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OfflineBobgas
Master of Mistakes

Registered: 09/01/22
Posts: 585
Last seen: 4 months, 5 days
Ready for g2g?
    #27979421 - 10/03/22 11:39 AM (2 years, 3 months ago)

Seems I have a good jar, so I want to move it to 3 others.  Am I missing contam because I don't see it?  I shook 3 days ago from 25% and this is where it's at.  The myc took off at light speed.

I do see a bit of condensation at the top of the jar, but no yellow/orange throughout the rest.  I also dont see any wet/pressed grains.

When is the best time to g2g?  Is this ready?  Do I need to wait for more colonization? 

Thanks!

Here are picts of the 4 sides of the jar:


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OfflineDr. Jones
Pilgrim in an unholy land

Registered: 01/07/22
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Re: Ready for g2g? [Re: Bobgas]
    #27979435 - 10/03/22 11:51 AM (2 years, 3 months ago)

If the mycelium took off at light speed following a shake, contamination likely isn't an issue. In my experience and based on previous scouring of the forums you'll see stunted colonization with contamination.

Those don't appear to be fully colonized so waiting isn't going to hurt. If your overly anxious to get on with it I think g2g would be fine at this point providing you follow the golden rule, good sterile technique.

Edited by Dr. Jones (10/03/22 11:52 AM)

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InvisibleKnateKing89
Stranger

Registered: 06/29/22
Posts: 110
Re: Ready for g2g? [Re: Bobgas]
    #27979437 - 10/03/22 11:52 AM (2 years, 3 months ago)

It is recommended to wait for full colonization before G2G.
That being said I have G2G before full colonization with no problems.

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OfflineScrewup
Googles your dumb questions
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Re: Ready for g2g? [Re: KnateKing89] * 1
    #27979441 - 10/03/22 11:54 AM (2 years, 3 months ago)

That doesn’t look the greatest to me but maybe I’m wrong?

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Offlinenormalperson
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Re: Ready for g2g? [Re: Bobgas]
    #27979447 - 10/03/22 11:59 AM (2 years, 3 months ago)

G2G with Oats is risky but if that's what you want to do definitely give the jar another shake or two. When the myc covers all the grains equally post another picture, your jar is not at 100% colonization yet.

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InvisibleKnateKing89
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Registered: 06/29/22
Posts: 110
Re: Ready for g2g? [Re: normalperson]
    #27979505 - 10/03/22 12:49 PM (2 years, 3 months ago)

Quote:

Screwup said:
That doesn’t look the greatest to me but maybe I’m wrong?




Healthy cube mycelium on Grain is typically thinner and whispier. When it is bright white thick and ropey IME it has almost always been bacterial. I have had a few clones in the past that were more rizomorphic but most of the time were quite whispy. Looks about right to me for 3 days recovery



Quote:

normalperson said:
G2G with Oats is risky but if that's what you want to do definitely give the jar another shake or two. When the myc covers all the grains equally post another picture, your jar is not at 100% colonization yet.




Why exactly is it risky to G2G oats? This is news to me.

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OfflineBobgas
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Registered: 09/01/22
Posts: 585
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Re: Ready for g2g? [Re: normalperson]
    #27979832 - 10/03/22 05:22 PM (2 years, 3 months ago)

Thanks - that is what I wanted to know.  I will allow it to colonize further and will do one more shake tonight.

Yeah, I am moving to agar - I have 50+ poured plates that I will be inoculating one day this week with mss.  Between the time it will take to get clean agar to from a2g, I hope to have 3 tubs of fruiting in progress.


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Offlineuninc4life2010
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Re: Ready for g2g? [Re: Bobgas] * 2
    #27979932 - 10/03/22 06:18 PM (2 years, 3 months ago)

Yeah, those jars definitely don't look contaminated, but they also don't look fully colonized.  I would wait until they look more like what you see below.  This game is all about patience.

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Offlinethumbsy
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Re: Ready for g2g? [Re: uninc4life2010]
    #27980060 - 10/03/22 07:24 PM (2 years, 3 months ago)

I do g2g using oats. Its same as any other whole grain imo, just be meticulous about hydration and sterilization... same as any other grain process.

I'll agree that those jars don't seem to be at the "fully colonized" looking stage that I'd be wanting to see them at to do a g2g. I'm kinda on the fence looking at them, possibly looking bacterial. Few more days of observation is in order.


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Edited by thumbsy (10/05/22 07:08 PM)

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OfflineBobgas
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Re: Ready for g2g? [Re: uninc4life2010]
    #27980065 - 10/03/22 07:27 PM (2 years, 3 months ago)

OMG - those look absolutely fantastic.  Congrats!  Thanks for showing me what it needs to look like.  That's helpful.

I am also working on understanding how to dial a tub so that I am ready for quart jars.  I shredded pf cakes into a 7qt container with coir.  Good grief, this hobby is a massive learning curve.... but the right tools sure help and the people's willingness to share is invaluable.


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OfflineBobgas
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Re: Ready for g2g? [Re: thumbsy]
    #27980076 - 10/03/22 07:33 PM (2 years, 3 months ago)

Thanks.  Yup - bacteria is certainly possible.  This is mss ...  The jar has been pretty clear up to now - there are no wet grains stuck to the glass, no myc piss and the grains move as individuals.

I ran a total of 10 grain jars. I only used .5-1cc per jar to stretch what I had and to have enough for agar.

8 never colonized and the oats look very good to me - they roll as individual grains, appear to be nice and dry and the jars are perfectly clear... no condensation or slime at all.  So, I am hopeful, but know it could as easily go tits up.  If there is contam, I will know before g2g and perhaps can still fruit this one jar in it's own 7qt tub.


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InvisibleKnateKing89
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Re: Ready for g2g? [Re: Bobgas]
    #27982751 - 10/05/22 12:35 PM (2 years, 3 months ago)

Quote:

Bobgas said:
I am also working on understanding how to dial a tub so that I am ready for quart jars.  I shredded pf cakes into a 7qt container with coir.  Good grief, this hobby is a massive learning curve.... but the right tools sure help and the people's willingness to share is invaluable.




Dialing in is very simple. The idea is to give as much fresh air as possible without drying it out. This can be done very easily in many ways from fanning by hand to more passive ways such as drilling holes or cracking the lid a bit. You want little beads of water on the surface of the substrate at all times. once dried out it can be difficult to get it to bead like before and excessive misting can cause matting which makes it harder to gauge surface moisture. Watch it closely for the first week after full colonization If the beads start getting smaller give it less air as that means it is evaporating too fast. Less passive FAE can be achieved in many ways as well such as taping up holes adding layers of micropore tape tightening polyfill putting the lid on tight ect. What type of fruiting chamber do you plan on using? Also what is the ambient RH in the grow room? With this information we can help you get pretty close to dialed in right off the bat and you shouldn't have to tweak much to get it dialed in. Lets get an update on how those jars are coming along today! :smile:

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OfflineBobgas
Master of Mistakes

Registered: 09/01/22
Posts: 585
Last seen: 4 months, 5 days
Re: Ready for g2g? [Re: KnateKing89]
    #27983762 - 10/05/22 10:12 PM (2 years, 3 months ago)

Thanks!  I'll post pictures tomorrow morning.  The jar I shook has recovered very well and I will have to make the choice of spawning it or g2g into 4 other grain jars I have prepped.  I shot mss to 20 plates this Tues, so I have time to play until my agar/a2g process takes over.

If I spawn the one, I plan to use a 7qt tub and mix 1:3 with straight coir (which I already have prepped.)  If I do g2g, I plan to use a 67qt tub for the 4 that will colonize and I will have to make up more coir to get 1:3.

Right now, I have 2 7qt tubs I shredded brf cakes into and mixed with coir and then put a 1/2" layer of straight coir on top to practice.  It is not going as well as I'd hoped.

I need to learn (among many other thnigs):

When spawned and mixed, do I then put a 1/2" layer of fc coir on top?
Do I keep the lid tight on the tub with no fae until full colonization of the spawn/coir and then intro FAE for fruiting?  Or do I start with fae straight away?  Right now, my tubs are all unmodified and I was hoping to keep it that way.

I have read too many threads. :frown:


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πŸ…ƒπŸ„΄πŸ„°πŸ„Ό  πŸ„²πŸ„»πŸ„ΈπŸ„½ πŸ„ΆπŸ…†πŸ…πŸ„°πŸ„Ώ

Edited by Bobgas (10/05/22 10:17 PM)

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OfflineBobgas
Master of Mistakes

Registered: 09/01/22
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Re: Ready for g2g? [Re: Bobgas]
    #27984209 - 10/06/22 08:22 AM (2 years, 3 months ago)

Here is the state of the most colonized jar.  I want to g2g, but I also want to get some fruit going! It looks great to me, but maybe I am missing something. I will let it colonize further before making a choice.



Here is a cake that would be on it's 3rd flush.  It previously fruited in a tub of pearlite, but I wanted to try something else.... so I put it in coir after a dunk and in a ziplock bag.  It's going nuts colonizing the coir!  But, I don't see any pins.  The bag has tons of holes poked in it and I have the top open 1 inch, but no fruiting.  I am not quite sure what to change at this point.



And, here are the two 7qt tubs I have going.  These were brf cakes that I dunked and shredded and mixed 2:1 with fc coir.  The myc seems to be ccolonizing - although the process seems to have slowed just a bit.  Initially I had the lids off-center by a 1/4", but the surface droplets disappeared pretty quickly. After 3 days, I closed the lids to try something different and now the surface seems correct, but the growth is not as vigorous. The tubs are unmodified.  Again, this is to practice before the grain jars move to tubs.



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InvisibleKnateKing89
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Registered: 06/29/22
Posts: 110
Re: Ready for g2g? [Re: Bobgas]
    #27984521 - 10/06/22 11:57 AM (2 years, 3 months ago)

Quote:

Bobgas said:

When spawned and mixed, do I then put a 1/2" layer of fc coir on top? :frown:




If it is a real casing wait till full colonization if its a pseudo casing (cvg,cv or c) then put it on when you spawn.


Quote:

Bobgas said:

Do I keep the lid tight on the tub with no fae until full colonization of the spawn/coir and then intro FAE for fruiting?  Or do I start with fae straight away?  Right now, my tubs are all unmodified and I was hoping to keep it that way. :frown:




If you are using an unmodified tub then keep the lid on till full colonization.

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InvisibleKnateKing89
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Registered: 06/29/22
Posts: 110
Re: Ready for g2g? [Re: Bobgas]
    #27984534 - 10/06/22 12:08 PM (2 years, 3 months ago)

Quote:

Bobgas said:
Here is the state of the most colonized jar.  I want to g2g, but I also want to get some fruit going! It looks great to me, but maybe I am missing something. I will let it colonize further before making a choice.






You might be able to get away with G2G that jar. Looks a tad Bacterial to me but may not be. If it was mine I would spawn it and wait and make transfers from the plates to make sure it is clean before making a master jar.

Quote:

Bobgas said:
Here is a cake that would be on it's 3rd flush.  It previously fruited in a tub of pearlite, but I wanted to try something else.... so I put it in coir after a dunk and in a ziplock bag.  It's going nuts colonizing the coir!  But, I don't see any pins.  The bag has tons of holes poked in it and I have the top open 1 inch, but no fruiting.  I am not quite sure what to change at this point.

 




That cake has used alot of its nutes already. In the future don't spawn used cakes to new coir. Just find a nice shady spot outside and start dumping all your spent subs and cakes there.

Quote:

Bobgas said:
And, here are the two 7qt tubs I have going.  These were brf cakes that I dunked and shredded and mixed 2:1 with fc coir.  The myc seems to be ccolonizing - although the process seems to have slowed just a bit.  Initially I had the lids off-center by a 1/4", but the surface droplets disappeared pretty quickly. After 3 days, I closed the lids to try something different and now the surface seems correct, but the growth is not as vigorous. The tubs are unmodified.  Again, this is to practice before the grain jars move to tubs.







Next time leave the lid on while it is colonizing. To me they look a little dry and might be why it has stalled.

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InvisibleKnateKing89
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Registered: 06/29/22
Posts: 110
Re: Ready for g2g? [Re: Bobgas]
    #27984554 - 10/06/22 12:20 PM (2 years, 3 months ago)

Quote:

Bobgas said:

I have read too many threads. :frown:




Find one tek and follow it to a T. Many of the TCs have great grow logs to follow. Frank pasty cronicr and bod just to name a few have really great relevant info from 2013-Current. Or you could just UTSF and make sure you click the TC box, Filter posts from 4 years and current as well as clicking the box that says show one post per thread. You will easily find a current up to date grow log to follow.

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OfflineBobgas
Master of Mistakes

Registered: 09/01/22
Posts: 585
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Re: Ready for g2g? [Re: KnateKing89]
    #27985641 - 10/06/22 10:07 PM (2 years, 3 months ago)

Greatly appreciate the knowledge.


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OfflineBobgas
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Re: Ready for g2g? [Re: Bobgas]
    #27986752 - 10/07/22 05:33 PM (2 years, 3 months ago)

Ready to spawn or should I wait a bit more. I've decided to take this to a tub (as long as someone doesn't come along and tell me it's got to be tossed!!)

I plan to do an unmodified 7qt tub 1:3 straight coir.



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OfflineRusty2096
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Re: Ready for g2g? [Re: Bobgas]
    #27986763 - 10/07/22 05:37 PM (2 years, 3 months ago)

Very likely bacterial.

No need to toss you prob will get fruits in a tub. Maybe do 1:2 spawn:sub ration to be on the safe side. 1:3 is also fine.


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Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

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Offlinefiddle_head
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Re: Ready for g2g? [Re: Bobgas]
    #27986801 - 10/07/22 05:54 PM (2 years, 3 months ago)

I have some oat jars that look exactly like this. I spawned slightly bacterial jars into 6 qt CVG, and one in a shoebox at 1:2.

The other ones I have are looking kind of wispy with some uncolonized portions of grains against the sides of the jars. Around 60% colonized.

In the end I think it was because I didn't vent my PC properly or a lapse in sterile technique.

Point is, my jars look almost identical to yours and they are colonizing the tub and shoebox quite nicely after 24 hours. You may have some luck. Oats can be tricky.


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OfflineBobgas
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Re: Ready for g2g? [Re: fiddle_head]
    #27987170 - 10/07/22 09:09 PM (2 years, 3 months ago)

Thanks.  I will spawn tomorrow.

The crap in the top of the jar is myc that separated from the grains in the multiple shakes.  This jar was incredibly aggressive in recovering from a shake.  I am sure there is some bacteria, but I am not sure what I am looking at to see it.

I have 9 other jars from this batch of oats where all but one other are barely getting started and they all are totally clear.

While I was considering g2g, because the other jars did not start, lo and behold all but one started growing myc.

Like an idiot, I did not label these jars so I am making the bet that the colonized jar is GT while the others are APE, PE and B+.  It will be interesting to see how the others get on.

I hope to get some fruit!


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OfflineBobgas
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Re: Ready for g2g? [Re: Bobgas]
    #27988194 - 10/08/22 12:59 PM (2 years, 3 months ago)

I spawned the jar today.  Yup, I get it's likely bacterial to an extent.

It is mixed with ~1:3 straight coir, lightly tamped and leveled and I then added a 1/4" layer of straight coir.

The tub has 3 holes near the top on the short/handle sides and 2 each on the long sides about 1.5" up from the substrate. The lid is closed.

Can someone please let me know what I should do differently at this stage- ie. Should I cover the holes with mp tape?

Thanks!



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InvisibleKnateKing89
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Re: Ready for g2g? [Re: Rusty2096]
    #28000308 - 10/15/22 12:14 PM (2 years, 3 months ago)

Quote:

Rusty2096 said:
Very likely bacterial.

No need to toss you prob will get fruits in a tub. Maybe do 1:2 spawn:sub ration to be on the safe side. 1:3 is also fine.





Hopefully you spawned these OP. They do look a little bacterial but not so bad that they won't produce mush. Got an updated pic of the tub?

Edit: Never mind saw the post of you spawning them after posting this lol. Would still like to see the updated pics should be close to 100% by now.

Edited by KnateKing89 (10/15/22 12:15 PM)

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OfflineBobgas
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Re: Ready for g2g? [Re: KnateKing89]
    #28000867 - 10/15/22 07:28 PM (2 years, 3 months ago)

Thanks for checking in.  I'll post pictures tomorrow.  It's not quite 100%.  The substrate looks essentially colonized, but the surface still has a couple of spots to fill in.  Tomorrow is 7 days.

So far the surface looks like myc and coir.


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Edited by Bobgas (10/15/22 07:29 PM)

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Offlinecozmyc
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Re: Ready for g2g? [Re: Bobgas]
    #28000899 - 10/15/22 07:49 PM (2 years, 3 months ago)

:cheers:
:popcorn:


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OfflineBobgas
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Re: Ready for g2g? [Re: cozmyc]
    #28001512 - 10/16/22 08:31 AM (2 years, 2 months ago)

Here is the current state of the tub. I have no idea what im doing regarding providing proper surface conditions, but I'm assuming it's more important at this point to allow it to fully colonize.

Please let me know if there is something nasty I am just missing.

Oddly the picture quality is terrible. There is no gray in the tub... that must be shadows.



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Edited by Bobgas (10/16/22 08:38 AM)

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OfflineScrewup
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Re: Ready for g2g? [Re: Bobgas]
    #28001513 - 10/16/22 08:31 AM (2 years, 2 months ago)

Just keep surface conditions up and leave it alone. Time will tell.

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OfflineBobgas
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Re: Ready for g2g? [Re: Screwup]
    #28007595 - 10/20/22 09:09 AM (2 years, 2 months ago)

Here is where I'm at.  This is at 11 days.  I know I should be seeing beads, but I don't see them. I am not sure what I need to actually do.

I taped the holes (covered 1/2 of each hole and completely covered one hole on each of the sides) and have left the tub alone.  That seems to have helped a bit, but still no beads?



There are other jars a day or two away from spawning, some quite a few days away and my pftek is chugging along.  I have GT that appears it will be ready to spawn first and PES that is coming in a close second place.  The others are steadily moving along, but slowly.



I also have agar plates in a couple of states (T1 that need a T2, and a couple of plates that I just started from spores that need a couple of days before a T1.). I have to make more plates!


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Edited by Bobgas (10/20/22 09:56 AM)

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Offlinecozmyc
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Re: Ready for g2g? [Re: Bobgas]
    #28007701 - 10/20/22 10:39 AM (2 years, 2 months ago)

I think I see some surface moisture in the first pic :thumbup: if those dissappear,  mist lightly other than that, any day now


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----------------------
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Offlineschpat
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Re: Ready for g2g? [Re: cozmyc]
    #28008608 - 10/21/22 12:34 AM (2 years, 2 months ago)

yeah, if you don't see glistening moisture then apply gentle mist.


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OfflineBobgas
Master of Mistakes

Registered: 09/01/22
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Re: Ready for g2g? [Re: schpat]
    #28008850 - 10/21/22 06:42 AM (2 years, 2 months ago)

Thanks. Will do.  I am also taking a second transfer today of my candidate plates.  I’ excited I am moving toward knowing my spawn is clean.


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OfflineBobgas
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Re: Ready for g2g? [Re: Bobgas]
    #28015501 - 10/25/22 06:35 AM (2 years, 2 months ago)

14 days and not a single pin. As far as I can tell, the surface conditions are what they are supposed to be. I'm at a loss.

The myc is all white. The grey color is just a shadow. I'm trying not to touch the box at all.



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Edited by Bobgas (10/25/22 06:37 AM)

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OfflineBobgas
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Re: Ready for g2g? [Re: Bobgas]
    #28015600 - 10/25/22 08:37 AM (2 years, 2 months ago)

I am also making progress with my agar work.  I am trying to get to clean B+, GT, PE and APE.  I am on T2.  This was a T1 plate that I took a couple of samples from.

A couple of questions:
How many grain jars (qt oat) can I make with a single plate? Do I use a plate per jar or some fraction of that?

How many transfers is it normal to take from a single plate?  I took 2 to create new plates.  Is that normal?

Once I am ready to go to grain, is it better to make another plate that would act as a master and create a slant of, or is it better to clone the fruit back to agar once I have seen the performance of the fruiting?

I don't like these zip locks.  I have the damnedest time taking a small sample for transfers (I am old and my hands shake) and the depth of the cup makes transferring a pita.  Anyone want about 30 cups?



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OfflineBobgas
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Tub just will not pin. 14 days. [Re: Bobgas]
    #28018772 - 10/27/22 07:03 AM (2 years, 2 months ago)

14 days and not a single pin. This morning I was also greeted with yellow myc piss.

I have no idea what else I ought to be doing other than waiting. Surface conditions look correct to my noob eyes. At what point do I start to consider abandoning the grow?



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OfflineDERRAYLD
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Re: Tub just will not pin. 14 days. [Re: Bobgas]
    #28018777 - 10/27/22 07:14 AM (2 years, 2 months ago)

Not sure you're being patient enough, some tubs take well over a month to fruit.

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OfflineRusty2096
rah rah raw in Lady gaga
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Registered: 08/23/22
Posts: 4,946
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Re: Tub just will not pin. 14 days. [Re: DERRAYLD]
    #28018866 - 10/27/22 08:10 AM (2 years, 2 months ago)

Quote:

DERRAYLD said:
Not sure you're being patient enough, some tubs take well over a month to fruit.



:whathesaid:

2 weeks is a very short term. What variety?


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Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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OfflineBobgas
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Re: Tub just will not pin. 14 days. [Re: Rusty2096]
    #28019324 - 10/27/22 12:36 PM (2 years, 2 months ago)

It's GT.

Got it- and fair enough. I haven't seen those times in other threads.


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OfflineBobgas
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Re: Tub just will not pin. 14 days. [Re: Bobgas]
    #28019332 - 10/27/22 12:39 PM (2 years, 2 months ago)

Another question. Checked in on my PF tek and found this pin!  I was letting it consolidate and thought it still had a couple more days.

What is the best think to do with this cake??  Based on what I read, I can birth it, funk it for a few mins, put it back in the jar, case it with coir and put a plastic baggie over it. Is that the right idea??  Should I do something else??

Thanks!!!  You folks are awesome for being as patient!



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πŸ…ƒπŸ„΄πŸ„°πŸ„Ό  πŸ„²πŸ„»πŸ„ΈπŸ„½ πŸ„ΆπŸ…†πŸ…πŸ„°πŸ„Ώ

Edited by Bobgas (10/27/22 01:04 PM)

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