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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load Penetration [Re: SirPsycho] 1
#28019029 - 10/27/22 09:57 AM (1 year, 3 months ago) |
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I'll look around to see if I can find a cheap one second hand otherwise it will have to wait; until then, for the folks reading, the instant pot has already been run through the verification process by a trained virologist who runs an HIV lab.
The IP failed verification using the exact same procedure in the OP with a couple of differences:
1. The grain was loaded into pint jars;
2. Th IP was run for 4 hours;
The IP failed to penetrate the load even with a 4 hour cycle.
For those who want to argue about equipment, microbiology, etc etc, they can read this reply I made here:
https://www.shroomery.org/forums/showflat.php/Number/28018916#28018916
The same applies to instant pots, open air inoculations, and this guy:

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Screwup
Googles your dumb questions


Registered: 01/27/22
Posts: 6,296
Last seen: 7 hours, 12 minutes
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] 1
#28019040 - 10/27/22 10:06 AM (1 year, 3 months ago) |
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Quote:
p9hu7 said: I'll look around to see if I can find a cheap one second hand otherwise it will have to wait; until then, for the folks reading, the instant pot has already been run through the verification process by a trained virologist who runs an HIV lab.
The IP failed verification using the exact same procedure in the OP with a couple of differences:
1. The grain was loaded into pint jars;
2. Th IP was run for 4 hours;
The IP failed to penetrate the load even with a 4 hour cycle.
For those who want to argue about equipment, microbiology, etc etc, they can read this reply I made here:
https://www.shroomery.org/forums/showflat.php/Number/28018916#28018916
The same applies to instant pots, open air inoculations, and this guy:

Where did you get this photo of me??? Please delete it.
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bakedbeings
orbiter of truth


Registered: 09/01/20
Posts: 4,218
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
#28019047 - 10/27/22 10:12 AM (1 year, 3 months ago) |
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Quote:
Mush Cult is one of the few hobby communities where folks line up to receive trophies for a job barely done.
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Egon_Spengler
Ever lurkin never workin



Registered: 04/02/22
Posts: 2,294
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
#28019048 - 10/27/22 10:13 AM (1 year, 3 months ago) |
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Quote:
p9hu7 said:
https://www.shroomery.org/forums/showflat.php/Number/28018916#28018916
The same applies to instant pots, open air inoculations, and this guy:

This guy, lol.
Your response linked above is great because it highlights an important distinction often overlooked on the forums: that most of us are not mycologists, most of us are at best hobbyists. Any gardener can grow tomatoes, this does not make them a botanist, nor a horticulturalist.
Edited by Egon_Spengler (10/27/22 10:25 AM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load Penetration [Re: Egon_Spengler] 2
#28019840 - 10/27/22 05:13 PM (1 year, 2 months ago) |
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Now 2 hours into hydrating this corn:

It appears to be plump and hydrated on the surface but is at best 50%
Not cheap either

Corn can officially fuck right off
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B Traven
Stranger



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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] 1
#28019882 - 10/27/22 05:41 PM (1 year, 2 months ago) |
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Just to be clear, what's the beef with instant pots?
I wouldn't trust them for grain, but have been satisfied using one for agar plates.
Is that also seen as unreliable?
-------------------- Beware of advice- even this.
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Rusty2096
rah rah raw in Lady gaga



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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
#28019887 - 10/27/22 05:43 PM (1 year, 2 months ago) |
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No it's not. It works for liquids.
But who said instapot?

-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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B Traven
Stranger



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Re: Autoclave Validation and Load Penetration [Re: Rusty2096]
#28019890 - 10/27/22 05:46 PM (1 year, 2 months ago) |
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Quote:
Rusty2096 said: No it's not. It works for liquids.
But who said instapot?
 
Lol
P9hu7 mentioned that his eloquent diss track also applied to them.
-------------------- Beware of advice- even this.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,901
Loc: Rent free in your head
Last seen: 2 hours, 49 minutes
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Re: Autoclave Validation and Load Penetration [Re: B Traven] 1
#28019898 - 10/27/22 05:49 PM (1 year, 2 months ago) |
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Quote:
B Traven said:
Quote:
Rusty2096 said: No it's not. It works for liquids.
But who said instapot?
 
Lol
P9hu7 mentioned that his eloquent diss track also applied to them.
Don't worry, I'm the one Rusty's getting his panties all bunched up over
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: B Traven]
#28019909 - 10/27/22 05:53 PM (1 year, 2 months ago) |
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Quote:
B Traven said: Just to be clear, what's the beef with instant pots?
I wouldn't trust them for grain, but have been satisfied using one for agar plates.
Is that also seen as unreliable?
The IP underwent cycle verification for liquids here:
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0208769
Gtg for liquid media.
The problem with the IP is that it's not designed nor rated for use as an autoclave, neithe is the 23q presto for that matter; however, despite this fact the 23q presto can be utilized as an autoclave, the electeic kitchen appliance cannot.
I am supremely confident due to the fact that the IP has been put through the verification process for grains and has failed miserably.
I have proven that the 23q Presto can be utilized as an autoclave by validating it using the appropriate verification process.
The IP is not a suitable autoclave, the IP has been tested and confirmed for use with liquids.
I do not want this thread to become an IP argument thread though.
Edit:
Quote:
p9hu7 said: The instant pot has already been run through the verification process by a trained virologist who runs an HIV lab.
The IP failed verification using the exact same procedure in the OP with a couple of differences:
1. The grain was loaded into pint jars;
2. Th IP was run for 4 hours;
The IP failed to penetrate the load even with a 4 hour cycle.
Edited by Stipe-n Cap (10/28/22 09:06 PM)
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B Traven
Stranger



Registered: 03/10/20
Posts: 2,479
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
#28020052 - 10/27/22 07:05 PM (1 year, 2 months ago) |
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Quote:
p9hu7 said:
Quote:
B Traven said: Just to be clear, what's the beef with instant pots?
I wouldn't trust them for grain, but have been satisfied using one for agar plates.
Is that also seen as unreliable?
The IP underwent cycle verification for liquids here:
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0208769
Gtg for liquid media.
The problem with the IP is that it's not designed nor rated for use as an autoclave, neithe is the 23q presto for that matter; however, despite this fact the 23q presto can be utilized as an autoclave, the electeic kitchen appliance cannot.
I am supremely confident due to the fact that the IP has been put through the verification process for grains and has failed miserably.
I have proven that the 23q Presto can be utilized as an autoclave by validating it using the appropriate verification process.
The IP is not a suitable autoclave, the IP has been tested and confirmed for use with liquids.
I do not want this thread to become an IP argument thread though.
Edit:
Quote:
p9hu7 said: The instant pot has already been run through the verification process by a trained virologist who runs an HIV lab.
The IP failed verification using the exact same procedure in the OP with a couple of differences:
1. The grain was loaded into pint jars;
2. Th IP was run for 4 hours;
The IP failed to penetrate the load even with a 4 hour cycle.
Cool, thanks for the explanation, I was definitely more just curious whether ip use for agar was contraindicated. I pretty much chalk up all my contamination issues at this point to mediocre SAB technique or random catastrophic lid failures, but there's still plenty of room for me to miss vectors. As this is the most science-y treatment of sterilization parameters I've seen, I figured I'd ask. I used to do agar in the prestos, but then got lazy.
-------------------- Beware of advice- even this.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load Penetration [Re: B Traven] 2
#28020138 - 10/27/22 07:35 PM (1 year, 2 months ago) |
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Any liquid media should be gtg in the intant pot, seems like an easy/convenient device for liquids.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap]
#28020397 - 10/27/22 09:18 PM (1 year, 2 months ago) |
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Fucking hell, I looked away from the pc for a min while chatting on our discord server and the guage hit 16psi while I was looking at my phone. The max registering thermometer need only exceed the 15psi for a second to fuck up the reading so I'm running it again this evening, lol.
This time I will be babysitting this thing
Edited by Stipe-n Cap (10/28/22 09:06 PM)
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Land Trout
Stranger



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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
#28020424 - 10/27/22 09:35 PM (1 year, 2 months ago) |
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: Land Trout]
#28022089 - 10/28/22 09:05 PM (1 year, 2 months ago) |
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Cycle has been successfully verified for corn, results/video added to the OP.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap]
#28022099 - 10/28/22 09:11 PM (1 year, 2 months ago) |
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The only way I was able to fully hydrate popcorn was to PC it. I would PC the corn to hydrate for like 20 min and then I would strain and load it into the jars.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: Pastywhyte]
#28022102 - 10/28/22 09:12 PM (1 year, 2 months ago) |
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Makes sense, that's what I'm hearing from the folks who run corn in their instant pots.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] 4
#28022684 - 10/29/22 11:07 AM (1 year, 2 months ago) |
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Should the standard cycle be sufficient to sterilize each load, I have no reason at this point to believe that the cycle will fail at this point, I think it will be interesting and beneficial to first determine the minimum cycle required to sterilize a 1L grain master rather than move onto grain bag's, initially.
Reducing the cycle by 30 mins to begin. I will choose only one grain for this as the cycle appears to have the same outcome despite grain choice (should all the grains continue to pass). I will also use 4 x 1L jars/ cycle (same grain)to determine the efficiency, perhaps some will sterilize while others fail.
1 hour at 15psi with a 10 mins vent. If the cycle is verified at 1 hour I will drop by 10 mins until it fails.
If the cycle fails I will increase by 10 minute increments until verification is achieved.
We will then have the minimum parameter for cycle effectiveness.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap]
#28022755 - 10/29/22 11:50 AM (1 year, 2 months ago) |
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π―
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,901
Loc: Rent free in your head
Last seen: 2 hours, 49 minutes
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Re: Autoclave Validation and Load/Cycle Verification [Re: rumfor69]
#28022771 - 10/29/22 12:05 PM (1 year, 2 months ago) |
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Are you planning on doing ampoules for each jar?
We need to get a GoFundMe together, this man dropping serious coin on this experiment.
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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    "Mist your balls and fan your asshole" - Pandaskis, 2023
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