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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Cultivation Supplies, Injection Grain Bag

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OfflineSan Pedro GirlS
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Re: Autoclave Validation and Load Penetration [Re: San Pedro Girl] * 2
    #28181840 - 02/11/23 07:03 AM (11 months, 10 days ago)

Sorry, havenโ€™t had my caffeine yet. Back to autoclaves. Speaking of, does anyone have experience with the electric AAโ€™s vs the ones that require a burner?


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InvisibleAyePlusS
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Re: Autoclave Validation and Load Penetration [Re: San Pedro Girl] * 3
    #28181844 - 02/11/23 07:13 AM (11 months, 10 days ago)

Quote:

San Pedro Girl said:
Sorry, havenโ€™t had my caffeine yet. Back to autoclaves. Speaking of, does anyone have experience with the electric AAโ€™s vs the ones that require a burner?





Ya theyre wack IMO,

Cost way more

takes way longer to bring up to heat

the heating element inside the cooker means lost room to sterilize.

The timer on it doesnt go above an hr and it doesnt even control the heating element.




Have you guys seen the new nesco/carey brand electric canners? 15 psi and fits 4 quart jars (9.5qt total capacity)

Just snagged one for all my liquids/agar etc. only 115$


They even have an auto vent timer once the cooker gets to temp it starts a 10 minute timer that goes off and ypu switch it from from steaming to pressure.





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Learn about breeding

C10โ€™s agar guide
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OfflineSirPsycho
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Re: Autoclave Validation and Load Penetration [Re: San Pedro Girl] * 3
    #28181845 - 02/11/23 07:14 AM (11 months, 10 days ago)

Haven't you been paying attention, you don't need any of that. Some framer guy says IP is best for sterilize. Not sure what rough carpentry has to do with mush cult but he sounds legit


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OfflineSan Pedro GirlS
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Re: Autoclave Validation and Load Penetration [Re: SirPsycho]
    #28181871 - 02/11/23 07:49 AM (11 months, 10 days ago)



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InvisibleStipe-n CapMDiscord
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Re: Autoclave Validation and Load Penetration [Re: San Pedro Girl] * 1
    #28181887 - 02/11/23 08:06 AM (11 months, 10 days ago)

There's nothimg wromg with the 75x, many folks utilize them for bags, no good for jars.

Trashing the AA 75x only to then suggest a kitchen appliance in the same breath is pretty revealing. This fucking place is full of noobs arguing about stupidity.

Indicator strips react quite easily during a cycle, I wouldn't bother. You'd have a difficult time producing a non sterile result.

The 75x isn't for noobs running jars, not enough space, it's used for running bags. Jars are run in other units.



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Invisibleruawakeyet
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] * 1
    #28181895 - 02/11/23 08:11 AM (11 months, 10 days ago)

Aye is a noob?! That's news to me! I think that Trypt creep has got you a little worked up and maybe now you're taking it out on others?


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InvisibleMycolorado
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] * 1
    #28181901 - 02/11/23 08:13 AM (11 months, 10 days ago)

I like my 50X. It does take a sec to come to temp which is the main drawback and the timer is useless but thatโ€™s irrelevant.  Otherwise I like the set and forget aspect of it.


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Offlinerumfor69
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Re: Autoclave Validation and Load Penetration [Re: Egon_Spengler] * 2
    #28181913 - 02/11/23 08:25 AM (11 months, 10 days ago)

Quote:

Egon_Spengler said:
Quote:

TryptaminerXXXIII said:
yet P9 sits to reveal information that he shouldn't have access too.




Your account is newer than Stipeโ€™s current screen name.
If you no puppet, why you call them p9?




Fucking busted right here...can we ban this troll now?


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InvisibleStipe-n CapMDiscord
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Re: Autoclave Validation and Load Penetration [Re: ruawakeyet]
    #28181922 - 02/11/23 08:32 AM (11 months, 10 days ago)

Quote:

ruawakeyet said:
Aye is a noob?! That's news to me! I think that Trypt creep has got you a little worked up and maybe now you're taking it out on others?




I'm not on here enough to recognize every new picture and then match names. I saw a new pic, then say that 75x' are pretty much no good but check out this electric kitchen appliance.

Sounded like the same old noob voodoo I see in most threads.

I stopped posting here for a while because of it, the place has gone to shit. Posting about proper techniques and equipment turns into an all out noob shit show sooner or later.

It's more arguments and shit posting than serious cultivation, trolls and noobs forever talking shit about how they tape refridgerator boxes together with homedepot filters and then use rice cookers for broke boi tek.

Anyone who does otherwise is a gatekeeping boomer. Ridiculous.


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OfflineTryptaminerXXXIII
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
    #28181936 - 02/11/23 08:42 AM (11 months, 10 days ago)

Man I can't help but agree with that. Shit posting as in like matter a fact statements that get proven wrong? Like what we were talking about earlier?

Or TCs posting crazy stuff like

Quote:

Mycolorado said:
Nice!

You can pop bubbles with a quick hit from the torch should you so desire.



in reference to a fresh poured agar plate.


Cause that's why alot of people stop using this site. It's gone downhill hard the last decade I've been using it for sure.


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Nothing is sacred
Rest In Paradise
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InvisibleMycolorado
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Re: Autoclave Validation and Load Penetration [Re: TryptaminerXXXIII] * 1
    #28181942 - 02/11/23 08:44 AM (11 months, 10 days ago)

LOL


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Offlinerumfor69
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Re: Autoclave Validation and Load Penetration [Re: Mycolorado] * 4
    #28181944 - 02/11/23 08:45 AM (11 months, 10 days ago)

That's it...popping bubbles...this site is done for now :laugh2:


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Invisiblemind.at.large
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Re: Autoclave Validation and Load Penetration [Re: San Pedro Girl] * 4
    #28182018 - 02/11/23 09:22 AM (11 months, 10 days ago)

Quote:

San Pedro Girl said:
Sorry, havenโ€™t had my caffeine yet. Back to autoclaves. Speaking of, does anyone have experience with the electric AAโ€™s vs the ones that require a burner?



Obviously to each their own but I very much prefer the electric ones to the propane ones. Sure they are slower to heat up and you lose a small amount of space to the heating element, but not needing to babysit a cooker for 3 hours saves so much time. And if you throw a timer on it, you donโ€™t have to be there at all. You know how much stuff Iโ€™ve sterilized while I was sleeping?? Sooo much

Also, damn this thread has really turned into a shit show. I appreciate ya p9 for not only putting in the work figuring this out but for posting accurate results even if they may have contradicted your previous thoughts. Thatโ€™s true science right there.


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InvisibleAyePlusS
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] * 1
    #28182049 - 02/11/23 09:40 AM (11 months, 10 days ago)

Quote:

Stipe-n Cap said:
There's nothimg wromg with the 75x, many folks utilize them for bags, no good for jars.

Trashing the AA 75x only to then suggest a kitchen appliance in the same breath is pretty revealing. This fucking place is full of noobs arguing about stupidity.

Indicator strips react quite easily during a cycle, I wouldn't bother. You'd have a difficult time producing a non sterile result.

The 75x isn't for noobs running jars, not enough space, it's used for running bags. Jars are run in other units.






Chill out bud.. troll got you all worked up. She asked specifically about gas vs electric sterilizers so I was comparing the AA75x it to my 1941โ€™s,

Iโ€™ve run both for years at different places/ farms. I much prefer the sterilizers that i run on propane. They save me an hour or so per cycle. Iโ€™m a busy person, i run a gourmet farm that produces 100+ lbs a week in additin to the stuff i post about here.. The cost being almost double, lower capacity the high electrical requirements for the 75xโ€™s make them a no go for my operation.

This is my setup for sterilizing grain. Iโ€™m no amateur running a few jarsโ€ฆ





I do pour a shitload of plates though, i pour whats in my sig once or twice a week

and being able to run a tabletop fully enclosed unit strictly for liquids and agar (in the positive pressure lab Iโ€™m designing and building), is very appealing,

so i was just asking if anyone had seen the nesco/carey, which is actually rated for proper sterilization and runs at a higher PSI than the instapot..


--------------------
Learn about breeding

C10โ€™s agar guide
Good surface conditions = Good pinsets
Read more, post less.
๐Ÿ…‚ ๐Ÿ„ฐ ๐Ÿ„ผ ๐Ÿ„ด  ๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ
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OfflineLand TroutM
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Re: Autoclave Validation and Load Penetration [Re: mind.at.large]
    #28182057 - 02/11/23 09:43 AM (11 months, 10 days ago)

Quote:

mind.at.large said: You know how much stuff Iโ€™ve sterilized while I was sleeping?? Sooo much.



my god, this is amazing.


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InvisibleMycolorado
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] * 1
    #28183788 - 02/12/23 10:32 AM (11 months, 9 days ago)

Quote:

Stipe-n Cap said:
Are you farmer? Yeah  the mods confirmed it, how the fuck else could I possibly know.

You seem so certain, probably a puppet yourself.



:puppet:


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InvisibleStipe-n CapMDiscord
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Re: Autoclave Validation and Load Penetration [Re: Mycolorado] * 3
    #28183791 - 02/12/23 10:33 AM (11 months, 9 days ago)

Douche was clearly QM33, banned for puppetry, lol.


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OfflineDharmaForKarma
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap] * 4
    #28188603 - 02/15/23 12:38 PM (11 months, 6 days ago)

Pardon the interruptionโ€ฆhereโ€™s another data point for anyone interested in the Instant Pot.

I use an IP Duo Plus 8qt because I already had it and it works. Holds 4 quart jars upright, on the trivet. Mostly using oats, and never a green jar ever. Until now.

Based on results shared here I reduced my cook time from 2:45 to 2:00. Eight uninoculated quart jars went green pretty quickly. Stipeโ€™s testing was with a smaller IP and a single jar (iirc). My results are in line with his and not surprising. More jars need more time than a single jar.

Lesson learned. Went back to the longer timer setting and no trouble since.


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Offlinenakadash1
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Re: Autoclave Validation and Load Penetration [Re: Stipe-n Cap]
    #28190899 - 02/16/23 11:42 PM (11 months, 4 days ago)

Quote:

Stipe-n Cap said:
Makes excellent food ๐Ÿ‘Œ

The gf almost killed herself a couple of times, had to keep telling her "no you cant take the lid off to make it cool faster" lel

Maybe not kill but definitely scald to the point of wishing for death





Oh my god, how was this a recurring incident? The first time I opened a hot PC (fully depressurized) the steam coming out almost burned my forearms through my sleeve and I almost dropped the lid. Now I never mess with it until its completely cool.

**In defense of my idiocy -- I freaked out because I realized that I didn't have 3qt of water like the manual recommended so I was adding more water just in case.

Btw, the insta pot failing to sterilize a pint jar even run overnight must mean that it fails to achieve the minimum temperature right?

Before I read the comments in this thread undermining the validity of sterilization indicator strips, I used some 3m Sterigage 1432b strips and found that it fails on 1Qt jars loaded with 400g of millet with a 45min PC cycle, but I got pass results with 60 minutes (one strip buried in the middle of the grain, the other between the grain and the sidewall).

I don't have any kind of bio lab experience so I'm not even sure if these results count as pass -- some strips are dark black all the way through while others are dark on the edges with grey in the middle. I couldn't find any documentation clarifying how to interpret these results so for now I'm just PCing a bit longer.



Btw are there any physics experts out there who can explain why venting is necessary? The reasoning I've read has always been that the "atmosphere" must be released to make room for steam, but isn't steam just atmospheric gas that is saturated with water vapor? So if one were to not 'vent', wouldn't all the atmospheric gas in the PC eventually become saturated with water vapor and become steam? Or is it actually true that failure to vent means that steam cannot fully occupy the space in the chamber due to pockets of atmospheric gas?


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#1 Tek
With utmost care and precision,I work to achieve my fungal mission.
Sterilizing all my tools and space,to ensure not a single spore is out of place.
I carefully select the fungal strain and onto the agar, I make it rain.
With a steady hand and gentle touch, I inoculate the plate without a hitch.
Success is sweet when it's pure and true, and my agar plates are a testament too. A job well done, with no contamination, my fungi inoculation, a thing of  admiration.
JK NO
--Bacteriological Virtuoso aka Master of Mold


Edited by nakadash1 (02/17/23 12:04 AM)


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InvisibleStipe-n CapMDiscord
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Re: Autoclave Validation and Load Penetration [Re: nakadash1]
    #28190909 - 02/17/23 12:18 AM (11 months, 4 days ago)

Daltons law, it's at the end of the OP.

I'll read the rest tomorrow, time for bed.


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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Cultivation Supplies, Injection Grain Bag


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