and wrapping much easier.
I'll give this no pour another try. I have stainless sleeves that I can PC which has a rack inside to hold them. I did it with the two holes open, so next time, I'll keep those closed so the plates are not so wet. Also, had to modify the base so that my agar was not slanted.
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Re: i love glass petris & you can too!! [Re: MRB3] 1
#28180216 - 02/09/23 09:39 PM (11 months, 11 days ago) |
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having a level surface/PC to cool the cooker on is so important with the no pours. i’ve had some funky ass slants before i flattened out my PC bottom (im dumb & warped it very early on in my cultivation journey lol)
i would absolutely love some pyrex dishes but there’s no chance i have the money anytime soon. they look so beautiful though
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28180442 - 02/10/23 06:09 AM (11 months, 10 days ago) |
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Hazy, I did the same thing with my PCer. What did you use to flatten the bottom back out? I've been thinking about doing the same, but I don't want to damage it.
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Re: i love glass petris & you can too!! [Re: ruawakeyet] 1
#28180454 - 02/10/23 06:20 AM (11 months, 10 days ago) |
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i’d venture to guess a lot of people here have at least had a close call or two lol. i used an old ski deck, laid it across the PC bottom & hit it with a rubber mallet, but if you have a flat piece of wood that would probably be better. i can’t really advocate for the safety of doing this obviously but my PC has been doing fine for awhile now
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28180950 - 02/10/23 01:33 PM (11 months, 10 days ago) |
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Quote:
hazyhorse said:very weird about the condensation. if they are wet at all on they outside they are almost impossible to wrap ime. i take it you took them out of the foil to dry? was your SAB totally sealed while they were drying? unless you live in a super humid area i can’t see why it would take so long. maybe cold temps affected that but idk, 60 hours should be plenty of time… mine were always good after. a little condensation is pretty hard to avoid but i don’t really care about it as usually they clear up when in storage. only other thing i can think of is if you put the lids on them while the agar was still hot? i let my plates completely cool before adding the lids & stacking for the PC
Foil was off the plates, and they were in stacks of 5 (glass) or 6 (PP5).
I waited for the agar to cool before putting the lids on to PC.
SAB was totally sealed. My SAB is a tote placed upside-down on its lid, with a towel on the lid so the air gap is sealed. I covered the atm holes with foil, taped on.
Maybe I should skip the towel next time, so there's a little air gap? Do you spray your SAB down and spray inside to settle the air before using it to dry the plates? I did this as a matter of course, but maybe it made it too humid inside.
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Re: i love glass petris & you can too!! [Re: Moopers] 2
#28180995 - 02/10/23 02:02 PM (11 months, 10 days ago) |
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i think the problem was that it was totally sealed. if there wasn’t any air exchange i could see it staying way too moist in there. i use a flowhood now, but when i used my SAB i would generally just do a quick iso wipe or leave it dry, leave the foil wrapped plates in there for 20minutes or so to let the air settle & then would carefully unwrap. i would mist a little sometimes but i made sure to not spray the walls to heavily (i also have a very fine hair dresser mister so i wasn’t blasting huge drops all over it). i think the foil over the armholes was probably the worst offender of keeping moisture in. i usually just draped a clean tshirt or two over the arm holes & pushed it in to the holes slightly so that there wasn’t any free flowing air going in. i think that helped facilitate a bit more evaporation
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Re: i love glass petris & you can too!! [Re: MRB3]
#28181011 - 02/10/23 02:14 PM (11 months, 10 days ago) |
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Quote:
MRB3 said: I would just like to add that the name brands are the best for handling. The Pyrex and Klimax both have beaded rims, which make them stronger and more break resistant. Secondly the name brands have a cover that its shorter than the dish which makes handling, opening, and wrapping much easier.
I'll give this no pour another try. I have stainless sleeves that I can PC which has a rack inside to hold them. I did it with the two holes open, so next time, I'll keep those closed so the plates are not so wet. Also, had to modify the base so that my agar was not slanted.
Where did you get the stainless sleeves with a rack? Is it a kitchen tool?
-------------------- #1 Tek With utmost care and precision,I work to achieve my fungal mission. Sterilizing all my tools and space,to ensure not a single spore is out of place. I carefully select the fungal strain and onto the agar, I make it rain. With a steady hand and gentle touch, I inoculate the plate without a hitch. Success is sweet when it's pure and true, and my agar plates are a testament too. A job well done, with no contamination, my fungi inoculation, a thing of admiration. JK NO --Bacteriological Virtuoso aka Master of Mold
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Moopers
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28181083 - 02/10/23 03:06 PM (11 months, 10 days ago) |
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Quote:
hazyhorse said: i think the problem was that it was totally sealed. if there wasn’t any air exchange i could see it staying way too moist in there. i use a flowhood now, but when i used my SAB i would generally just do a quick iso wipe or leave it dry, leave the foil wrapped plates in there for 20minutes or so to let the air settle & then would carefully unwrap. i would mist a little sometimes but i made sure to not spray the walls to heavily (i also have a very fine hair dresser mister so i wasn’t blasting huge drops all over it). i think the foil over the armholes was probably the worst offender of keeping moisture in. i usually just draped a clean tshirt or two over the arm holes & pushed it in to the holes slightly so that there wasn’t any free flowing air going in. i think that helped facilitate a bit more evaporation
Thanks, these are great tips for a noob to homemade agar, like me. You should consider adding them to your write-up!
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hazyhorse
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Re: i love glass petris & you can too!! [Re: Moopers] 1
#28181107 - 02/10/23 03:27 PM (11 months, 10 days ago) |
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i’m always happy to help!! it’s definitely some stuff that may not be super obvious to people new to it but is easy to gloss over when you’ve already got an understanding. probably wouldn’t hurt to add the info into the main post, thanks for the idea (:
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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SirPsycho
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28181279 - 02/10/23 05:57 PM (11 months, 10 days ago) |
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Unwrapped plates still holding up, third set to reach two weeks sitting in my drawer.
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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hazyhorse
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Re: i love glass petris & you can too!! [Re: SirPsycho] 1
#28181286 - 02/10/23 06:05 PM (11 months, 10 days ago) |
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hell yeah dude that’s bad ass. my test plate is looking clean so far but it’s only been a few days. i’ll update accordingly
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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JHOVA
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Re: i love glass petris & you can too!! [Re: JHOVA] 1
#28182345 - 02/11/23 01:12 PM (11 months, 9 days ago) |
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Quote:
JHOVA said:


Damn condensation
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hazyhorse
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Re: i love glass petris & you can too!! [Re: JHOVA]
#28182375 - 02/11/23 01:24 PM (11 months, 9 days ago) |
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looking a bit better with the condensation!! i’ve found ambient temps really affect the amount of condensation on my glass plates too. some days they are crystal clear & others they are obscured by condensation if i don’t move them to a warmer spot
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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LotKid
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Re: i love glass petris & you can too!! [Re: hazyhorse] 7
#28182897 - 02/11/23 07:08 PM (11 months, 9 days ago) |
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nice to see ppl reducing their single use plastic waste.
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JHOVA
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Re: i love glass petris & you can too!! [Re: LotKid] 1
#28183304 - 02/12/23 12:05 AM (11 months, 9 days ago) |
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Thanks man. The ones in a pp5 are clearing up. Im gonna test leaving them in a shoe box in my closet next; Sirpyscho style.
Still trying to figure out how to get good photos with optimal lighting.
The clarity even with some condensation is amazing!
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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hazyhorse
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Re: i love glass petris & you can too!! [Re: LotKid] 3
#28184315 - 02/12/23 04:15 PM (11 months, 8 days ago) |
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amen to that. feels good to keep plastic out of landfills. idk if i’ll ever feel comfortable enough to not wrap my dishes, but sir p’s results are looking super promising. my plate has remained clean so far, but a few weeks is gonna be the real test. would be great to not have to toss away wrap if i don’t have to.
@JHOVA: dude the visibility in the glass petris is top notch. like you said, even with a bit of condensation it kicks the shit out of a traditional no-pour
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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Leftfield420
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Re: i love glass petris & you can too!! [Re: LotKid] 1
#28184471 - 02/12/23 06:31 PM (11 months, 8 days ago) |
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I've tried glass petris and I broke almost all of them, and sent the remaining ones to a member here from canada... I'm rockin the celltreat petri dishes w/grip ring, anything else pisses me off to mess with🤣🤣.... started pouring plates with these and probably won't quit using them
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SirPsycho
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Re: i love glass petris & you can too!! [Re: Leftfield420] 2
#28184474 - 02/12/23 06:34 PM (11 months, 8 days ago) |
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--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Leftfield420
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28184485 - 02/12/23 06:41 PM (11 months, 8 days ago) |
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🤣🤣... I was using glass back in 2016, I'm too clumbsy for glass
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dowodenum
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Re: i love glass petris & you can too!! [Re: Leftfield420]
#28184628 - 02/12/23 08:38 PM (11 months, 8 days ago) |
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Were they flint glass? Borosilicate is a lot stronger... I've dropped one in the sink and it survived. Price ain't right though, so whatever works!
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 🅃 🄴 🄰 🄼 🄲 🅄 🅁 🄸 🄾 🅄 🅂
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SirPsycho
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Re: i love glass petris & you can too!! [Re: dowodenum] 2
#28184687 - 02/12/23 09:44 PM (11 months, 8 days ago) |
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Y'all know what don't break when you drop it? PP5
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Shrimps
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Re: i love glass petris & you can too!! [Re: SirPsycho] 3
#28184926 - 02/13/23 02:23 AM (11 months, 8 days ago) |
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Can you guys please not ruin Hazy's thread? It's about loving glass petris and not about other options. We all now that pp5 works, but that's not the topic of this thread. So please keep it together guys
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fiddle_head
I'm not the dude, guy



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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28184929 - 02/13/23 02:27 AM (11 months, 8 days ago) |
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Quote:
SirPsycho said: Y'all know what don't break when you drop it? PP5

I see you slur
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LotKid
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Re: i love glass petris & you can too!! [Re: Leftfield420] 1
#28185293 - 02/13/23 10:45 AM (11 months, 7 days ago) |
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Quote:
Leftfield420 said: I've tried glass petris and I broke almost all of them, and sent the remaining ones to a member here from canada... I'm rockin the celltreat petri dishes w/grip ring, anything else pisses me off to mess with🤣🤣.... started pouring plates with these and probably won't quit using them
did you get real dishes or the cheap amazon bullshit ones?
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Leftfield420
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Re: i love glass petris & you can too!! [Re: LotKid]
#28185434 - 02/13/23 12:38 PM (11 months, 7 days ago) |
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kimex petris... pretty sure I spent a couple hundred dollars on a case of like 50
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LotKid
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Re: i love glass petris & you can too!! [Re: Leftfield420]
#28185541 - 02/13/23 01:36 PM (11 months, 7 days ago) |
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KeyMaker
Urban Wizard

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Re: i love glass petris & you can too!! [Re: LotKid]
#28188096 - 02/15/23 05:04 AM (11 months, 6 days ago) |
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Wait is that good or bad lol
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Leftfield420
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Re: i love glass petris & you can too!! [Re: KeyMaker] 1
#28188106 - 02/15/23 05:30 AM (11 months, 6 days ago) |
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pretty sure that is the "it's a damn shame" look 🤣🤣
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KeyMaker
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Re: i love glass petris & you can too!! [Re: Leftfield420]
#28188114 - 02/15/23 05:45 AM (11 months, 6 days ago) |
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So what are the real ones, Pyrex?
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Leftfield420
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Re: i love glass petris & you can too!! [Re: KeyMaker]
#28188130 - 02/15/23 05:59 AM (11 months, 6 days ago) |
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kimex and pyrex are good dishes... you can get cheap ones from amazon that are shit and break if you look at them wrong
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Shrimps
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Re: i love glass petris & you can too!! [Re: Leftfield420]
#28188133 - 02/15/23 06:06 AM (11 months, 5 days ago) |
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Boro is great
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hazyhorse
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Re: i love glass petris & you can too!! [Re: Shrimps]
#28188720 - 02/15/23 01:31 PM (11 months, 5 days ago) |
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the cheap amazon ones are fine if you just get good (; surprisingly have only broken like two out of the 50 i have
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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hazyhorse
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Re: i love glass petris & you can too!! [Re: hazyhorse] 3
#28200853 - 02/23/23 03:16 PM (10 months, 28 days ago) |
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just did a run using the ziplock pp5s & i don’t think i’m going back to foil. this made the “unwrapping” process so much easier & it’s nice to have the reusability of plastic. they fit my 75mm dishes perfectly too & with stacks of 6 i can do a fair bit at a time. i think i’ll add some polyfil filters on the top (i guess the bottom technically?) & have some dedicated bottles for plates. i might update the OP & recommend this over foil; i think this would be a really great way to do these plates in the SAB. way less air disturbance & you can let them dry on the shelf if you really want
also, my unwrapped petri showed a contam a day or two ago. i do have to mention that it did get bumped pretty aggressively & the lid didn’t fully come off but moved enough to cause some air to get in. before that tho i was picking it up every day & moving it around & it looked clean. im gonna do another test next time i make agar bc i think it’s viable, but, at least with the glass plates i have, they require a bit more careful handling. i’ll probably keep wrapping my plates just because i don’t want some random contam to fuck me over but i think it’s cool that there’s the option of storing without wrapping. thank you for doing some experimenting with that sir p! it’s excellent info to have on the thread & i really appreciate how much you’ve added to it
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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B Traven
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28200861 - 02/23/23 03:34 PM (10 months, 28 days ago) |
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Quote:
hazyhorse said:
 
just did a run using the ziplock pp5s & i don’t think i’m going back to foil. this made the “unwrapping” process so much easier & it’s nice to have the reusability of plastic. they fit my 75mm dishes perfectly too & with stacks of 6 i can do a fair bit at a time. i think i’ll add some polyfil filters on the top (i guess the bottom technically?) & have some dedicated bottles for plates. i might update the OP & recommend this over foil; i think this would be a really great way to do these plates in the SAB. way less air disturbance & you can let them dry on the shelf if you really want
also, my unwrapped petri showed a contam a day or two ago. i do have to mention that it did get bumped pretty aggressively & the lid didn’t fully come off but moved enough to cause some air to get in. before that tho i was picking it up every day & moving it around & it looked clean. im gonna do another test next time i make agar bc i think it’s viable, but, at least with the glass plates i have, they require a bit more careful handling. i’ll probably keep wrapping my plates just because i don’t want some random contam to fuck me over but i think it’s cool that there’s the option of storing without wrapping. thank you for doing some experimenting with that sir p! it’s excellent info to have on the thread & i really appreciate how much you’ve added to it
That is rad.
It would be great to get to a point where I'm using zero consumable packaging (don't wrap plates after inoculating, but I do wrap them in foil). Definitely some food for thought there.
-------------------- Beware of advice- even this.
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hazyhorse
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Re: i love glass petris & you can too!! [Re: B Traven] 1
#28200904 - 02/23/23 04:08 PM (10 months, 28 days ago) |
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im fully with ya. it’s nice to be zero waste with shit if i can be & this method is one step closer (thx derrayld & others who mentioned this method!)
if you wanted to avoid wrapping but still wanted them to be protected after using them for transfers & stuff i could see using pp5s/metal containers like this to store dishes during colonization. like only open & close in SAB or in front of the flow hood. it would make it kind of annoying to check on cultures or just pick one out for transfers tho. maybe the smaller containers like this could be used just for storage of a few dishes or specific culture something
or pony up, raw dog & just leave em unwrapped in the open lol
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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SirPsycho
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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28200906 - 02/23/23 04:09 PM (10 months, 28 days ago) |
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--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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San Pedro Girl
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28200915 - 02/23/23 04:14 PM (10 months, 28 days ago) |
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Quote:
hazyhorse said: im fully with ya. it’s nice to be zero waste with shit if i can be & this method is one step closer (thx derrayld & others who mentioned this method!)
if you wanted to avoid wrapping but still wanted them to be protected after using them for transfers & stuff i could see using pp5s/metal containers like this to store dishes during colonization. like only open & close in SAB or in front of the flow hood. it would make it kind of annoying to check on cultures or just pick one out for transfers tho. maybe the smaller containers like this could be used just for storage of a few dishes or specific culture something
or pony up, raw dog & just leave em unwrapped in the open lol
Not criticizing, but just curious. What do you all have against throwing out parafilm or cling wrap after a single use?
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B Traven
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Re: i love glass petris & you can too!! [Re: San Pedro Girl] 5
#28200950 - 02/23/23 04:30 PM (10 months, 28 days ago) |
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I'd like to get to zero waste if possible. Both as a personal matter and as a way to develop more sustainable techniques for the future.
I compost all of my biological materials already (spent substrate, agar, etc.)
Personally, I don't have a background in microbiology and never got the memo about wrapping petri dishes. I started off with pp5 containers, which are still in the mix, and half-pint jars and whatnot. When I finally stepped up to use an old stash of pyrex petris a friend had given me, I didn't know about wrapping them and never started. I was several years into glass petri world when I realized that most people wrap their plates. I don't know if it's particular to the glass petris I have, or the way I store them, but keeping my plates unwrapped has never been an issue for me, so I never felt the need to start. Part of it is just not wanting to switch up my routines, didn't want to add another variable to the process.
To put things in historical perspective, I let those glass petris sit in a bucket in my back yard for years before cleaning them up and using them. Making pastyplates from pp5 containers all that time. Why? Because Roger Rabbit posted a bunch about how much glass sucks for mycology, and about how disposable plastic plates are the best option. No way in hell I was going that route, as it seemed over-the-top wasteful to me. So I found the pastyplate tek and went with that. Not realizing I was sitting on hundreds of dollars worth of glassware that a lot of people would do extreme things for. (Of course now, when I'm in peak agar mode, I need all my glass petris AND all my pp5's lol)
-------------------- Beware of advice- even this.
Edited by B Traven (02/23/23 05:45 PM)
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hazyhorse
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Re: i love glass petris & you can too!! [Re: San Pedro Girl] 1
#28201670 - 02/23/23 10:52 PM (10 months, 28 days ago) |
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Quote:
San Pedro Girl said: Not criticizing, but just curious. What do you all have against throwing out parafilm or cling wrap after a single use?
i have nothing against it at all! i think in my earlier post i said i was planning on wrapping my dishes still just to be safe. i don’t judge anyone for using disposable petris or bags or anything like that, individual action is unfortunately a drop in the bucket when it comes to lessening waste & all things have pros & cons that weight differently for different people. but if i can minimize how much plastic im burning through without making my workflow & results suffer i’d like to because why not? it’s one less thing to have to constantly buy & it’s one molecule of water less going back into the bucket. & if people do wanna go the zero waste method i think it’s good to let them know they have some options which is why i’m interested in seeing if wrapping is something that can be eliminated if someone would like to do so
@b traven: well written post as always. the pyrex dishes seem to have a tighter tolerance so they may work better unwrapped than the amazon plates, but even the one test plate i did only showed any contam after i fumbled it a bit. it’s a shame RR & some other members shit talked them so much in the earlier days of cultivation because i pretty much did the same thing (was not lucky enough to be sitting on a ton of pyrex plates, though ;p). once i decided to take the plunge & get some glass plates i realized they actually kinda kick ass, especially for no pour containers. i feel that about having all hands on deck with agar, i’ve def had to bust out the pasty plates every now & again lol. i finally have a big enough stockpile of glass that i don’t need to, but they were so nice to have on hand
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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JHOVA
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28202559 - 02/24/23 01:18 PM (10 months, 27 days ago) |
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Nice hazy. Mine dried up once i removed the underside mp/tyvek combo. I left the top mp/tyvek and they all look perfect.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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eu-da-mon
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28261818 - 04/04/23 10:36 AM (9 months, 19 days ago) |
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How are folks labeling their glass petris? China marker seems to be pulled off by the saran wrap on the plate stacked above, and sharpie becomes a vague smear after the first iso spritz.
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San Pedro Girl
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Re: i love glass petris & you can too!! [Re: eu-da-mon]
#28261823 - 04/04/23 10:42 AM (9 months, 19 days ago) |
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Quote:
eu-da-mon said: How are folks labeling their glass petris? China marker seems to be pulled off by the saran wrap on the plate stacked above, and sharpie becomes a vague smear after the first iso spritz.
Masking tape. Then you can just transfer the tape to the grain jar, then to the tub.
However, no need to spray your plates with iso. Also, I put my plates in bags now so I just sharpie on those. Whatever works for you.
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NFLProof
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Re: i love glass petris & you can too!! [Re: San Pedro Girl] 2
#28261950 - 04/04/23 12:15 PM (9 months, 19 days ago) |
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Quote:
San Pedro Girl said:
Quote:
eu-da-mon said: How are folks labeling their glass petris? China marker seems to be pulled off by the saran wrap on the plate stacked above, and sharpie becomes a vague smear after the first iso spritz.
Masking tape. Then you can just transfer the tape to the grain jar, then to the tub.
However, no need to spray your plates with iso. Also, I put my plates in bags now so I just sharpie on those. Whatever works for you.
Write it on the bag....so simple... Jesus.
-------------------- Yeah, yeah.
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SirPsycho
Purple Belt in Google-Fu



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Re: i love glass petris & you can too!! [Re: eu-da-mon] 3
#28262192 - 04/04/23 02:31 PM (9 months, 19 days ago) |
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Quote:
eu-da-mon said: How are folks labeling their glass petris? China marker seems to be pulled off by the saran wrap on the plate stacked above, and sharpie becomes a vague smear after the first iso spritz.
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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Yuggoth
Mi-Go Cultivator


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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28307862 - 05/05/23 01:57 PM (8 months, 19 days ago) |
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I posted some first impressions of the cheap Scientifics Direct glass petris in the "what new toys did you get" thread.
https://www.shroomery.org/forums/showflat.php/Number/28307857#28307857
tl;dr -- they seem OK but I do not have any of the slightly more expensive Karter petris for comparison.
-------------------- We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo
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hazyhorse
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Re: i love glass petris & you can too!! [Re: Yuggoth] 4
#28340945 - 05/30/23 06:17 PM (7 months, 25 days ago) |
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gonna update the OP a bit too, but i've been using these bad boys for a few agar runs now & have been happy with em. changed them up a little from my last post about them

there is unfortunately some headspace lost to a slight taper, which leaves me able to do 6 plates at a time when i probably could fit 8 in there otherwise, but it's not a big enough issue for me to work around it yet. i made about 6 holes, may bump it to 8 just to help with drying times, but they seem to dry out well over a few days on the shelf. i also added a thin strip of foil under the bottom petri so i could pick the whole stack up by just pinching the sides of the foil, just makes that bottom plate way easier to handle
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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Edited by hazyhorse (05/30/23 06:28 PM)
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MoJim
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28371147 - 06/23/23 09:34 AM (7 months, 1 day ago) |
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Ok, I'm convinced. Just ordered "vintage excellent condition" Pyrex USA dishes. 10 for $50 Ebay. Can't wait to try them out.
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Arctic_Fox
Mr. Furious


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Re: i love glass petris & you can too!! [Re: hazyhorse] 2
#28427476 - 08/09/23 06:30 PM (5 months, 16 days ago) |
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I'd like to share a tip for spotless glass petri dishes.
I eliminated water spots on my petri dishes by keeping a container of distilled water like this next to the sink, and dropping them in after washing. Then I take them out, and they are spotless after air drying.

I also use nitrile gloves for washing them to avoid fingerprints.
I found no issues with leaving the water in the container for a week and reusing it for a few rounds of washing 10 or so dishes. If it starts leaving residues or getting soapy, change it.
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jay.ach
Traveler



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Re: i love glass petris & you can too!! [Re: Arctic_Fox]
#28427684 - 08/09/23 10:00 PM (5 months, 16 days ago) |
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Thanks for the write up. I am looking forward to trying glass no-pours. One question - do you re-wrap the individual plates after you do the transfer? I will be using a SAB, if you do re-wrap, are you re-wrapping inside the SAB or after you bring the plate out?
-------------------- “One's destination is never a place, but a new way of seeing things.” - Henry Miller LAGM v. 2.023 LAGM2021
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xVadisx
MountainsDogsAndFungi



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Re: i love glass petris & you can too!! [Re: jay.ach]
#28446801 - 08/25/23 07:41 PM (5 months, 11 hours ago) |
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I have some glass dishes in the PC right now -- excited!
I'll treat them mostly as I do regular plates. Wrap once cooled in bulk. Wrap individually once transferred to. In the SAB/or in flow.
Wrapped up before PC (kind of winged the foil):
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
Edited by xVadisx (08/25/23 08:20 PM)
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NotFromGeorgia
it's 'Mr. Stranger' to you



Registered: 07/18/23
Posts: 327
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Re: i love glass petris & you can too!! [Re: xVadisx]
#28446831 - 08/25/23 08:20 PM (5 months, 10 hours ago) |
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That's is how I've treated my glass dishes and it's worked well so far. Ended up with a lot of condensation but it cleared up in a week-ish.
The cheapo glass dishes feel thin and weak. How sturdy are the nicer pyrex/corning dishes? Worth the cost?
-------------------- The most important step a man can take. It's not the first one, is it? It's the next one. Always the next step, Dalinar.
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xVadisx
MountainsDogsAndFungi



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Re: i love glass petris & you can too!! [Re: NotFromGeorgia]
#28446836 - 08/25/23 08:24 PM (5 months, 10 hours ago) |
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So far they feel great -- solid. They're...glass, but I'm not too worried about them. A different hand-feel than I'm used to. More of a bottom grip with a gentle pull up instead of a one hand side grip as I'd do with plastic. I like them so far! I got the 100x20mm plates for this round. Curious how the 60x15mm feel for later.
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
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NotFromGeorgia
it's 'Mr. Stranger' to you



Registered: 07/18/23
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Re: i love glass petris & you can too!! [Re: xVadisx]
#28446845 - 08/25/23 08:41 PM (5 months, 10 hours ago) |
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That piece of mind alone sounds worth it. The cheaps feel like a hard sneeze is enough to break them. I've also been checking out the 60x15's, kind of annoying that they cost more than the 100s
-------------------- The most important step a man can take. It's not the first one, is it? It's the next one. Always the next step, Dalinar.
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xVadisx
MountainsDogsAndFungi



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Re: i love glass petris & you can too!! [Re: NotFromGeorgia]
#28446854 - 08/25/23 09:03 PM (5 months, 10 hours ago) |
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I saw that -- and that they're back ordered. Maybe I'm reading into nothing but I'm guessing they're the most popular.
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
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xVadisx
MountainsDogsAndFungi



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Re: i love glass petris & you can too!! [Re: xVadisx]
#28447846 - 08/26/23 08:25 PM (4 months, 30 days ago) |
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A quick update as I get into a first time experience with 100x20mm corning pyrex: -poured well mixed agar with lids off and let settle -wrapped in foil, stacked six high (two total stacks) -PCed as normal (not touching water at all) -let cool in the PC for about 30min - an hour -tried to move one of the liquid culture tester agar jars I also PCed -- I didn't wait long enough as the agar moved when jostled -waited overnight -unloaded foil from the PC to a SAB -unwrapped foil in the SAB and set plates to start drying, some had quite a bit of condensation, some had almost none -noticed some water spots? hard water maybe? I'll start using distilled and washing with some vinegar/doing a distilled dunk before re-pouring? -set some plates aside in a separate tote to continue drying and not get in the way -took a few transfers to three glass plates (my first three glass transfers!) and clingwrapped those bitches (note: I like the feel of everything except picking them up -- I feel like I'm going to break them in on themselves -- but they do feel sturdy, just in need of more bottom handling)
I've seen over and over again on the boards problems with condensation. I've had a few problems myself with it. Time seems to be the best answer. Whenever I wanted to use the things right away or try to wrap them too hot (because I wanted them out of my SAB) any plates, glass or not, have had condensation issues. Time. I'm letting the ones that need it chill. Hopefully this works out! I might search for large enough pp5 containers as there would be less exposure risk for the plates as they dry. Anywho. Curious to keep going down the agar path and include now glass in the mix. ...and pre-pours! My first time doing those too.
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
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vazap
Lychen



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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28579403 - 12/11/23 11:06 PM (1 month, 16 days ago) |
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Quote:
like sirpsycho seems to, i actually work barehanded! but if your gloves have good grip that's a huge plus. if you're used to handling glass to that degree, i'm sure you can handle some plates with a deft touch.
i think you'll like em, but i'll take the heat if you don't
What an epic write Hazy, I feel I'll keep coming back to it over and over! So many useful tips, such as using 75mm dishes instead of 90, which often seems overkill for the use I have for them.
The point above is particularly interesting to me, especially when it comes to sealing. I'm fine with gloves as long as I use parafilm, but for anything else (cling, micropore) I find gloves pretty annoying. So I find this re assuring...
Speaking of cling, you mentioned in that other thread using 1.5inch rolls. I couldn't find anything smaller than 5cm, roughly 2", which I feel might cover up too much. So I'm thinking of pre-cutting the roll length wise and see if I can go with 1" only. Making a spiralling down spread if coverage isn't enough. Is this something you've ever heard of?
I've been doing this with micropore rolls today, which seemed practical enough (without gloves!). Can I ask what your thoughts are on micropore as a seal for sensitive plates?
I checked and cling is slightly permeable to vapour and O2 (and CO2), which means it should be fine for mycelial expansion right, not just to store fresh plates? Micropore though seems a lot more porous, so I'm wondering if it's good enough to keep nasties out and prevent drying. Maybe use more layers, like 3 or 4?
--------------------
Reaching for the summit, one way or another
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xVadisx
MountainsDogsAndFungi



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Re: i love glass petris & you can too!! [Re: vazap] 1
#28579607 - 12/12/23 06:45 AM (1 month, 15 days ago) |
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Cling works just fine. I find I don't need it with glass plates but I like using it anyway to keep them from having a lid pop off from my dumbassery or something.
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
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vazap
Lychen



Registered: 10/23/23
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Re: i love glass petris & you can too!! [Re: xVadisx]
#28580537 - 12/12/23 08:51 PM (1 month, 15 days ago) |
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Quote:
xVadisx said: Cling works just fine. I find I don't need it with glass plates but I like using it anyway to keep them from having a lid pop off from my dumbassery or something.
Good to know, thanks. Some people are adamant that cling is only good to wrap sandwiches so I wasn't sure if it was good for inoculated plates..
Edited by vazap (12/12/23 08:53 PM)
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hazyhorse
scoobin



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Re: i love glass petris & you can too!! [Re: vazap]
#28580738 - 12/13/23 03:16 AM (1 month, 15 days ago) |
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Quote:
vazap said:
What an epic write Hazy, I feel I'll keep coming back to it over and over! So many useful tips, such as using 75mm dishes instead of 90, which often seems overkill for the use I have for them.
The point above is particularly interesting to me, especially when it comes to sealing. I'm fine with gloves as long as I use parafilm, but for anything else (cling, micropore) I find gloves pretty annoying. So I find this re assuring...
thanks friend!! yeah the 75mm dishes are perfect. the 90mm are still nice, but they take so much more agar than the 75mm for no practical reason. i had a friend ship me some cultures on pre-sterilizied plastic petris that are 90/100mm & i see where when working with plastic it would be a nice size. but the glass 90mm are pretty bulky in comparison, & when holding a glass 75mm & a plastic 90mm stacked on top of each other they feel pretty close. the 75mm dishes are still smaller but not so much that they feel tiny.
yeah i just don't like throwing a bunch of shit out all the time & i try not to use single use stuff if i can. plus i hate the feel of gloves
Quote:
Speaking of cling, you mentioned in that other thread using 1.5inch rolls. I couldn't find anything smaller than 5cm, roughly 2", which I feel might cover up too much. So I'm thinking of pre-cutting the roll length wise and see if I can go with 1" only. Making a spiralling down spread if coverage isn't enough. Is this something you've ever heard of?
I've been doing this with micropore rolls today, which seemed practical enough (without gloves!). Can I ask what your thoughts are on micropore as a seal for sensitive plates?
oh, i just buy rolls of glad wrap. like this stuff
 (edit: idk why this image is so fucking massive lmao)
then i just cut it into sections at whatever length i want. but you basically just wanna make sure it's enough to make the top & the bottom stay together & cover the gap the lid has. i think micropore works fine, but i've never used it for wrapping plates personally. you really just need to make it easy to handle & not fumble the lid too much while you're handling them. when i wrap them, the lid & bottom are secure & don't move around at all, the lid shouldn't really lift off the rim of the dish imo. but the petri should keep itself sterile just by virtue of how it works (provided the lid stays on & is handled properly)
Quote:
I checked and cling is slightly permeable to vapour and O2 (and CO2), which means it should be fine for mycelial expansion right, not just to store fresh plates? Micropore though seems a lot more porous, so I'm wondering if it's good enough to keep nasties out and prevent drying. Maybe use more layers, like 3 or 4?
by mycelial expansion, do you mean letting it colonize a plate of agar? like taking transfers & stuff? you really don't need much gas exchange (GE) for agar plates. many people use cling wrap just fine on their plates with cultures on them. you definitely don't need 4 layers of micropore tape- that sounds like a huge pain in the ass. i just use a single layer of cling most of the time. if the plates are dry on the outside & if you can get the wrap tight it stays on super well. honestly you could probably just use a little bit of micropore tape on each side to attach the lid to the dish, so it wouldn't even be fully covered on that bottom gap. but i can't guarantee that would work & don't really feel like trying it myself
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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8ctopus
Stranger

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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28581009 - 12/13/23 08:38 AM (1 month, 14 days ago) |
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Just picked up these borosilicate after cracking a plastic dish while wrappinghttps://www.amazon.com/dp/B01MQVUJBS?ref=ppx_yo2ov_dt_b_product_details&th=1 The company is a legit labware co: https://labnique.com/collections/labware They come well reviewed, 10 x 90mm for $30, or 20 x 60mm for $40.
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,654
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Re: i love glass petris & you can too!! [Re: 8ctopus]
#28581269 - 12/13/23 12:58 PM (1 month, 14 days ago) |
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Quote:
8ctopus said: Just picked up these borosilicate after cracking a plastic dish while wrappinghttps://www.amazon.com/dp/B01MQVUJBS?ref=ppx_yo2ov_dt_b_product_details&th=1 The company is a legit labware co: https://labnique.com/collections/labware They come well reviewed, 10 x 90mm for $30, or 20 x 60mm for $40.
Not a bad deal, but I don't see where it says borosilicate. They are likely the thin fragile ones made of soda glass. But that's what I use. They work great, but must be handled like glass.
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vazap
Lychen



Registered: 10/23/23
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28581736 - 12/13/23 06:48 PM (1 month, 14 days ago) |
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Quote:
hazyhorse said:
thanks friend!! yeah the 75mm dishes are perfect. the 90mm are still nice, but they take so much more agar than the 75mm for no practical reason. i had a friend ship me some cultures on pre-sterilizied plastic petris that are 90/100mm & i see where when working with plastic it would be a nice size. but the glass 90mm are pretty bulky in comparison, & when holding a glass 75mm & a plastic 90mm stacked on top of each other they feel pretty close. the 75mm dishes are still smaller but not so much that they feel tiny.
K now I really want to try out 75mm, sounds pretty ideal. Thing is where I live they're hard to find, it's all 60 or 90mm. But I'll keep looking!
Quote:
yeah i just don't like throwing a bunch of shit out all the time & i try not to use single use stuff if i can. plus i hate the feel of gloves
I hear you amigo! Last night I must've kept 'em on too long, my fingers came out super shrivelled. Being a rock climber my finger tips are already pretty fucked up as it is, got a little scared. All back to normal, but yeah I'll keep gloves to a minimum.
Quote:
oh, i just buy rolls of glad wrap. like this stuff
...
then i just cut it into sections at whatever length i want. but you basically just wanna make sure it's enough to make the top & the bottom stay together & cover the gap the lid has. i think micropore works fine, but i've never used it for wrapping plates personally. you really just need to make it easy to handle & not fumble the lid too much while you're handling them. when i wrap them, the lid & bottom are secure & don't move around at all, the lid shouldn't really lift off the rim of the dish imo. but the petri should keep itself sterile just by virtue of how it works (provided the lid stays on & is handled properly)
Yeah I tried cutting cling strips, but again where I live kitchen cling tends to be super flimsy (you guys got better products in the US. Period!)
They pick up so much static it's a nightmare to handle. But then I found this:
https://www.amazon.co.uk/dp/B0B7HDP88D?ref=ppx_yo2ov_dt_b_product_details&th=1
A little on the wide side, but so much better than cutting up the cheap stuff we've got here. It's also a lot thicker, much easier to handle. Thing is it's not food grade, no idea if it's permeable at all, so fingers crossed!
I think I might even look into this tattoo thingy for even more convenience, less wide at 42mm and with a proper dispenser. Luxury!
https://www.amazon.co.uk/Barrier-Disposable-Transparent-Permanent-Accessories/dp/B07XCQ6NNC?th=1
You ever tried one of those?
Quote:
by mycelial expansion, do you mean letting it colonize a plate of agar? like taking transfers & stuff? you really don't need much gas exchange (GE) for agar plates. many people use cling wrap just fine on their plates with cultures on them. you definitely don't need 4 layers of micropore tape- that sounds like a huge pain in the ass. i just use a single layer of cling most of the time. if the plates are dry on the outside & if you can get the wrap tight it stays on super well. honestly you could probably just use a little bit of micropore tape on each side to attach the lid to the dish, so it wouldn't even be fully covered on that bottom gap. but i can't guarantee that would work & don't really feel like trying it myself
Yep that's exactly what I meant, myc colonisation in a plate. Again, that is precious info right there man. So basically the glass dish in itself is sterile by design, and what sealant you use is there primarily to hold it together. Still needs to be contaminant protective in case the lid does lift a little, and keep them from slipping but... I totally get it now. Awesome!
Yeah makes sense, I'll start just getting confident with the technics you give me, then maybe start testing how bold I can get with economy/ease of use..
Good stuff man
--------------------
Reaching for the summit, one way or another
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vazap
Lychen



Registered: 10/23/23
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Re: i love glass petris & you can too!! [Re: vazap]
#28595856 - 12/23/23 10:09 PM (1 month, 4 days ago) |
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Hey I thought I'd give an update and avoid others trying out that thick wrap I mentioned.
The format is convenient, but not the material itself. It doesn't stick to itself very well. But more importantly, it's not permeable enough, I think it's been asphyxiating my plates.
Going back to the classic wrap and micropore. Hey, I tried
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