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OfflineTrickymush
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How’s my 2nd tub looking? *DELETED*
    #27996884 - 10/13/22 01:37 PM (2 years, 3 months ago)




Edited by Trickymush (10/14/22 06:25 AM)

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InvisibleGuerrilla
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Re: How’s my 2nd tub looking? [Re: Trickymush] * 1
    #27996909 - 10/13/22 01:57 PM (2 years, 3 months ago)

Horribly contaminated beyond any point of saving


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OfflineScrewup
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Re: How’s my 2nd tub looking? [Re: Guerrilla] * 1
    #27996910 - 10/13/22 01:58 PM (2 years, 3 months ago)

Lmao that shit looks like the mold I get on my agar plates goddamn.

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OfflineTrickymush
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Re: How’s my 2nd tub looking? [Re: Screwup]
    #27996926 - 10/13/22 02:10 PM (2 years, 3 months ago)

Holy shit lol wait where do you see mold so I know for future? I separated it just in case bc it was on top my first tub that got trich.

It’s funny but I promise I will have a nice tub someday not too far from now. I’ve got two that look better that I didn’t seperate I just wanted to see if this one could fruit. But ok just let me know where you see the bad things. Is it the yellow on sides?

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OfflineScrewup
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Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27996931 - 10/13/22 02:14 PM (2 years, 3 months ago)

??? The entire second photo is mold lol

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InvisibleGuerrilla
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Re: How’s my 2nd tub looking? [Re: Screwup]
    #27996937 - 10/13/22 02:15 PM (2 years, 3 months ago)

Yeah it's literally everywhere dude you can't miss it


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OfflineTrickymush
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Re: How’s my 2nd tub looking? [Re: Screwup]
    #27996952 - 10/13/22 02:25 PM (2 years, 3 months ago)

Ok well I’m new here. I didn’t know mold could be yellow. Honestly I thought it was metabolites bc mycelium was stressed instead of mold. Now it looks like my 3rd tub has that shit. Is lack of a liner causing that?

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OfflineScrewup
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Re: How’s my 2nd tub looking? [Re: Trickymush] * 1
    #27996960 - 10/13/22 02:30 PM (2 years, 3 months ago)

Lol lack of clean spawn is causing that. How are you inoculating your spawn and what tek are you (not) following?

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OfflineTrickymush
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Re: How’s my 2nd tub looking? [Re: Screwup]
    #27996974 - 10/13/22 02:43 PM (2 years, 3 months ago)

Following bods teks. I did take a shortcut by pcing oat jars for 1 hr 30 min instead of 2 hr so I will go back to 2 hr like I did the first time. Cooking oats now for my g2g I have like 10 master jars to choose from some aren’t active though

I’m going agar to grain spawn to g2g to bulk

Now I will say I haven’t done 3 transfers of agar to spawn so maybe I need to do that. I’ve tried to take shortcut so maybe 2 transfer agar to spawn most times I’ve done first agar plate to spawn

Edited by Trickymush (10/13/22 02:45 PM)

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OfflineScrewup
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Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27996989 - 10/13/22 02:55 PM (2 years, 3 months ago)

Post pics of the agar and master jars.

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InvisibleGuerrilla
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Re: How’s my 2nd tub looking? [Re: Screwup]
    #27996992 - 10/13/22 02:58 PM (2 years, 3 months ago)

:whathesaid:

Document your process and you can avoid fucking up / learn from it


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Invisiblebakedbeings
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Re: How’s my 2nd tub looking? [Re: Guerrilla]
    #27996999 - 10/13/22 03:04 PM (2 years, 3 months ago)

yeah post grain jars before you spawn them. in the beginning its hard to eyeball clean spawn


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Offlinecozmyc
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Re: How’s my 2nd tub looking? [Re: bakedbeings]
    #27997035 - 10/13/22 03:30 PM (2 years, 3 months ago)

Bananas? 🍌


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OfflineTrickymush
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Re: How’s my 2nd tub looking? [Re: cozmyc]
    #27997127 - 10/13/22 04:26 PM (2 years, 3 months ago)

Here’s 1 of 7 active master jars. Tidalwave inoculated 10-6 did a tiger drop for first time just to see how those do. I just shook them all up 2 days ago I will post each jar


Edited by Trickymush (10/13/22 04:28 PM)

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OfflineTrickymush
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Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27997138 - 10/13/22 04:33 PM (2 years, 3 months ago)

Here’s AA+ spawned on 10-6 this may have been a single wedge either that or a medium piece chopped up



Here’s Jack Frost spawned on 9-21 single wedge 1st plate from spore swab from a company I even have the pic of plate I took it from with wedge missing here’s that as well


Here’s AA+ spawned 10-6 another tiger drop I heard people on Reddit did these. Let me know if I should stop doing tiger drops


Here’s some new plates I nocked on 10-6




Edited by Trickymush (10/13/22 04:47 PM)

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OfflineScrewup
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Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27997183 - 10/13/22 05:06 PM (2 years, 3 months ago)

All the jars are bacterial to some degree. Not as bad as I though to get that yellow mold but I’m not that experienced. The uncolonized grain’s  pressed against the glass are an indicator.

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OfflineTrickymush
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Re: How’s my 2nd tub looking? [Re: Screwup] * 1
    #27997190 - 10/13/22 05:10 PM (2 years, 3 months ago)

Perhaps from only pcing for 1 hr 30 instead of 2 hr? Also I use the same polyfil in lids should I replace those each time I start a jar?

Edited by Trickymush (10/13/22 05:15 PM)

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OfflineScrewup
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Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27997193 - 10/13/22 05:11 PM (2 years, 3 months ago)

It could be because of that…at the very least those agar should probably be transferred another time or two before sending it to go to grain.

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OfflineTrickymush
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Re: How’s my 2nd tub looking? [Re: Screwup]
    #27997197 - 10/13/22 05:16 PM (2 years, 3 months ago)

Ok I’ll start doing more agar work… can u check my edit about the polyfil?

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OfflineScrewup
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Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27997201 - 10/13/22 05:19 PM (2 years, 3 months ago)

If you’re using polyfil on your jars while they’re colonizing grain that’s not good enough to keep contaminates out. You need to use Synthetic filter discs.

Are you dying your agar red? Don’t do that. Red dye has anti fungal properties I think?

Edited by Screwup (10/13/22 05:20 PM)

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OfflineTrickymush
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Re: How’s my 2nd tub looking? [Re: Screwup]
    #27997221 - 10/13/22 05:42 PM (2 years, 3 months ago)

Bod uses polyfil man. I’ve colonized oyster fine with these polyfil lids I will replace them with new polyfil next time I use though just to be safe. I bought an oyster LC and it had 100% success rate when spawn to straw buckets. Bod wouldn’t tell people to use polyfil lids if it didn’t work

I also heard some grain stuck is fine but I would like to hear someone else’s opinion. The grains stick may be contam like ur saying bc the oyster jars did not have grains stuck.

I just read about the red dye last night but those plates were already made. I forgot I had to order more agar agar so I have to wait for that but I will stop using red just in case but I mean most those plates ate those plates up fine.

I’m not saying you’re wrong on everything but I do have to disagree on the polyfil part. And the red dye thing is kind of up in the air I believe. Just in case I’ll stop using red bc it’s not a big issue to not use it. I’ll probably goto SFD after I use my polyfil up which will honestly be a while and it worked 100% with an isolated oyster LC I bought

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Invisiblecooleko
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Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27997265 - 10/13/22 06:06 PM (2 years, 3 months ago)

Quote:

Trickymush said:
Bod uses polyfil man. I’ve colonized oyster fine with these polyfil lids I will replace them with new polyfil next time I use though just to be safe. I bought an oyster LC and it had 100% success rate when spawn to straw buckets. Bod wouldn’t tell people to use polyfil lids if it didn’t work
Oysters grow so fast they outrun a lot of contamination. Cubes dont.

I also heard some grain stuck is fine but I would like to hear someone else’s opinion. The grains stick may be contam like ur saying bc the oyster jars did not have grains stuck.
As he said, indicator. But I agree that every single jar pictured is contaminated. You really need to pc the full time because grains harbor some resilient contams. Under PCed oats are almost a 100% contam rate for me so I learned to stick with 2-3 hours really fast.

I just read about the red dye last night but those plates were already made. I forgot I had to order more agar agar so I have to wait for that but I will stop using red just in case but I mean most those plates ate those plates up fine.

I’m not saying you’re wrong on everything but I do have to disagree on the polyfil part. And the red dye thing is kind of up in the air I believe. Just in case I’ll stop using red bc it’s not a big issue to not use it. I’ll probably goto SFD after I use my polyfil up which will honestly be a while and it worked 100% with an isolated oyster LC I bought



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OfflineTrickymush
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Re: How’s my 2nd tub looking? [Re: cooleko]
    #27997303 - 10/13/22 06:28 PM (2 years, 3 months ago)

Fair enough I hear you both and I will try some SFD jars in the future. I’ll order some now.

Yeah Reddit has some bullshit info sometimes. That’s where I got the 1 hr 30 min time at. Someone from Reddit said just mist the substrate yet YouTube says mist jus the walls and lid lmao ok which one?

I’m going to start transferring agar plates more and also pcing longer and hopefully those two alone will help me a lot. Only replace polyfil if it looked like it needed replacing

Edited by Trickymush (10/14/22 06:29 AM)

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OfflineNayterade
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Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27997365 - 10/13/22 07:00 PM (2 years, 3 months ago)

Quote:

Trickymush said:
Following bods teks. I did take a shortcut by pcing oat jars for 1 hr 30 min instead of 2 hr so I will go back to 2 hr like I did the first time. Cooking oats now for my g2g I have like 10 master jars to choose from some aren’t active though

I’m going agar to grain spawn to g2g to bulk

Now I will say I haven’t done 3 transfers of agar to spawn so maybe I need to do that. I’ve tried to take shortcut so maybe 2 transfer agar to spawn most times I’ve done first agar plate to spawn





Stop taking shortcuts. Follow Teks to the "T" Chances are if you're taking those shortcuts, you're taking others. Are you using an SAB when you're working with agar and doing tiger drops? Maybe go back to basics and get your technique right, then go to more advanced procedures. I am not saying any of this to be a dick, I'm really just saying, shortcuts get you nowhere, when every single jar you have is contaminated like that. Good luck man, you can do it! Just take your time and do it right. FOLLOW THE TEKS.


--------------------

Edited by Nayterade (10/13/22 07:03 PM)

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OfflineTrickymush
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Re: How’s my 2nd tub looking? [Re: Nayterade]
    #27997491 - 10/13/22 08:02 PM (2 years, 3 months ago)

I hear you. The only shortcuts I’ve taken was cutting oat pc time from 2 hr to 1 hr 30 min and I believe it’s been causing contams. Plus other shortcut is not doing 3 transfers from MSS plate

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Offlinecozmyc
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Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27997543 - 10/13/22 08:29 PM (2 years, 3 months ago)

Don't forget to vent. I vent at least 15 min now.


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You're conscious population 2 stardust
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OfflineTrickymush
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Re: How’s my 2nd tub looking? [Re: cozmyc] * 1
    #27997750 - 10/13/22 10:09 PM (2 years, 3 months ago)

Ok I do 10 minutes but fuck it I guess I’ll do 15 since I heard 20 from someone yesterday lol

I appreciate the heads up bc it would’ve been great to hear that if I wasn’t venting. So thanks anyway homie

Edited by Trickymush (10/13/22 10:09 PM)

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Invisiblezmicroinquisitor
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Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27997765 - 10/13/22 10:19 PM (2 years, 3 months ago)

What is this venting you speak of?


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Offlinecozmyc
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Re: How’s my 2nd tub looking? [Re: zmicroinquisitor]
    #27997802 - 10/13/22 10:51 PM (2 years, 3 months ago)

When your PC has a good steady stream of steam coming out and the lock locks, set timer for 15-20min, to insure all trapped air is purged. This let's you reach the temp you need.


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You're conscious population 2 stardust
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Nothing matters so everything does :sunny:

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OfflineHappinessStan
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Re: How’s my 2nd tub looking? [Re: cozmyc]
    #27997974 - 10/14/22 01:44 AM (2 years, 3 months ago)

Quote:

Screwup said:
If you’re using polyfil on your jars while they’re colonizing grain that’s not good enough to keep contaminates out. You need to use Synthetic filter discs.

Are you dying your agar red? Don’t do that. Red dye has anti fungal properties I think?




I use polyfill with no issues. It's the 1.5hrs PCing that seems to be the issue. 15 minute vent, then pc at 15psi for 2hrs, everytime. Foil over lids to stop polyfill getting wet, then make sure polyfill stays dry throughout, even iso will wick contaminants in.


--------------------





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OfflineDERRAYLD
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Re: How’s my 2nd tub looking? [Re: Screwup] * 1
    #27997993 - 10/14/22 02:36 AM (2 years, 3 months ago)

Quote:

Screwup said:
If you’re using polyfil on your jars while they’re colonizing grain that’s not good enough to keep contaminates out. You need to use Synthetic filter discs.

Are you dying your agar red? Don’t do that. Red dye has anti fungal properties I think?




:justno:

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OfflineScrewup
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Re: How’s my 2nd tub looking? [Re: DERRAYLD]
    #27998015 - 10/14/22 04:08 AM (2 years, 3 months ago)

Which part am I wrong about? I didn’t think polyfill had enough ability to filter contaminates. I also thought it was pretty accepted red dye contains anti fungal or bacterial properties?

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OfflineDERRAYLD
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Re: How’s my 2nd tub looking? [Re: Screwup]
    #27998018 - 10/14/22 04:17 AM (2 years, 3 months ago)

Polyfil is pretty standard filter material for years but you must pack it tightly.

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OfflineThe Tao
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Re: How’s my 2nd tub looking? *DELETED* [Re: Trickymush] * 1
    #27998028 - 10/14/22 05:06 AM (2 years, 3 months ago)

Quote:

Trickymush said:
Post deleted by Trickymush

Reason for deletion: Buried



You asked for advice on a tub and then deleted the opening post with pictures of the tub? Other members learn from seeing successes and failures.
Edit the post to put the pictures back.

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InvisibleFridgedoor
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Re: How’s my 2nd tub looking? *DELETED* [Re: The Tao]
    #27998065 - 10/14/22 05:37 AM (2 years, 3 months ago)

Yeah, that's rather disappointing.


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InvisibleGuerrilla
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Re: How’s my 2nd tub looking? *DELETED* [Re: Fridgedoor]
    #27998077 - 10/14/22 05:47 AM (2 years, 3 months ago)

you must now wear this for the rest of your stay

:shamecube:


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...OR DOES IT?

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Invisiblebakedbeings
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Re: How’s my 2nd tub looking? *DELETED* [Re: Guerrilla]
    #27998087 - 10/14/22 06:02 AM (2 years, 3 months ago)

:monkeyspank:
shame!


i concur polyfil is ok but 90 minutes isnt enough and skip the red dye


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OfflineTrickymush
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Re: How’s my 2nd tub looking? *DELETED* [Re: bakedbeings]
    #27998132 - 10/14/22 06:50 AM (2 years, 3 months ago)

What if you mix yellow and red?

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OfflineScrewup
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Re: How’s my 2nd tub looking? *DELETED* [Re: Trickymush]
    #27998139 - 10/14/22 07:00 AM (2 years, 3 months ago)

?? Just don’t use red. Use any other color IMO.

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Invisiblebakedbeings
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Re: How’s my 2nd tub looking? *DELETED* [Re: Screwup] * 1
    #27998143 - 10/14/22 07:04 AM (2 years, 3 months ago)

i really dont understand using dye at all


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InvisibleGuerrilla
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Re: How’s my 2nd tub looking? *DELETED* [Re: bakedbeings]
    #27998145 - 10/14/22 07:06 AM (2 years, 3 months ago)

Yeah most experienced ppl don't actually use dye.

Ppl talk about contrast and shit but if you know what you're doing it doesn't make a difference.

Colour coding I can kind of understand but proper labelling and organizing does just as well.

I feel like people just want their plates to look cool lols


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Offlinerumfor69
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Re: How’s my 2nd tub looking? *DELETED* [Re: Guerrilla]
    #27998150 - 10/14/22 07:10 AM (2 years, 3 months ago)

I use blue cause it's my favorite color is all lol


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Links For Beginners And Beyond:mspoil:

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Invisiblebakedbeings
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Re: How’s my 2nd tub looking? *DELETED* [Re: rumfor69]
    #27998158 - 10/14/22 07:15 AM (2 years, 3 months ago)

Quote:

rumfor69 said:
I use blue cause it's my favorite color is all lol



the first valid argument ive seen <3


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OfflineScrewup
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Re: How’s my 2nd tub looking? *DELETED* [Re: rumfor69]
    #27998160 - 10/14/22 07:16 AM (2 years, 3 months ago)

Does myc eat the dye in your sigs pics or are you just a phony?

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Re: How’s my 2nd tub looking? *DELETED* [Re: Trickymush]
    #27998186 - 10/14/22 07:38 AM (2 years, 3 months ago)

Quote:

Trickymush said:
What if you mix yellow and red?






DO NOT MIX RED AND YELLOW


I wouldn’t troll here normally, but homie deleted OP after receiving helpful advice. I feel justified.

——
EDIT: my bad. They put it back.
Ima go ahead and not delete those badass snakes, though.

Edited by Egon_Spengler (10/14/22 07:41 AM)

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Offlinerumfor69
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Re: How’s my 2nd tub looking? *DELETED* [Re: Screwup]
    #27998191 - 10/14/22 07:43 AM (2 years, 3 months ago)

Quote:

Screwup said:
Does myc eat the dye in your sigs pics or are you just a phony?




LoL it does eat the blue eventually. But I notice it takes awhile. You can see the center of the left dish has lost its blue.


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InvisibleEgon_Spengler
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Re: How’s my 2nd tub looking? *DELETED* [Re: rumfor69]
    #27998202 - 10/14/22 07:53 AM (2 years, 3 months ago)

A sincere reply this time:

Some red food coloring has been known to display anti-fungal properties. Myc can do weird things on red plates, so lots of just avoid it.

Also: depending on the ingredients of your food coloring, the concentration/amount used, and the aggressiveness of your culture, it doesn’t always take a long time for the myc to start leaching the color out. Timeframes can vary, but it’s totally normal.

These plates were inoculated on 9/28, less than two weeks later they looked like this.






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Invisibledna24
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Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27999324 - 10/14/22 07:10 PM (2 years, 3 months ago)

Quote:

Trickymush said:
I hear you. The only shortcuts I’ve taken was cutting oat pc time from 2 hr to 1 hr 30 min and I believe it’s been causing contams. Plus other shortcut is not doing 3 transfers from MSS plate





this is not the reason for contam, i use oats only do 90mins at 15-17psi and let the jars sit for days to a week and they dont contam in jar. your contam is happening when things are exposed to open air.


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Invisiblebakedbeings
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Re: How’s my 2nd tub looking? [Re: dna24]
    #27999334 - 10/14/22 07:18 PM (2 years, 3 months ago)

Quote:

this is not the reason for contam, i use oats only do 90mins at 15-17psi and let the jars sit for days to a week and they dont contam in jar. your contam is happening when things are exposed to open air.




what are these "things" exactly


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OfflineRusty2096
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Re: How’s my 2nd tub looking? *DELETED* [Re: rumfor69]
    #27999346 - 10/14/22 07:31 PM (2 years, 3 months ago)

Quote:

rumfor69 said:
I use blue cause it's my favorite color is all lol




Than explain why the plates in your signature are turquoise and not blue? :ashamed:


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Offlinerumfor69
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Re: How’s my 2nd tub looking? *DELETED* [Re: Rusty2096]
    #27999395 - 10/14/22 07:52 PM (2 years, 3 months ago)

Quote:

Rusty2096 said:
Quote:

rumfor69 said:
I use blue cause it's my favorite color is all lol




Than explain why the plates in your signature are turquoise and not blue? :ashamed:




Haha it says "teal turquesa" on the package lol


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OfflineTrickymush
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Re: How’s my 2nd tub looking? *DELETED* [Re: rumfor69]
    #27999405 - 10/14/22 07:55 PM (2 years, 3 months ago)

My favorite color in all honesty

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OfflineRusty2096
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Re: How’s my 2nd tub looking? *DELETED* [Re: Trickymush]
    #27999442 - 10/14/22 08:10 PM (2 years, 3 months ago)

Well whoever made that package is quite wise as teal, turquoise and aqua are technically all different colors. But yea - blue crew here as well :super:


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OfflineRusty2096
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Re: How’s my 2nd tub looking? *DELETED* [Re: Screwup]
    #27999444 - 10/14/22 08:11 PM (2 years, 3 months ago)

Quote:

Screwup said:
Does myc eat the dye in your sigs pics or are you just a phony?



:laugh2:


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Offlinerumfor69
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Re: How’s my 2nd tub looking? *DELETED* [Re: Rusty2096]
    #27999452 - 10/14/22 08:18 PM (2 years, 3 months ago)

LOL


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Invisibledna24
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Re: How’s my 2nd tub looking? [Re: bakedbeings]
    #28010285 - 10/22/22 06:27 AM (2 years, 2 months ago)

Quote:

bakedbeings said:
Quote:

this is not the reason for contam, i use oats only do 90mins at 15-17psi and let the jars sit for days to a week and they dont contam in jar. your contam is happening when things are exposed to open air.




what are these "things" exactly




anything sterilized and then opened in an open air environment.


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