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InvisiblePickle Rick
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Registered: 07/24/22
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1/2 jars of LC failed
    #28004823 - 10/18/22 02:37 PM (2 years, 2 months ago)



2 jars of LC made at the same time.
One took really well, one didn’t take at all.
Other than contamination, what causes LC’s to fail?


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Offlinecozmyc
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Re: 1/2 jars of LC failed [Re: Pickle Rick]
    #28004843 - 10/18/22 02:51 PM (2 years, 2 months ago)

If you over sterilize they won't take very well. They take on a darkened hue tho. Looks bacterial


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OfflineSo Anyway
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Re: 1/2 jars of LC failed [Re: cozmyc]
    #28004857 - 10/18/22 02:59 PM (2 years, 2 months ago)

Both look suspect to me, the myc should be more floaty in my experience. But maybe it's ok. Did you inject through the port? Probably introduced contamination there.. what did you shoot in it?


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InvisiblePickle Rick
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Re: 1/2 jars of LC failed [Re: cozmyc]
    #28004873 - 10/18/22 03:07 PM (2 years, 2 months ago)

Quote:

cozmyc said:
If you over sterilize they won't take very well. They take on a darkened hue tho. Looks bacterial




In all (LME) LC's I've made there's been some general cloudiness and sediment after PCing (16 mins @15 psi), which clears up once the mycelium grows and munches on it (like the . All the LC's I made which took, I tested on agar and came out clean, though I haven't tested the one in the photo yet. There have been 3, including the one in the photo that did not take at all. Wondering what the reason could be considering I make them in the exact same way every time.

Quote:

So Anyway said:
Both look suspect to me, the myc should be more floaty in my experience. But maybe it's ok. Did you inject through the port? Probably introduced contamination there.. what did you shoot in it?




They were inoculated at the same time from agar in a SAB. Haven't checked the one that took yet, but it looks the same as all my other LC's which have come out clean.


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InvisiblePickle Rick
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Re: 1/2 jars of LC failed [Re: Pickle Rick]
    #28004883 - 10/18/22 03:14 PM (2 years, 2 months ago)



These, for example have been tested on agar.
No contam, looks the same as the one the took, above


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InvisiblePickle Rick
Gherkin
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Re: 1/2 jars of LC failed [Re: Pickle Rick]
    #28012088 - 10/23/22 08:07 AM (2 years, 2 months ago)

Anyone else have any wise words?


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