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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
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Re: i love glass petris & you can too!! [Re: eu-da-mon] 3
#28262192 - 04/04/23 02:31 PM (9 months, 19 days ago) |
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Quote:
eu-da-mon said: How are folks labeling their glass petris? China marker seems to be pulled off by the saran wrap on the plate stacked above, and sharpie becomes a vague smear after the first iso spritz.
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Yuggoth
Mi-Go Cultivator


Registered: 03/04/23
Posts: 493
Loc: Lost Carcosa
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Re: i love glass petris & you can too!! [Re: SirPsycho]
#28307862 - 05/05/23 01:57 PM (8 months, 19 days ago) |
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I posted some first impressions of the cheap Scientifics Direct glass petris in the "what new toys did you get" thread.
https://www.shroomery.org/forums/showflat.php/Number/28307857#28307857
tl;dr -- they seem OK but I do not have any of the slightly more expensive Karter petris for comparison.
-------------------- We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 21 minutes, 33 seconds
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Re: i love glass petris & you can too!! [Re: Yuggoth] 4
#28340945 - 05/30/23 06:17 PM (7 months, 25 days ago) |
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gonna update the OP a bit too, but i've been using these bad boys for a few agar runs now & have been happy with em. changed them up a little from my last post about them

there is unfortunately some headspace lost to a slight taper, which leaves me able to do 6 plates at a time when i probably could fit 8 in there otherwise, but it's not a big enough issue for me to work around it yet. i made about 6 holes, may bump it to 8 just to help with drying times, but they seem to dry out well over a few days on the shelf. i also added a thin strip of foil under the bottom petri so i could pick the whole stack up by just pinching the sides of the foil, just makes that bottom plate way easier to handle
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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Edited by hazyhorse (05/30/23 06:28 PM)
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MoJim
Mycobbyist


Registered: 11/23/21
Posts: 29
Loc: PNW
Last seen: 6 hours, 47 minutes
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28371147 - 06/23/23 09:34 AM (7 months, 1 day ago) |
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Ok, I'm convinced. Just ordered "vintage excellent condition" Pyrex USA dishes. 10 for $50 Ebay. Can't wait to try them out.
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Arctic_Fox
Mr. Furious


Registered: 03/13/23
Posts: 257
Loc: Ontario
Last seen: 3 months, 17 days
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Re: i love glass petris & you can too!! [Re: hazyhorse] 2
#28427476 - 08/09/23 06:30 PM (5 months, 16 days ago) |
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I'd like to share a tip for spotless glass petri dishes.
I eliminated water spots on my petri dishes by keeping a container of distilled water like this next to the sink, and dropping them in after washing. Then I take them out, and they are spotless after air drying.

I also use nitrile gloves for washing them to avoid fingerprints.
I found no issues with leaving the water in the container for a week and reusing it for a few rounds of washing 10 or so dishes. If it starts leaving residues or getting soapy, change it.
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jay.ach
Traveler



Registered: 05/25/19
Posts: 531
Loc: USDA Zone 8
Last seen: 2 months, 10 days
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Re: i love glass petris & you can too!! [Re: Arctic_Fox]
#28427684 - 08/09/23 10:00 PM (5 months, 16 days ago) |
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Thanks for the write up. I am looking forward to trying glass no-pours. One question - do you re-wrap the individual plates after you do the transfer? I will be using a SAB, if you do re-wrap, are you re-wrapping inside the SAB or after you bring the plate out?
-------------------- βOne's destination is never a place, but a new way of seeing things.β - Henry Miller LAGM v. 2.023 LAGM2021
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xVadisx
MountainsDogsAndFungi



Registered: 09/03/21
Posts: 1,122
Loc: Secret World HQ
Last seen: 15 hours, 11 minutes
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Re: i love glass petris & you can too!! [Re: jay.ach]
#28446801 - 08/25/23 07:41 PM (5 months, 11 hours ago) |
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I have some glass dishes in the PC right now -- excited!
I'll treat them mostly as I do regular plates. Wrap once cooled in bulk. Wrap individually once transferred to. In the SAB/or in flow.
Wrapped up before PC (kind of winged the foil):
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
Edited by xVadisx (08/25/23 08:20 PM)
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NotFromGeorgia
it's 'Mr. Stranger' to you



Registered: 07/18/23
Posts: 327
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Re: i love glass petris & you can too!! [Re: xVadisx]
#28446831 - 08/25/23 08:20 PM (5 months, 10 hours ago) |
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That's is how I've treated my glass dishes and it's worked well so far. Ended up with a lot of condensation but it cleared up in a week-ish.
The cheapo glass dishes feel thin and weak. How sturdy are the nicer pyrex/corning dishes? Worth the cost?
-------------------- The most important step a man can take. It's not the first one, is it? It's the next one. Always the next step, Dalinar.
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xVadisx
MountainsDogsAndFungi



Registered: 09/03/21
Posts: 1,122
Loc: Secret World HQ
Last seen: 15 hours, 11 minutes
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Re: i love glass petris & you can too!! [Re: NotFromGeorgia]
#28446836 - 08/25/23 08:24 PM (5 months, 10 hours ago) |
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So far they feel great -- solid. They're...glass, but I'm not too worried about them. A different hand-feel than I'm used to. More of a bottom grip with a gentle pull up instead of a one hand side grip as I'd do with plastic. I like them so far! I got the 100x20mm plates for this round. Curious how the 60x15mm feel for later.
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
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NotFromGeorgia
it's 'Mr. Stranger' to you



Registered: 07/18/23
Posts: 327
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Re: i love glass petris & you can too!! [Re: xVadisx]
#28446845 - 08/25/23 08:41 PM (5 months, 10 hours ago) |
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That piece of mind alone sounds worth it. The cheaps feel like a hard sneeze is enough to break them. I've also been checking out the 60x15's, kind of annoying that they cost more than the 100s
-------------------- The most important step a man can take. It's not the first one, is it? It's the next one. Always the next step, Dalinar.
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xVadisx
MountainsDogsAndFungi



Registered: 09/03/21
Posts: 1,122
Loc: Secret World HQ
Last seen: 15 hours, 11 minutes
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Re: i love glass petris & you can too!! [Re: NotFromGeorgia]
#28446854 - 08/25/23 09:03 PM (5 months, 10 hours ago) |
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I saw that -- and that they're back ordered. Maybe I'm reading into nothing but I'm guessing they're the most popular.
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
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xVadisx
MountainsDogsAndFungi



Registered: 09/03/21
Posts: 1,122
Loc: Secret World HQ
Last seen: 15 hours, 11 minutes
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Re: i love glass petris & you can too!! [Re: xVadisx]
#28447846 - 08/26/23 08:25 PM (4 months, 30 days ago) |
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A quick update as I get into a first time experience with 100x20mm corning pyrex: -poured well mixed agar with lids off and let settle -wrapped in foil, stacked six high (two total stacks) -PCed as normal (not touching water at all) -let cool in the PC for about 30min - an hour -tried to move one of the liquid culture tester agar jars I also PCed -- I didn't wait long enough as the agar moved when jostled -waited overnight -unloaded foil from the PC to a SAB -unwrapped foil in the SAB and set plates to start drying, some had quite a bit of condensation, some had almost none -noticed some water spots? hard water maybe? I'll start using distilled and washing with some vinegar/doing a distilled dunk before re-pouring? -set some plates aside in a separate tote to continue drying and not get in the way -took a few transfers to three glass plates (my first three glass transfers!) and clingwrapped those bitches (note: I like the feel of everything except picking them up -- I feel like I'm going to break them in on themselves -- but they do feel sturdy, just in need of more bottom handling)
I've seen over and over again on the boards problems with condensation. I've had a few problems myself with it. Time seems to be the best answer. Whenever I wanted to use the things right away or try to wrap them too hot (because I wanted them out of my SAB) any plates, glass or not, have had condensation issues. Time. I'm letting the ones that need it chill. Hopefully this works out! I might search for large enough pp5 containers as there would be less exposure risk for the plates as they dry. Anywho. Curious to keep going down the agar path and include now glass in the mix. ...and pre-pours! My first time doing those too.
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
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vazap
Lychen



Registered: 10/23/23
Posts: 49
Last seen: 9 hours, 26 minutes
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28579403 - 12/11/23 11:06 PM (1 month, 16 days ago) |
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Quote:
like sirpsycho seems to, i actually work barehanded! but if your gloves have good grip that's a huge plus. if you're used to handling glass to that degree, i'm sure you can handle some plates with a deft touch.
i think you'll like em, but i'll take the heat if you don't
What an epic write Hazy, I feel I'll keep coming back to it over and over! So many useful tips, such as using 75mm dishes instead of 90, which often seems overkill for the use I have for them.
The point above is particularly interesting to me, especially when it comes to sealing. I'm fine with gloves as long as I use parafilm, but for anything else (cling, micropore) I find gloves pretty annoying. So I find this re assuring...
Speaking of cling, you mentioned in that other thread using 1.5inch rolls. I couldn't find anything smaller than 5cm, roughly 2", which I feel might cover up too much. So I'm thinking of pre-cutting the roll length wise and see if I can go with 1" only. Making a spiralling down spread if coverage isn't enough. Is this something you've ever heard of?
I've been doing this with micropore rolls today, which seemed practical enough (without gloves!). Can I ask what your thoughts are on micropore as a seal for sensitive plates?
I checked and cling is slightly permeable to vapour and O2 (and CO2), which means it should be fine for mycelial expansion right, not just to store fresh plates? Micropore though seems a lot more porous, so I'm wondering if it's good enough to keep nasties out and prevent drying. Maybe use more layers, like 3 or 4?
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Reaching for the summit, one way or another
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xVadisx
MountainsDogsAndFungi



Registered: 09/03/21
Posts: 1,122
Loc: Secret World HQ
Last seen: 15 hours, 11 minutes
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Re: i love glass petris & you can too!! [Re: vazap] 1
#28579607 - 12/12/23 06:45 AM (1 month, 15 days ago) |
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Cling works just fine. I find I don't need it with glass plates but I like using it anyway to keep them from having a lid pop off from my dumbassery or something.
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
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vazap
Lychen



Registered: 10/23/23
Posts: 49
Last seen: 9 hours, 26 minutes
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Re: i love glass petris & you can too!! [Re: xVadisx]
#28580537 - 12/12/23 08:51 PM (1 month, 15 days ago) |
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Quote:
xVadisx said: Cling works just fine. I find I don't need it with glass plates but I like using it anyway to keep them from having a lid pop off from my dumbassery or something.
Good to know, thanks. Some people are adamant that cling is only good to wrap sandwiches so I wasn't sure if it was good for inoculated plates..
Edited by vazap (12/12/23 08:53 PM)
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 21 minutes, 33 seconds
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Re: i love glass petris & you can too!! [Re: vazap]
#28580738 - 12/13/23 03:16 AM (1 month, 15 days ago) |
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Quote:
vazap said:
What an epic write Hazy, I feel I'll keep coming back to it over and over! So many useful tips, such as using 75mm dishes instead of 90, which often seems overkill for the use I have for them.
The point above is particularly interesting to me, especially when it comes to sealing. I'm fine with gloves as long as I use parafilm, but for anything else (cling, micropore) I find gloves pretty annoying. So I find this re assuring...
thanks friend!! yeah the 75mm dishes are perfect. the 90mm are still nice, but they take so much more agar than the 75mm for no practical reason. i had a friend ship me some cultures on pre-sterilizied plastic petris that are 90/100mm & i see where when working with plastic it would be a nice size. but the glass 90mm are pretty bulky in comparison, & when holding a glass 75mm & a plastic 90mm stacked on top of each other they feel pretty close. the 75mm dishes are still smaller but not so much that they feel tiny.
yeah i just don't like throwing a bunch of shit out all the time & i try not to use single use stuff if i can. plus i hate the feel of gloves
Quote:
Speaking of cling, you mentioned in that other thread using 1.5inch rolls. I couldn't find anything smaller than 5cm, roughly 2", which I feel might cover up too much. So I'm thinking of pre-cutting the roll length wise and see if I can go with 1" only. Making a spiralling down spread if coverage isn't enough. Is this something you've ever heard of?
I've been doing this with micropore rolls today, which seemed practical enough (without gloves!). Can I ask what your thoughts are on micropore as a seal for sensitive plates?
oh, i just buy rolls of glad wrap. like this stuff
 (edit: idk why this image is so fucking massive lmao)
then i just cut it into sections at whatever length i want. but you basically just wanna make sure it's enough to make the top & the bottom stay together & cover the gap the lid has. i think micropore works fine, but i've never used it for wrapping plates personally. you really just need to make it easy to handle & not fumble the lid too much while you're handling them. when i wrap them, the lid & bottom are secure & don't move around at all, the lid shouldn't really lift off the rim of the dish imo. but the petri should keep itself sterile just by virtue of how it works (provided the lid stays on & is handled properly)
Quote:
I checked and cling is slightly permeable to vapour and O2 (and CO2), which means it should be fine for mycelial expansion right, not just to store fresh plates? Micropore though seems a lot more porous, so I'm wondering if it's good enough to keep nasties out and prevent drying. Maybe use more layers, like 3 or 4?
by mycelial expansion, do you mean letting it colonize a plate of agar? like taking transfers & stuff? you really don't need much gas exchange (GE) for agar plates. many people use cling wrap just fine on their plates with cultures on them. you definitely don't need 4 layers of micropore tape- that sounds like a huge pain in the ass. i just use a single layer of cling most of the time. if the plates are dry on the outside & if you can get the wrap tight it stays on super well. honestly you could probably just use a little bit of micropore tape on each side to attach the lid to the dish, so it wouldn't even be fully covered on that bottom gap. but i can't guarantee that would work & don't really feel like trying it myself
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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8ctopus
Stranger

Registered: 12/04/23
Posts: 1
Last seen: 29 days, 18 hours
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28581009 - 12/13/23 08:38 AM (1 month, 14 days ago) |
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Just picked up these borosilicate after cracking a plastic dish while wrappinghttps://www.amazon.com/dp/B01MQVUJBS?ref=ppx_yo2ov_dt_b_product_details&th=1 The company is a legit labware co: https://labnique.com/collections/labware They come well reviewed, 10 x 90mm for $30, or 20 x 60mm for $40.
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,654
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Re: i love glass petris & you can too!! [Re: 8ctopus]
#28581269 - 12/13/23 12:58 PM (1 month, 14 days ago) |
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Quote:
8ctopus said: Just picked up these borosilicate after cracking a plastic dish while wrappinghttps://www.amazon.com/dp/B01MQVUJBS?ref=ppx_yo2ov_dt_b_product_details&th=1 The company is a legit labware co: https://labnique.com/collections/labware They come well reviewed, 10 x 90mm for $30, or 20 x 60mm for $40.
Not a bad deal, but I don't see where it says borosilicate. They are likely the thin fragile ones made of soda glass. But that's what I use. They work great, but must be handled like glass.
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vazap
Lychen



Registered: 10/23/23
Posts: 49
Last seen: 9 hours, 26 minutes
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Re: i love glass petris & you can too!! [Re: hazyhorse]
#28581736 - 12/13/23 06:48 PM (1 month, 14 days ago) |
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Quote:
hazyhorse said:
thanks friend!! yeah the 75mm dishes are perfect. the 90mm are still nice, but they take so much more agar than the 75mm for no practical reason. i had a friend ship me some cultures on pre-sterilizied plastic petris that are 90/100mm & i see where when working with plastic it would be a nice size. but the glass 90mm are pretty bulky in comparison, & when holding a glass 75mm & a plastic 90mm stacked on top of each other they feel pretty close. the 75mm dishes are still smaller but not so much that they feel tiny.
K now I really want to try out 75mm, sounds pretty ideal. Thing is where I live they're hard to find, it's all 60 or 90mm. But I'll keep looking!
Quote:
yeah i just don't like throwing a bunch of shit out all the time & i try not to use single use stuff if i can. plus i hate the feel of gloves
I hear you amigo! Last night I must've kept 'em on too long, my fingers came out super shrivelled. Being a rock climber my finger tips are already pretty fucked up as it is, got a little scared. All back to normal, but yeah I'll keep gloves to a minimum.
Quote:
oh, i just buy rolls of glad wrap. like this stuff
...
then i just cut it into sections at whatever length i want. but you basically just wanna make sure it's enough to make the top & the bottom stay together & cover the gap the lid has. i think micropore works fine, but i've never used it for wrapping plates personally. you really just need to make it easy to handle & not fumble the lid too much while you're handling them. when i wrap them, the lid & bottom are secure & don't move around at all, the lid shouldn't really lift off the rim of the dish imo. but the petri should keep itself sterile just by virtue of how it works (provided the lid stays on & is handled properly)
Yeah I tried cutting cling strips, but again where I live kitchen cling tends to be super flimsy (you guys got better products in the US. Period!)
They pick up so much static it's a nightmare to handle. But then I found this:
https://www.amazon.co.uk/dp/B0B7HDP88D?ref=ppx_yo2ov_dt_b_product_details&th=1
A little on the wide side, but so much better than cutting up the cheap stuff we've got here. It's also a lot thicker, much easier to handle. Thing is it's not food grade, no idea if it's permeable at all, so fingers crossed!
I think I might even look into this tattoo thingy for even more convenience, less wide at 42mm and with a proper dispenser. Luxury!
https://www.amazon.co.uk/Barrier-Disposable-Transparent-Permanent-Accessories/dp/B07XCQ6NNC?th=1
You ever tried one of those?
Quote:
by mycelial expansion, do you mean letting it colonize a plate of agar? like taking transfers & stuff? you really don't need much gas exchange (GE) for agar plates. many people use cling wrap just fine on their plates with cultures on them. you definitely don't need 4 layers of micropore tape- that sounds like a huge pain in the ass. i just use a single layer of cling most of the time. if the plates are dry on the outside & if you can get the wrap tight it stays on super well. honestly you could probably just use a little bit of micropore tape on each side to attach the lid to the dish, so it wouldn't even be fully covered on that bottom gap. but i can't guarantee that would work & don't really feel like trying it myself
Yep that's exactly what I meant, myc colonisation in a plate. Again, that is precious info right there man. So basically the glass dish in itself is sterile by design, and what sealant you use is there primarily to hold it together. Still needs to be contaminant protective in case the lid does lift a little, and keep them from slipping but... I totally get it now. Awesome!
Yeah makes sense, I'll start just getting confident with the technics you give me, then maybe start testing how bold I can get with economy/ease of use..
Good stuff man
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Reaching for the summit, one way or another
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vazap
Lychen



Registered: 10/23/23
Posts: 49
Last seen: 9 hours, 26 minutes
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Re: i love glass petris & you can too!! [Re: vazap]
#28595856 - 12/23/23 10:09 PM (1 month, 4 days ago) |
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Hey I thought I'd give an update and avoid others trying out that thick wrap I mentioned.
The format is convenient, but not the material itself. It doesn't stick to itself very well. But more importantly, it's not permeable enough, I think it's been asphyxiating my plates.
Going back to the classic wrap and micropore. Hey, I tried
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