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Offlineourcylium
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Contaminated? should i throw these ones out? Help please
    #28002470 - 10/16/22 10:40 PM (2 years, 2 months ago)

Hello, Newbie here first grow 2 tubs look contaminated. Pretty sure this are a no go, but want confirmation before throwing them out. Thanks for your help.

Tidal Wave2

Grain popcorn
Substrate:

65g Coco coir
350g Vermiculite
50g Gybsum
50g Calcium Hydroxide
.5 Qt Water

Pasteurized in a Bucket. Forgot to mix before putting on the tubs as a result Tub # 1 had too much water and tub # 2 less water.



Tub #1 fully colonized lots of Mycilium piss as you can see no sign of pins at 2 weeks fruiting using micropore tape and lid without the lock.




Tub #2 never fully colonized

Edited by ourcylium (10/17/22 04:29 AM)

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Offlinecozmyc
gentle modern ape
I'm a teapot User Gallery


Registered: 06/20/21
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Re: Contaminated? should i throw this ones out? [Re: ourcylium]
    #28002472 - 10/16/22 10:43 PM (2 years, 2 months ago)

Yipes! Calcium hydroxide is only needed with peat casings. Got anything brewing behind these?


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You're conscious population 2 stardust
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Nothing matters so everything does :sunny:

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Offlinecozmyc
gentle modern ape
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Re: Contaminated? should i throw this ones out? [Re: cozmyc]
    #28002476 - 10/16/22 10:47 PM (2 years, 2 months ago)

Welcome:mushroom2:
Try PF tek to get fruits to clone to agar


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You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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Offlineourcylium
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Re: Contaminated? should i throw this ones out? [Re: cozmyc]
    #28002477 - 10/16/22 10:48 PM (2 years, 2 months ago)

B+ same substrate mix day#3 into colonization

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Offlineourcylium
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Re: Contaminated? should i throw this ones out? [Re: cozmyc]
    #28002480 - 10/16/22 10:51 PM (2 years, 2 months ago)

Thanks, I did put some grains into agar in case my grows got contaminated i could start again. hope this are fine.


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Offlineourcylium
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Re: Contaminated? should i throw this ones out? [Re: ourcylium]
    #28002587 - 10/17/22 02:38 AM (2 years, 2 months ago)

Recap photo of my 4 tubs, are all of them contaminated? Thanks for your help.

Tub #1


Tub#2


Tub #3


Tub#4

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Offlineourcylium
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Re: Contaminated? should i throw this ones out? [Re: ourcylium]
    #28002868 - 10/17/22 09:28 AM (2 years, 2 months ago)

Can somebody help me know if they are contaminated and how to spot it. Thanks

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OfflineHappinessStan
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Re: Contaminated? should i throw this ones out? [Re: ourcylium]
    #28002931 - 10/17/22 10:24 AM (2 years, 2 months ago)

Tub 1 and 2 don't look good at all. 3 and 4 look far more promising.
Bad pics but those agar plates don't look too great. Can you get some individual close ups of each plate? Try experimenting with different angles for lighting so we can see the plates as clearly as possible although from here, it looks like you've got some cleaning to do.
Any pics of the jars you used to spawn these tubs?


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Offlinechris77
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Re: Contaminated? should i throw this ones out? [Re: ourcylium]
    #28002934 - 10/17/22 10:25 AM (2 years, 2 months ago)

tub 3 looking quite good from here


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the observer is the observed
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Invisiblebakedbeings
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Re: Contaminated? should i throw this ones out? [Re: chris77]
    #28002963 - 10/17/22 10:51 AM (2 years, 2 months ago)

i would toss tub 1, keep an eye on tub 2, and wait around for 3 and 4

you definitely need to do some transfers before putting agar to grain


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Offlineourcylium
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Re: Contaminated? should i throw this ones out? [Re: HappinessStan]
    #28003181 - 10/17/22 02:13 PM (2 years, 2 months ago)


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OfflineScrewup
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Re: Contaminated? should i throw this ones out? [Re: ourcylium]
    #28003219 - 10/17/22 02:29 PM (2 years, 2 months ago)

Jars all bacterial and those two bottom agar plates likely got some riding along. Those other ones look better IMO.

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Offlineourcylium
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Re: Contaminated? should i throw this ones out? [Re: ourcylium]
    #28003311 - 10/17/22 03:25 PM (2 years, 2 months ago)

Thank you all, i will throw away tub 1 and 2. Will wait for 3 and 4 and hopefully they will be ok.  So much work and waiting in vein.  But expected for first grow, from now on only transfers from agar to grain for my spawn.

Thanks to all for your help

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