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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] 1
#28022773 - 10/29/22 12:08 PM (1 year, 2 months ago) |
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Quote:
p9hu7 said: Should the standard cycle be sufficient to sterilize each load, I have no reason at this point to believe that the cycle will fail at this point, I think it will be interesting and beneficial to first determine the minimum cycle required to sterilize a 1L grain master rather than move onto grain bag's, initially.
Reducing the cycle by 30 mins to begin. I will choose only one grain for this as the cycle appears to have the same outcome despite grain choice (should all the grains continue to pass). I will also use 4 x 1L jars/ cycle (same grain)to determine the efficiency, perhaps some will sterilize while others fail.
1 hour at 15psi with a 10 mins vent. If the cycle is verified at 1 hour I will drop by 10 mins until it fails.
If the cycle fails I will increase by 10 minute increments until verification is achieved.
We will then have the minimum parameter for cycle effectiveness.
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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bakedbeings
orbiter of truth


Registered: 09/01/20
Posts: 4,218
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Re: Autoclave Validation and Load/Cycle Verification [Re: SirPsycho] 1
#28022780 - 10/29/22 12:20 PM (1 year, 2 months ago) |
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Quote:
SirPsycho said: Are you planning on doing ampoules for each jar?
We need to get a GoFundMe together, this man dropping serious coin on this experiment.
i wired him 7k. p9 that was you right?
-------------------- 🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
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Re: Autoclave Validation and Load/Cycle Verification [Re: bakedbeings]
#28022782 - 10/29/22 12:24 PM (1 year, 2 months ago) |
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Quote:
bakedbeings said:
Quote:
SirPsycho said: Are you planning on doing ampoules for each jar?
We need to get a GoFundMe together, this man dropping serious coin on this experiment.
i wired him 7k. p9 that was you right?
Colombian pesos? That's 1.46USD. You are very generous and supportive
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: SirPsycho]
#28022791 - 10/29/22 12:39 PM (1 year, 2 months ago) |
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Quote:
SirPsycho said: Are you planning on doing ampoules for each jar?
Yeah, I think that one amp is too small of a sample group when hunting for the low threshold.
Edited by Stipe-n Cap (10/29/22 03:17 PM)
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hazyhorse
scoobin



Registered: 03/19/19
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Re: Autoclave Validation and Load/Cycle Verification [Re: SirPsycho]
#28022895 - 10/29/22 02:08 PM (1 year, 2 months ago) |
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Quote:
SirPsycho said: We need to get a GoFundMe together, this man dropping serious coin on this experiment.
dude i would gladly contribute some money to this, p9 is a real one for running all these tests. just let me know where to drop the doge coin
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Re: Autoclave Validation and Load/Cycle Verification [Re: SirPsycho] 1
#28023013 - 10/29/22 03:12 PM (1 year, 2 months ago) |
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Quote:
SirPsycho said: We need to get a GoFundMe together, this man dropping serious coin on this experiment.
Patreon
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: Tormato] 1
#28023065 - 10/29/22 03:45 PM (1 year, 2 months ago) |
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Millet verified and added to the OP.
This is starting to get boring, lets spice shit up with with a new, younger pc
Edited by Stipe-n Cap (10/29/22 04:12 PM)
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap]
#28023153 - 10/29/22 04:34 PM (1 year, 2 months ago) |
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Quote:
p9hu7 said: Millet verified and added to the OP.
This is starting to get boring, lets spice shit up with with a new, younger pc

-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] 1
#28023307 - 10/29/22 06:16 PM (1 year, 2 months ago) |
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Quote:
p9hu7 said: lets spice shit up with with a new, younger pc
How does the wife feel about that?
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
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Re: Autoclave Validation and Load/Cycle Verification [Re: SirPsycho]
#28023351 - 10/29/22 06:34 PM (1 year, 2 months ago) |
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Quote:
SirPsycho said:
Quote:
p9hu7 said: lets spice shit up with with a new, younger pc
How does the wife feel about that?
Pretty sure he took it away from her for her own safety
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: Rusty2096] 3
#28050132 - 11/14/22 07:33 AM (1 year, 2 months ago) |
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Here we go folks, the one and only....

Let's see how this bad boy holds up to scientific inquiry, I'd honestly rather a result that I'm not expecting to make this a little more interesting. I'll keep an open mid while running this test, we will all benefit from these results either way so standby to standby.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap]
#28050133 - 11/14/22 07:35 AM (1 year, 2 months ago) |
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I think you got the wrong one though. IIRC the IP gang was saying only the IP Max can be vented
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] 1
#28050138 - 11/14/22 07:39 AM (1 year, 2 months ago) |
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Can't wait, you're just awesome 
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: 🌌
Last seen: 3 days, 21 hours
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Re: Autoclave Validation and Load/Cycle Verification [Re: SirPsycho]
#28050140 - 11/14/22 07:40 AM (1 year, 2 months ago) |
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Quote:
SirPsycho said:

I think you got the wrong one though. IIRC the IP gang was saying only the IP Max can be vented
So other IPs are bombs?
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: SirPsycho] 1
#28050141 - 11/14/22 07:40 AM (1 year, 2 months ago) |
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Quote:
SirPsycho said:

I think you got the wrong one though. IIRC the IP gang was saying only the IP Max can be vented
No way those folks are running 400+$ steamers, this is the best ol Stipes pocket can handle atm 
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,901
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] 1
#28050144 - 11/14/22 07:42 AM (1 year, 2 months ago) |
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Well maybe if you didn't get that fancy bumper for your Suzuki
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
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Re: Autoclave Validation and Load/Cycle Verification [Re: SirPsycho]
#28050146 - 11/14/22 07:44 AM (1 year, 2 months ago) |
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Did you also buy that electric knife or is it just a by sander?
I wondered if they could be used to harvest a canopy really fast. Some people use those to filet fish so I bet they can handle mushrooms.
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: Rusty2096] 2
#28050162 - 11/14/22 07:55 AM (1 year, 2 months ago) |
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I'm pretty sure that this is the model used in for the testing with liquid media linked in the OP; no I didn't buy the electric knife and yes it can be used to harvest canopies
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bakedbeings
orbiter of truth


Registered: 09/01/20
Posts: 4,218
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap]
#28050204 - 11/14/22 08:33 AM (1 year, 2 months ago) |
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Quote:
I wondered if they could be used to harvest a canopy really fast
this is the future of speed harvesting
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: bakedbeings] 1
#28050288 - 11/14/22 09:22 AM (1 year, 2 months ago) |
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The last of the grain to be verified
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