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Offlineluteofolius 2.0
Mycologist
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Registered: 10/16/22
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Re: Symbiotic liverwort for Golden Teachers - the hunt for moldless cultivation [Re: luteofolius 2.0]
    #28003061 - 10/17/22 12:31 PM (1 year, 3 months ago)

If you Google the discovery of penicillin you will see that we humans have known about antibiotics for quite some time.  However use in our field is relatively new as in the last 50 60 years


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Offlinerumfor69
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Re: Symbiotic liverwort for Golden Teachers - the hunt for moldless cultivation [Re: luteofolius 2.0]
    #28003104 - 10/17/22 01:18 PM (1 year, 3 months ago)

Bacteria in agar is so frickin easy to get rid of through technique alone without any of all that stuff. Just a waste of time and money imo lol


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Invisiblebakedbeings
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Re: Symbiotic liverwort for Golden Teachers - the hunt for moldless cultivation [Re: rumfor69]
    #28003125 - 10/17/22 01:32 PM (1 year, 3 months ago)

:whathesaid:


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Offlineluteofolius 2.0
Mycologist
I'm a teapot
Registered: 10/16/22
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Re: Symbiotic liverwort for Golden Teachers - the hunt for moldless cultivation [Re: rumfor69]
    #28004575 - 10/18/22 11:48 AM (1 year, 3 months ago)

It works really good for cloning wild specimens although you are right that it can be avoided, I find it helpful in certain conditions


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OfflineCrashexx
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Re: Symbiotic liverwort for Golden Teachers - the hunt for moldless cultivation [Re: luteofolius 2.0]
    #28004792 - 10/18/22 02:18 PM (1 year, 3 months ago)

Quote:

luteofolius 2.0 said:
It's funny that usnea is being brought up.  I'm actually friends with someone that is trying to extract usnic acid for medical use, and the first thing I thought of was: can I powder it and put in in agar to ward off bacteria?  I guess to answer the ops question again I would say that we already use antibiotics created from organisms as an aid in fungi culture, for example  Gentamicin is naturally produced by the bacterium Micromonospora purpurea and is used in fungi culture




I have been using usnea medicinally for a while, and today I brought home some Xerocomellus that I would like to try to grow on agar - it might respond well to usnea. Will make a batch of usnea agar (and maybe some varieties with different parts of oak?) as soon as I get to it and report about results.

Where I live I'd use the usnea for two reasons: availability and and possible use for mycoremediation and establishing mycorrhizal fungi after wildfire.
To get an antibiotic I'd have to drive to town, or order stuff, and spend money and transport energy. Usnea and other liquen on the other hand grow in front of my doorstep.

Another plant I'll start experimenting with is cytisus monoflorus. Hillsides all over are covered with them after wildfire, and they'd make a cheap and easily available substrate. I plan to build some outdoor patches out of cytisus and horseshit - which I also happen to have. If you know of any useful info about that, please point me towards it.


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Offlineluteofolius 2.0
Mycologist
I'm a teapot
Registered: 10/16/22
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Re: Symbiotic liverwort for Golden Teachers - the hunt for moldless cultivation [Re: Crashexx]
    #28005544 - 10/18/22 09:18 PM (1 year, 3 months ago)

I dont know directly 8f scotch broom would be a good candidate for mushroom substrate.  I will say that I have witnessed various species of Galerina growing from the dead stalks of some scotch broom in my locality.    Scotch broom sure has a lot of thick branches that would pulp nicely in a woodchipper. But there are a few things I don't know about it that may not work out.  Maybe it conta8is something like conifer trees where in that fungi might not accept the medium.  It would be worth a test. 


It would be smart to chop them down, let them dry somewhere and hope they don't mold, let's say cure them in a way, and then chop it up fine.  Mix that with your horse shit and I would imagine that to be full of lignin and also nitrogen from the horse shit.    Not only that but scotch room is rather invasive to native plants so if you could find a use for it you would be helping mother nature out. 

I like that idea.


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OfflineBoodoo
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Re: Symbiotic liverwort for Golden Teachers - the hunt for moldless cultivation [Re: Listed]
    #28226678 - 03/12/23 05:20 PM (10 months, 11 days ago)

From OpenAI:

"Pseudomonas putida is a type of bacteria that can form a beneficial relationship with Psilocybe cubensis. Studies have shown that Pseudomonas putida can enhance the growth of Psilocybe cubensis under laboratory conditions by improving nutrient availability and increasing the bioavailability of organic compounds.

One study found that when Pseudomonas putida was co-cultured with Psilocybe cubensis, the bacterial cells colonized the surface of the fungal mycelium and produced organic acids and other compounds that increased the availability of nutrients, such as nitrogen and phosphorus, for the fungus. The presence of Pseudomonas putida also led to an increase in the production of some secondary metabolites, such as psilocybin, which is the psychoactive compound found in Psilocybe cubensis.

Overall, the relationship between Pseudomonas putida and Psilocybe cubensis is thought to be a mutualistic one, in which the bacteria provides essential nutrients and growth factors to the fungus in exchange for organic compounds and other nutrients. However, it is important to note that the exact nature of this relationship and its effects on the growth and development of Psilocybe cubensis in nature is not well understood and requires further research."

Not sure about it's availability for you though. It also said it forms relationship with plant roots but this will be to supplement other food sources in nature.


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Offlineichnoguy
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Registered: 10/27/22
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Last seen: 2 months, 7 days
Re: Symbiotic liverwort for Golden Teachers - the hunt for moldless cultivation [Re: luteofolius 2.0]
    #28245577 - 03/25/23 03:42 AM (9 months, 29 days ago)

Quote:

luteofolius 2.0 said:
I hate to say it but the only difference between an antifungal and an antibiotic is dosage when we are explicitly referring to natural antibiotics created by imperfect fungi or plants.    Antibiotics do kill mold as well.    However bacteria don't have the tolerance for it that fungi do and I assume that's due to how bacteria are built if you will.



yes for some things, bacteria need the lower dose and virus need the stronger one than kills fungus?

but a some are selective, so probably a big problem that we dont know anything about is viruses.

To op its a cool idea i think you can find benefitial organism if you test the substrates of healthy mushrooms in the wild. Check the tissue on the outside of the mushrooms, biodiversity study of healthy and unhealthy environments etc everything has its symbiots


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