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InvisibleSuckatshrooms
Seriously, don’t listen to me.
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Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
Re: i love glass petris & you can too!! [Re: Rusty2096] * 2
    #28018807 - 10/27/22 07:38 AM (1 year, 2 months ago)

I think it’s important to note that your current observations on glass being more fragile may just be brand related. I have Pyrex and noticed the opposite. They are way tougher and less fragile than the plastic. I’ve broken plenty of plastic on accident just trying to wrap, but no issue with the glass.


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InvisibleSuckatshrooms
Seriously, don’t listen to me.
Female

Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
Re: i love glass petris & you can too!! [Re: SirPsycho] * 2
    #28018813 - 10/27/22 07:42 AM (1 year, 2 months ago)

Quote:

SirPsycho said:
What would happen if I left them in the foil until use? One of the main reasons I chose no-pour to start with is not having to set up the SAB timed to the PC cycle and then leaving it out while they cool.



That’s what I do. They come out of the foil in the SAB right before I use them.


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InvisibleSuckatshrooms
Seriously, don’t listen to me.
Female

Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
Re: i love glass petris & you can too!! [Re: SirPsycho] * 1
    #28021497 - 10/28/22 02:09 PM (1 year, 2 months ago)

Quote:

SirPsycho said:
Quote:

Suckatshrooms said:
Quote:

SirPsycho said:
What would happen if I left them in the foil until use? One of the main reasons I chose no-pour to start with is not having to set up the SAB timed to the PC cycle and then leaving it out while they cool.



That’s what I do. They come out of the foil in the SAB right before I use them.



And just to double check, you let them sit in the foil for extended periods of time. I.E. days



I make a bunch at once and save them for weeks. Pour the plates, let solidify, wrap stacks of 4 in foil, PC at 1bar for 20min, let cool to room temp overnight, put in zip lock bag until needed.


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InvisibleSuckatshrooms
Seriously, don’t listen to me.
Female

Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
Re: i love glass petris & you can too!! [Re: bongoman] * 1
    #28028407 - 11/01/22 06:28 PM (1 year, 2 months ago)

Quote:

bongoman said:
And when you wrap them, are you using parafilm or grafting tape?

I'm finding the extra weight of the base and the lid puts more stress on the wrap — I find I need to be a bit more careful handling them so that the wrap doesn't work loose.



I use Parafilm. I haven’t had that problem, but once they’re wrapped, I don’t really handle them much so 🤷


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InvisibleSuckatshrooms
Seriously, don’t listen to me.
Female

Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
Re: i love glass petris & you can too!! [Re: SpreadTheSpore] * 1
    #28029213 - 11/02/22 09:59 AM (1 year, 2 months ago)

Quote:

SpreadTheSpore said:
Sounds like a nice addition.

To be sure:
- You don’t do anything to the dishes to get them dry? Reading that they could be quite wet when taken uit of the PC
- When you take them out of the PC, wrapped in aluminium foil. Do you put them in the ziplock with the aluminium foil still wrapped?

*not sure if I could ask these questions in this journal.. hope it is alright.



When the PC gets back to room temp, I do one of two things.

1) If they’re being stored for a long time, I take them out and straight into zip lock bags, foil and all and straight into the lab refrigerator at 5°C

2) If they’re being used soon, I just put them in my SAB.

They always have some condensation, but usually nothing major. I just ignore it and use as normal. After a week the condensation disappears on its own. Sometimes for unknown reasons, I’ll have one with a pool of water in the bottom. With these, I still just use as normal, but in the SAB, I just pour the excess water out right before I do my transfer or whatever. After I Parafilm wrap it, I usually sanitize the outside with IPA just because I get nervous about any nutrient water from pouring it out sticking to the side and letting bacteria migrate up past the Parafilm.

Pre pour has never made plates as immediately perfect as normal, but for the ease of so few steps, I love it.


Edited by Suckatshrooms (11/02/22 10:00 AM)


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InvisibleSuckatshrooms
Seriously, don’t listen to me.
Female

Registered: 09/26/22
Posts: 157
Loc: Tamaulipas, Mexico
Re: i love glass petris & you can too!! [Re: SpreadTheSpore] * 3
    #28029219 - 11/02/22 10:03 AM (1 year, 2 months ago)

Here’s a pic I just took this morning. It’s dated 22OCT, and you can see there’s still some condensation.


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