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Offlinesenatedestroyer
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P. azurenscens mycelial help
    #28003932 - 10/17/22 09:25 PM (2 years, 2 months ago)

Fist time caller here. I'm working with agar culture for the first time... at least for mycological purposes outside of the lab. Do these look in line for mycelial growth for P. azurenscens? I've seen that it should be pale white and cottony in appearance.... which these three are, but I'd really hate to get a grain inoculation going only to find out I've been growing some rando contaminant with hopes of getting something better. Thanks in advance y'all!!

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OfflineLand TroutM
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Re: P. azurenscens mycelial help [Re: senatedestroyer]
    #28004005 - 10/17/22 10:49 PM (2 years, 2 months ago)

I would keep making transfers.  Looks like there’s some good stuff in there.

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Re: P. azurenscens mycelial help [Re: Land Trout]
    #28004012 - 10/17/22 11:03 PM (2 years, 2 months ago)

Phew! Thanks for that.. i've been at a loss for finding a solid photo of some specifically MYA liquid culture grown. I can't tell if people are afraid to post them or its just not that common of an approach.

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Re: P. azurenscens mycelial help [Re: Land Trout]
    #28006087 - 10/19/22 09:02 AM (2 years, 2 months ago)

Okay so I was hoping for further clarification. What consistency should the myc have on touch? I've done a good deal of digging and have seen that the only real possible contaminant that could be mistaken for the lovely velvet mat would be cobweb mold... which only one plate seems to have grown (just on the control, along with some yeasts and molds). Are there others that can be mistaken for the azures myc or is that pretty much the extent of it?

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OfflineLand TroutM
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Re: P. azurenscens mycelial help [Re: senatedestroyer]
    #28006109 - 10/19/22 09:22 AM (2 years, 2 months ago)



Here’s a couple pics.  The top two are two different azzies.  The third pic is Ps. Cyanescens.  The myc can vary from flat and dense to fine, rizomorphic isn’t very common on agar, but it can show.  The myc can also change, a clone I took was very ropy till the second transfer and turned to a finer wispier myc on agar, then gets much more robust once it gets back on wood. 
As far as molds go they will be much more fragile, and a lot more puffier/taller, more like clouds.  If you can share some better pics of your plates please.  It looks like in the first pic the myc is being repelled by something,  the plate looks clear, but how it’s forming an arch is common when there’s a culture of bacteria or something near by.  How fast is the myc growing? mold myc will outrun mushroom myc, typically, and then blow spores all over the plate, so it’s nice to make transfers quickly once you can tell you’ve got what.
Are you pretty new to growing fungi?  The perennial wood eaters are real tests of patience, easy to pull off in the right conditions, but they take a lot of time.  They are very rewarding.  Takes some faith tho.  I always suggest make a lot and plant them in a lot of places, especially places you can easily control.  It’s pretty discouraging to wait a year or two to see the thing die before getting a fruit from it.
I’d recommend jumping over to this thread, and look through the threads in my sig for some more info.
Woodlovers thread

Edited by Land Trout (10/19/22 09:37 AM)

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Offlinesenatedestroyer
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Re: P. azurenscens mycelial help [Re: Land Trout]
    #28006142 - 10/19/22 09:46 AM (2 years, 2 months ago)

I see. I'm new to agar cultivation but have done a decent amount with grain spawn from LC or SS. All but one of my grows have been outdoor beds... and some random dispersal throughout my properties forest. I have no illusions of attempting these fellas indoors with the expectation of success. I'll post some further photos later today of some of the plates i have. I split my medium up on my plates as well, MYA and PDA, just to fuck around and find out. I've gotten the growth under magnification (up to 40x... no way to easily use immersion oil with it and get the right focal plane) and observed what appear to by hyphae. There don't seem to be ANY other structures visible... but I think I'm thrown off because the growth doesn't appear to be organized in any particular way. Every photo and description I have found has appeared to be of a tissue sample, or what i assume are from LC/SS placed by someone with greater care than I have (thus far).

I think the biggest thing throwing me off is that all but a few have contamination from my poor choice in inoculation space. Old houses FOR SURE require a laminar hood (or still air box). So then i get to thinking: "okay, if i have contamination already it would make sense the the ones that look like myc growth are just a different form of microbial intrusion that i just cant find photos for."
I was thinking that since i had a few drops that ended up traversing the plate my growth wouldn't really be radiating from any point but would look pretty archaic. Maybe I'm just making assumptions here...

Thanks for the feedback and info as usual! All is appreciated.

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Offlinesenatedestroyer
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Re: P. azurenscens mycelial help [Re: senatedestroyer]
    #28014434 - 10/24/22 04:39 PM (2 years, 2 months ago)

https://https://imgur.com/a/M8UQY4c

Here are some clearer images.

:edit: realized in my sleep stupor last night that I tried to embed the link as an image :rasta:

Edited by senatedestroyer (10/25/22 11:05 AM)

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