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Offlinejbogart
Stranger

Registered: 10/01/22
Posts: 18
Last seen: 2 years, 22 days
Re: Spore germination at 100X magnification [Re: Rusty2096]
    #27982965 - 10/05/22 03:02 PM (2 years, 3 months ago)

Quote:

Rusty2096 said:
I don't think you can call that to overcome senescence. I see it more like humans having babies. The parent don't get any younger and still die to senescence (or McHeartAttacks)




Yeah I think you're right, if we're only talking about the monokaryon mate then it's kinda the same result as human babies as far as the genetics are concerned. Even if 'adult' dikaryon mycelia fused and exchanged nuclei they would still be the same age. Maybe if an old uncle Gary myc fused with a baby myc... Anyway it doesn't seem to explain senescence well.

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OfflineSymPlayTon
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Registered: 09/03/22
Posts: 420 420 Posts!
Loc: In a state of confusion
Last seen: 1 year, 3 months
Re: Spore germination at 100X magnification [Re: jbogart]
    #27983123 - 10/05/22 04:48 PM (2 years, 3 months ago)

Quote:

jbogart said:
Quote:

Rusty2096 said:
I don't think you can call that to overcome senescence. I see it more like humans having babies. The parent don't get any younger and still die to senescence (or McHeartAttacks)




Yeah I think you're right, if we're only talking about the monokaryon mate then it's kinda the same result as human babies as far as the genetics are concerned. Even if 'adult' dikaryon mycelia fused and exchanged nuclei they would still be the same age. Maybe if an old uncle Gary myc fused with a baby myc... Anyway it doesn't seem to explain senescence well.




I am wondering if how we culture in a lab if the issue of senescence will be a perpetual one.

From some of the papers I read when it goes dikaryotic it shares the DNA through the organism, and the entire organism will survive  if one of the DNA strands reach senescence, and that will make the larger organism open to more mating cycles. 

The heavy Isolation of the lab environment might not be conducive to this cycle.  Perhaps if we can make it a "ranching" cycle where we can continously feed and propogate a mature organism we would be able to benefit from a more robust mycelium mating program to maintain a larger organism. 

(All of this is thoughts and deductions from my minor understanding, and thrown together while sitting in a car ride)

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Offlinejbogart
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Registered: 10/01/22
Posts: 18
Last seen: 2 years, 22 days
Re: Spore germination at 100X magnification [Re: SymPlayTon]
    #27984834 - 10/06/22 03:05 PM (2 years, 3 months ago)

Quote:

SymPlayTon said:
I am wondering if how we culture in a lab if the issue of senescence will be a perpetual one.

From some of the papers I read when it goes dikaryotic it shares the DNA through the organism, and the entire organism will survive  if one of the DNA strands reach senescence, and that will make the larger organism open to more mating cycles. 

The heavy Isolation of the lab environment might not be conducive to this cycle.  Perhaps if we can make it a "ranching" cycle where we can continously feed and propogate a mature organism we would be able to benefit from a more robust mycelium mating program to maintain a larger organism. 

(All of this is thoughts and deductions from my minor understanding, and thrown together while sitting in a car ride)




I wasn't sure if you meant dikaryon mating - that would be an entirely different ball game. Would love to see an article on that.

I did find one article about arbuscular mycorrhizal fungi which describes multiple dikaryon mates over one lifetime. Then when they sporulate each spore might have one of 12 different nuclei. That is wild.

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Offlinejbogart
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Registered: 10/01/22
Posts: 18
Last seen: 2 years, 22 days
Re: Spore germination at 100X magnification [Re: jbogart]
    #28000767 - 10/15/22 06:06 PM (2 years, 3 months ago)

So I said I'd do a follow-up. It's fair to say the fungi had one over on me this time, but I think I managed to come out on top.

Both of the isolates I took from the plates originally turned out to be some kind of fungi - what I thought was a cubensis turned out to be Trich or some other green mold:



The brown clump just grew into a larger clump with a furry surface. No idea what kind of fungi/mold this is:



But I kept the (disgusting) original plate, and a week later something much more mushroomy emerged. I isolated four new plates of clean mycelium from here:



These have been growing out for nearly a week now. All are clean, three are showing tomentose growth but one has nice strong rhizomorphic growth which gives me courage:



And of the PF tek jars that were inoculated in parallel with the original contaminated plate, 6/10 actually look pretty great! Close to full colonisation after three weeks:



So whatever those brown spots were on the original plate obviously didn't do that well in the jar.
They were overwhelmed by the cubes before they even showed up.
Great job cubies :smile:

Lessons learnt...
  • I have no idea what I'm looking at under the microscope
  • Don't throw plates away until you know you're in the clear
  • Agar is awesome
  • My DP is a baby mold :thumbup:


I'm hoping to transfer the clean growth to some LC so I can go to grain and put down another batch of PF tek. Any advice is welcome! Thanks.

Edited by jbogart (10/15/22 06:09 PM)

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