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OfflineKingCloyster
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Registered: 05/27/13
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Loc: Quetico, Canada
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Did alcohol vapors kill my spore print if sealed in a bag?
    #27997597 - 10/13/22 08:51 PM (2 years, 3 months ago)

So let’s say I have a pan cyan spore print and I’ve gotten it to germinate in 48 hours no problem. Next time I go to use it on some new agar plates, I get no germination at 2+ weeks. I’ve made 3 plates with no germination and I know it’s not the heat.

I’m wondering if I maybe didn’t let the alcohol dry off the foil encasing the spore print and sealed it in a bag with it... would that ruin the entire spore print from the vapors in the bag?

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OfflineKingCloyster
Forest Mushroom 🍄
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Registered: 05/27/13
Posts: 597
Loc: Quetico, Canada
Last seen: 2 years, 5 days
Re: Did alcohol vapors kill my spore print if sealed in a bag? [Re: KingCloyster]
    #28013779 - 10/24/22 09:09 AM (2 years, 2 months ago)

The answer is yes, alcohol vapors can kill a spore print. I got a new print, same species on the same plates that didn’t germinate, and the new swipe germinated in two days.

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