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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] 1
#28007191 - 10/19/22 09:53 PM (1 year, 3 months ago) |
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To my fellow 'Standard' measurement users, 120C is 248F. I knew this off the top of my head and did not use Google for the conversion (definitely had to use Google..).
Forgive my noobness but what is considered to be the 'minimum' core temperature for proper sterilization?
Thanks again for running this experiment and I'm glad the incubator situation is getting sorted!
--------------------
I 5318008 NOT a virgin!
Edited by bigfootscreepyuncl (10/19/22 10:21 PM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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15 psi is 121°C, that's the benchmark.
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GandalfTheWhite
Newb


Registered: 05/02/21
Posts: 84
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] 2
#28007234 - 10/19/22 11:07 PM (1 year, 3 months ago) |
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This is going to be a killer thread once it all gets going. Thank you for this! Be interesting to see how the different grain densitys come into play.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Autoclave Validation and Load Penetration [Re: GandalfTheWhite] 1
#28007264 - 10/19/22 11:53 PM (1 year, 3 months ago) |
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Those Amazonian bastards
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load Penetration [Re: Pastywhyte] 3
#28007456 - 10/20/22 06:36 AM (1 year, 3 months ago) |
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I'll be putting this on hold until the incubator arrives.
Although the thermometer registered 120° as the max temp achieved it doesn't tell me for how long, sterilizing a paper chem strip is one thing, an ampoule of spores is quite another, so...
Results are very preliminary atm but will have the BI' running by next week, I hope.
In the meantime I'll go buy some oats and corn so that everything is ready when the unit comes in.
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,956
Loc: Hyperspace Chicken Coop
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] 1
#28007673 - 10/20/22 10:22 AM (1 year, 3 months ago) |
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Just a though: the thermometer sticking out of the grain jar like this means that a good portion of it is directly exposed to steam. I assume that way the thermometer can transfer heat from outside the jar to the center of the grain just by heating up to target temperature earlier on the exposed side.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load Penetration [Re: Baba Yaga] 2
#28007710 - 10/20/22 10:44 AM (1 year, 3 months ago) |
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I'm not entirely sure how significant that effect will be, I will run the thermometer again when I cycle the BI. I just read in the manual that the thermometer must be standing at a 45° angle and not vertically; the thermometer alone isn't accurate enough on it's own for many reasons, this is why I'm relying upon 3 separate line of inquiry as apposed to just the one.
I assume that because the mercury sits at the base of the thermometer that it's design doesn't allow for much transfer from one end to the other, the BI is the benchmark and has the last say in the matter.
I may have acquired a false reading by standing vertically so it will be easy to run again once the incubator arrives.
Next time I'll tilt the jar at a 45, no way to prevent the tip from protruding from the jar if I want to maintin the sane volume of grain/test a representative load.
Edited by Stipe-n Cap (10/20/22 11:13 AM)
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,956
Loc: Hyperspace Chicken Coop
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
#28007846 - 10/20/22 01:22 PM (1 year, 3 months ago) |
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You are absolutely right, the bio indicator will deliver the prove of sterilization.
The thermometer is great to verify the temp of the PC if run alongside the test jars since the pressure gauges are usually not that accurate especially after a good deal of use. What it won't do though is giving a useful measurement of the core temperature if it is exposed like this. The entire stainless steel housing of the thermometer will heat up through conduction faster than heat penetration through grain.
Don't want to discredit your efforts or nitpick just contributing what I think is a valid point.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load Penetration [Re: Baba Yaga] 1
#28007897 - 10/20/22 01:59 PM (1 year, 3 months ago) |
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Definitely a valid point; the metal housing can be removed but I'll hit up some stores to see if I can find a jar with a similar circumference but with a more appropriate length.
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bakedbeings
orbiter of truth


Registered: 09/01/20
Posts: 4,218
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
#28007927 - 10/20/22 02:33 PM (1 year, 3 months ago) |
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Quote:
Next time I'll tilt the jar at a 45, no way to prevent the tip from protruding from the jar if I want to maintin the sane volume of grain/test a representative load.
is there anything wrong with putting both devices in the same jar?
-------------------- 🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load Penetration [Re: bakedbeings]
#28007932 - 10/20/22 02:36 PM (1 year, 3 months ago) |
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Not enough room for both.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
#28007936 - 10/20/22 02:40 PM (1 year, 3 months ago) |
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awesome
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load Penetration [Re: Baba Yaga]
#28008036 - 10/20/22 03:50 PM (1 year, 3 months ago) |
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So I was just speaking to some lab techs, they said that the protruding end won't have too much of an effect as the mercury is situated at the bottom; however, it is an emersion thermometer, so it's intended to be emersed.
This is my intended fix:

The taller bottle has the same circumference so the steam penetration will be comparable.
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: 🌌
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
#28008048 - 10/20/22 04:01 PM (1 year, 3 months ago) |
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Yo p9, how's that Hawkin classic treating you? I assume not so bad and no blowing up at least since you are still active here
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load Penetration [Re: Rusty2096]
#28008062 - 10/20/22 04:09 PM (1 year, 3 months ago) |
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Makes excellent food 👌
The gf almost killed herself a couple of times, had to keep telling her "no you cant take the lid off to make it cool faster" lel
Maybe not kill but definitely scald to the point of wishing for death
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: 🌌
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
#28008067 - 10/20/22 04:10 PM (1 year, 3 months ago) |
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Edit: It's actually idiot proof. You simply can't open it as long as it's under pressure (you GF is safe - and I do not mean you GF is an idiot - this is NOT sarcasm. Fuck why do I feel I'm getting myself in trouble.)
POST DELETED BY RUSTY. REASON: FEAR OR BEING MISUNDERSTOOD AND LAPIDATED
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
Edited by Rusty2096 (10/20/22 04:14 PM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load Penetration [Re: Rusty2096]
#28008078 - 10/20/22 04:18 PM (1 year, 3 months ago) |
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Lol, she's not the sharpest...as far as the lid goes it doesn't lock, it just latches on.

Just squeeze the handle slightly and unlatch.
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: 🌌
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] 1
#28008088 - 10/20/22 04:22 PM (1 year, 3 months ago) |
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Quote:
p9hu7 said: Lol, she's not the sharpest...
Edit: Neither are you 
Read the manual. Or just trust me (I don't need you're full trust, just a bit). Unlatch it before it's fully depressurized. It wont open. Force it, still won't open. Look at how it's engineered. The lid pushes itself against the ..(fuck I don't know that word in English). Anyhow, you get the point 
PS: just RTFM
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
Edited by Rusty2096 (10/20/22 04:23 PM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load Penetration [Re: Rusty2096]
#28008091 - 10/20/22 04:24 PM (1 year, 3 months ago) |
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Ah, yeah I guess that makes sense, trust activated ; )
Still not going to try to pry the lid open under pressure though, I don't trust the engineering skills of the folks that design flimsy equipment.
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: 🌌
Last seen: 3 days, 21 hours
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
#28008092 - 10/20/22 04:24 PM (1 year, 3 months ago) |
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-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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