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InvisibleStipe-n CapMDiscord
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Re: Autoclave Validation and Load Penetration [Re: Tormato] * 1
    #27975275 - 09/30/22 11:57 AM (1 year, 3 months ago)

The waiting.....



The rest of the gear should have arrived by then.  :waitingpatiently:


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Invisiblemind.at.large
Myconerd


Registered: 12/13/16
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
    #27975345 - 09/30/22 01:13 PM (1 year, 3 months ago)

I would love to see you run this with different density substrates, whether spawn bags or sawdust bags, etc. I mean I know this is a brand new experiment so Iโ€™m not trying to put more on you haha but it could be useful for sure.

Thanks for all ya do!


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Mind's Easy Bag 2 Bag Grain Transfers


Endless Sub Tek


...the doll's trying to kill me and the toaster's been laughing at me...


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InvisibleStipe-n CapMDiscord
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Re: Autoclave Validation and Load Penetration [Re: mind.at.large]
    #27975352 - 09/30/22 01:19 PM (1 year, 3 months ago)

I'm starting with grain jars as a baseline, depending upon the results (if the load successfully sterilizes) I will move onto spawn bags and any other common load we subject our sterilizers to with the exception of liquids.

If grain jars prove impossible to verify then it would be pointless to move up to a load with greater density; Liquids will be exluded due to the ease with which they are sterilized.


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Myconerd


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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
    #27975354 - 09/30/22 01:21 PM (1 year, 3 months ago)

Good point. Excited to see results


--------------------
Mind's Easy Bag 2 Bag Grain Transfers


Endless Sub Tek


...the doll's trying to kill me and the toaster's been laughing at me...


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InvisibleEgon_Spengler
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Re: Autoclave Validation and Load Penetration [Re: mind.at.large]
    #27981292 - 10/04/22 02:17 PM (1 year, 3 months ago)

:popcorn:


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InvisibleStipe-n CapMDiscord
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Re: Autoclave Validation and Load Penetration [Re: Egon_Spengler] * 2
    #27990017 - 10/09/22 11:28 AM (1 year, 3 months ago)

Going to run this 6.5L cheapo as well:



If nothing else it should be great for liquids.

Bought it for some Canadian Thanksgiving oxtail :cool:


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OfflineRusty2096
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
    #27990031 - 10/09/22 11:33 AM (1 year, 3 months ago)

Quote:

p9hu7 said:
Going to run this 6.5L cheapo as well:



If nothing else it should be great for liquids.

Bought it for some Canadian Thanksgiving oxtail :cool:




I have one of those. Besides being small, it works really well. Very useful when I don't want to use the big 23qt. Perfect to PC agar media bottles and LC jars. Or small batch of grains (you can fit 1x1qt + 2x0.5qt jars in there).


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)


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InvisibleStipe-n CapMDiscord
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Re: Autoclave Validation and Load Penetration [Re: Rusty2096]
    #27990039 - 10/09/22 11:35 AM (1 year, 3 months ago)

Nice, it feels flimsy so good to know someone here has successfully run it without killing themselves :lol:


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OfflineRusty2096
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
    #27990046 - 10/09/22 11:39 AM (1 year, 3 months ago)

Quote:

p9hu7 said:
Nice, it feels flimsy so good to know someone here has successfully run it without killing themselves :lol:




:lol:

I bet it'll surprise you (even for grain) - and yes, you're safe (but I would NOT add weights on the vent weight of this one)


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)


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Invisiblebigfootscreepyuncl
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Re: Autoclave Validation and Load Penetration [Re: Rusty2096] * 1
    #27990062 - 10/09/22 11:49 AM (1 year, 3 months ago)

Goddamn I love reading your writeups :popcorn:

Another invaluable contribution to add to the archives :rockon:


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I :heart: 5318008


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InvisibleStipe-n CapMDiscord
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Re: Autoclave Validation and Load Penetration [Re: bigfootscreepyuncl]
    #27990077 - 10/09/22 11:56 AM (1 year, 3 months ago)

Thanks, man, I appreciate it.


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InvisibleStipe-n CapMDiscord
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] * 2
    #27993782 - 10/11/22 01:40 PM (1 year, 3 months ago)

Almost ready, just waiting on the thermometer.



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Invisiblemind.at.large
Myconerd


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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
    #27993834 - 10/11/22 02:29 PM (1 year, 3 months ago)

:frankensmoke:


--------------------
Mind's Easy Bag 2 Bag Grain Transfers


Endless Sub Tek


...the doll's trying to kill me and the toaster's been laughing at me...


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InvisibleStipe-n CapMDiscord
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Re: Autoclave Validation and Load Penetration [Re: mind.at.large] * 6
    #28004908 - 10/18/22 03:26 PM (1 year, 3 months ago)

Final piece of equipment has arrived today, will begin work in the next day or two.



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OfflineRotnpins
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] * 1
    #28004911 - 10/18/22 03:26 PM (1 year, 3 months ago)

:justcantwait:


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Invisiblebigfootscreepyuncl
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
    #28004926 - 10/18/22 03:32 PM (1 year, 3 months ago)

Quote:

p9hu7 said:
Final piece of equipment has arrived today, will begin work in the next day or two.







Very nice, can't wait! What kind of mechanism does the thermometer use to record the temperature so you can read if after the material has cooled down?


:popcorn:


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I :heart: 5318008


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InvisibleStipe-n CapMDiscord
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Re: Autoclave Validation and Load Penetration [Re: bigfootscreepyuncl] * 4
    #28004938 - 10/18/22 03:37 PM (1 year, 3 months ago)

It's a max registering mercury thermometer so whatever max temperature is reached the mercury will record that temperature until  the mercury is shaken back down, it won't fall to ambient temp without vigorous shaking.


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InvisiblePastywhyteMDiscord
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] * 1
    #28004979 - 10/18/22 03:57 PM (1 year, 3 months ago)

Thatโ€™s doap af


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Invisiblebakedbeings
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Re: Autoclave Validation and Load Penetration [Re: Pastywhyte]
    #28005426 - 10/18/22 07:47 PM (1 year, 3 months ago)

:popcorn:


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InvisibleStipe-n CapMDiscord
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Re: Autoclave Validation and Load Penetration [Re: bakedbeings] * 2
    #28006884 - 10/19/22 06:44 PM (1 year, 3 months ago)

https://youtube.com/shorts/JtmbI2GB8wo?feature=share

Fucking incubator is pooched right out of the box, that's what I get for ordering the $300 unit as apposed to the $900 :kingcrankey:

Nevertheless the show must go on, I am running 2 wheat jars atm with the max registering thermometer and one with an indicator strip:




Running these at the basic 10 min vent, 15psi for 90 mins.



I'll record the data and run the ampoules separately once the incubator issue is resolved.

First set of data points are in and I'm quite surprised by the results, not yet conclusive but surprising all the same.

Chemical indicator strip has changed colour indicating positive sterilization, the thermometer has registered a max core temperature of 120ยฐC:



All jars have and will receive 3 x 8oz level scoops of grain:





I have contacted the Amazon seller and they're personally testing/calibrating a new unit and sending it to me asap.

I'm now very much looking forward to having that final data point.


Edited by Stipe-n Cap (10/19/22 08:32 PM)


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