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SirPsycho
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Re: i love glass petris & you can too!! [Re: Steevil] 1
#28170505 - 02/03/23 09:15 AM (11 months, 17 days ago) |
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Quote:
Steevil said:
Quote:
SirPsycho said:
 Gap and all. Actually "vented" too, if you zoom in at about 4-5 o'clock in the first pic you can see this little plastic line on the lid that keeps a slight gap between it and the bottom.
Yeah the pp5's have a little <1mm gap, and the plastic grips so they don't move at all when you carry a stack.
The glass ones have a 3mm gap - when you first see them it looks like then sent the wrong lid. They rattle around when you move them, it feels like they're drawing in air.
I mean, it's clearly more than 1mm as the spine of my Leatherman fits in there but yes, I do imagine the plastic has higher static friction than glass.
The other thing to consider though is glass plates are generally deeper, 20mm was the most common I found.
Not really sure how to respond to "it feels" like it's drawing in air. Why would it? Is there a pressure difference?
Also, if you read my first post about leaving the dishes unwrapped you'll notice I mentioned I'm doing this at the suggestion of another user, B Traven. I'm pretty sure he's using glass plates.
We can discuss what we think will happen all day and not reach any conclusions. Just try it and see how it goes.
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JHOVA
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Re: i love glass petris & you can too!! [Re: SirPsycho] 1
#28171866 - 02/04/23 10:14 AM (11 months, 16 days ago) |
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SirPsycho
Purple Belt in Google-Fu



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Re: i love glass petris & you can too!! [Re: JHOVA]
#28172032 - 02/04/23 12:48 PM (11 months, 16 days ago) |
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Quote:
JHOVA said:


Damn condensation
Have you tried leaving them unwrapped until use... It'll get rid of the condensation
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LadysKnight
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28172075 - 02/04/23 01:21 PM (11 months, 16 days ago) |
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Hindsight has a method that I haven't tried, but I believe it boils down to insulating them while hot so the agar can absorb the ambient moisture in the plate.
If that works, I wonder if you could just insulate the PC after it's no longer dangerous, for no pour.
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JHOVA
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28172097 - 02/04/23 01:39 PM (11 months, 16 days ago) |
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Quote:
SirPsycho said:
Quote:
JHOVA said:


Damn condensation
Have you tried leaving them unwrapped until use... It'll get rid of the condensation 
next time. i can't spare the time to set them out to dry this round. i have the other 5 in the pp5 container i posted earlier so i'll see if that's enough to dry em out over the next couple days.
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B Traven
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Re: i love glass petris & you can too!! [Re: JHOVA]
#28173072 - 02/05/23 07:58 AM (11 months, 15 days ago) |
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My pyrex dish covers are heavy and the bases have a significant glass bead around the rim. Seems like a pretty solid seal to me. I imagine that a wider cover might actually help reduce turbulence and suction when removing it.
In addition to wrapping my no-pours in foil, I also engage in the antiquated practice of covering my jar lids with foil. Agar plate foil gets repurposed as jar lid foil. I end up with these multi-layer lid hats that eventually start to disintegrate.
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LadysKnight
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Re: i love glass petris & you can too!! [Re: B Traven]
#28173081 - 02/05/23 08:10 AM (11 months, 15 days ago) |
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Quote:
B Traven said: I imagine that a wider cover might actually help reduce turbulence and suction when removing it.
This. I have the cheap glass plates. They are not an exact, uniform product. When a plate has a snug fitting lid, it will not separate easily with one hand, especially when wet. This is why each plate has a dedicated, pre-selected lid with an acceptable gap.
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hazyhorse
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Re: i love glass petris & you can too!! [Re: San Pedro Girl] 1
#28175654 - 02/07/23 01:29 AM (11 months, 14 days ago) |
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took a bit of a break from posting & i'm glad to see this thread is still active!!
Quote:
Stipe-n Cap said: Nice work 
thanks dude! much appreciated.
Quote:
mixomatoz said: Great infos thank you, i had used spawn bags, i clean them up,
then i put some glass petri in them, close them up with an impulse sealer, and i plan to PC them for 1h.
if it worked as intented it will got cleaned and bagged petri good for storage,
thoughts ?
edit: same idea but already with agar in it.?
as long as the bag has a filter on it, i think this would work! so i actually PC'd in an oven bag (used foil to keep them sturdy) & left them on the shelf for about a month and a half. just opened the bag tonight & all the plates are clean! i just made sure to lightly knot the oven bag so that air could escape in the PC, then immediately tightened the knot when i pulled the bag out of the PC. the top agar plates were actually a bit dry, but nothing had contaminated. kinda figured it would be fine, but now i have confirmation. i don't think i'll be storing any plates like this tbh but i could see this being useful if you had an SAB but still wanted to do a bunch of plates all at once.
  
one of the bottom plates took in a bit of water at some point and boiled out into the bag, but other than staining the plastic nothing came of that. you can see that weird dry agar texture in that one plate, but there was no sign of bacteria at all.
Quote:
Steevil said: Great write-up!
Quote:
NuclearTidalWave said: They make autoclaveable containers Like these
I was trying to find one of these for 60mm dishes and came across "stainless steel disinfection jars" (some places call them "cotton swab holders"). They're just stainless steel cans, they're much cheaper than the real deal.
They come in all different diameters and heights so they could fit in a regular PC. I'm guessing they won't seal tight. I've ordered a 65mm x 105mm can, I'll update with how it goes 
thanks! how did these work out? i saw all the PP5 stuff & thought about it, i'll have to try once i have some empty bottles.
Quote:
SirPsycho said: You guys are making this way too hard on yourself and I get it, I used to tape each individual plate with two layers of micropore before pressure cooking.
Just wrapping in foil then removing the foil after the pc and leaving them unwrapped has done great for me. Y'all should give it a shot.
How I wrap and the results after sitting in my drawer for two weeks are a few posts back in this thread.
i'm really shocked they didn't contam, that's some wild news!! i just unwrapped a plate & am gonna see how my glass plates do. mine are the really loose fitting kind from amazon. i'll update if anything happens in the next few weeks.
Quote:
San Pedro Girl said: Or ditch the foil and make it even easier!

i'd love one of these, but i couldn't find any that were short enough to work in my 16qt cooker /:
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Steevil
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28175782 - 02/07/23 06:23 AM (11 months, 13 days ago) |
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Quote:
hazyhorse said:
Quote:
Steevil said: Great write-up!
Quote:
NuclearTidalWave said: They make autoclaveable containers Like these
I was trying to find one of these for 60mm dishes and came across "stainless steel disinfection jars" (some places call them "cotton swab holders"). They're just stainless steel cans, they're much cheaper than the real deal.
They come in all different diameters and heights so they could fit in a regular PC. I'm guessing they won't seal tight. I've ordered a 65mm x 105mm can, I'll update with how it goes 
thanks! how did these work out? i saw all the PP5 stuff & thought about it, i'll have to try once i have some empty bottles.
I'm not sure yet. I got tired of waiting for delivery and bought this tin from a local coffee shop:

After some encouragement with a hammer it snugly fits 5x 60x18mm dishes. I did a dry sterilization test run for 30mins at 180Β°C with no problem, but I'll have to wait and see if dishes contaminate more than with foil. Next time I have empty dishes I'll try a no-pour PC run. I see the proper ones have holes that can align to allow steam in, so I'll drill some of those.
Basically I think I'm going to like using it, but I wouldn't recommend it to anyone else, if you know what I mean. Like if you really can't get a real one or you really want to put it in a PC then get one, but in the end you might still prefer foil.
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San Pedro Girl
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Re: i love glass petris & you can too!! [Re: Steevil]
#28175799 - 02/07/23 06:50 AM (11 months, 13 days ago) |
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Quote:
Steevil said:
Quote:
hazyhorse said:
Quote:
Steevil said: Great write-up!
Quote:
NuclearTidalWave said: They make autoclaveable containers Like these
I was trying to find one of these for 60mm dishes and came across "stainless steel disinfection jars" (some places call them "cotton swab holders"). They're just stainless steel cans, they're much cheaper than the real deal.
They come in all different diameters and heights so they could fit in a regular PC. I'm guessing they won't seal tight. I've ordered a 65mm x 105mm can, I'll update with how it goes 
thanks! how did these work out? i saw all the PP5 stuff & thought about it, i'll have to try once i have some empty bottles.
I'm not sure yet. I got tired of waiting for delivery and bought this tin from a local coffee shop:

After some encouragement with a hammer it snugly fits 5x 60x18mm dishes. I did a dry sterilization test run for 30mins at 180Β°C with no problem, but I'll have to wait and see if dishes contaminate more than with foil. Next time I have empty dishes I'll try a no-pour PC run. I see the proper ones have holes that can align to allow steam in, so I'll drill some of those.
Basically I think I'm going to like using it, but I wouldn't recommend it to anyone else, if you know what I mean. Like if you really can't get a real one or you really want to put it in a PC then get one, but in the end you might still prefer foil.
Drill a small hole straight through the lid and top of the container. When you use it in the PC, it will allow steam to penetrate well. Thatβs one of its main advantages over foil. When you remove it from the PC, just twist the cap 180Β° to offset the holes and seal it.
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JHOVA
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Re: i love glass petris & you can too!! [Re: San Pedro Girl]
#28175823 - 02/07/23 07:14 AM (11 months, 13 days ago) |
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Im still testing with the pp5s. I used a flathead screwdriver which made a tiny hole with tyvek and mp on both the inside and outside. The air flow is far too restricted. For now i removed the outside tape combo.
Next time ill make at least a quarter inch hole for better drying.
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SirPsycho
Purple Belt in Google-Fu



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Re: i love glass petris & you can too!! [Re: hazyhorse] 1
#28175894 - 02/07/23 08:04 AM (11 months, 13 days ago) |
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I was surprised as well but it seems to be consistent so far. Got another set of 24 I did on the 26th and just started using, all look clean. A set I did last week and making more tonight
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Moopers
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Re: i love glass petris & you can too!! [Re: SirPsycho] 1
#28176471 - 02/07/23 04:20 PM (11 months, 13 days ago) |
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I bought a set of cheap glass petri and a set of the pp5 sirpsycho uses.
The cheap glass petris were really hard for me to wrap with parafilm because of the big lid/base gap. On three plates, the parafilm just slid off one side exposing the gap; one now has a little spot of penicillium contam.
They're not smooth/uniform thickness, so the visibility isn't great, kind of like a fun house mirror.
And, the condensation is crazy. I left them 60 hours in the SAB and still had bad pooling. I needed the SAB, so I had to take them out. I inoculated them anyway, which was probably a mistake.
The pp5 seemed to fare better with condensation, not sure why. I used charcoal in that batch. They're also easier to wrap with parafilm.
I'm picking up a set of pyrex 100x15mm and will see if the results are better.
In the meantime, any tips on dealing with the condensation? I used D3 recipe linked in the OP, same brand of agar, with a stick blender. I dried in stacks of 5 in the SAB, but it was a bit cold, like 16-17C.
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San Pedro Girl
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Re: i love glass petris & you can too!! [Re: Moopers]
#28176485 - 02/07/23 04:34 PM (11 months, 13 days ago) |
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Quote:
Moopers said: I bought a set of cheap glass petri and a set of the pp5 sirpsycho uses.
The cheap glass petris were really hard for me to wrap with parafilm because of the big lid/base gap. On three plates, the parafilm just slid off one side exposing the gap; one now has a little spot of penicillium contam.
They're not smooth/uniform thickness, so the visibility isn't great, kind of like a fun house mirror.
And, the condensation is crazy. I left them 60 hours in the SAB and still had bad pooling. I needed the SAB, so I had to take them out. I inoculated them anyway, which was probably a mistake.
The pp5 seemed to fare better with condensation, not sure why. I used charcoal in that batch. They're also easier to wrap with parafilm.
I'm picking up a set of pyrex 100x15mm and will see if the results are better.
In the meantime, any tips on dealing with the condensation? I used D3 recipe linked in the OP, same brand of agar, with a stick blender. I dried in stacks of 5 in the SAB, but it was a bit cold, like 16-17C.
I have Pyrex petris, 100mm x 15mm, and use parafilm M. I pour at 60Β°C and only have condensation issues on the top plate. However, thatβs fixed within a couple days. If you have condensate issues, flip the plate over. If the parafilm slips off the plate, youβre either not stretching it enough or the plate is wet.
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Moopers
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Re: i love glass petris & you can too!! [Re: San Pedro Girl]
#28176505 - 02/07/23 04:52 PM (11 months, 13 days ago) |
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Stretching it over the 3mm gap is the issue, I think. I either pull too hard (it breaks, gets too narrow to cover the gap or just barely covers it) or not hard enough (wide enough to cover gap, not stretched enough to be sticky). Maybe the outsides were still a little wet, too. I'll look out for that next time. Thanks for the tips.
Never had these issues with PS petris, and the pp5 were much easier.
I'm hoping things go better with the Pyrex.
Edited by Moopers (02/07/23 04:55 PM)
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SirPsycho
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Re: i love glass petris & you can too!! [Re: Moopers] 2
#28176576 - 02/07/23 06:05 PM (11 months, 13 days ago) |
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Yes, yes. Embrace the pp, love the pp
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hazyhorse
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Re: i love glass petris & you can too!! [Re: Moopers]
#28177931 - 02/08/23 04:12 PM (11 months, 12 days ago) |
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yo what up moopers!!
for wrapping my petris individually, i use an extra wide cut off a roll of cling wrap. i had considered trying out parafilm but kind of assumed it wouldnβt be wide enough for the gap on the cheap plates because it all looks a lot more narrow than what i use.
iβve gotten lucky with my plates in general, but yeah quality varies really wildly with them. i have one that doesnβt sit flat because the bottom part of the plate has a huge bubble of glass lol. almost all mine are really clear to look through though, so maybe i got lucky there.
very weird about the condensation. if they are wet at all on they outside they are almost impossible to wrap ime. i take it you took them out of the foil to dry? was your SAB totally sealed while they were drying? unless you live in a super humid area i canβt see why it would take so long. maybe cold temps affected that but idk, 60 hours should be plenty of timeβ¦ mine were always good after. a little condensation is pretty hard to avoid but i donβt really care about it as usually they clear up when in storage. only other thing i can think of is if you put the lids on them while the agar was still hot? i let my plates completely cool before adding the lids & stacking for the PC
if i find myself in need of more petris iβll have to try out the pp5s, they sound sick. but iβve had good luck with my glsss so far
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MRB3
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28180160 - 02/09/23 08:45 PM (11 months, 11 days ago) |
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I would just like to add that the name brands are the best for handling. The Pyrex and Klimax both have beaded rims, which make them stronger and more break resistant. Secondly the name brands have a cover that its shorter than the dish which makes handling, opening, and wrapping much easier.
I'll give this no pour another try. I have stainless sleeves that I can PC which has a rack inside to hold them. I did it with the two holes open, so next time, I'll keep those closed so the plates are not so wet. Also, had to modify the base so that my agar was not slanted.
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hazyhorse
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Re: i love glass petris & you can too!! [Re: MRB3] 1
#28180216 - 02/09/23 09:39 PM (11 months, 11 days ago) |
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having a level surface/PC to cool the cooker on is so important with the no pours. iβve had some funky ass slants before i flattened out my PC bottom (im dumb & warped it very early on in my cultivation journey lol)
i would absolutely love some pyrex dishes but thereβs no chance i have the money anytime soon. they look so beautiful though
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ruawakeyet

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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28180442 - 02/10/23 06:09 AM (11 months, 10 days ago) |
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Hazy, I did the same thing with my PCer. What did you use to flatten the bottom back out? I've been thinking about doing the same, but I don't want to damage it.
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