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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: ruawakeyet] 4
#28058014 - 11/18/22 10:26 AM (1 year, 2 months ago) |
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I'm not sure I'd have to check but it's definitely over 1k$.
I have some plans in the future to create some cult related merch to fund my endeavors, so we'll see how it goes. Im happy to pay the bill on this one but I have some big plans for future endeavors so merch seems reasonable and not too douchey
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] 2
#28058025 - 11/18/22 10:30 AM (1 year, 2 months ago) |
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I'll be looking forward to it!!!!
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: ruawakeyet] 4
#28058428 - 11/18/22 03:29 PM (1 year, 2 months ago) |
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Results are in for the IP 90 minute cycle:
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DharmaForKarma
Tub monkey


Registered: 04/24/20
Posts: 565
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap]
#28058517 - 11/18/22 04:11 PM (1 year, 2 months ago) |
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Quote:
p9hu7 said: Results are in for the IP 90 minute cycle
We have a winner! Super cool.
Currently I run 4 quarts for 2:45. Will try 2:00 next time and a batch at 1:30. If there are any differences Iβll share with the community.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: DharmaForKarma] 2
#28058531 - 11/18/22 04:19 PM (1 year, 2 months ago) |
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Despite the possibility of getting away with a shorter cycle I would still personally recommend the 2 hour cycle with a 15 minute vent for all smaller pressure cookers. Less is not more, redundancy measures are key.
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DharmaForKarma
Tub monkey


Registered: 04/24/20
Posts: 565
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] 2
#28058546 - 11/18/22 04:25 PM (1 year, 2 months ago) |
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Quote:
p9hu7 said: Despite the possibility of getting away with a shorter cycle I would still personally recommend the 2 hour cycle with a 15 minute vent for all smaller pressure cookers. Less is not more, redundancy measures are key.
Point well taken. Cutting the time down could be a false economy. Why take a risk with an insignificant potential reward.
This whole thing is about do they or donβt they.
They do. They definitely do.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: DharmaForKarma] 2
#28058641 - 11/18/22 05:07 PM (1 year, 2 months ago) |
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Alright, the amps are in the incubator for the presto 1 hour cycle. This is the last I'll be doing of these because finding the shortest cycle possible seems unnecessary and I'm running out of amps, I'd rather run bags at this point.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] 3
#28059896 - 11/19/22 11:17 AM (1 year, 2 months ago) |
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Bags are on deck today. 6 x 3t π¦ bags in the presto, central bag will contain the BI and max registering thermometer.
IP will get 1 bag with BI.
Each bag will receive 3 quarts (4 myco quarts) of millet. Both cycles will run for 3.5 hours with a 15 min vent.
Edited by Stipe-n Cap (11/19/22 11:31 AM)
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap]
#28059898 - 11/19/22 11:18 AM (1 year, 2 months ago) |
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--------------------
I 5318008 NOT a virgin!
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 15 hours, 42 minutes
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Excited to see this
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: rumfor69]
#28060278 - 11/19/22 03:28 PM (1 year, 2 months ago) |
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So the IP is able to comfortably fit a 4lb (3 quart/4 myco quart) 3t spawn bag with a plate on top.
 
I've got the presto loaded up as well.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,901
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap]
#28060281 - 11/19/22 03:29 PM (1 year, 2 months ago) |
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--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
π
π
π
£π
£π
π
π
π
π
π
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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bakedbeings
orbiter of truth


Registered: 09/01/20
Posts: 4,218
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap]
#28060313 - 11/19/22 03:43 PM (1 year, 2 months ago) |
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Quote:
p9hu7 said: So the IP is able to comfortably fit a 4lb (3 quart/4 myco quart) 3t spawn bag with a plate on top.
not bad
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: bakedbeings] 4
#28060333 - 11/19/22 03:56 PM (1 year, 2 months ago) |
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So I didn't run a vid for the 1 hour presto cycle, I'm sure everyone gets the process at this point. The 1 hour cycle passed verification:

That's going to be the last of the short cycles, and if these 3.5 hour cycles both pass I'm likely going to just call it a day on these tests. I'd say I've been sufficiently thorough at this point.
I'll update the OP in a bit.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] 1
#28060421 - 11/19/22 05:14 PM (1 year, 2 months ago) |
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The only thing I wonder about is if the resistance value of the grain is enough to shield the heat where the ampoule is made of more conductive material and thus doesnβt mimic the heat resistance of an individual grain despite being in the relative same location as the most susceptible grains would be.
Iβm probably overthinking
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: π
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Re: Autoclave Validation and Load/Cycle Verification [Re: Pastywhyte]
#28060444 - 11/19/22 05:26 PM (1 year, 2 months ago) |
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Quote:
Pastywhyte said: The only thing I wonder about is if the resistance value of the grain is enough to shield the heat where the ampoule is made of more conductive material and thus doesnβt mimic the heat resistance of an individual grain despite being in the relative same location as the most susceptible grains would be.
Iβm probably overthinking
Or not.
p9 I know you'll think it's silly but it's worth a shot - what would you think of shielding the ampoule with bursted grains mushy doe, just the thickness of a grain like few millimeters thick
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,901
Loc: Rent free in your head
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Re: Autoclave Validation and Load/Cycle Verification [Re: Rusty2096]
#28060450 - 11/19/22 05:29 PM (1 year, 2 months ago) |
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Lmfao
"what if you build a grain around it?"
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
π
π
π
£π
£π
π
π
π
π
π
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
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Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap]
#28060456 - 11/19/22 05:33 PM (1 year, 2 months ago) |
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Yeah, pretty much that
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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bakedbeings
orbiter of truth


Registered: 09/01/20
Posts: 4,218
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Re: Autoclave Validation and Load/Cycle Verification [Re: Rusty2096] 1
#28060477 - 11/19/22 05:44 PM (1 year, 2 months ago) |
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Quote:
p9hu7 said: Yes the ampoule is the perfect device for load verification, it's designed for that exact purpose.
Think of it like this, the center of the grain jar will be the zone of maximum insulation from heat penetration; the ampoule exterior is plastic, the interior is glass filled with endospore solution. If the ampoule is sterilized when exposed to x cycle then any grain at the center or any other location of the load would also be sterile because the ampoule is insulated by the mass of grains surrounding it and the body of the amp itself. The thermal energy will have to not only penetrate the grain mass but also the ampoule which also implies that the trapped gasses have been purged to allow for penetration of the load. If endospores are destroyed in the central mass of the load they will be destroyed throughout.
The biological indicator ampoule is the benchmark verification process for medical and pharmaceutical sterility assurance, lives depend on it.
This is why I've spent this much time and money on this, it will render an indisputable result either way.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Autoclave Validation and Load/Cycle Verification [Re: Pastywhyte] 1
#28060480 - 11/19/22 05:47 PM (1 year, 2 months ago) |
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The mass of grains surrounding the ampoule shields the ampoule sufficiently to give an accurate reading. The load itself whether it be grains, scrubs, or any other material will either resist penetration to the point of failure, or it won't.
This is an integral design feature of the ampoule and why it's used to verify loads, so it stands to reason that grains aren't particularly exceptional; if the ampoule is sterilized central in the load then all other locations within the load have also been sterilized.
The center would be the most resistant to sterilization due to the material itself and wouldn't be effected by the ampoule housing. The load IS the shield.
This is why it's a load penetration test as well as cycle verification: the question is are these loads too dense to be penetrated by the cycle, and is the cycle duration sufficient to penetrate the load.
Once a load had been verified then you can change the cycle exposure to verify what is the minimum effective cycle.
So the answer is yes the load can be sufficiently penetrated by steam;
Yes the chosen cycles have been sufficient to sterilize the load.
If the body of the ampoule had a construction considerably more robust and thermally resistant than the loads it's subjeced to then the reading would render a false negative. The ampoule housing must be cunductive, this is why the endospores are suspended in solution and not in a solid mass.
Edited by Stipe-n Cap (11/19/22 06:41 PM)
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