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Offlineuninc4life2010
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I did some P. Mexicana agar to grain (A2G) transfers today. [PICS] * 2
    #27993745 - 10/11/22 01:16 PM (2 years, 3 months ago)



I inoculated 24 oat jars using two plates of clean P. Mexicana Galindoi mycelium.  I made the mistake of leaving the lid off of the plate when doing all of the transfers, so I'm concerned that I may have some issues with contamination.  Also, I'm afraid that 1/12th of an agar plate was too little to inoculate each jar with.  I gave them a good shake to allow as many grains to contact the wedge, but that may not be enough to ensure fast colonization.  Whatever happens, I'll do a mini-grow log and update you all on how they do.

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OfflineJaksavage
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Re: I did some P. Mexicana agar to grain (A2G) transfers today. [PICS] [Re: uninc4life2010]
    #27993924 - 10/11/22 03:42 PM (2 years, 3 months ago)

That is what I call commitment!

Be patient it might not be quick. lol


:goodluck:


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Offlineuninc4life2010
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Re: I did some P. Mexicana agar to grain (A2G) transfers today. [PICS] [Re: Jaksavage]
    #27994121 - 10/11/22 05:54 PM (2 years, 3 months ago)

Quote:

Jaksavage said:
That is what I call commitment!

Be patient it might not be quick. lol


:goodluck:




We all will collectively see.  I think that a lot of the jars will be okay.

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OfflineRusty2096
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Re: I did some P. Mexicana agar to grain (A2G) transfers today. [PICS] [Re: uninc4life2010]
    #27994211 - 10/11/22 06:35 PM (2 years, 3 months ago)

Dude did you oil your agar or something? HOW is it now sticking to the glass :shrug:

You are a wizard for sure

:wizard:

Edit: Or did you just drop and not shook the jars after?


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Edited by Rusty2096 (10/11/22 06:36 PM)

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Offlineuninc4life2010
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Re: I did some P. Mexicana agar to grain (A2G) transfers today. [PICS] [Re: Rusty2096]
    #27994283 - 10/11/22 07:32 PM (2 years, 3 months ago)

Quote:

Rusty2096 said:
Dude did you oil your agar or something? HOW is it now sticking to the glass :shrug:

You are a wizard for sure

:wizard:

Edit: Or did you just drop and not shook the jars after?




The pic of the jars inside thee SAB was before they were shaken.  I didn't have too much trouble with the agar sticking.  Some got stuck to the top of the jar lid, but that was really it.

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InvisibleWinston Smith
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Re: I did some P. Mexicana agar to grain (A2G) transfers today. [PICS] [Re: uninc4life2010]
    #27994291 - 10/11/22 07:36 PM (2 years, 3 months ago)

Badass, dude! I got some mex on agar about ready for grain too, I think. If you could update on colonization times that would be killer. No stress though, just curious what I should expect ha!

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OfflineAwestruck
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Re: I did some P. Mexicana agar to grain (A2G) transfers today. [PICS] [Re: uninc4life2010]
    #27994304 - 10/11/22 07:40 PM (2 years, 3 months ago)

Nice, man! That's a good amount of jars. You shooting for fruits or sclerotia? Both?


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Offlineuninc4life2010
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Re: I did some P. Mexicana agar to grain (A2G) transfers today. [PICS] [Re: Awestruck]
    #27994460 - 10/11/22 09:18 PM (2 years, 3 months ago)

Quote:

Awestruck said:
Nice, man! That's a good amount of jars. You shooting for fruits or sclerotia? Both?




Sclerotia.  Way less of a pain to deal with, and it's a lot more discreet.  It's also much less expensive since I don't need bulk substrate or tubs/fruiting chambers.

Edited by uninc4life2010 (10/11/22 09:19 PM)

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OfflineAwestruck
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Re: I did some P. Mexicana agar to grain (A2G) transfers today. [PICS] [Re: uninc4life2010]
    #27994812 - 10/12/22 07:47 AM (2 years, 3 months ago)

For sure. Easy to just set and forget for months.


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Repaying my karmic debt to the Shroomery.
Looking for a Pan cambo print.

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OfflinePrestoPesto
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Re: I did some P. Mexicana agar to grain (A2G) transfers today. [PICS] [Re: Awestruck]
    #27995006 - 10/12/22 10:40 AM (2 years, 3 months ago)

Definitely following this thread since I also have some Mex agar going and I’m curious about timeline too


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“In theory, practice and theory are the same. In practice, they rarely are.”

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Offlineuninc4life2010
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Re: I did some P. Mexicana agar to grain (A2G) transfers today. [PICS] [Re: PrestoPesto]
    #27995143 - 10/12/22 12:08 PM (2 years, 3 months ago)

Quote:

PrestoPesto said:
Definitely following this thread since I also have some Mex agar going and I’m curious about timeline too




Cool!  Last night, I spent some more time thinking about this, and I inoculated 12 more jars using two 100mmm agar plates, so 6 jars per plate.  I wanted to compare how fast they colonize to see if it's worth using larger agar slices.  It may be, it may not be.

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Offlineuninc4life2010
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Re: I did some P. Mexicana agar to grain (A2G) transfers today. [PICS] [Re: PrestoPesto]
    #27995165 - 10/12/22 12:28 PM (2 years, 3 months ago)

Quote:

PrestoPesto said:
Definitely following this thread since I also have some Mex agar going and I’m curious about timeline too




Here is the new post I made for the experiment.

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Offlineuninc4life2010
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Re: I did some P. Mexicana agar to grain (A2G) transfers today. [PICS] [Re: uninc4life2010]
    #28002336 - 10/16/22 08:00 PM (2 years, 2 months ago)

Just giving an update at day 5.  There looks to be an expected amount of colonization.  Two of the jars are undoubtedly contaminated, but all others look fine. 


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