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monkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 5 months, 24 days
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Quote:
Smellyhobbit said:
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monkey_accident said:
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Smellyhobbit said: Learned I don’t have experience enough to make and use LC yet. Had a plate that looked clean, chopped it up, threw it in some broth. Growth looked great. Posted it here, got good feedback. Instead of testing it on a Petri, since I lost all my plates to contam, I just made a few quarts of millet and noc’d it up. Figure that’s as good as a plate if you’re just seeing if it’s contaminated.
Boy was it contaminated. All I grew was some sweet smelling green mold. This stuff looks identical to pictures of healthy LC here on shroomery, but it was totally worthless. I suppose my plate wasn’t as clean as I thought, but if it wasn’t then it really fooled me.
Anyway, I haven’t had back luck inoculating with agar to grain, so I’m just going to keep doing that. LC might save a little time, but if you do new grain every week it hardly matters.
iirc you don't use a FH? honestly not recommended doing LC without one, josex poke could help you out tho sorry for the loss 
It’s ok. Maybe that’s really the answer, waiting until I have a solid lab. I find it, so far, pretty easy to clean agar under a SAB as long as you know you have good plates. I’ve cleaned up a handful in short order since getting my agar working.
I love the idea of liquid culture. But for now I’m very comfortable with not having to introduce several more vectors for contamination into an already clean workflow. A2G is perfectly serviceable.
yeah i agree - do what works for you anything can be done in a SAB so don't get me wrong it just seems most people get hit or miss results with LI in a SAB
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: 🌌
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I vote try again. Next time just test it on agar for a few days prior to inoc.
Also, I suggest to inoc your LC with only a small piece of agar to reduce tam chances. Like a transfer - small piece of leading edge. Yes, it will take longer for your LC to grow but is that so bad?
I'm new to this, I work in a SAB and I also failed my first LC. Second one was legit.
In 2 days (waiting for more growth on plates) I'm trying SSLC.
If I can do it, you sure can friend ☺
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,602
Loc: Hole
Last seen: 7 minutes
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Re: Shoebox, to progress? [Re: Inocuole]
#27944047 - 09/10/22 04:49 PM (1 year, 5 months ago) |
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If I make more, which I may after I think about it for a few days lol, it’s going to be with a smaller piece. I’m not worried about time. I’ve got the patience for it. I used basically a tiger drop for this LC, so that obviously increased my chances of contam.
Once I order up a proper box of plates I won’t worry so much about using them all up.
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monkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 5 months, 24 days
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Re: Shoebox, to progress? [Re: Rusty2096]
#27944051 - 09/10/22 04:51 PM (1 year, 5 months ago) |
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try and try again that is de way
im in the boat of not testing LC - hear me out opening my LC to test it is just another vector for contamination you can 'test' an LC and the growth be clean - but during your test you may contaminated it unknowingly
to each their own though
edit: tiger dropping ur first LC.. u got some balls
Edited by monkey_accident (09/10/22 04:52 PM)
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,852
Loc: In the Gills
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Re: Shoebox, to progress? [Re: Rusty2096] 1
#27944054 - 09/10/22 04:52 PM (1 year, 5 months ago) |
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I have 2 flowhoods and I dont do LC cause I don't get perfection as is so I figured why compound that with something I cant visually see if it's clean to use. Like you said doing weekly cycles eliminates it's need. I make up batches of master jars A2G then store them in a mini fridge at 45°f until I need them for G2G.
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Rusty2096
rah rah raw in Lady gaga



Registered: 08/23/22
Posts: 4,946
Loc: 🌌
Last seen: 23 days, 14 hours
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I hear you. I'm making LC purely to improve my skills. I have no need for faster/larger scale colo. I'm that kind of person who needs to fail to learn, push my limits (in everything I do not only LC loll)
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,602
Loc: Hole
Last seen: 7 minutes
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Re: Shoebox, to progress? [Re: Rusty2096]
#27944064 - 09/10/22 04:58 PM (1 year, 5 months ago) |
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Quote:
Rusty2096 said: I hear you. I'm making LC purely to improve my skills. I have no need for faster/larger scale colo. I'm that kind of person who needs to fail to learn, push my limits (in everything I do not only LC loll)
Failing to learn is my style too, friend. 100%. I am very rarely upset with a failure. I got a little butthurt when I lost all those blank plates, but that’s only because I couldn’t learn from it. But it did make me more cautious with everything going forward, so it wasn’t totally valueless.
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Smellyhobbit
Actual Retard



Registered: 04/01/22
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Re: Shoebox, to progress? [Re: rumfor69]
#27944070 - 09/10/22 05:01 PM (1 year, 5 months ago) |
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Quote:
rumfor69 said: I have 2 flowhoods and I dont do LC cause I don't get perfection as is so I figured why compound that with something I cant visually see if it's clean to use. Like you said doing weekly cycles eliminates it's need. I make up batches of master jars A2G then store them in a mini fridge at 45°f until I need them for G2G.
How many expansions do you do from G2G? Do you set aside one of your receivers as a new master every time; and if so how many times are you comfortable repeating that?
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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
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Re: Cultivation General Discussion [Re: Smellyhobbit] 2
#27944131 - 09/10/22 05:34 PM (1 year, 5 months ago) |
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Quote:
Smellyhobbit said: If I make more, which I may after I think about it for a few days lol, it’s going to be with a smaller piece. I’m not worried about time. I’ve got the patience for it. I used basically a tiger drop for this LC, so that obviously increased my chances of contam.
Once I order up a proper box of plates I won’t worry so much about using them all up.
If you test an LC, while you're practicing, and it doesn't come back 100% clean on a plate.. you can still send it to PF Tek so you still get something for your work and it's not a complete loss..
I haven't tested this jar yet, but I plan to send it in a day or 2.. I've had a 100% success rate sending LC to cakes,.. just food for thought.

Edit: changing the subject line back
Edited by Rotnpins (09/10/22 05:35 PM)
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milkboy
Child



Registered: 05/01/21
Posts: 2,318
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Re: Cultivation General Discussion [Re: Rotnpins] 2
#27944136 - 09/10/22 05:38 PM (1 year, 5 months ago) |
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Quote:
Rotnpins said:

Whats in the bag?
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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
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Re: Cultivation General Discussion [Re: milkboy] 2
#27944145 - 09/10/22 05:46 PM (1 year, 5 months ago) |
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Quote:
milkboy said:
Quote:
Rotnpins said:

Whats in the bag?

Phobos
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milkboy
Child



Registered: 05/01/21
Posts: 2,318
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Re: Cultivation General Discussion [Re: Rotnpins] 1
#27944150 - 09/10/22 05:49 PM (1 year, 5 months ago) |
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,852
Loc: In the Gills
Last seen: 1 day, 2 hours
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Quote:
Smellyhobbit said:
Quote:
rumfor69 said: I have 2 flowhoods and I dont do LC cause I don't get perfection as is so I figured why compound that with something I cant visually see if it's clean to use. Like you said doing weekly cycles eliminates it's need. I make up batches of master jars A2G then store them in a mini fridge at 45°f until I need them for G2G.
How many expansions do you do from G2G? Do you set aside one of your receivers as a new master every time; and if so how many times are you comfortable repeating that?
I don't do more expansions. I'll make 18 masters, store them in a fridge, use 2 a week to g2g to receivers, make tubs. When I'm down to 8 left in the fridge I make another 18.
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,602
Loc: Hole
Last seen: 7 minutes
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Re: Shoebox, to progress? [Re: rumfor69]
#27944169 - 09/10/22 05:56 PM (1 year, 5 months ago) |
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Excellent. I love that. I think I’ll try modeling my workflow similarly. Thank you.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,852
Loc: In the Gills
Last seen: 1 day, 2 hours
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Quote:
Smellyhobbit said: Excellent. I love that. I think I’ll try modeling my workflow similarly. Thank you.
Soon I'll be testing these things so the underband area of jars and dishes can remain sterile or at least super clean.
https://www.shroomery.org/forums/showflat.php/Number/27940004#27940004
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,602
Loc: Hole
Last seen: 7 minutes
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Re: Shoebox, to progress? [Re: rumfor69]
#27944185 - 09/10/22 06:08 PM (1 year, 5 months ago) |
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Quote:
rumfor69 said:
Quote:
Smellyhobbit said: Excellent. I love that. I think I’ll try modeling my workflow similarly. Thank you.
Soon I'll be testing these things so the underband area of jars and dishes can remain sterile or at least super clean.
https://www.shroomery.org/forums/showflat.php/Number/27940004#27940004
Yes I saw that. I’m interested to hear what happens
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wild_aussie
Punk rocker til I die



Registered: 01/05/22
Posts: 1,000
Loc: Skatepark grinding hard
Last seen: 10 days, 16 hours
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Re: Shoebox, to progress? [Re: Inocuole] 1
#27944486 - 09/10/22 09:08 PM (1 year, 5 months ago) |
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Hey smellyhobbit a quick chime in from a noob close to your experience level. Last weekend I noc'ed up 8 jars g2g, 6 lc 10ml per jar, 7 lc 5ml and this morning they all got there final shake and going to spawn tomorrow. So there isn't a big difference in time saved between the with this one run as my only example lol 🤣🤣. Now that I switch to wbs and my jars are gettin it now I'm all about the g2g especially using a sab like you. So there is 2 of us at least that are going that way. I've had a couple clean lc's but after first jars clean enough to try g2g I'm sold hands down
-------------------- BEWARE OF PICK POCKETS AND LOOSE WOMEN!! "Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!" Hunter S. Thompson "I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse " Sticking feathers up your ass does not make you a chicken!" Tyler Durden "He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson The noob forum Crack Plates Fastfreds media cookbook 60 agar/lc recipes Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide Trusted teks and linksSEED "PETRI" TEK Vent ur PC
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LtLurker
Lost Sailor



Registered: 01/03/18
Posts: 7,535
Loc: Borderlands
Last seen: 23 days, 14 hours
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Quote:
Smellyhobbit said:
Quote:
rumfor69 said: I have 2 flowhoods and I dont do LC cause I don't get perfection as is so I figured why compound that with something I cant visually see if it's clean to use. Like you said doing weekly cycles eliminates it's need. I make up batches of master jars A2G then store them in a mini fridge at 45°f until I need them for G2G.
How many expansions do you do from G2G? Do you set aside one of your receivers as a new master every time; and if so how many times are you comfortable repeating that?
Not everyone does, but i run my g2g in a continuing chain, choosing a master from each expansion's set of jars. My record is 10 expansions before contams overcome it from a mistake, typical get atleast 4 expansions from this method. This is in a SAB so if you have a FH you can probably do better than i have with it.
Edited by LtLurker (09/11/22 01:43 PM)
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ChildOfTheMoon
Stranger


Registered: 01/07/22
Posts: 143
Last seen: 11 months, 17 days
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Re: Shoebox, to progress? [Re: LtLurker] 2
#27945543 - 09/11/22 03:12 PM (1 year, 5 months ago) |
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I’m in the Netherlands on holiday and decided to ask a smart shop if they sold spores for stone producers (apparently that’s not a thing here, just cube grow kits with random other names ), and the guy behind the counter legit tried to explain me that “truffles have seeds”
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cozmyc
gentle modern ape



Registered: 06/20/21
Posts: 2,136
Last seen: 23 hours, 24 minutes
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Quote:
ChildOfTheMoon said: I’m in the Netherlands on holiday and decided to ask a smart shop if they sold spores for stone producers (apparently that’s not a thing here, just cube grow kits with random other names ), and the guy behind the counter legit tried to explain me that “truffles have seeds” 
Wait what? Maybe try multiple places. I'm sure there are tourist traps.
-------------------- You're conscious population 2 stardust ---------------------- and that's valuable
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