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Offlineimushgrowmushroom
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How many CC's of liquid culture to use per 1L grains
    #28002815 - 10/17/22 08:36 AM (2 years, 2 months ago)

Hi,

How many CC's or ml of liquid culture per 1liter of grains?

I'm seeing mixed results. My plan is to do 5cc's per 1Liter grains. Will this be in order?

Thanks.

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OfflineJacubey
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Re: How many CC's of liquid culture to use per 1L grains [Re: imushgrowmushroom]
    #28002825 - 10/17/22 08:42 AM (2 years, 2 months ago)

I usually use about 1ml. Using more makes the grains too wet in my experience


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Invisiblebakedbeings
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Re: How many CC's of liquid culture to use per 1L grains [Re: Jacubey] * 1
    #28002950 - 10/17/22 10:40 AM (2 years, 2 months ago)

youll find conflicting info because theres no right answer really. 1cc is enough. if your grains are well prepped, sterile and dry, and your LC is well colonized you can be more liberal with it. i pour it straight from the jar and i eyeball a tablespoon (15cc). but thats because i trust my prep and i always have more LC than i know what to do with


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Offlineimushgrowmushroom
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Re: How many CC's of liquid culture to use per 1L grains [Re: bakedbeings]
    #28002970 - 10/17/22 10:56 AM (2 years, 2 months ago)

Awesome feedback, thanks!

I inoculated tonight, one of my LC's looked a bit cloudy and "spermy" comparing it to the other one that is clear. But it's really not that much?

Anything to worry about?

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Invisiblebakedbeings
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Re: How many CC's of liquid culture to use per 1L grains [Re: imushgrowmushroom]
    #28002984 - 10/17/22 11:08 AM (2 years, 2 months ago)

too late to worry about it now, time will tell


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Confused? Well now you can!
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OfflineJaksavage
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Re: How many CC's of liquid culture to use per 1L grains [Re: imushgrowmushroom]
    #28003007 - 10/17/22 11:28 AM (2 years, 2 months ago)

Welcome to the Shroomery!

:goodluck: with :sporedance:


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InvisiblefahtsterM
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Re: How many CC's of liquid culture to use per 1L grains [Re: Jaksavage] * 2
    #28003082 - 10/17/22 12:53 PM (2 years, 2 months ago)

I usually do 5ml lc and there’s no reason to let it all sit on the bottom of the jar..  after you inoculate, turn the jar on its side and slowly roll it and watch the lc dissipate as it gets stuck to the kernels on the side of grain column.  By the 2nd or third rotation, there’s no lc left to collect on the bottom.  Then you end up with lots of growth all over the outside of the grain column


The most recent example of doing this is, this jar that I used ~6ml of lc on the 11th and by day 3 after the inoculation, the growth was barely there but because there was so much of it, I shook it and it’ll be done tomorrow

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Offlineimushgrowmushroom
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Re: How many CC's of liquid culture to use per 1L grains [Re: fahtster]
    #28004030 - 10/17/22 11:38 PM (2 years, 2 months ago)

Thanks, I tried spreading it around last night. I bought grains online and they came in plastic containers. I'm scared to leave it on its side due to possible leaking. I'm very excited and this is my first time!! I'll post an update in a couple of days.

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InvisiblefahtsterM
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Re: How many CC's of liquid culture to use per 1L grains [Re: imushgrowmushroom]
    #28004045 - 10/17/22 11:59 PM (2 years, 2 months ago)

Well with the jars I sit back up straight after dispersing the lc.  I’m sure it’ll be fine either way

I’m excited for you :thumbup:

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