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OfflineTrickymush
Mr Man
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Registered: 08/15/22
Posts: 166
Last seen: 1 year, 11 days
Re: How’s my 2nd tub looking? [Re: Screwup]
    #27997221 - 10/13/22 05:42 PM (2 years, 3 months ago)

Bod uses polyfil man. I’ve colonized oyster fine with these polyfil lids I will replace them with new polyfil next time I use though just to be safe. I bought an oyster LC and it had 100% success rate when spawn to straw buckets. Bod wouldn’t tell people to use polyfil lids if it didn’t work

I also heard some grain stuck is fine but I would like to hear someone else’s opinion. The grains stick may be contam like ur saying bc the oyster jars did not have grains stuck.

I just read about the red dye last night but those plates were already made. I forgot I had to order more agar agar so I have to wait for that but I will stop using red just in case but I mean most those plates ate those plates up fine.

I’m not saying you’re wrong on everything but I do have to disagree on the polyfil part. And the red dye thing is kind of up in the air I believe. Just in case I’ll stop using red bc it’s not a big issue to not use it. I’ll probably goto SFD after I use my polyfil up which will honestly be a while and it worked 100% with an isolated oyster LC I bought

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Invisiblecooleko
Augmentum provocatus
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Registered: 08/15/22
Posts: 1,010
Loc: Seattle
Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27997265 - 10/13/22 06:06 PM (2 years, 3 months ago)

Quote:

Trickymush said:
Bod uses polyfil man. I’ve colonized oyster fine with these polyfil lids I will replace them with new polyfil next time I use though just to be safe. I bought an oyster LC and it had 100% success rate when spawn to straw buckets. Bod wouldn’t tell people to use polyfil lids if it didn’t work
Oysters grow so fast they outrun a lot of contamination. Cubes dont.

I also heard some grain stuck is fine but I would like to hear someone else’s opinion. The grains stick may be contam like ur saying bc the oyster jars did not have grains stuck.
As he said, indicator. But I agree that every single jar pictured is contaminated. You really need to pc the full time because grains harbor some resilient contams. Under PCed oats are almost a 100% contam rate for me so I learned to stick with 2-3 hours really fast.

I just read about the red dye last night but those plates were already made. I forgot I had to order more agar agar so I have to wait for that but I will stop using red just in case but I mean most those plates ate those plates up fine.

I’m not saying you’re wrong on everything but I do have to disagree on the polyfil part. And the red dye thing is kind of up in the air I believe. Just in case I’ll stop using red bc it’s not a big issue to not use it. I’ll probably goto SFD after I use my polyfil up which will honestly be a while and it worked 100% with an isolated oyster LC I bought



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OfflineTrickymush
Mr Man
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Registered: 08/15/22
Posts: 166
Last seen: 1 year, 11 days
Re: How’s my 2nd tub looking? [Re: cooleko]
    #27997303 - 10/13/22 06:28 PM (2 years, 3 months ago)

Fair enough I hear you both and I will try some SFD jars in the future. I’ll order some now.

Yeah Reddit has some bullshit info sometimes. That’s where I got the 1 hr 30 min time at. Someone from Reddit said just mist the substrate yet YouTube says mist jus the walls and lid lmao ok which one?

I’m going to start transferring agar plates more and also pcing longer and hopefully those two alone will help me a lot. Only replace polyfil if it looked like it needed replacing

Edited by Trickymush (10/14/22 06:29 AM)

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OfflineNayterade
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Registered: 09/02/22
Posts: 24
Last seen: 2 years, 2 months
Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27997365 - 10/13/22 07:00 PM (2 years, 3 months ago)

Quote:

Trickymush said:
Following bods teks. I did take a shortcut by pcing oat jars for 1 hr 30 min instead of 2 hr so I will go back to 2 hr like I did the first time. Cooking oats now for my g2g I have like 10 master jars to choose from some aren’t active though

I’m going agar to grain spawn to g2g to bulk

Now I will say I haven’t done 3 transfers of agar to spawn so maybe I need to do that. I’ve tried to take shortcut so maybe 2 transfer agar to spawn most times I’ve done first agar plate to spawn





Stop taking shortcuts. Follow Teks to the "T" Chances are if you're taking those shortcuts, you're taking others. Are you using an SAB when you're working with agar and doing tiger drops? Maybe go back to basics and get your technique right, then go to more advanced procedures. I am not saying any of this to be a dick, I'm really just saying, shortcuts get you nowhere, when every single jar you have is contaminated like that. Good luck man, you can do it! Just take your time and do it right. FOLLOW THE TEKS.


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Edited by Nayterade (10/13/22 07:03 PM)

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OfflineTrickymush
Mr Man
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Registered: 08/15/22
Posts: 166
Last seen: 1 year, 11 days
Re: How’s my 2nd tub looking? [Re: Nayterade]
    #27997491 - 10/13/22 08:02 PM (2 years, 3 months ago)

I hear you. The only shortcuts I’ve taken was cutting oat pc time from 2 hr to 1 hr 30 min and I believe it’s been causing contams. Plus other shortcut is not doing 3 transfers from MSS plate

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Offlinecozmyc
gentle modern ape
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Registered: 06/20/21
Posts: 2,154
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Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27997543 - 10/13/22 08:29 PM (2 years, 3 months ago)

Don't forget to vent. I vent at least 15 min now.


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You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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OfflineTrickymush
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Re: How’s my 2nd tub looking? [Re: cozmyc] * 1
    #27997750 - 10/13/22 10:09 PM (2 years, 3 months ago)

Ok I do 10 minutes but fuck it I guess I’ll do 15 since I heard 20 from someone yesterday lol

I appreciate the heads up bc it would’ve been great to hear that if I wasn’t venting. So thanks anyway homie

Edited by Trickymush (10/13/22 10:09 PM)

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Invisiblezmicroinquisitor
donkey
I'm a teapot User Gallery

Registered: 07/27/22
Posts: 141
Loc: Land of Awes
Re: How’s my 2nd tub looking? [Re: Trickymush]
    #27997765 - 10/13/22 10:19 PM (2 years, 3 months ago)

What is this venting you speak of?


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Offlinecozmyc
gentle modern ape
I'm a teapot User Gallery


Registered: 06/20/21
Posts: 2,154
Last seen: 12 days, 10 hours
Re: How’s my 2nd tub looking? [Re: zmicroinquisitor]
    #27997802 - 10/13/22 10:51 PM (2 years, 3 months ago)

When your PC has a good steady stream of steam coming out and the lock locks, set timer for 15-20min, to insure all trapped air is purged. This let's you reach the temp you need.


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You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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OfflineHappinessStan
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Registered: 10/10/12
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Re: How’s my 2nd tub looking? [Re: cozmyc]
    #27997974 - 10/14/22 01:44 AM (2 years, 3 months ago)

Quote:

Screwup said:
If you’re using polyfil on your jars while they’re colonizing grain that’s not good enough to keep contaminates out. You need to use Synthetic filter discs.

Are you dying your agar red? Don’t do that. Red dye has anti fungal properties I think?




I use polyfill with no issues. It's the 1.5hrs PCing that seems to be the issue. 15 minute vent, then pc at 15psi for 2hrs, everytime. Foil over lids to stop polyfill getting wet, then make sure polyfill stays dry throughout, even iso will wick contaminants in.


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OfflineDERRAYLD
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Re: How’s my 2nd tub looking? [Re: Screwup] * 1
    #27997993 - 10/14/22 02:36 AM (2 years, 3 months ago)

Quote:

Screwup said:
If you’re using polyfil on your jars while they’re colonizing grain that’s not good enough to keep contaminates out. You need to use Synthetic filter discs.

Are you dying your agar red? Don’t do that. Red dye has anti fungal properties I think?




:justno:

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OfflineScrewup
Googles your dumb questions
Trans-female

Registered: 01/27/22
Posts: 6,340
Last seen: 10 months, 7 days
Re: How’s my 2nd tub looking? [Re: DERRAYLD]
    #27998015 - 10/14/22 04:08 AM (2 years, 3 months ago)

Which part am I wrong about? I didn’t think polyfill had enough ability to filter contaminates. I also thought it was pretty accepted red dye contains anti fungal or bacterial properties?

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OfflineDERRAYLD
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Re: How’s my 2nd tub looking? [Re: Screwup]
    #27998018 - 10/14/22 04:17 AM (2 years, 3 months ago)

Polyfil is pretty standard filter material for years but you must pack it tightly.

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OfflineThe Tao
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Registered: 09/12/19
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Loc: Canada Flag
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Re: How’s my 2nd tub looking? *DELETED* [Re: Trickymush] * 1
    #27998028 - 10/14/22 05:06 AM (2 years, 3 months ago)

Quote:

Trickymush said:
Post deleted by Trickymush

Reason for deletion: Buried



You asked for advice on a tub and then deleted the opening post with pictures of the tub? Other members learn from seeing successes and failures.
Edit the post to put the pictures back.

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InvisibleFridgedoor
Psssssst!
Male


Registered: 09/13/20
Posts: 1,136
Re: How’s my 2nd tub looking? *DELETED* [Re: The Tao]
    #27998065 - 10/14/22 05:37 AM (2 years, 3 months ago)

Yeah, that's rather disappointing.


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Hokus Pokus Fidibus!

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InvisibleGuerrilla
Original Asshole


Registered: 01/30/21
Posts: 3,179
Loc: United Kingdom Flag
Re: How’s my 2nd tub looking? *DELETED* [Re: Fridgedoor]
    #27998077 - 10/14/22 05:47 AM (2 years, 3 months ago)

you must now wear this for the rest of your stay

:shamecube:


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Being pissed on does not make you a real man.

...OR DOES IT?

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Invisiblebakedbeings
orbiter of truth
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Registered: 09/01/20
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Re: How’s my 2nd tub looking? *DELETED* [Re: Guerrilla]
    #27998087 - 10/14/22 06:02 AM (2 years, 3 months ago)

:monkeyspank:
shame!


i concur polyfil is ok but 90 minutes isnt enough and skip the red dye


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Confused? Well now you can!
HHG - cheapest way to start - how i roll

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OfflineTrickymush
Mr Man
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Registered: 08/15/22
Posts: 166
Last seen: 1 year, 11 days
Re: How’s my 2nd tub looking? *DELETED* [Re: bakedbeings]
    #27998132 - 10/14/22 06:50 AM (2 years, 3 months ago)

What if you mix yellow and red?

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OfflineScrewup
Googles your dumb questions
Trans-female

Registered: 01/27/22
Posts: 6,340
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Re: How’s my 2nd tub looking? *DELETED* [Re: Trickymush]
    #27998139 - 10/14/22 07:00 AM (2 years, 3 months ago)

?? Just don’t use red. Use any other color IMO.

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Invisiblebakedbeings
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Re: How’s my 2nd tub looking? *DELETED* [Re: Screwup] * 1
    #27998143 - 10/14/22 07:04 AM (2 years, 3 months ago)

i really dont understand using dye at all


--------------------
Confused? Well now you can!
HHG - cheapest way to start - how i roll

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