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Muad.Dweeb
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The Weirding Way - Advanced Bene Gesserit Techniques 11
#27958711 - 09/20/22 09:06 AM (1 year, 4 months ago) |
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ʬʬ ₸he Ѿeirding Ѡay ʬʬ
ϾϿ
Advanced Tools and Techniques of the Bene Gesserit for Manipulations in the Breeding Programme

[Weird- from Old Norse wyrd] The Weirding is a seemingly mystical power of select groups of people in Frank Herbert's “Dune.” Dependent upon highly developed mental and physical discipline, and sometimes the spice melange, the abilities of the Bene Gesserit- and those they train- allow them to stealthily control and manipulate individuals as they need, without detection, to orchestrate their grand breeding programme, to bring about the Kwisatz Haderach. To the Bene Gesserit, these abilities are described as Prana Bindhu control, and range from teleportation to transmutation. This section of Breed Like the Bene Gesserit contains things that, at first glance, may sound and look weird or magical. I assure you, they are not. With exceptional discipline and rigorous application, you too can learn how to levitate spores, control conception, transmute cultures, and reduce them to their base components. While attempting these procedures, you will fail. If this is because of constant contamination, you are not ready for these procedures, and need to return to developing basic cultivation skills. If not, take some Sufi advice and rejoice that you get to experience the freshness of the nubile state once more. Where possible, I will try to offer theories of the mechanisms, both personal and with links to published studies, as well as my personal notes of my own learning curve with these methods. Some will be rather involved, and I understand someone (many people) not wishing to get so involved. That is okay. Breeding isn't for everyone, and this section isn't for all breeders. It's fine to throw spaghetti at the wall, just to see what sticks- and believe when I say that you can find strange and wonderful things that way- but for the select, spaghetti art isn't enough. For the select, we judge our success by a self-directed vision, and how closely we can make reality match that vision. This is our secular magic, our Godless religion. This is our Art. Fortuna Juvat Perseverantiam (Fortune Favors the Persistent)Teks and Explainations:* Spice Agony, 1: Symmetrical Dedikaryotization* Fremen Tools, 1 - Induction Sterilizers* Trial by Gom Jabbar, 1: Trich Farming* How to Swap Mitochondria
Edited by Muad.Dweeb (01/30/23 01:13 PM)
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Muad.Dweeb
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: Muad.Dweeb]
#27958714 - 09/20/22 09:07 AM (1 year, 4 months ago) |
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Symmetrical Recovery of Neohaplonts from Dikaryon Fragmentation
Sources: A Patent
PDF below Abstract
A more recently published use of this technique
My Notes
A combination of previously outlined procedures and tools will be utilized. The primary benefits of this approach are recovery of both monokaryon types, with low mutation and damage potential. It can be thought of in two phases: the initial phase, in which the dikaryotic culture is minced, and the recovery phase, in which colonies are regenerated from the minced culture.
Quote:
Materials:
Dedikaryotizing Broth (“DB”); 2% Dextrose, 2% Peptone, sterilized 15 min. Osterizer Water agar Nutritional agar Inoculation loop Syringes or pipettes 15mL Centrifuge tubes with 9mL of sterile water, each
Set up:
1. First, assemble the Osterizer jar. If using a Waring blender, sterilize all parts that will contact the liquid and culture. With the Oster blender, I recommend grinding or filing down the blades so they are thinner, and using a polishing stone to smooth the blade edge before sterilizing. It's not necessary, but at least polishing the blade edge will reduce cell damage to the mycelium.*
* [ETA] ...actually, a fresh blade has proven ideal. A polishing stone to smooth out knicks is fine, but the best results have come from the newest, un-modified blade base I have.
2. Prepare the dedikaryotizing suspension broth in the Osterizer jar with 2% dextrose and 2% peptone. Sterilize 10-15 minutes. With the half pint jars, *100mL* is a good amount.
3. Generate clean liquid culture from agar. I recommend using the EzLC method, but any traditional method will suffice. The starting material should be dikaryotic. If blending agar cultures, they should either be soft agar (1.5%-1.75%) to ease blending, or stiff agar (2.5%-3%) to make the culture easy to scrape off the surface.
Procedure:1. Add a square of colonized agar approximately 30mm (or 10mL of LC) to the Osterizer. 2. Blend for 15-20 seconds, pulsed in 5 second increments (to reduce heating the culture). An emulsion will form, which will help to cushion and partition the cleaved hyphal compartments. The dextrose will slowly settle back into solution after a short while. 3. Incubate in situ 3-4 days at room temperature. Gently swirl the culture periodically, to prevent clumping. If new growth hasn't formed within this time frame, start over with a lower blending time, sharper blades, or both. 4. Blend for 5 seconds. This is only to fragment new growth, not completely macerate the culture. 5. After the emulsion settles, swirl well and add 1mL to a centrifuge tube with 9mL sterile water.  6. Agitate thoroughly for 10 minutes and streak on water agar or low nutrient agar (<1%). Alternatively, 1mL can be drawn up and a single drop can be added to several centrifuge tubes with ~9mL of sterile nutrient broth or water. The colonies in these tubes will grow into spheres of monokaryotic mycelium, which can be fished out and minced/streaked separately.  7. Incubate at room temperature until visible colonies form (~1mm). With an ultra fine marker, circle potential isolated colonies with at least a centimeter of isolation, and inspect the colonies under a microscope. Check each colony for absence of clamps, and absence of minute proximal colonies not casually visible. Young, isolated colonies should be considerably circular, with frequent branching of hyphae in a “peg” style. Note on the plate where there are any nearby colonies to avoid, and transfer them methodically, with great care to absolutely minimize sample size. [clamps highlighted with red]
   8. Transfer colonies into isolation on nutrient agar and label them in series (1, 2, 3, etc...) When they have grown enough to transfer from, set up a series of comparison plates with 1x2, 1x3, 1x4, etc...  9. The first recovered neohaplont will arbitrarily be labeled nuclear type A. Colonies that fuse with “1” and do not produce clamps are nuclear type A, while colonies that do form clamps after growing together are nuclear type B. For further personal confirmation, fruit the A/B cultures on a fruiting medium and observe the original fruit phenotype.    10. Label the corresponding nuclear type to the cultures, and save redundant axenic samples of both nuclear types on slants. Notes:This is a “One Pot Recipe,” if you will, designed to minimize the potential for contamination and maximize dedikaryotization. The most accessible materials possible were used to execute this procedure with repeated success (with P. cubensis) with high degree (approaching 100%) of dedikaryotization. Minor fine tuning as needed per specific strain is to be expected. Entirely different tolerances may be required for other species, but this technique should prove a good starting reference. Many published research papers using this or a similar technique call specifically for peptone type P “Oxoid” and glucose. However, standard bacteriological peptone from Hi-Media was used, and the very available dextrose is the d-glucose enantiomer. Potentially, 2% nutrient yeast could be substituted for peptone, as well. Additionally, great results have been obtained in many cases using only water in place of any nutrient broth, though colony growth is minimal. The key points to perfect are blending time, and a proper dilution after the second blending. This is a complex procedure. Always double check the work and the tek while working. Several attempts may be required for success.
Edited by Muad.Dweeb (10/04/22 11:47 AM)
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Rotnpins
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: Muad.Dweeb]
#27958730 - 09/20/22 09:23 AM (1 year, 4 months ago) |
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I've read through your other thread a couple times, good stuff thanks for sharing
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DERRAYLD
Constructus


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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: Rotnpins]
#27958736 - 09/20/22 09:27 AM (1 year, 4 months ago) |
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Muad.Dweeb
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: DERRAYLD]
#27958755 - 09/20/22 09:51 AM (1 year, 4 months ago) |
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"approaching 100%" I should qualify...
Read my notes (linked), and the "recovery rate" was 12/23, or 52.17%, which is in the range of other published results. Of those 12, identification as a mon was possible before pairing 100% of the time.
It's always a good idea to keep any old plates around while doing this for a few weeks at least. However, with the results I have been obtaining, the timeline and response of paired mycelium is pretty straight forward. When simultaneously, some plates start forming clamps, while their controls and other plates do not, you can confidently fill in that sudoku box.
-------------------- A beginning is the time for taking the most delicate care that the balances are correct. This every sister of the Bene Gesserit knows.
How to Breed like the Bene Gesserit The Weirding Way - Advanced Bene Gesserit Techniques
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Muad.Dweeb
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: Muad.Dweeb] 4
#27959002 - 09/20/22 01:40 PM (1 year, 4 months ago) |
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Induction SterilizersA couple years ago, I did a write up in the Facebook Shroomery group on Induction Sterilizers. I wasn't exactly the first person to come up with the idea, but after I made the post, a bunch of people were suddenly making the cheap, plug-and-play version I offered to people who lack solder skills and just want one as cheap as possible. And you wouldn't believe the outrageous prices people are charging/paying for a handful of chinese parts, a box and a foot pedal. So here is how you make your own. You'll Need:A ZVS Module: https://www.amazon.com/s?k=ZVS+module&crid=B9UO1MP6O3D6&sprefix=zvs+module%2Caps%2C223&ref=nb_sb_noss_1A Beefy Power Supply (12v to 24v, 10 to 20 amps) I'm partial to the Alitove brand (not affiliated): https://www.amazon.com/s?k=12v+power+supply+alitove+10A+20A&crid=3IL88KFC5CP06&sprefix=12v+power+supply+alitove+10a+20a%2Caps%2C167&ref=nb_sb_nossA Beefy Foot Pedal like this: https://www.amazon.com/Twidec-Momentary-Nonslip-Handsfree-Industrial/dp/B07XXWN331/ref=sr_1_17_sspa?keywords=foot+switches&qid=1663696911&s=industrial&sr=1-17-spons&psc=1Some connector crimps (optional, if you're adding extra wires): (you can get these from any hardware store really...) https://www.amazon.com/Connectors-Electrical-Connector-Insulated-Terminals/dp/B09FG49BZX/ref=sr_1_27?crid=24X3TXTF3DCTC&keywords=crimp+connectors&qid=1663697047&s=industrial&sprefix=crimp+%2Cindustrial%2C188&sr=1-27Optionally, you can put this in a project box to tie it all together neatly: https://www.amazon.com/s?k=project+box&i=industrial&crid=CA5XDGWX8VDJ&sprefix=project+box%2Cindustrial%2C190&ref=nb_sb_noss_1*** For the ZVS module, the 5-12v is plenty and you'll be able to sterilize your scalpels in 5-10 seconds without generating a whole lot of extra heat. Bonus, they always come with a pre-made coil, so that's one less thing to worry about. If you want the instant sterilizing of the 1000W ZVS modules, make sure you get one that comes with the coil, as a lot of them don't. Without the coil, you'll also have to get some 12g or 14g magnet wire, or thin copper tubing to make your coil.  If you can solder, soldering the coil directly to the large contact pads is preferable to the plastic screw-down terminals installed for the coil. Over time and with extended use, the terminals themselves will warp and fail. The screw-down terminals for the power supply are perfectly fine to use, though, as they do not conduct much heat.  For the power supply, the minimum/maximum requirements will usually be printed on the module PCB, or even in the description of the listing. The bare minimum you'll need is 12v that can deliver 10 amps (12v 10a), and that will drive the 5-12v ZVS just fine. The maximum for the 5-12v ZVS is 20 amps, but at 12v, that will probably burn out pretty fast. I would cap it at 15 amps for the small guy. For the 1000W module, 12v 10a is going to be the lower end of effective power, and up to 24v 30a could be used, but again it's likely to burn out sooner if you are pushing the higher values.  I like the Alitove (A Lite of Love) power supplies, personally, and they come with a screw-down adapter that makes this whole thing very seemless without buying extra parts. You need two small pieces of wire that connect + to + and – to –, then the power supply just plugs in. To add the button or foot switch, we just put it place of the wire between the + of the connector tie downs. Now, power only comes in when the switch is closed. *DO NOT POWER UP THE MODULE WITHOUT THE COIL ATTACHED* That is an excellent way to burn up the mosfets.  If you want to add a project box, some additional layout planning and drilling/cutting is required, maybe some epoxy gluing too. But that's really all there is to it.  So let's add all that up. Under 10 for a project box 10-40 dollars for the ZVS module 20-40 dollars for the power supply 5-6 bucks for crimps (maybe less) 5-10 clams for a switch That's 50-106 USD at the time of writing this in parts to have something that sterilizes blades on the low end, all the way to melting them on the high end. There are people out there using the 50 dollar option to sell for 150+. Don't be a sucker. Now if you're paying someone for one purely for the convenience or their craftsmanship, then fine, pay what you feel is fair. But know what you're getting. And how much more personal making one yourself can be.    [custom inductor skulls I have made. Do not message, I don't sell these.]
-------------------- A beginning is the time for taking the most delicate care that the balances are correct. This every sister of the Bene Gesserit knows.
How to Breed like the Bene Gesserit The Weirding Way - Advanced Bene Gesserit Techniques
Edited by Muad.Dweeb (09/20/22 01:48 PM)
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smalltalk_canceled
Babnik



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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: Muad.Dweeb]
#27959115 - 09/20/22 03:13 PM (1 year, 4 months ago) |
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-------------------- Willpower is the one true virtue
  
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The Blind Ass
Bodhi


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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: Muad.Dweeb]
#27959416 - 09/20/22 06:17 PM (1 year, 4 months ago) |
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Some things are so good they hurt because we may lack the proficiency to express our feelings regarding them. Almost like the life of a chair dog but not quite as horrific and minus all the good.
Anyways. This thread is one of those things, but with all the good and no chair dog... 
-------------------- Give me Liberty caps -or- give me Death caps
Edited by The Blind Ass (09/20/22 06:26 PM)
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DERRAYLD
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: The Blind Ass]
#27959970 - 09/21/22 12:19 AM (1 year, 4 months ago) |
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Damn those skull induction coils are ridiculous dude,
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hummingbird

Registered: 06/29/14
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: DERRAYLD]
#27960044 - 09/21/22 02:20 AM (1 year, 4 months ago) |
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, cool thread!
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Muad.Dweeb
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: hummingbird]
#27960332 - 09/21/22 08:42 AM (1 year, 4 months ago) |
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Thank you, everyone! I wish I could claim credit for any of this, but really I'm just pulling bits and pieces out of humanity's collective knowledge, and then doing work. I'm still working on both of these things, I'd like to make a much better inductor TBH, but I'll have to put research and testing time into the circuit and I'm not ready to commit to it atm.
The Dedi procedure I'm trying a lot of variants of still, and I'm moving out to other strains and species. I'm also preparing for an attempt at protoplast prep that would be reasonable for use in a hobby lab setting. So, exciting things coming up!
These things should be standard tools we all have the ability to use as needed.
-------------------- A beginning is the time for taking the most delicate care that the balances are correct. This every sister of the Bene Gesserit knows.
How to Breed like the Bene Gesserit The Weirding Way - Advanced Bene Gesserit Techniques
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Muad.Dweeb
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: Muad.Dweeb]
#27964133 - 09/23/22 11:00 AM (1 year, 4 months ago) |
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Update on the Dedi test plates:
As they continue growing, more macro-morphological differences are emerging. The A type has a partial tendency to grow asymmetrically, but regardless, it has a higher sensitivity to temperature changes (indicated by the concentric banding as room temp changes throughout the day). But the asymmetry is also in the local mycelial density, as illustrated by the third picture. Out of the 5 recovered A type, 2 display this asymmetrical, floccose behavior, plus the original A type recovered from a previous attempt.
  
The B type is very consistent, with pseudorhizomorphic striation in the colony pattern.

When the A/B plates are compared to either type, you can see the morphological distinctions. The pairing seems to consistently prefer increased development on the B side, with the A side not developing increased rhizomorphs or often size, significantly.
 
Compared to the A/A plates, B type again stands out, with the A/A colony preference falling to the more vigorous mycelium. The floccose A type gives way to the tomentose, circular A type. In some cases, it stops growing altogether, perhaps to allow growth from the more rigorous subculture to colonize the resources and support the weaker subculture, and increase the likelihood of an optimized pairing.
 
Looking now at the A/A and A/B plates compared to a control plate I allowed all the cultures to fuse on, we can see the A/B starting to resemble the rhizomorphs of the control plate.
 
Thanks for Watching!
I'll update with fruits of the re-paired culture and the original culture later when they fruit.
-------------------- A beginning is the time for taking the most delicate care that the balances are correct. This every sister of the Bene Gesserit knows.
How to Breed like the Bene Gesserit The Weirding Way - Advanced Bene Gesserit Techniques
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ReverendMyc

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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: Muad.Dweeb] 1
#27972389 - 09/28/22 02:34 PM (1 year, 3 months ago) |
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-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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Muad.Dweeb
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: ReverendMyc]
#27972615 - 09/28/22 05:09 PM (1 year, 3 months ago) |
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Pics were acting wonky yesterday, so I didn't post this update:
Three new cultures were selected for variations of the maceration technique- Gan.CL1 (a Gandalf clone), KS.CL1 (my favorite squat clone), and BLM.F3.CL2 (a lion's mane culture inbred from a wild N. China clone).
0.1mL LC of each were added to the same dex/pep/oster set up, and each blended 10 seconds. Incubation was for a total of 6 days. At 3 days, spheroid colonies were developing in the KS broth. Even at 6 days, Gan and BLM failed to culture. A 1cm sphere was retrieved from the KS broth and viewed at 1000x and determined to be fully monokaryotic. The remaining colonies were blended for 5 then another 5 seconds, with 101 dilutions made of each blend time [BT].

After thoroughly agitating, each BT was drawn into a 1mL syringe each, and 0.1mL was added to five water agar plates for both BT's, and each cross streaked for recovery of colonies. After retrieval, subcultures of each recovered neohaplont will be paired as before to determine nuclear type.
1 day after streaking, all 10 plates are showing growth, and 7/10 have colonies spatially isolated enough to transfer. I'll be circling them, inspecting them under the scope and transferring them to MEA later. Confirmed monos will then be paired, and the best representatives of nA and nB will be slanted. I'll try to remember to take pics.
Also, new broth will be sterilized and Gan/BLM will be attempted again with more care to ensure inoculum is introduced, and it is not over blended. Currently, I only have 3 blade bases, so I'm limited to 3 trials with this procedure at a given time.
-------------------- A beginning is the time for taking the most delicate care that the balances are correct. This every sister of the Bene Gesserit knows.
How to Breed like the Bene Gesserit The Weirding Way - Advanced Bene Gesserit Techniques
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Muad.Dweeb
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: Muad.Dweeb] 1
#27981032 - 10/04/22 11:28 AM (1 year, 3 months ago) |
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Trich Farming
intro to protoplast prepI have recently acquired the last pieces I think I'll need for conducting Protoplast Preparation. The "affordable, home lab" version necessitates more footwork to accomplish, but even with some lacking, I have already achieved spheroplasts (some cell wall remains, creating a "spheroid" with the protoplast inside) using both a Metarrhizum culture and a Penicillium culture (neither are ideal for full cell wall removal, but could have application similar to snake venom, since they are mainly proteolytic as I understand it...) However, some select extracts and a source of the preferred Trichodermas- harzianum and viride- are now in my possession, so I will be updating as I cultivate the trich, extract the enzymes (maybe show the precipitation of the enzymes, too, though it isn't necessary), lyse the cells, collect the protoplasts, and regenerate the protoplasts on agar. I'll use the premade extracts (used in brewing as Beta Glucanase and clarifying agents like "Clarity Ferm," respectively) in a side-by-side to see their efficacy as an alternative to having to cultivate mold. Up front, I expect the trichs to be far more effective, as between the two there is a full cell wall degrading complex that covers the glucans, the lipoproteins, and the chitin we need to get rid of. The specific cultures/enzymes used will be: - Bioglucanase TX (from Trichoderma reesei)
- Clarity Ferm protease (from Aspergillus niger; White Labs)
- Mykro-Root (T. harzianum and T. viride, 2x107 CFU/g; Microbial Applications)
* * *Simply to test the viability of the trich, I dabbed a swab into the package (in still air) and agitated the swab in a centrifuge tube with ~10mL of water. After mixing well, I added 0.1mL to 10mL of 1% LME broth and 0.1mL to an MEA plate, cross streaked. After 12 hours in distilled water, flecks of translucent white mycelia are visible when held to the light. There is no growth on the plate yet, and the LME clouds the broth too much to assess just yet. In order to ensure ease of cultivation, I will be trying three methods that are supposed to prevent the production of conidia (exponentially potentiating possibility of contam outbreaks). The first two I will relate here, since they are well known, and the third I will elaborate on later once I have another ingredient and have tested it a few times (it's experimental at the moment, and only theoretically applicable to trich). The two main methods of controlling sporulation of the molds are temperature control (higher temp incubation), and submerged cultivation (trich LC). Incubation Phase:Using LC is the easiest means of preventing mold spores. The addition of a stir bar/stir plate helps to ensure that the moving liquid keeps the culture submerged. While they can still easily produce oidia (shorter cell compartments that fragment off easily to increase asexual propagation), the nuisance conidiospores that go airborne don't form as readily. Heated incubation is typically not something we do in cultivation, but in this instance a reptile heat mat could potentially be used for constant temp heated incubation, provided it doesn't exceed the thermal limits of the fungi. Trichodermas can usually withstand 130-160 F (54-71 C), depending on species and strain. While 115 F (46 C) will be sufficient for mold cultivation, other, higher fungi will usually die around 110-115 F (43-46 C), so the preferred experimental range while generating the mold culture and for generating the enzymes is 90-100 F (32-37 C). Fermentation Phase:Now, for actually generating the enzymes, we have to stimulate the mold to produce them by feeding them what we want to break down later. In this case, liquid or solid medium with fungal tissue as the only food source, i.e. a mushroom powder broth or mushroom powder agar, or if you are feeling particularly sadistic, you can add the trich inoculum directly to colonized agar or LC with the target species of mushroom we will protoplast later. Allow 14 days to "ferment" the mycelium. If using LC, keep in mind that the final liquid volume should be minimal, as we cannot heat these to reduce the liquid without denaturing the enzymes (they begin degrading below 100 C, so boiling isn't an option). Collection and Purification:First, we need to separate all the fungal mass from the serum of water and enzymes. To do this, we will blend up the ferment (LC + Oster; or Agar + Water + Oster... remember to minimize the liquid), then do a coarse filter and a fine filter. So load up the oster jar (really, you can use any blender for this, as we purify it later) and blend it for at least 60 seconds. This is to kill any remaining fungi. It won't be 100% lethal, but enough to eliminate what minor risk remains in opening the jar. For the coarse filter, we need a funnel and some cotton, cheese cloth, polyfill, or similar to pack into the spout of the funnel. Filter through this, change the filter, then filter it at least once more. There will still be some solid particulate in this filtrate, and as an option, it can be refrigerated to help settle those particulates overnight, then decanted through the coarse filter again, leaving the sediment in the jar. The fine filtration will be done with a 0.22μm syringe filter (PES or acetate, NOT PTFE... hydrophobic filters don't like water), and be fairly slow. Don't apply too much pressure to the syringe as you filter it into a sterile container. Forcing it through can distort the membrane and allow impurities and contaminants to pass through. When the flow through the membrane decreases and the syringe plunger gets stiff, switch to a new filter and continue. The course filter (and overnight sedimentation) help immensely in reducing clogging of the filter, allowing more serum to be filtered before requiring changing the syringe filter. Light and heat are the main degrading components, so store in the fridge or freezer, and if desired, in amber glass, opaque bottles, or wrap in foil to block light. Label and date it as well. There is an additional step for refinement that can be taken that I haven't tried yet, as the chemicals aren't as easy as visiting a big box store to acquire. However, a precipitation step can be taken after fine filtration by adding potassium sulfate or magnesium sulfate to the serum, and chilling the serum. The precipitate can be collected on filter paper (coffee filter is fine), dried, and stored in glass in a refrigerator. This would be the closest to a quantitative recovery that would be measured and used around the same percentages of most published procedures for protoplast prep (typically 4-6% lysing agent). For use of serum, there will be more art and trial involved for getting the correct balance, as concentrations will be much lower. I have no idea what the shelf life of serum actually is, but I would hazard a guess that it is best used within 6 months, when stored in the proper conditions. Precipitated enzyme stored in the same conditions, perhaps a year.
-------------------- A beginning is the time for taking the most delicate care that the balances are correct. This every sister of the Bene Gesserit knows.
How to Breed like the Bene Gesserit The Weirding Way - Advanced Bene Gesserit Techniques
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Muad.Dweeb
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: Muad.Dweeb] 2
#27983235 - 10/05/22 05:43 PM (1 year, 3 months ago) |
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I can't upload this because of data limits, but I spent the day watching trich grow:
https://imgflip.com/gif/6vw0jg[animated .GIF]
I find it especially interesting how the old growth forms the cross-bar, fat hyphae to support aerial growth. I expect there will be condia to help me ID which is which soon.
Isn't she beautiful?
-------------------- A beginning is the time for taking the most delicate care that the balances are correct. This every sister of the Bene Gesserit knows.
How to Breed like the Bene Gesserit The Weirding Way - Advanced Bene Gesserit Techniques
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 11 days, 8 hours
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: Muad.Dweeb]
#27983301 - 10/05/22 06:16 PM (1 year, 3 months ago) |
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Intimidating and beautiful at the same time.
-------------------- Willpower is the one true virtue
  
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DERRAYLD
Constructus


Registered: 05/13/02
Posts: 9,281
Loc: South Africa
Last seen: 6 hours, 17 minutes
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: Muad.Dweeb]
#27983966 - 10/06/22 02:30 AM (1 year, 3 months ago) |
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Some beautiful work
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mind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: DERRAYLD]
#27984261 - 10/06/22 09:13 AM (1 year, 3 months ago) |
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-------------------- Mind's Easy Bag 2 Bag Grain Transfers Endless Sub Tek ...the doll's trying to kill me and the toaster's been laughing at me...
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Muad.Dweeb
Seer



Registered: 04/13/17
Posts: 148
Last seen: 5 months, 7 days
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Re: The Weirding Way - Advanced Bene Gesserit Techniques [Re: mind.at.large]
#27988643 - 10/08/22 04:23 PM (1 year, 3 months ago) |
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Cultivating trich is going to be... still harder than I thought.
I did not account for the fact that this Mykro-Root is a horticultural supply, and gets mixed into dirt, so no consideration for sterile manufacture or packaging was made, and that beautiful trich from the .gif is overrun with a pinmold now. The six plates I made from the streak plate are pinmold (maybe with trich hiding at the center, but effectively it is just pinmold, given how fast they grow).
But God doesn't spit in your face without cupping your balls, or something, and an old spent tub that I didn't toss finally seems to have attracted trich. So I'm trying to propagate that, and I'll return to serial diluting the Mykro-Root and seeing if I can get clean isolations. At the least, it doesn't appear that this is a germ cocktail, just an unclean product.
Wish me luck! I might burn through a few hundred plates before I update this particular topic.
-------------------- A beginning is the time for taking the most delicate care that the balances are correct. This every sister of the Bene Gesserit knows.
How to Breed like the Bene Gesserit The Weirding Way - Advanced Bene Gesserit Techniques
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