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InvisibleCultiV8
cogito, ergo rum
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Registered: 08/16/14
Posts: 305
Re: Cultivation General Discussion [Re: hazyhorse]
    #27964604 - 09/23/22 04:12 PM (1 year, 5 months ago)

Can anything be done to limit fermentation & bacterial growth while grain is sitting waiting to go in the PC? Would the centers of jars/bags get cool enough quick enough to inhibit this type of activity if they were loaded into the fridge warm?

Quote:

tedoro said:




All bags: Birdseed, soaked 10 hours in what started as 140f water.

Bags on the right: Loaded immediately into PC and ran.

Bags on the left: Sat in loaded bags, wet, for 5 hours waiting their turn to get PC'd



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OfflineNichrome
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Re: Cultivation General Discussion [Re: CultiV8] * 3
    #27964629 - 09/23/22 04:25 PM (1 year, 5 months ago)

Refrigerator.


--------------------
Once you've got something figured out completely, you're stupid.

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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: Nichrome] * 4
    #27964899 - 09/23/22 06:55 PM (1 year, 5 months ago)

FIMETARIA POST
its 24.09 of september (repeat)

and ive seen activity again the last days

fimetarias are popping up (2-3 small fruits 6h ago)
after the day/night temps hit sub 15 and 2-3 days of raining
which again coincides towards aututmn fruiter
understanding of fimetaria
and its general similarily with semilanceata

then again in my country there's semis fruiting sometimes in august
and the question of whether or not they TRULY
share habitat

recently also ran across a guy who claimed fimetarias only
fruited from manure

there's a race going on to find the first fimetaria in my country now, get a ITS sequence ID since its a national first

tonight at a drug reform party i met one of the 2 persons who possible sent me the fimetaria printed as semilanceata, and he swore a holy oath to search for it every season the rest of his life

back in the day, 7 years ago, anglerfish a cool guy here
at the forums, made a post about
finding fimetaria in norway the first time, ID by microscopy

but learning from my own semi/fimetaria find/scandal,
microscopy is not hard science. its soft evidence.

only ITS sequences is hard evidence, so anglerfish
doesnt constitute a find applicable as hard evidence
and documentable to the state,

im this year gathering the pictures of what people post as semilancetas... watching ID threads.

im seeing the brown "bruising" or pigment on some stems that first time pickers post of fruits posted as semilanceatas, to me looking like also fimetara-possibles

ive yet to see a norwegian wild fimetaria annulus in those threads tho


not that fimetarias dont actually exist in my country, i mean, they are growing in my garden so fuck you anyone who thinks they cant make it here.. im 200% sure we'll see them there next  year too

I grow psilocybe dang butt


--------------------
Willpower is the one true virtue


Edited by smalltalk_canceled (09/23/22 06:58 PM)

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Offlinenektar61S
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Re: Cultivation General Discussion [Re: mind.at.large]
    #27965300 - 09/23/22 11:37 PM (1 year, 5 months ago)

Quote:

mind.at.large said:
Quote:

nektar61 said:
The other enzyme turns psilocin into a non-psychedelic blue indole chain that is related to natural Indigo dye.



Do you know if anyone has used psilocybes to dye? I’ve done it with many other mushrooms but that blue would be super cool!





I don't know much about dying, but I think it would work if you had some way to "fix" the dye.

I do know that if you make an everclear alcohol / water tincture of shrooms, it's fucking BEAUTIFUL royal blue at first. Next day it's mud brown.

Would be fun to make that drink, pass it around a party in a clear pimp cup while it's still blue.

Next day when brown it's lost a good bit of its potency too.

Psilocybin turns to psilocin to be active, but psilocin isn't very stable. Shrooms have both, and in different ratios from type to type and clone to clone.

Look at the 2021 Psilocybin Cup results.


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Offlinenektar61S
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Re: Cultivation General Discussion [Re: hazyhorse]
    #27965309 - 09/23/22 11:46 PM (1 year, 5 months ago)

Quote:

hazyhorse said:
honestly man don’t get too wrapped up in calculating every milligram, you can literally just eyeball it & it’ll be fine. anything between 1:1-1:4 spawn to sub is gonna work great. i usually do around 1:2-1:3 eyeballed with a 1/2” top layer of coir at the end. if your sub is about 3” deep you’re fine




This.

I've never measured.

Coir I first throw in a big insulated water thing, like what outdoor workers use. I pour boiling water on it, stir it up, let it sit until room temp.

I toss a (myco) quart of spawn in a 16 quart tub I use for mixing. I add some filtered well water, some verm, and some coir. If coir is still warm, I pour the water on the coir to get to room temp.

Mix it up with ladle until when squeezing in a clean or gloved hand, some drops come out. Not none, not one, not a steady stream, just like 10 or 15 drops or so.

Then I take a 7 quart shoebox, put in a liner (trash bag), ladle the mix in.

Don't pack it, just put it in, make sure it gets in the corners. Fill it to an inch or two from top. Sprinkle on a quarter inch more dry verm as psuedo casing. Trim the bag to just above the surface.

Stick it on a shelf in the shroom room, mist the top layer, put the lid on upside down, just sitting there, not tight.

Give it 6500 k light about half of each day. Fan with the lid once or twice a day. Maybe doesn't need that but I do it. It's fun.

Mist it a few times a week. More in the summer. Less in the winter.

Keep the room 70 f with a little heater and fan blowing away from the shrooms. That turns off in the summer, I have a humidifier. 

In about 12 days I get pins.

(double post but two long answers to two different questions)


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OfflineGrind365
AlwaysGrinding

Registered: 08/07/22
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Last seen: 1 year, 1 month
Re: Cultivation General Discussion [Re: Grind365]
    #27965356 - 09/24/22 01:02 AM (1 year, 5 months ago)

Quote:

Grind365 said:
Is this mold that hasn't sporulated yet?




It's definitely mold. Hasn't sporulated yet, but it's spread enough that I definitely recognize it for what it is. :sad:


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InvisibleAphexPin
Strange

Registered: 02/22/22
Posts: 464
Re: Cultivation General Discussion [Re: Grind365]
    #27966127 - 09/24/22 02:19 PM (1 year, 5 months ago)

Having a major issue with my unicorn bags where the bottom factory sealed gusset leaks (on the interior of the fold, so I think it's unlikely it's my PC or something poking holes in it). I use a mesh pizza tray as a false bottom in 75x and 25x. Should I contact unicorn? It's pretty ridiculous, but I want to rule out myself first.

Edited by AphexPin (09/24/22 02:19 PM)

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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: AphexPin] * 1
    #27966135 - 09/24/22 02:25 PM (1 year, 5 months ago)

Unicorn bags have defaults, this is pretty common, apparently.

If you're having an issue with a seal then I'd recommend using your own impulse sealer (if you have one) to back up any faulty seem.

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Invisiblesandman420
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27966136 - 09/24/22 02:26 PM (1 year, 5 months ago)

Sounds like a bad batch where the machine setting got messed up. Im sure they will replace. Or just hit it with your own sealer like the most excellent p9 said.


--------------------
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Edited by sandman420 (09/24/22 02:27 PM)

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InvisibleAphexPin
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Posts: 464
Re: Cultivation General Discussion [Re: sandman420] * 1
    #27966157 - 09/24/22 02:41 PM (1 year, 5 months ago)

Well I just sent 'em an email, it's hard to say how many are bad. The first few bundles were fine but this has been going on way too long, took me awhile to be confident it was the bags themselves. I have some of a different size and thickness and those have zero problems. But yeah to use up the remainder I'll start sealing the bottoms before I even PC them, good idea and thanks. Hoping this fixes up my lingering contam issue.

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OfflineGrind365
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Re: Cultivation General Discussion [Re: AphexPin]
    #27966227 - 09/24/22 03:30 PM (1 year, 5 months ago)

So I am going to try a method of prep that I haven't really used before and I figured I'd consult here first since some of you guys have probably tried it before.

I'm running WBS.

I normally soak, boil, drain, steam dry, load and PC.

I want to try instead.
12-24h cold soak. Drain off all water. Load semi damp and PC.

I'm assuming most if not all excess moisture will be absorbed during PC cycle and remaining condensation should clear up within a day or two.

Does this sound like it'll work or do I need to dry grains before loading and PCing?

Thanks in advance for any and all help.


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OfflineNichrome
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Registered: 12/17/18
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Re: Cultivation General Discussion [Re: Grind365] * 2
    #27966260 - 09/24/22 03:53 PM (1 year, 5 months ago)

That should work very well Grind, and is my preferred method for a lot of wbs mixes and types of millet and other seeds.


--------------------
Once you've got something figured out completely, you're stupid.

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Offlinewild_aussie
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Registered: 01/05/22
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Re: Cultivation General Discussion [Re: Smellyhobbit] * 3
    #27966397 - 09/24/22 05:36 PM (1 year, 5 months ago)

Quote:

Smellyhobbit said:
A quart scoop sounds great. I have a big spoon that fits perfectly into a wide mouth quart jar, so loading it with coir takes maybe 10 seconds. But if I could just have a scoop that would be awesome.



Wally world sells Kool aid pitchers that are a quart.  I used the top half of a plastic 2qt pitcher for my SAB holes and cut the bottom at the quart mark vola qt scoop.
@wildcat1883 fyi bruising in no way show th potency of the shroom.  Guerilla had a whole tub of tidal waves lots of blue and bunk
Pic tax my culture hunting jar for pulling pins


--------------------
BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
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OfflineGrind365
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Registered: 08/07/22
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Re: Cultivation General Discussion [Re: Nichrome]
    #27966531 - 09/24/22 07:35 PM (1 year, 5 months ago)

Quote:

Nichrome said:
That should work very well Grind, and is my preferred method for a lot of wbs mixes and types of millet and other seeds.



Cool sounds good thanks for the reply Nichrome.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Grind365] * 2
    #27967135 - 09/25/22 08:32 AM (1 year, 5 months ago)

Quote:

Farm3r said:
Quote:

Rotnpins said:
Quote:

Farm3r said:

Who said dunk spawn lol?





https://www.shroomery.org/forums/showflat.php/Number/17616982/vc/1




Lol that's a decade old. And really was probably the introduction of the community realizing how much water is lost during colonization. Also not a horrible idea, although it could be directly reaccounted for by adding that much water it would have absorbed into the tub. I feel like that's actually a crucial factor of us understanding how subs and their moisture content also act inside tubs of different conditions.
It's not like gourmets where the teks have been tried for decades around the globe. This community has been finding the best way to grow cubes for decades.
If your hating on Frank, gtfo, he's done more here than most ever will.

And whereas that is completely besides the point, and idk why you brought that up, it still is not even comparable to the scientific evidence that backs a Instapot efficiency.

This isn't an argument. This is a discussion.




Dunking myceliated spawn is 100% pointless. Saying "Frank did x so it must be true" is an argument to authority rather than using critical thought.

Also, in terms of an instapot functioning as an autoclave:

Autoclaves have very specific design features that allow them to be effective against viruses, diseases, and most notably in our case: thermophilic bacterial endospores.

A 23 quart presto can be used as an autoclave because it shares many of those design features. Autoclaves are capable of :

Maintaining even an even working temperature across all surfaces, this is critical due to the way surfaces interact with internal gases, cold spots will cause an autoclave to fail verification due to the insulative properties of cool trapped gases; this is tested via Bowie-Dick test;

An instant pot is not capable of this.

Purge cycle:

Autoclaves and prestos require purging of trapped atmospheric gases in order to reach an appropriate internal temperature, if this is not completed the pressure guage will give a faulty temperature reading which will mean that your load will not sterilize completely;(major reason why instapots fail at cult work)

Instant pots are not ccapable of this.

Autoclaves can sustain operating temperature and pressure for the appropriate time frame: minimum 15psi for a MIN 90 mins. The R value of grains is quite high, load penwtration requires long exposures.  More is more, less is less.

Instant pot....

There's nothing eletist about knowing how shit works.

Liquid media is liquid, water is highly conductive and instruments are considered a very light load. You can get away with agar and LC broth, but still, don't.

Maybe you can run liquids  in an instant pot, producing clean spawn on the otherhand....

Just because x member ran grain jars in something inappropriate for the application does not mean anything.
Good advice is good advice, the science doesn't lie.

Arguments to authority are not arguments, x member was not a TC when he ran grains in an instant pot and something tells me his spawn wasn't awesome when he did.

Doubtful he would recommend it but hey, who know's these days.

Edit:

Another mod on the shroomery discord server has tested the instant pot with biological indicator in a pint grain jar ran for 4 hours. She works in an AIDS lab.

Guess what...

It failed to sterilize.

Edited by Stipe-n Cap (09/25/22 09:04 AM)

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OfflineFarm3r
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27967195 - 09/25/22 09:03 AM (1 year, 5 months ago)

Lol you brought this over here. That wasn't even what we were talking about.

You said instapots had absolutely no idea in mycology. And I pointed out people have used them with efficiency, simple as that.




https://www.shroomery.org/forums/showflat.php/Number/27827195/fpart/173/vc/1





:picdidnthappen:


And whose that?

Also there are different models, as we've already pointed out in our other discussion.



Why did you repost this topic?


And why couldn't a instapot purge steam like a presto?

Your bringing up new points I guess. But present some proof huh?










Advice to everyone, TLDR , just go reread our convos in the quick question quick answer thread.

Edited by Farm3r (09/25/22 09:11 AM)

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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Farm3r]
    #27967201 - 09/25/22 09:05 AM (1 year, 5 months ago)

It is a topic of discussion, moved to the discussion thread.

You're combative in the face of overwhelming evidence.

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OfflineFarm3r
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27967208 - 09/25/22 09:08 AM (1 year, 5 months ago)

Of what?!

I could say that about you!


I'm done arguing with you about nothing. Sterilization is simple.


Who told you they ran bio strips in one? I want to go ask them.



Lol, tell me why a instapot couldnt sterilize when it fills itself with steam under 15psi?

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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Farm3r]
    #27967213 - 09/25/22 09:13 AM (1 year, 5 months ago)

I guess you cant read very well, I described it in great detail.

Instant pots can sterilize liquid media, is not suitable for grains due to load density and bioburden.

Why? Because it doesn't actually function like an autoclave.

They have been tested, instant pots are good at sterilizing Geobacillus stearothermophilus spores in liquid media, 4 hours in pint jars renders no effect on the biological ampule indicators used in an actual lab.

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Offlinecozmyc
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Re: Cultivation General Discussion [Re: Farm3r]
    #27967216 - 09/25/22 09:14 AM (1 year, 5 months ago)

It's pretty clear. He said previously, if you have one already, use it. Just don't buy one. Are you suggesting buying a an insta pot over a presto or autoclave?


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and that's valuable :sunny:

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