Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | Next >
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada Flag
Trusted Cultivator
Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] * 5
    #28055087 - 11/16/22 07:42 PM (1 year, 2 months ago)

OP has been updated with the IP autoclave validation results:



Extras: Filter Print Post Top
OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,901
Loc: Rent free in your head
Last seen: 2 hours, 49 minutes
Trusted Cultivator
Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] * 2
    #28055100 - 11/16/22 07:46 PM (1 year, 2 months ago)



--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


Extras: Filter Print Post Top
OfflineRusty2096
rah rah raw in Lady gaga
I'm a teapot User Gallery


Registered: 08/23/22
Posts: 4,946
Loc: ๐ŸŒŒ
Last seen: 3 days, 21 hours
Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap]
    #28055115 - 11/16/22 07:54 PM (1 year, 2 months ago)

p9, I just watched the vid. I couldn't help but notice your left thumb is scarily flexible :eek:


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada Flag
Trusted Cultivator
Re: Autoclave Validation and Load/Cycle Verification [Re: SirPsycho] * 3
    #28055118 - 11/16/22 07:56 PM (1 year, 2 months ago)

I love unexpected results, you know the science is good when your results contradict your expected outcome.

:havesomescience:

Quote:

Rusty2096 said:
p9, I just watched the vid. I couldn't help but notice your left thumb is scarily flexible :eek:




Nothing gets by you folks :lol:


Extras: Filter Print Post Top
OfflineSo Anyway
Boner donor


Registered: 07/11/21
Posts: 543
Loc: On the map
Last seen: 1 year, 14 days
Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] * 2
    #28055130 - 11/16/22 08:03 PM (1 year, 2 months ago)

Very interesting results with some serious implications for people who already own this sort of hardware and who want to dip into grains. I've been running some small grows in a ninja foodi the past couple months and while the amount of spawn that can fit is limited and I'm not sure the yields for me have been much more pronounced than running a similar amount of pf tek jars, it has been a fun experiment to see just how far I can push the device.

Many thanks for putting in the time, money and effort into this experiment that helps put some definitive science behind all the ways we as a community are sterilizing grain!


--------------------
So anyway, uh...

Photo journal


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada Flag
Trusted Cultivator
Re: Autoclave Validation and Load/Cycle Verification [Re: So Anyway] * 2
    #28055139 - 11/16/22 08:09 PM (1 year, 2 months ago)

With the amount of interest in these units it needed to be done. I cannot guarantee that other models of IP are as effective, nor does it cover other brands.

I was previously under the impression that  instant pots failed validation (could have been a different model) so I'm glad that I purchased one to test myself.

Glad to contribute to the wealth of knowledge already found here, I'm sure this info will be useful.


Extras: Filter Print Post Top
Invisibleruawakeyet
 User Gallery

Registered: 05/03/21
Posts: 1,862
Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] * 1
    #28055176 - 11/16/22 08:36 PM (1 year, 2 months ago)

I wonder if past tests were conducted using the sterilize function? I'm not at all familiar with IPs, but caught that in the video.


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada Flag
Trusted Cultivator
Re: Autoclave Validation and Load/Cycle Verification [Re: ruawakeyet] * 1
    #28055319 - 11/16/22 09:38 PM (1 year, 2 months ago)

I didn't use the sterilize function due to the short timer so I used the pressure cook mode instead.


Extras: Filter Print Post Top
Invisiblebakedbeings
orbiter of truth
 User Gallery

Registered: 09/01/20
Posts: 4,218
Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] * 2
    #28055331 - 11/16/22 09:44 PM (1 year, 2 months ago)

i think he means maybe it has failed validation with the 1 hr sterilization setting


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada
Trusted Cultivator
Re: Autoclave Validation and Load/Cycle Verification [Re: bakedbeings] * 2
    #28055361 - 11/16/22 09:56 PM (1 year, 2 months ago)

Oh, rgr.

The only previous test that I'm aware of was 4 hours with BI in a pint jar. I asked  the person about it and I believe they failed to vent, whoops.


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada
Trusted Cultivator
Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] * 3
    #28055406 - 11/16/22 10:31 PM (1 year, 2 months ago)

Oats have been added to the OP which completes cycle verification at 90 minutes  and load verification for each grain.

I don't forsee other untested grains being any different. Millet should cover all seeds, wheat is very similar to rye, and corn is a large as any other ridiculously large grain:lol:



Extras: Filter Print Post Top
Invisibleruawakeyet
 User Gallery

Registered: 05/03/21
Posts: 1,862
Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] * 1
    #28055561 - 11/17/22 12:52 AM (1 year, 2 months ago)

I just hope you have some of those vials left to test minimum times! Are you still planning those tests when you have time?


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada
Trusted Cultivator
Re: Autoclave Validation and Load/Cycle Verification [Re: ruawakeyet] * 5
    #28055722 - 11/17/22 07:02 AM (1 year, 2 months ago)

I should have enough, I'll likely order another box soon anyways.

Omw out to grab some pint jars so that the load in the IP is representative of actual practice.

I'll run the presto as well to test 60 minute cycle.


Edited by Stipe-n Cap (11/17/22 08:27 AM)


Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/13/02
Posts: 9,284
Loc: South Africa
Last seen: 2 hours, 4 minutes
Trusted Cultivator
Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] * 2
    #28055864 - 11/17/22 09:10 AM (1 year, 2 months ago)

That's going to be interesting because I ran 500ml jars for years with a 1hr pc.


Extras: Filter Print Post Top
Offlinehazyhorse
scoobin
I'm a teapot Unread Journal User Gallery


Registered: 03/19/19
Posts: 3,820
Last seen: 9 hours, 41 minutes
Re: Autoclave Validation and Load/Cycle Verification [Re: DERRAYLD] * 3
    #28055915 - 11/17/22 10:16 AM (1 year, 2 months ago)

this is so bad ass, man. seriously youโ€™re doing the lords work out here with these tests. really fucking sick to have some hard data on all this. super shocked at the IP results but honestly so stoked. if it opens doors for people to cultivate with what they have thatโ€™s awesome. if thereโ€™s ever any sort of fund to reimburse you for all this i would gladly contribute

on venting, thatโ€™s something iโ€™ve recently been made aware of. i know the info has been out there, but when you read โ€œvent for 15 minutesโ€ when youโ€™re first learning to use your PC you kinda just roll with it. been doing longer vent times on grains recently & very curious to see how it changes things for me

thank you again p9. really amazing work


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada
Trusted Cultivator
Re: Autoclave Validation and Load/Cycle Verification [Re: hazyhorse] * 4
    #28056008 - 11/17/22 11:26 AM (1 year, 2 months ago)

Thanks man.

So I have a pint jar running in the IP as we speak, 90 min cycle with 10 min vent.:popcorn:

Even if the 3 hour cycle ia whats required you can get 4 pint jars sterilized with almost zero effort. Flash prepped millet is a great combo. my opinion has gone 180 on this now that I know that it's actually capable as an autoclave it's a pretty convenient machine for low volume.

4 pint jars are all you need to inoculate 4 x 5lb supplemented hwfp blocks, so potentially a great tool for low volume gourmet.


Extras: Filter Print Post Top
Invisiblebigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap] * 1
    #28056017 - 11/17/22 11:31 AM (1 year, 2 months ago)

I'm excited to see how the pint jars do in the Presto for 60 minutes. I want to run pints on occasion so I can fit more jars in the PC at a time and run a lot of different varieties for clone hunting but I don't want to run full 6qt shoeboxes.

Thanks again p9, you da man!


--------------------



I :heart: 5318008


NOT a virgin!


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada
Trusted Cultivator
Re: Autoclave Validation and Load/Cycle Verification [Re: bigfootscreepyuncl] * 1
    #28056033 - 11/17/22 11:45 AM (1 year, 2 months ago)

I won't be running pints in the presto, just the IP. Presto gets 1L jars for 60.

Coming up next.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,268
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Autoclave Validation and Load/Cycle Verification [Re: Stipe-n Cap]
    #28056034 - 11/17/22 11:48 AM (1 year, 2 months ago)

:popcorn:


Extras: Filter Print Post Top
InvisibleZifozonke
Stranger
 User Gallery

Registered: 03/24/19
Posts: 1,258
Re: Autoclave Validation and Load/Cycle Verification [Re: mushboy]
    #28056094 - 11/17/22 12:41 PM (1 year, 2 months ago)

Awesome stuff :rockon:


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | Next >

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* 1.5 liter Bags (free standing -impulse seal- zip lock - autoclave - w/micron filter)
( 1 2 all )
agar 6,261 36 03/27/06 10:44 AM
by
* Another Autoclave Question Researcher 771 4 11/09/05 03:49 PM
by backupwards
* Autoclave Q ***UPDATED w/ additional question. Anything4Poppies 857 10 10/22/05 02:55 PM
by backupwards
* autoclave help please heathadelic 1,346 8 08/14/01 02:50 AM
by heathadelic
* using an autoclave bag buddhasninja 779 1 12/22/06 02:53 PM
by soulman1990
* Minimum substrate depth. Sporetastic 2,810 5 05/18/07 07:34 AM
by atomic1
* autoclave users closetgrower 1,120 13 05/25/05 09:57 PM
by Blue Helix
* Cheap autoclave chills420 1,137 6 02/05/03 09:28 AM
by tripndicular

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
7,259 topic views. 37 members, 228 guests and 46 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.039 seconds spending 0.014 seconds on 15 queries.