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Offlinehazyhorse
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
    #27977379 - 10/02/22 12:02 AM (2 years, 3 months ago)

yeah for sure!! you just wanna make sure you don’t touch the inside & also don’t use anything to cut into it bc by cutting in you’ll drag potential contaminates in from the surface of the stipe. if you can tear it a bit to get to fresh tissue & then use a sterile scalpel to get a little you should be fine


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Offlinerumfor69
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
    #27977381 - 10/02/22 12:06 AM (2 years, 3 months ago)

Little pins like that grow extremely fast in that part of their growth cycle. They turn to mycelium in a plate in a matter of a few days and will beat out contams most time.

I stab them with a fire hot safty pin, rip them off the sub, then slide off the safety pin onto a plate with a sterile scale or another flamed safety pin. Then transfer mycelium that grows out from the cap end of the pin on the agar.

But I've done this a lot so it sounds easy to me lol center flesh can be a ton cleaner.


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Offlineechochild
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Re: High Humidity in Monotub - is this an issue? [Re: rumfor69]
    #27977389 - 10/02/22 12:16 AM (2 years, 3 months ago)

Quote:

rumfor69 said:
Little pins like that grow extremely fast in that part of their growth cycle. They turn to mycelium in a plate in a matter of a few days and will beat out contams most time.

I stab them with a fire hot safty pin, rip them off the sub, then slide off the safety pin onto a plate with a sterile scale or another flamed safety pin. Then transfer mycelium that grows out from the cap end of the pin on the agar.

But I've done this a lot so it sounds easy to me lol center flesh can be a ton cleaner.




Rumfor, that sounds like it needs a practiced hand at it LOL! I think I'll see if that fatty pin decides to get fatter and go or a center cut.

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Offlinehazyhorse
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
    #27977391 - 10/02/22 12:20 AM (2 years, 3 months ago)

ya pins colonize hella fast for sure & i absolutely love taking pins from agar plates if possible. i just try to be as sterile as possible when it comes to cloning & if it’s a last ditch effort coming from a tub w an active contam i’d personally wanna make it as safe as possible but pins are super vigorous on agar regardless, just depends on how willing you are to take chances!

you can always try cloning a few pins with different methods to see how it plays out


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OfflineRusty2096
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
    #27977621 - 10/02/22 07:56 AM (2 years, 3 months ago)

Maybe. But why?

Why make your life harder when you can do it on a nice looking mature fruit?

And with that pin, maybe it was about to grow small and weak, you don't know. Why clone a pin here?


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Offlinerumfor69
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Re: High Humidity in Monotub - is this an issue? [Re: Rusty2096]
    #27977630 - 10/02/22 08:00 AM (2 years, 3 months ago)

Rusty has a good point here. If this is your only clone attempt and you have nothing else going then it'd be best to select a mature fruit that has the characteristics you like.

I have bunches of different cultures and clones and mss things going so I grab pins and plop em on dishes cause I don't care if it works or not. With my main cultures that are the important staple of my work I don't mess around like that.


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Offlineechochild
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Re: High Humidity in Monotub - is this an issue? [Re: Rusty2096]
    #27977765 - 10/02/22 09:32 AM (2 years, 3 months ago)

Quote:

Rusty2096 said:
Maybe. But why?

Why make your life harder when you can do it on a nice looking mature fruit?

And with that pin, maybe it was about to grow small and weak, you don't know. Why clone a pin here?




Maybe I'm using the wrong terminology? It's not quiet a pin, but an immature mushroom? Pins are very small, like only a few mm tall right? This one looks to be a few centimeters at this point.

My only reason for thinking of this, was in case that weird fuzzy area began to take over the tub, since I have no agar backups of this strain. I would prefer a mature mushroom for this to be sure.

But, as far as this morning goes, I don't see the weird area spreading so far, but I also didn't notice any growth from the pins directly in that area either (but it also has only been 8 ish hours since I looked last night).

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Invisiblebakedbeings
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
    #27977770 - 10/02/22 09:34 AM (2 years, 3 months ago)

just let things play out. you can still get clean tissue from a fruit thats surrounded by mold


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Offlineechochild
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Re: High Humidity in Monotub - is this an issue? [Re: bakedbeings]
    #27977781 - 10/02/22 09:40 AM (2 years, 3 months ago)

Quote:

Bakedbeings said:
just let things play out. you can still get clean tissue from a fruit thats surrounded by mold




Agreed. Mostly this was a hypothetical conversation. Mostly I was just curious if it could be done, more than any desire to do it. :smile:

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Offlineechochild
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
    #27979211 - 10/03/22 09:14 AM (2 years, 3 months ago)

Ok, so some update photos. I don't think that weird area is spreading, but I also don't see a lot of growth in the pins in that area. There is also an area where pins don't really seem to be growing in the opposite area of the tub.

With Flash:


Without Flash

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Invisiblebakedbeings
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
    #27979215 - 10/03/22 09:17 AM (2 years, 3 months ago)

lets focus on the positives...all ya need is one fruit, and i see several ones


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Offlineechochild
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Re: High Humidity in Monotub - is this an issue? [Re: bakedbeings]
    #27979227 - 10/03/22 09:24 AM (2 years, 3 months ago)

Quote:

Bakedbeings said:
lets focus on the positives...all ya need is one fruit, and i see several ones




I'm just happy I have gotten this far, so far. This is the furthest I have been down this track. :smile:

Edit: Especially considering I kind of messed it all up in the beginning with noob-ness.

Edited by echochild (10/03/22 09:27 AM)

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Offlinerumfor69
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
    #27979242 - 10/03/22 09:37 AM (2 years, 3 months ago)

Yep this is win really. There's not much at all you can do really to help or fix anything. The goal now is to take some prints or swabs. And clone a bigger fruit from a cluster to agar or a brf puck.


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Offlineechochild
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Re: High Humidity in Monotub - is this an issue? [Re: rumfor69]
    #27980778 - 10/04/22 07:49 AM (2 years, 3 months ago)

Yeah, I'll take a win where I can. So, just so I know, these suckers are getting quite long, but the stipes look thick. This means I have proper FAE right, or do I need to take some polyfil out?



Also, we are now off to the races on the first batch of my PF Tek jars. Wish me luck.

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Invisiblebakedbeings
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Re: High Humidity in Monotub - is this an issue? [Re: echochild] * 1
    #27980805 - 10/04/22 08:10 AM (2 years, 3 months ago)

they look happy


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OfflinePBJ710
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Re: High Humidity in Monotub - is this an issue? [Re: bakedbeings]
    #27980822 - 10/04/22 08:19 AM (2 years, 3 months ago)

:whathesaid:

Don't worry about having a little velvet on the stipes as long as you're maintaining ideal surface conditions.  I have a couple cultures that do that to some extent no matter how much FAE I give them.

I would wait for that cluster in the middle of the pic to mature a bit more and take clones from the biggest fruit in it.

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Offlineechochild
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Re: High Humidity in Monotub - is this an issue? [Re: bakedbeings]
    #27980837 - 10/04/22 08:29 AM (2 years, 3 months ago)

Quote:

Bakedbeings said:
they look happy




Thanks Baked :smile: I'm a little worried about trying to fit them in mason jars in the future without cutting them down a bit.

Quote:

PBJ710 said:
I would wait for that cluster in the middle of the pic to mature a bit more and take clones from the biggest fruit in it.




Yeah, I was watching that cluster too for the same intent. How much more time do you think it will be until they are ready to pick? Should the tops flatten out a little more?

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Offlinerumfor69
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Re: High Humidity in Monotub - is this an issue? [Re: echochild]
    #27980894 - 10/04/22 09:23 AM (2 years, 3 months ago)

Look under them with a little compact mirror. I use a dental mirror. Then you can see if the veils are starting to tear or micro tear.


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OfflinePBJ710
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Re: High Humidity in Monotub - is this an issue? [Re: rumfor69]
    #27981006 - 10/04/22 11:16 AM (2 years, 3 months ago)

If it's your first flush, I say let them open up a bit so you can get spore prints out of them as well as clones.  For future flushes where you don't care about prints harvest them while veils are still attached so they will store alot better.

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Offlineechochild
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Re: High Humidity in Monotub - is this an issue? [Re: rumfor69]
    #27981285 - 10/04/22 02:11 PM (2 years, 3 months ago)

Quote:

rumfor69 said:
Look under them with a little compact mirror. I use a dental mirror. Then you can see if the veils are starting to tear or micro tear.




I ended up using a very shiny butter knife :laugh:

Quote:

PBJ710 said:
If it's your first flush, I say let them open up a bit so you can get spore prints out of them as well as clones.  For future flushes where you don't care about prints harvest them while veils are still attached so they will store alot better.




I was out of the house for a few hours and came back and 4 of them have already broken their veils. I harvested the big one from that cluster and took 3 live samples from inside and have the cap sitting now for a spore sprint.

The biggest one so far was 5 inches long and 14.5 g. In all the times I have tried mushrooms in the past, I have never seen them this big. Is this normal?

I've put the ones I harvested into a paper bag in the fridge. I got a big mason jar, but I need to order desiccants for it. I have a drying kit I will prolly throw them into.

What I was wondering is this: Seems the babies grew up at wildly differing speeds. Should I also weight for the veils to tear on the other 80% before I harvest? What should I do with the little ones that still look like they are growing slowly? I should pick the aborts now, right?

I need to get a fine mister. The sub is looking a bit dry in certain places.

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