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OfflineBajazly
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Dumb Idea??? Update and Another Update * 1
    #27981819 - 10/04/22 07:45 PM (2 years, 3 months ago)

I put this over on Agar Envy but I think more may see it here.

Alright, next dumb question. I just noticed I've got an ugly green patch in one of my PF jars, 2 down 10 to go but whatever. I also have a bunch of poured agar cups sitting in the fridge waiting to grow something.

Would it be dumb to take this jar out side, open it up and cut a piece of myc off the opposite side of the green blob and throw in a few dishes to see what happens?

Maybe pour some 140º water agar over the top of it?

How would you do it open air? Cut it off and throw it in some purified water and put that in the SAB to slice it up to distribute it between several cups once everything is ready?

I have way too much time and want to make the most of the resources I have to get other projects going and don't mind trying out of the box things to make it happen.

Is this a sure fail or worth trying?

Edited by Bajazly (10/22/22 01:57 PM)

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Invisibledirtbagauds
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Re: Dumb Idea??? [Re: Bajazly] * 1
    #27981840 - 10/04/22 07:56 PM (2 years, 3 months ago)

so you plan to dissect the healthy mycelium from the plate and transfer it to a new plate to continue colonization?

or adding it to PF jars?

Regardless, my understanding is that as long as you isolate healthy mycelium you should be able to complete a transfer to sub jars or new agar plates without contam!

Goodluck!


--------------------
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OfflineBajazly
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Re: Dumb Idea??? [Re: dirtbagauds]
    #27981894 - 10/04/22 08:17 PM (2 years, 3 months ago)

Quote:

dirtbagauds said:
so you plan to dissect the healthy mycelium from the plate and transfer it to a new plate to continue colonization?

or adding it to PF jars?

Regardless, my understanding is that as long as you isolate healthy mycelium you should be able to complete a transfer to sub jars or new agar plates without contam!

Goodluck!




Yeah, take a piece of good myc from the PF jar far away from the contamination and put it to agar to see what happens.

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InvisibleNichrome
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Re: Dumb Idea??? [Re: Bajazly] * 1
    #27982026 - 10/04/22 09:47 PM (2 years, 3 months ago)

You could very well do that and get a good culture. You'll likely have to transfer a couple of times before it is clean enough to use.


--------------------
discussions are a healthy alternative to arguments

There is only one electron, and it's you.

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OfflineBajazly
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Re: Dumb Idea??? [Re: Nichrome]
    #27982096 - 10/04/22 10:24 PM (2 years, 3 months ago)

Went ahead and did it tonight. The green was moving fast so I hope I got ahead of it in time. Put a little piece to 6 plates so we will see what happens.

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InvisibleNichrome
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Re: Dumb Idea??? [Re: Bajazly] * 2
    #27982107 - 10/04/22 10:35 PM (2 years, 3 months ago)

Just pay attention and keep ready to make small transfers in just a few days. If you stay on it you can clean that up. With 6 plates to start with your chances are pretty good.


--------------------
discussions are a healthy alternative to arguments

There is only one electron, and it's you.

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OfflineBajazly
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Re: Dumb Idea??? [Re: Nichrome]
    #27982679 - 10/05/22 11:29 AM (2 years, 3 months ago)

Thank you, I will keep an eye on them and see what happens and report back.

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OfflineBajazly
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Re: Dumb Idea??? [Re: Bajazly]
    #27984646 - 10/06/22 01:16 PM (2 years, 3 months ago)

After a few days here is where I'm at. Looking viable or more like junk? I know I probably used too big of pieces but first time and all. The first one looks better to me but I've only seen pictures of this stuff so no idea.




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Invisiblebakedbeings
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Re: Dumb Idea??? [Re: Bajazly]
    #27984658 - 10/06/22 01:22 PM (2 years, 3 months ago)

take transfers from that top pic, anywhere between 12 oclock and 6 on the right side


--------------------
Confused? Well now you can!
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OfflineBajazly
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Re: Dumb Idea??? [Re: bakedbeings]
    #27984672 - 10/06/22 01:28 PM (2 years, 3 months ago)

Quote:

Bakedbeings said:
take transfers from that top pic, anywhere between 12 oclock and 6 on the right side




Give it another day or so or prep everything and do it now?

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Invisiblebakedbeings
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Re: Dumb Idea??? [Re: Bajazly]
    #27984681 - 10/06/22 01:33 PM (2 years, 3 months ago)

whichever is convenient


--------------------
Confused? Well now you can!
HHG - cheapest way to start - how i roll

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OfflineBajazly
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Re: Dumb Idea??? [Re: bakedbeings]
    #27984690 - 10/06/22 01:44 PM (2 years, 3 months ago)

Muchas gracias. My scalpel just arrived and I need to make a little stainless straw for cutting rounds so I'll do that this afternoon.

QQ, the plate I'm transferring to is the T1 or T2 plate? Haven't figured out what the T numbers mean exactly other than a higher number is a subsequent plate to the previous.

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Invisiblebakedbeings
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Re: Dumb Idea??? [Re: Bajazly]
    #27984697 - 10/06/22 01:47 PM (2 years, 3 months ago)

you dont "need" a straw


--------------------
Confused? Well now you can!
HHG - cheapest way to start - how i roll

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OfflineBajazly
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Re: Dumb Idea??? [Re: bakedbeings]
    #27984701 - 10/06/22 01:49 PM (2 years, 3 months ago)

Ok, scalpel it is.
Thanks

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InvisibleTexastransplant
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Re: Dumb Idea??? [Re: Bajazly]
    #27984773 - 10/06/22 02:27 PM (2 years, 3 months ago)

Quote:

Bajazly said:
Muchas gracias. My scalpel just arrived and I need to make a little stainless straw for cutting rounds so I'll do that this afternoon.

QQ, the plate I'm transferring to is the T1 or T2 plate? Haven't figured out what the T numbers mean exactly other than a higher number is a subsequent plate to the previous.




T# = the times you made a transfer (AFAIK), which helps you predict senescence of the genetic line you are working on. But I could be completely wrong as I am new at the game as well, just reading.

I have leather punch tools I am going to repropose instead of straws, but again, I had them already.

Edited by Texastransplant (10/06/22 02:29 PM)

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Offlinesmalltalk_canceled
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Re: Dumb Idea??? [Re: Bajazly]
    #27984859 - 10/06/22 03:28 PM (2 years, 3 months ago)

Quote:

Bajazly said:
I put this over on Agar Envy but I think more may see it here.

Alright, next dumb question. I just noticed I've got an ugly green patch in one of my PF jars, 2 down 10 to go but whatever. I also have a bunch of poured agar cups sitting in the fridge waiting to grow something.

Would it be dumb to take this jar out side, open it up and cut a piece of myc off the opposite side of the green blob and throw in a few dishes to see what happens?

Maybe pour some 140º water agar over the top of it?

How would you do it open air? Cut it off and throw it in some purified water and put that in the SAB to slice it up to distribute it between several cups once everything is ready?

I have way too much time and want to make the most of the resources I have to get other projects going and don't mind trying out of the box things to make it happen.

Is this a sure fail or worth trying?




havent read anything but this post, and just want to tell you, that isolating away from sporulating molds, like the green trich, is a very painful and treacherous action to take. what you cant see is still there.

as a beginner, try to avoid wasting time on isolating away from green molds - it is 100% not worth the effort. even experienced mycologists, farmers, growers, whatever, know that this exercise is a hard one.

we only do it when we are desperate to save genetics/culture

as a beginner things are hard enough if you're not spending your time trying to isolate away from green molds

instead - look for a better germination/starting point


--------------------
Willpower is the one true virtue


Edited by smalltalk_canceled (10/06/22 03:29 PM)

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OfflineBajazly
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Re: Dumb Idea??? [Re: smalltalk_canceled]
    #27984887 - 10/06/22 03:46 PM (2 years, 3 months ago)

Quote:

smalltalk_canceled said:
Quote:

Bajazly said:
I put this over on Agar Envy but I think more may see it here.

Alright, next dumb question. I just noticed I've got an ugly green patch in one of my PF jars, 2 down 10 to go but whatever. I also have a bunch of poured agar cups sitting in the fridge waiting to grow something.

Would it be dumb to take this jar out side, open it up and cut a piece of myc off the opposite side of the green blob and throw in a few dishes to see what happens?

Maybe pour some 140º water agar over the top of it?

How would you do it open air? Cut it off and throw it in some purified water and put that in the SAB to slice it up to distribute it between several cups once everything is ready?

I have way too much time and want to make the most of the resources I have to get other projects going and don't mind trying out of the box things to make it happen.

Is this a sure fail or worth trying?




havent read anything but this post, and just want to tell you, that isolating away from sporulating molds, like the green trich, is a very painful and treacherous action to take. what you cant see is still there.

as a beginner, try to avoid wasting time on isolating away from green molds - it is 100% not worth the effort. even experienced mycologists, farmers, growers, whatever, know that this exercise is a hard one.

we only do it when we are desperate to save genetics/culture

as a beginner things are hard enough if you're not spending your time trying to isolate away from green molds

instead - look for a better germination/starting point




Thank you smalltalk, duly noted. I have tons of time and at the moment I am out of anything to start with and anything new is a few weeks away. Figured no loss in trying and its good practice working in the SAB, which I hate but a necessary evil I know. First time with agar and it seems maybe I might get lucky here, a few days will tell.

Again, thanks for contributing to my future knowledge base.

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Offlinesmalltalk_canceled
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Re: Dumb Idea??? [Re: Bajazly]
    #27984898 - 10/06/22 03:54 PM (2 years, 3 months ago)

Ill happily send you a print if you use up your current starting point,
and many many other people here on the shroomery are of alike mind.

Ask and it shall be given, but dont waste your time trying to clean up green molds if you are new. go for a better start

however, doing agar work with what you have, waiting for replacement,
shows enthusiasm. i applaud this. but dont spend it all on moldy plates, save some energy for a good start.

be blessed


--------------------
Willpower is the one true virtue


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OfflineBajazly
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Registered: 09/02/22
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Re: Dumb Idea??? [Re: smalltalk_canceled]
    #27984908 - 10/06/22 04:01 PM (2 years, 3 months ago)

Quote:

smalltalk_canceled said:
Ill happily send you a print if you use up your current starting point,
and many many other people here on the shroomery are of alike mind.

Ask and it shall be given, but dont waste your time trying to clean up green molds if you are new. go for a better start

however, doing agar work with what you have, waiting for replacement,
shows enthusiasm. i applaud this. but dont spend it all on moldy plates, save some energy for a good start.

be blessed




You are too kind smalltalk, thank you, I will take you up on that offer. I'll pm you my address and make an inoculation loop and read up on spore prints while I'm waiting.

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OfflineBajazly
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Re: Dumb Idea??? [Re: Bajazly]
    #27992451 - 10/10/22 05:44 PM (2 years, 3 months ago)

Update on the Dumb Idea.

The first two pics are the plates I made transfers from, let them sit around for a few days just to see how they developed, obviously got a little comtam going in there.





The next 4 are of the transfer plates, 2 from each plate did pretty well it looks like? It's been 3 days since the transfer. Do these actually look good and if so should I do another transfer or let these go a while and if they still look good put them onto something?

And for clarity in my head, are the plates I transferred to the T1 or T2 plates? ie, is the first plate T0?








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