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Sporical
Gonzo journalist


Registered: 08/27/22
Posts: 46
Loc: Up in the clouds
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First monos bacterial/ blob please help updated 10/23 more pics
#28002528 - 10/17/22 12:21 AM (2 years, 2 months ago) |
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I have 3 monos, two MS agar>grain and one isolate. I went to bulk on 10/1 and have let them sit for 2 weeks. hoping to check in and see pins/ fruit. I discovered some issues and have since increased my FAE, but now fear I may Have found the onset of cobweb mold in one tub and fighting bacteria in another. Here is there first tub MS Golden Halo suspected cob web mold.

 10/23 update I got a blob, and some pins.thoughts?

Couple more fruits but one cracked? Tub looks too moist too me though. Probably stalled? Blob has grown the most in last 2-3 days.

Here is the other MS PB just started getting yellowing on the surface myc, 2 days ago.


10/23 Update it's pinning but many are covered in yellow. Fine to harvest? If they make it. Anyone clean before dehydration? If so how? Light scrub under cold water?

Should I get a bubble tub?
 
And my third tub witch is serving well as a control right now. π Is the isolate WR knotting up well but obviously I'm new I could miss something.

10/23 Update WR taking off

Any and all help is appreciated. I have photo doc from the syringe of all 3 tubs.
-------------------- Buy the ticket take the ride.
H.S.T.
Edited by Sporical (10/23/22 03:58 PM)
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Vinci
I'm You



Registered: 08/26/19
Posts: 649
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Re: First monos possible cobweb mold/ bacterial please help [Re: Sporical]
#28002544 - 10/17/22 12:45 AM (2 years, 2 months ago) |
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Contamination issues all almost 100% of the time improper sterile work or improper grain prep.
-------------------- Vinci luh you
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Sporical
Gonzo journalist


Registered: 08/27/22
Posts: 46
Loc: Up in the clouds
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Re: First monos possible cobweb mold/ bacterial please help [Re: Vinci]
#28002558 - 10/17/22 01:03 AM (2 years, 2 months ago) |
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Agreed this is why I'm not surprised it's my two MS tubs though I had higher hopes. I'm trying to learn as everything appeared to be ok until I opened the tubs 2 days ago. Can you identify the potential contamination?
-------------------- Buy the ticket take the ride.
H.S.T.
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MagicMan90
Stranger


Registered: 10/13/22
Posts: 13
Loc: Land of Ooo
Last seen: 1 year, 9 months
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Re: First monos possible cobweb mold/ bacterial please help [Re: Sporical]
#28003411 - 10/17/22 04:53 PM (2 years, 2 months ago) |
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Bit hard to see from the photos. If your talking about the off white spots in pic one that looks like incoming primordia to me. The 2nd pic looks a little gooey and surface conditions a bit moist. Id increase FAE and not mist unit it evaporates a bit and see what happens. Good luck out there.
Edited by MagicMan90 (10/17/22 04:58 PM)
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Sporical
Gonzo journalist


Registered: 08/27/22
Posts: 46
Loc: Up in the clouds
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Re: First monos possible cobweb mold/ bacterial please help [Re: MagicMan90]
#28004000 - 10/17/22 10:42 PM (2 years, 2 months ago) |
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Thanks for the input. Just noob needing some validation I guess. I have not misted as they as monos but have since flipped the lids and have started seeing pins in the GH tub!keeping a close eye on FAE and off color spots. Unfortunately yellowing in my PB tub as increased dramatically. I am now worried if it's salvageable. I have put it in isolation. Thoughts? On the verge of preemptively tossing just to keep my areas clean.   
-------------------- Buy the ticket take the ride.
H.S.T.
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,822
Last seen: 2 months, 5 days
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Re: First monos possible cobweb mold/ bacterial please help [Re: Sporical]
#28004002 - 10/17/22 10:45 PM (2 years, 2 months ago) |
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donβt toss it unless you see a lot of color pop up. it may be bacterial but iβd let that tub ride longer. if you see green pop up toss it but youβre not shit out of luck on it yet
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Sporical
Gonzo journalist



Registered: 08/27/22
Posts: 46
Loc: Up in the clouds
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Re: First monos possible cobweb mold/ bacterial please help [Re: hazyhorse]
#28012796 - 10/23/22 04:02 PM (2 years, 2 months ago) |
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Right on thanks for the input.
-------------------- Buy the ticket take the ride.
H.S.T.
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