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InvisibleNerex
Strange as angels

Registered: 02/06/22
Posts: 194
First agar run - nasty stuff and doubts * 1
    #28004734 - 10/18/22 01:39 PM (2 years, 2 months ago)

Hello all, I have 3 things to submit to you attention in order to (try to) become a more informed cultivator and perhaps learn as much as possible of this hobby. So for those that are willing to help, here they are:

1) I've inoculated this plate in the 2 points that you can see in the top and in the bottom of the following picture, but what kind of contamination is the one you can see all around them? It seems like a "hairy" white stuff that is taking over the dish. And also, should I just dump this one or could I save it somehow?



2) I've noted that some of my plates, inoculated with the same MS media (and also transferred), have this kind of big and subtle "aura" growth all around the more visible mycelium (left dish) and others just doesn't have such thing (or they do, but very short), like on the right dish. Could this be due to contamination? Or are these 2 plates OK for you?



3) What kind of contamination if the one in the circles? And also, which kind of contamination if the one all around the mycelium in the botton? It has an orange glow and some black dots in it.



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Offlinehazyhorse
scoobin
I'm a teapot User Gallery


Registered: 03/19/19
Posts: 3,822
Last seen: 2 months, 5 days
Re: First agar run - nasty stuff and doubts [Re: Nerex]
    #28004744 - 10/18/22 01:46 PM (2 years, 2 months ago)

that first plate is pretty gnarly but you could definitely try to save some myc by taking a transfer before the whole dish gets overtaken. no idea what contam it might be but it’s not semp myc

those PE plates look fine, it’s pretty common to have that “aura” you’re talking about

dunno what’s going on in the spot the arrow is pointing to in that last pic but the other spots look like bacteria


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InvisibleNerex
Strange as angels

Registered: 02/06/22
Posts: 194
Re: First agar run - nasty stuff and doubts [Re: hazyhorse]
    #28004750 - 10/18/22 01:52 PM (2 years, 2 months ago)

Quote:

hazyhorse said:
that first plate is pretty gnarly but you could definitely try to save some myc by taking a transfer before the whole dish gets overtaken. no idea what contam it might be but it’s not semp myc

those PE plates look fine, it’s pretty common to have that “aura” you’re talking about

dunno what’s going on in the spot the arrow is pointing to in that last pic but the other spots look like bacteria




Yeah, I could try to save it taking a piece of the bottom mycelium and perhaps transfer it on water agar.

About the third plate: looking at some pictures on the internet, it may be yeast but I'm definitely not sure about that.


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Offlinehazyhorse
scoobin
I'm a teapot User Gallery


Registered: 03/19/19
Posts: 3,822
Last seen: 2 months, 5 days
Re: First agar run - nasty stuff and doubts [Re: Nerex]
    #28004757 - 10/18/22 01:56 PM (2 years, 2 months ago)

water agar would probably be a good idea! haven’t used it myself but it seems like an excellent tool for cleaning up cultures

i’ve only seen yeast in jars but it’s very possible. im by no means a contam ID expert but i know when it’s not cube myc lol


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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InvisibleNerex
Strange as angels

Registered: 02/06/22
Posts: 194
Re: First agar run - nasty stuff and doubts [Re: Nerex]
    #28005192 - 10/18/22 05:49 PM (2 years, 2 months ago)

Hey, thanks for the inputs hazyhorse.

If someone has something to say, it'd be welcome.


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OfflinePDS88
Stranger
I'm a teapot
Registered: 09/30/22
Posts: 33
Last seen: 1 year, 10 months
Re: First agar run - nasty stuff and doubts [Re: Nerex]
    #28005293 - 10/18/22 06:31 PM (2 years, 2 months ago)

Search about a needle biopsy. If for some reason a person really needed to save a culture, a needle biopsy is an option that minimizes transfer of unwanted stuff and it’s quick. Water agar could be used in conjunction.

If I were personally doing xfers from contaminated plates I would do only those and then work with healthy plates in a totally different session to minimize contaminating your clean plates.

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Invisiblebakedbeings
orbiter of truth
 User Gallery

Registered: 09/01/20
Posts: 4,563
Trusted Cultivator
Re: First agar run - nasty stuff and doubts [Re: PDS88]
    #28005467 - 10/18/22 08:21 PM (2 years, 2 months ago)

Quote:

If I were personally doing xfers from contaminated plates I would do only those and then work with healthy plates in a totally different session to minimize contaminating your clean plates.



:justno:

water agar is great. for added style points poke the best part of the plate and squirt it onto a BRF puck, grow that out and transfer chunks to water agar. contam is no match
:rollsafe:


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