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bakedbeings
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
#27975136 - 09/30/22 09:19 AM (2 years, 3 months ago) |
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Quote:
echochild said:
Quote:
Bakedbeings said: all of them
Thank you! If I inoculate these premade jars in the future, should I keep the foil on until I see growth?the verm layer is the filter, the foil is just in the way. the jars need to breath
I'm highly considering going out and getting some vermiculite and taking the top layer off the other jars and re-sterilizing them in the pressure cooker again just in case. Think that will work? honestly if youre going to go to that trouble you should just remake the whole jar. theres no replacement for the assuredness that prepping from scratch gives you
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echochild
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Re: High Humidity in Monotub - is this an issue? [Re: bakedbeings]
#27976366 - 10/01/22 09:19 AM (2 years, 3 months ago) |
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Quote:
Bakedbeings said:
I'm highly considering going out and getting some vermiculite and taking the top layer off the other jars and re-sterilizing them in the pressure cooker again just in case. Think that will work? honestly if youre going to go to that trouble you should just remake the whole jar. theres no replacement for the assuredness that prepping from scratch gives you
Very true, and it would give me some good practice working on that for the future. I can also just dump the jars, clean them out, and reuse them. I just hate wasting ingredients, but you make a very good point.
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echochild
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
#27976457 - 10/01/22 10:26 AM (2 years, 3 months ago) |
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Ok, so I'm pretty sure Tub 2 is pinning right?
I have FAE with polyfil. Is this the point where I should be cracking the tub lid or wait until they are bigger?
Edit: Or is the PAE from the polyfil enough?
Edited by echochild (10/01/22 10:28 AM)
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bakedbeings
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
#27976465 - 10/01/22 10:39 AM (2 years, 3 months ago) |
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i say let it ride
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echochild
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Re: High Humidity in Monotub - is this an issue? [Re: bakedbeings]
#27977224 - 10/01/22 08:51 PM (2 years, 3 months ago) |
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I decided to open it up because I thought I saw something fuzzy in the corner but didn't have a good view on it.
So to show the current progress vs the last time people said Tub 2 was iffy:
Earlier Tub 2 shot: 
Shots from today:

I know I'm being a nervous nelly. Can't help it with my first babies
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bakedbeings
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
#27977254 - 10/01/22 09:23 PM (2 years, 3 months ago) |
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looks promising
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echochild
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Re: High Humidity in Monotub - is this an issue? [Re: bakedbeings]
#27977256 - 10/01/22 09:24 PM (2 years, 3 months ago) |
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Quote:
Bakedbeings said: looks promising
Thanks Baked 
What's up with that fuzzy stuff in the lower left of the first pic from the shots I took today?
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Rusty2096
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
#27977259 - 10/01/22 09:31 PM (2 years, 3 months ago) |
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Any chance you could take a pic with focus on that bottom left spot?
Could be cobweb, could be nothing - hard to tell (at least for me) from that pic.
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!.
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echochild
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Re: High Humidity in Monotub - is this an issue? [Re: Rusty2096]
#27977263 - 10/01/22 09:33 PM (2 years, 3 months ago) |
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Quote:
Rusty2096 said: Any chance you could take a pic with focus on that bottom left spot?
Could be cobweb, could be nothing - hard to tell (at least for me) from that pic.
Bottom left of which pic? (I know what the orientation of the tub is, so that would help me reorient the pic).
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Rusty2096
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
#27977279 - 10/01/22 09:49 PM (2 years, 3 months ago) |
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Quote:
echochild said:
Quote:
Bakedbeings said: looks promising
Thanks Baked 
What's up with that fuzzy stuff in the lower left of the first pic from the shots I took today?
That one
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!.
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echochild
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Re: High Humidity in Monotub - is this an issue? [Re: Rusty2096]
#27977296 - 10/01/22 10:06 PM (2 years, 3 months ago) |
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Rusty2096
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
#27977304 - 10/01/22 10:18 PM (2 years, 3 months ago) |
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Yeah that's not good.
How fast is it growing?
On the pics, it looks like it's killing the pins that were in there? Can you confirm that from what you see in person?
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!.
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Semi-solid liquid culture (SSLC)
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echochild
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Re: High Humidity in Monotub - is this an issue? [Re: Rusty2096]
#27977308 - 10/01/22 10:27 PM (2 years, 3 months ago) |
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Quote:
Rusty2096 said: Yeah that's not good.
How fast is it growing?
On the pics, it looks like it's killing the pins that were in there? Can you confirm that from what you see in person?
I just noticed it today, so I can't tell you how fast it's moving.
There are a lot of little pins in the fuzzy area, I don't know how to identify if they are being killed. What would that look like?
I did notice, pretty much all of the bigger pins you can clearly see are fuzzy at the bottoms.
Edited by echochild (10/01/22 10:28 PM)
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Rusty2096
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
#27977314 - 10/01/22 10:36 PM (2 years, 3 months ago) |
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The fuzzy feet on the pins is quite normal. Could be genetics, could be too little FAE but it's early to tell.
If they get real fuzzy in a day or 2 you might want to increase FAE.
In the mean time, I suggest you keep a close eye on that gray fuzzy zone and monitor how fast it grows. If it does grow, there won't be much you can do beside praying your flush comes in before the tub is overtaken.
You can tell pins are dying/stopped growing (they are called aborts) from the sometimes darker caps but mostly from the fact that they stopped growing (there may be other ways to tell).
Edit: many factors can cause aborts: contamination, sub drying out, improper surface conditions, too little FAE, etc.
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!.
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Edited by Rusty2096 (10/01/22 10:40 PM)
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echochild
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Re: High Humidity in Monotub - is this an issue? [Re: Rusty2096]
#27977321 - 10/01/22 10:42 PM (2 years, 3 months ago) |
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Fun, fun... Thanks for the advice 
Well if this tub goes down that will be the final lesson in don't do MSS to Grain. Thank fully I am on an agar T4 now with another strain that I'm considering getting into a bag when I can get myself an impulse sealer.
If it is cobweb, and is advancing, should I just pick the larger pins and call it a day with this tub?
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Rusty2096
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
#27977328 - 10/01/22 10:48 PM (2 years, 3 months ago) |
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Not sure, never had that kind on contam. I'm a bacteria pro and this may be mold.
I'd let it ride and try harvest the most I can, picking wisely. Maybe someone else can chime in here.
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!.
We don't own things - things own us.
Semi-solid liquid culture (SSLC)
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bakedbeings
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
#27977359 - 10/01/22 11:29 PM (2 years, 3 months ago) |
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in my limited experience with cobweb it seemed like increasing FAE slowed it down and ive read but dont know for sure that its the only mold susceptible to peroxide. you can try opening up the holes near that corner and give it a spray if you want
Quote:
echochild said: Fun, fun... Thanks for the advice 
Well if this tub goes down that will be the final lesson in don't do MSS to Grain. forreal Thank fully I am on an agar T4 now with another strain that I'm considering getting into a bag when I can get myself an impulse sealer.
If it is cobweb, and is advancing, should I just pick the larger pins and call it a day with this tub? you can play it by ear...if it looks like everythings about to be covered yeah pick everybody and move on
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echochild
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Re: High Humidity in Monotub - is this an issue? [Re: bakedbeings]
#27977365 - 10/01/22 11:41 PM (2 years, 3 months ago) |
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I'll check and see if it is advancing tomorrow. If it is, I'll open up the FAE and mist it with some 3% H2O2 and see what happens.
Aside question: This was one of the two strains I never made back up agar for, because it was from before I started working with agar.
Do you think it would be possible to take an immature pin, and try to clone it via agar? Or does that only work with mature mushrooms?
Edited by echochild (10/01/22 11:42 PM)
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hazyhorse
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
#27977370 - 10/01/22 11:51 PM (2 years, 3 months ago) |
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you can clone a mushroom from a pin but itโs best to take inner tissue from the mushroom bc inner tissue is inherently sterile, it can be hard to do that from a pin. ripping the mushroom in half like a cheese stick is best for mature mushrooms so depends on the size of the pin
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echochild
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Re: High Humidity in Monotub - is this an issue? [Re: hazyhorse]
#27977376 - 10/01/22 11:57 PM (2 years, 3 months ago) |
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Quote:
hazyhorse said: you can clone a mushroom from a pin but itโs best to take inner tissue from the mushroom bc inner tissue is inherently sterile, it can be hard to do that from a pin. ripping the mushroom in half like a cheese stick is best for mature mushrooms so depends on the size of the pin
Well, there is a fatty pin in there. It's about 4 mm across on the stem, and I have steady enough hands that I have been doing 2 mm agar transfers. Maybe if it gets to about 6 mm across, I'm pretty sure I could split it and get a small 2 mm sample.
Do you think that will work?
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