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Onlinechris77
Archaic Revivalist
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Registered: 01/17/22
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Re: Psilocybe natalensis [Re: Guerrilla]
    #27947746 - 09/13/22 03:46 AM (1 year, 5 months ago)

thank you for clearing this up. i easily get confused about correct nomenclature


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the observer is the observed
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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: chris77] * 2
    #27947768 - 09/13/22 04:32 AM (1 year, 5 months ago)

Try to figure out what a monoculture is, I dare you :lol:



There’s definitely a lot of specific lingo we use and sometimes a lot of it isn’t clear or agreed upon even.


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LAGM2020


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InvisibleGuerrilla
Bumbaclart

Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom Flag
Re: Psilocybe natalensis [Re: A.k.a]
    #27947787 - 09/13/22 05:36 AM (1 year, 5 months ago)

"A monoculture refers to the presence of a single crop/organism so all axenic cultures are a monoculture; and an isolate is either a single germinated spore that produces a single monokaryotic hypha, or a single strain that is the result of two mated pair of individual hyphae emerging from 2 distinct spores."

Well yeah shit, my understanding was that a monoculture was the pairing of two individual monokayrons. That's why I deleted my original post, I knew it was a fucking wormhole.

And even after reading that I still don't really consider an isolate to be any axenic culture, though I understand why that's the case.

So I get what you mean about stuff not being agreed on.


Edited by Guerrilla (09/13/22 06:19 AM)


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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: Guerrilla] * 1
    #27947831 - 09/13/22 07:03 AM (1 year, 5 months ago)

Lmao yeah the whole mono/isolate thing is insane. There’s a couple terms like that where people use them to mean different things.

At least people don’t refer to spawning a tub as “casing” anymore.


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LAGM2020


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,234
Last seen: 51 minutes, 7 seconds
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Re: Psilocybe natalensis [Re: A.k.a] * 1
    #27947832 - 09/13/22 07:10 AM (1 year, 5 months ago)

Quote:

A.k.a said:

At least people don’t refer to spawning a tub as “casing” anymore.





Right! we "plant" our spawn


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All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineDERRAYLD
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Re: Psilocybe natalensis [Re: Camera93] * 4
    #27947834 - 09/13/22 07:12 AM (1 year, 5 months ago)

Yes we plant our spawn in tubes


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OfflineBlueAndOrange
Psychedelic success story…
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Registered: 01/11/22
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Re: Psilocybe natalensis [Re: DERRAYLD] * 1
    #27947941 - 09/13/22 09:23 AM (1 year, 5 months ago)

Alright. So I’m a noob. I’m in the middle of my first flush with nats. I’m interested in trying to contribute to taking and pheno selection.

Right now I’m sitting on 2.25 jars of fully colonized milo with bay mycelium, and 21 jars of newly G2G inoculated  milo. Heading to the store to pick up bags of grain today and start prepping at least 40 more jars. I’m looking at growing some real bulk of these nats this fall.

So I’m hoping I’ll have a lot to choose from for purposes of cloning and trying to stabilize some phenotypes. I’ve still never gotten a successful tissue sample to generate mycelium on agar. If this is something that I’m really determined to do, does anyone have any tips for being successful cloning nats?

Specific agar recipes? My failed techniques in the past involved LME and charcoal agar. This time I’m going LME and nutritional yeast and a little blue food color (and some with no food color).

As for tissue sampling, I have torn the stipes and scraped the tissue from the centers of the stipes. Is there a better way?

Hopefully I can start moving towards a stabilized pheno over the next few years.

Perhaps we should start a thread for those of us looking to tame nats… I’d love to see some TCs join in on the party.


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OfflineDERRAYLD
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Re: Psilocybe natalensis [Re: BlueAndOrange] * 4
    #27947948 - 09/13/22 09:27 AM (1 year, 5 months ago)

Scientifically speaking..... I ripped this fucker open, flamed the blade and made a transfer just like agar.

No issue whatsoever, multiple clean plates with healthy growth.



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OfflineBlueAndOrange
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Re: Psilocybe natalensis [Re: DERRAYLD]
    #27947953 - 09/13/22 09:29 AM (1 year, 5 months ago)

Nice. Hopefully I’ll get to a point where I’m building a nat mycelium library of super potent thick stemmed monsters. :smile:


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InvisibleBigwormS
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Registered: 03/19/22
Posts: 1,902
Loc: Murica Flag
Re: Psilocybe natalensis [Re: BlueAndOrange]
    #27947983 - 09/13/22 09:52 AM (1 year, 5 months ago)

Quote:

BlueAndOrange said:

As for tissue sampling, I have torn the stipes and scraped the tissue from the centers of the stipes. Is there a better way?

Hopefully I can start moving towards a stabilized pheno over the next few years.

Perhaps we should start a thread for those of us looking to tame nats… I’d love to see some TCs join in on the party.




I don't scrape the tissue, I cut a small section about a mm long and then spice under it to pull a small section out. Scraping my damage the tissue too much, but I'm no expert. I usually see growth in 12-24 hours of fuzz on the piece.


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OfflineSpicolis_Dad
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Registered: 08/24/20
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Re: Psilocybe natalensis [Re: Spicolis_Dad] * 1
    #27948059 - 09/13/22 11:24 AM (1 year, 5 months ago)

Quote:

Spicolis_Dad said:


3 weeks since inoculation and 1 week post shake #1. The agar wedges I used to 'noc these were from two different T1 plates.  Each plate had a "slow" side and a "fast" side and I took a wedge from each of the two sections from each plate.  The colonization of the jars is reflecting this as I now have 1 jar at ~97%, 1 at maybe 90%, and the two "slower" jars are each at about 65%. I plan to fruit in 4 shoe-boxes in a large tub.  My question is, should I spawn each jar as it comes ready or wait until they're all at 100% colonization before spawning?




The bare spot on the jar on the left was covered this morning so I spawned it to 100% coir (bucket tek) at a 1:3 ratio. 2nd jar from left will likely be ready to go tomorrow.  Not sure about the other 2, looked back through pictures and they're growing, just quite slowly.

This is exciting!


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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: Spicolis_Dad] * 7
    #27948081 - 09/13/22 11:42 AM (1 year, 5 months ago)

Just pulled the rest of the second flush, here’s some of the more unique ones.




Gonna clone one of those two smaller roundish ones.


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InvisibleZifozonke
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Re: Psilocybe natalensis [Re: A.k.a]
    #27948116 - 09/13/22 12:21 PM (1 year, 5 months ago)

Nat Envy...
Aka them be some fine looking fatties
:casket::casket::casket:


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Offlinelabbar
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Re: Psilocybe natalensis [Re: Zifozonke] * 5
    #27948213 - 09/13/22 01:51 PM (1 year, 5 months ago)

MelNat:laugh2:



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InvisibleEgon_Spengler
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Registered: 04/02/22
Posts: 2,294
Re: Psilocybe natalensis [Re: labbar] * 3
    #27948289 - 09/13/22 02:47 PM (1 year, 5 months ago)



tiger drop some nice T3’s today, first Nats to grain!


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OnlineYoshiTrainer
Onion tied to belt
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Re: Psilocybe natalensis [Re: Egon_Spengler]
    #27948326 - 09/13/22 03:09 PM (1 year, 5 months ago)

3lb bag of WBR and pearled barley started on 6/17/22. I'm hoping to let it run for a year or until it fruits to test the mycelium strength.



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OfflineSpicolis_Dad
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Re: Psilocybe natalensis [Re: Spicolis_Dad]
    #27949464 - 09/14/22 10:20 AM (1 year, 5 months ago)

Quote:

Spicolis_Dad said:
Quote:

Spicolis_Dad said:


3 weeks since inoculation and 1 week post shake #1. The agar wedges I used to 'noc these were from two different T1 plates.  Each plate had a "slow" side and a "fast" side and I took a wedge from each of the two sections from each plate.  The colonization of the jars is reflecting this as I now have 1 jar at ~97%, 1 at maybe 90%, and the two "slower" jars are each at about 65%. I plan to fruit in 4 shoe-boxes in a large tub.  My question is, should I spawn each jar as it comes ready or wait until they're all at 100% colonization before spawning?




The bare spot on the jar on the left was covered this morning so I spawned it to 100% coir (bucket tek) at a 1:3 ratio. 2nd jar from left will likely be ready to go tomorrow.  Not sure about the other 2, looked back through pictures and they're growing, just quite slowly.

This is exciting!




2nd jar from left in above pic is 100% today so off to spawn we go. Pics below are some cool rhizo growth in 2nd and 3rd from left in top pic.
   


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InvisibleGuerrilla
Bumbaclart

Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom Flag
Re: Psilocybe natalensis [Re: Spicolis_Dad] * 4
    #27949591 - 09/14/22 11:46 AM (1 year, 5 months ago)

Quote:

bodhisatta said:
The species was described as new to science in 1995 by
Jochen Gartz,

Lol 99.95% chance it's not even a new species. This guy and Paul are the absolute kings of bad taxonomy and trying to make new species.

Someone needs to get Alan a sample to put it to rest




Every time I read this I get a little chuckle


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Being pissed on does not make you a real man.


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InvisibleNot Quite Social
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Registered: 07/16/10
Posts: 1,418
Loc: Midwest
Re: Psilocybe natalensis [Re: Guerrilla] * 3
    #27949662 - 09/14/22 12:36 PM (1 year, 5 months ago)

This tub was spawned on 9/6, and I think it's looking pretty good.

Seems to like the thick pseudo casing.





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Offlinesmalltalk_canceled
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Re: Psilocybe natalensis [Re: Guerrilla]
    #27950023 - 09/14/22 04:58 PM (1 year, 4 months ago)

Honestly cubes don't throw the fan formations or make the mega blobs in the same way in that regard they feel truly different, ill accept evidence towards the contrary but for now revel in the better cube, Natalensis.
Of African descent


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Willpower is the one true virtue



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