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OfflineEnkidu
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Swabs to agar
    #27978165 - 10/02/22 02:56 PM (2 years, 3 months ago)

If you had 6 swabs gifted to you months back, 3 varieties, and you wanted to make agar plates today and swab them with your swabs, how many would you make?

Can you make 20 plates with that?

Assume you tried pulling off a old print and got nothing out of 20 plates last attempt


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Re: Swabs to agar [Re: Enkidu]
    #27978201 - 10/02/22 03:29 PM (2 years, 3 months ago)

So many numbers lol I suppose seems how it's 3 varieties I'd swab 3 plates, save 3 swabs to try incase they dont take.


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OfflineEnkidu
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Re: Swabs to agar [Re: rumfor69]
    #27978223 - 10/02/22 03:42 PM (2 years, 3 months ago)

Only 3?

You can't or shouldn't stretch them out to try and hit multiple plates?


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OfflineRusty2096
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Re: Swabs to agar [Re: Enkidu]
    #27978231 - 10/02/22 03:48 PM (2 years, 3 months ago)

Here is an easy way to do 2 plates per swab:

Cut the tip off. Stuff it in a plate. Use the rest to swab another plate

(I would also use only 3 like Rumfor said and save 3 for later in case - that magician is a wise one)


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Re: Swabs to agar [Re: Enkidu]
    #27978235 - 10/02/22 03:50 PM (2 years, 3 months ago)

I'd start 3 plates of each variety, giving you 9 starts.


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Re: Swabs to agar [Re: Enkidu]
    #27978236 - 10/02/22 03:51 PM (2 years, 3 months ago)

:wizard:


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OfflineEnkidu
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Re: Swabs to agar [Re: rumfor69]
    #27978250 - 10/02/22 04:04 PM (2 years, 3 months ago)

So like take one of each swab, cut out a bit and plant it in a plate, swab another plate with the leftover, make 6-9 plates from 3 swabs total, save 3 swabs for later ?


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OfflineJaksavage
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Re: Swabs to agar [Re: Enkidu] * 1
    #27978254 - 10/02/22 04:07 PM (2 years, 3 months ago)

Good plan.
Wet your swab with sterile h2o before you swab plate

:goodluck:


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OfflineEnkidu
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Re: Swabs to agar [Re: Jaksavage]
    #27978267 - 10/02/22 04:13 PM (2 years, 3 months ago)

So sterilize some water while I pc my agar?

I guess I'll just make 20 plates and then store the rest in the fridge


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Re: Swabs to agar [Re: Enkidu]
    #27978273 - 10/02/22 04:15 PM (2 years, 3 months ago)

Yes and yes.


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Re: Swabs to agar [Re: Jaksavage]
    #27978275 - 10/02/22 04:16 PM (2 years, 3 months ago)

Steril water can help for sure. I'm lazy so I do that as a last resort with the backup swabs lol

You can always just suran wrap extra plates and save for later like ya say. You'll need em for transfers anyhow with all the success you're about to have!


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OfflineEnkidu
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Re: Swabs to agar [Re: Jaksavage]
    #27978279 - 10/02/22 04:17 PM (2 years, 3 months ago)

Appreciate the good words and thank you fellers for all the help :hatsoff:


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OfflineEnkidu
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Re: Swabs to agar [Re: Enkidu]
    #27978326 - 10/02/22 04:44 PM (2 years, 3 months ago)

My apologies, I have more

I use to do 10g dme and 10g agar in 500ml water, will that work or do you need to alter your ratio for any reason?


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OfflineRusty2096
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Re: Swabs to agar [Re: Enkidu]
    #27978332 - 10/02/22 04:48 PM (2 years, 3 months ago)

It'll work.

Most people use LME and not DME.

Some people like softer plates for germination (maybe 7g agar/500ml) - I don't care about the softer plates as I use a loop and it does deposit water on my agar


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OfflineEnkidu
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Re: Swabs to agar [Re: Rusty2096]
    #27978336 - 10/02/22 04:50 PM (2 years, 3 months ago)

Thanks man, I'll have to get some lme to try :super:


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OfflineRusty2096
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Re: Swabs to agar [Re: Enkidu]
    #27978351 - 10/02/22 04:57 PM (2 years, 3 months ago)

Quote:

Enkidu said:
Thanks man, I'll have to get some lme to try :super:




Or just use grain water agar (GWA)

Cheap but most of all very accessible and no delivery time :super:



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OfflineEnkidu
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Re: Swabs to agar [Re: Enkidu]
    #27978356 - 10/02/22 05:00 PM (2 years, 3 months ago)

Grain water huh ? I'll have to look that up once I start prepping grain it sounds like


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OfflineRusty2096
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Re: Swabs to agar [Re: Enkidu]
    #27978358 - 10/02/22 05:03 PM (2 years, 3 months ago)

You don't even need to look it up.

Strain your grain water after cooking the grain.

Strain again through a coffee filter. Mix 500ml GW with 9g agar (or whatever quantity is your favorite) and PC for 15-30 min - I do 15 but most people here seem to think 30 is safer.


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OfflineEnkidu
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Re: Swabs to agar [Re: Rusty2096]
    #27978384 - 10/02/22 05:23 PM (2 years, 3 months ago)

Awesome, thanks man :super: I'll give it a shot once I get to that point

I have some prints too it looks like, I'll try hydrating them with some of this water and transfer to some agar and see if I can get any of those going


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Re: Swabs to agar [Re: Enkidu]
    #27978450 - 10/02/22 06:14 PM (2 years, 3 months ago)

Hydrate your swabs before swiping them.
For a print, use a loop or a flame sterile knife to scrape some spore print.

IMO

good luck


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OfflineRusty2096
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Re: Swabs to agar [Re: Jaksavage]
    #27978494 - 10/02/22 06:40 PM (2 years, 3 months ago)

Wait what?

When using a loop, I scrape the spores in a small amount of water, dip the loop in that water and then streak plates.

Am I alone doing it this way?


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OfflineEnkidu
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Re: Swabs to agar [Re: Rusty2096]
    #27978500 - 10/02/22 06:43 PM (2 years, 3 months ago)

Last time I tried to use prints I just scraped the print onto agar.

I made 20 plates with growth on 2 that didn't look promising, didn't even use it

I was planning to open the foil print, put water straight into the foil, mix around, then swab to some plates


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OfflineTormatoMFacebookDiscordReddit
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Re: Swabs to agar [Re: Rusty2096]
    #27978531 - 10/02/22 07:01 PM (2 years, 3 months ago)

Quote:

Rusty2096 said:
Wait what?

When using a loop, I scrape the spores in a small amount of water, dip the loop in that water and then streak plates.

Am I alone doing it this way?



That's definitely not how I do it. Flame sterilize metal loop, let it cool, swipe spore print then streak plate. No Water used. Water would just be a contam vector IMO.


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OfflineRusty2096
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Re: Swabs to agar [Re: Tormato]
    #27978541 - 10/02/22 07:07 PM (2 years, 3 months ago)

I sterilize the water in a very small condiment cup. Seems to work just fine for me.

That being said, I will def give it a try without the water next time to avoid the extra step that suddenly doesn't feel necessary anymore :shrug:


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Re: Swabs to agar [Re: Rusty2096]
    #27978555 - 10/02/22 07:15 PM (2 years, 3 months ago)

Always remember to K.I.S.S. :kiss:

:cheers:


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Re: Swabs to agar [Re: Tormato]
    #27978557 - 10/02/22 07:17 PM (2 years, 3 months ago)

Fair enough. Thank you :tongue2:


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OfflineEnkidu
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Re: Swabs to agar [Re: Rusty2096]
    #27978573 - 10/02/22 07:27 PM (2 years, 3 months ago)

So should I not be using water at all for my swabs too ?


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Re: Swabs to agar [Re: Enkidu]
    #27978793 - 10/02/22 09:34 PM (2 years, 3 months ago)

Quote:

Enkidu said:
So should I not be using water at all for my swabs too ?



I wouldn't


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Re: Swabs to agar [Re: Tormato]
    #27978800 - 10/02/22 09:37 PM (2 years, 3 months ago)

I just hydrated everything bro and put it to plates

Guess we'll find out how it works out

Maybe next weekend I'll do the same thing and leave out the water and use up another swab and print


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Re: Swabs to agar [Re: Enkidu]
    #27994394 - 10/11/22 08:31 PM (2 years, 3 months ago)

So 8 days later this is what I got so far



Nothing looks rhizo but it's coming from my injection point


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Re: Swabs to agar [Re: Enkidu]
    #27994404 - 10/11/22 08:36 PM (2 years, 3 months ago)

Dont worry about rhizo growth. It just needs to be clean, uniform, and organized. Often times germ plates won't throw rhizos till you transfer a couple times.


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Re: Swabs to agar [Re: rumfor69]
    #27994409 - 10/11/22 08:40 PM (2 years, 3 months ago)

You think this is mush myc?

Do you guys typically transfer quickly or let it grow out?

I probably won't have a good chance to do anything until Sunday


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Re: Swabs to agar [Re: Enkidu]
    #27994441 - 10/11/22 08:57 PM (2 years, 3 months ago)

Impossible to tell from those pics. I see uniform shapes and that's good at least. Typically you wanna transfer from germ plates fairly quickly before contams culture there too and take over if present. Basically if you can grab a rice sized piece that's had a chance to get away a little bit from your inoculation point then you should do so.


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Re: Swabs to agar [Re: rumfor69]
    #28036110 - 11/06/22 07:54 AM (2 years, 2 months ago)

I wanted to update this

So I ended up making transfers from those plates



Growth all looked clean so I took them to grain, this is some of the growth on the second transfers




And this is what the grain looks like now, innoced on 10/30, shook two days ago



So it seems like using the water worked alright for me

Last time I attempted to put spores to agar it failed and I didn't use water. Idr if I used swabs or exclusively prints or if the prints were dif


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