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OfflineNaturegroup
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Finding good genetics from spores * 1
    #28004372 - 10/18/22 08:50 AM (2 years, 2 months ago)

After some reading I’ve come across 2 different methods to find good genetics from a spore print but still don’t know which one works best:



Method 1:

1. Streak agar with spores

2. don't bother transferring or isolating if you have clean growth

3. inoculate grain with this agar (the agar will have many different strains on it)

4. Once the grain is fully colonized add it to substrate. Use a LOW grain:sub ratio between 1:10 and 1:6 to help find the strains that grow best on your specific substrate.

5. The grow will be disappointing in that the fruits will be small and your yield will be crap. Nevertheless, clone each fruit making sure to label and store them, then test each clone to see how they produce.

6. store and slant the winning clone.



Method 2:

1. Streak spores on a plate and let them germinate.

2. Transfer clean germination.

3. Keep growing the culture, choose healthy-looking growth (rhizo, tomentose- doesn't matter just clean healthy looking growth) and keep making transfers.

4. After around 8 to 10 transfers you will start to see obvious sectoring.

5. Make a transfer from each individual sector you can see. Transfer from as many as you can see.

6. Grow out these sector transfers. If you are lucky, you will already have some monocultures/isolates

7. Any cultures that is still show sectoring, continue to make transfers until those also show no more sectors and are monocultures.

8. Take your isolated monocultures, label each very carefully and store a  in the fridge. Fruit out each isolate and look for any desirable traits - speed, yield, clustering, potency etc.

10. Store and slant the winning isolate.

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OfflineAwestruck
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Re: Finding good genetics from spores [Re: Naturegroup] * 1
    #28004569 - 10/18/22 11:42 AM (2 years, 2 months ago)

How much time, money and energy do you want to put into this?
For my needs, method two is overkill. Method one better suits my needs, although I go 1:4 on the S2B ratio. Take some good clones and then use those for their own grows. I'm more than happy with that setup.

It's a hobby for me, not a job/life. I'd drown in material if I went with method two, but to each their own.


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Repaying my karmic debt to the Shroomery.
Looking for a Pan cambo print.

Edited by Awestruck (10/18/22 11:43 AM)

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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
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Re: Finding good genetics from spores [Re: Awestruck]
    #28004672 - 10/18/22 12:51 PM (2 years, 2 months ago)

Just grow from spores, identify and select fruits that meet your ideal culture requirement (potency, water movement from the sub, solid stipes, grows in clusters, etc), clone that/those fruits, grow the clone, collect spores from the fruits that best exhibit the traits you want, grow from those spores, select a clone that best resembles the traits you want and grow out that clone, collect spores from the best specimens, grow from those spores, and on and on and on. You get the idea. That's the process for producing the best culture but also for isolating traits.

It sounds complicated but it's really easy, just time consuming.

Also who is recommending a 1:10 ratio for clone hunting? A lot of us will do like a shoebox/ziplock bag/BRF cake/top fruit grain jar for clone hunting to save resources and speed up the process


--------------------



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NOT a virgin!

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OfflineNaturegroup
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Re: Finding good genetics from spores [Re: bigfootscreepyuncl]
    #28004686 - 10/18/22 01:02 PM (2 years, 2 months ago)

That all makes sense to me and that’s what I thought too before I read a bunch of stuff about how each time you start from spores you’re essentially starting from scratch, and germinating spores all from 1 mushroom is almost like inbreeding, so chances are high that you will just be making things worse rather than better unless you get lucky. So in short it’s best to just clone a good fruit and store that clone on a slant doing your best to always avoid growing from spores?

Seems like the more I on the topic read the more confused I become

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Offlinehazyhorse
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Re: Finding good genetics from spores [Re: Naturegroup]
    #28004699 - 10/18/22 01:11 PM (2 years, 2 months ago)

just pick a nice fruit from a good cluster in a well performing tub. grow it out. if it’s sucks, toss it & go back to spore. there are plenty of reasons to grow from spore & some people prefer it, each spore is gonna contain a huge set of genetics & you don’t need to “avoid” it unless you really just wanna run a consistent clone culture (which is what many people do while fucking around with multispore side projects). don’t overthink it man


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OfflineNaturegroup
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Re: Finding good genetics from spores [Re: hazyhorse]
    #28004783 - 10/18/22 02:13 PM (2 years, 2 months ago)

Fair enough I guess I am overthinking things, just want to get shit perfect

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Offlinehazyhorse
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Re: Finding good genetics from spores [Re: Naturegroup]
    #28004794 - 10/18/22 02:19 PM (2 years, 2 months ago)

you’re way more likely to fuck shit up while trying to get it “perfect.” it’s important to do research & make sure you understand what you’re doing obviously, but you’re way better off just growing mushrooms rather than splitting hairs over it

also, i’d recommend against searching for a true isolate unless you got a lot of time & other clone cultures on your hands. clones perform much better than most isolates do given the amount of work you can spend to get a true isolate that sucks ass


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Offlinecozmyc
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Re: Finding good genetics from spores [Re: hazyhorse] * 1
    #28004865 - 10/18/22 03:03 PM (2 years, 2 months ago)

I've been following @Fahtster's methods :mushroom2:


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OfflineThe Mycologist
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Re: Finding good genetics from spores [Re: Naturegroup]
    #28004872 - 10/18/22 03:07 PM (2 years, 2 months ago)

Quote:

hazyhorse said:
just pick a nice fruit from a good cluster in a well performing tub. grow it out. if it’s sucks, toss it & go back to spore. there are plenty of reasons to grow from spore & some people prefer it, each spore is gonna contain a huge set of genetics & you don’t need to “avoid” it unless you really just wanna run a consistent clone culture (which is what many people do while fucking around with multispore side projects). don’t overthink it man




:whathesaid:

No one really cares about isolates bro. Just make sure that the growth is clean and you will have more shrooms then you know what to do with.

See the other thread for extra details on why some people go for isolates but imo its not worth it at all.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:

Edited by The Mycologist (10/18/22 03:08 PM)

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