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Rusty2096
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Re: Second Flush pinning [Re: wazmo]
#27968178 - 09/25/22 09:13 PM (1 year, 4 months ago) |
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If you can replicate the results in a stable manner, and get similar result using 2 tubs (one with and one without the electrodes) - of course all this under control conditions with a good iso/pheno, you may need to patent some tubs soon
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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fahtster
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Re: Second Flush pinning [Re: Rusty2096] 5
#27968206 - 09/25/22 09:42 PM (1 year, 4 months ago) |
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There’s just no way to know if that myc would have grown those fruits anyway or if the electricity maybe even hindered a better flush
Just like there’s not way to tell if a lightning strike made more mushrooms grow in that area or if there was going to be mushrooms there anyway or even more than that would’ve grown without the lightning.
You usually won’t get fruits in an area that fruited already the previous flush. So, to me, it makes sense that the other side isn’t flushing because it already flushed heavy on that side. I want to see the experiment produce results that improve fruiting but it all seems like confirmation bias at this point and I’m not sure how to get past that problem because you can’t repeat the same grow twice and there’s a decent amount of variation of growth even within an isolated culture
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wazmo
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Third flush results [Re: fahtster] 1
#27973534 - 09/29/22 08:05 AM (1 year, 3 months ago) |
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After moving the electric grid to the previously non-electrified side, the third flush fruited more on the (newly) electric than on the non-electric side.
Electric side: wet 77.3g, dry 6g Non-electric side: wet 31.9g, dry 2.6g
So the electric side resulted in 131% higher yield than the non-electric side.
As @Fahtster and @cooleko pointed out, I need to use separate containers in order to have a good control condition.
So the upcoming experiment will use 12x 1-quart containers for 2 myco-quarts of colonized WBS + 4 quarts of coir, with half of the containers being electrified and the other half not.
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Rusty2096
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Re: Third flush results [Re: wazmo]
#27973542 - 09/29/22 08:08 AM (1 year, 3 months ago) |
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-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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DERRAYLD
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Re: Third flush results [Re: Rusty2096] 1
#27973665 - 09/29/22 09:34 AM (1 year, 3 months ago) |
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Confirmation bias unfortunately, you're just not understanding what we're saying.
Be very careful of taking the results so literally because there are so many variables at play here.
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Rusty2096
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Re: Third flush results [Re: DERRAYLD]
#27973672 - 09/29/22 09:37 AM (1 year, 3 months ago) |
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He literally just said he was trying again with 12 shoeboxes, 6 with and 6 without electrodes
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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DERRAYLD
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Re: Third flush results [Re: Rusty2096]
#27973681 - 09/29/22 09:40 AM (1 year, 3 months ago) |
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Good for him, I'm not arguing or trying to diminish his effort
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Rusty2096
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Re: Third flush results [Re: wazmo]
#27973714 - 09/29/22 09:57 AM (1 year, 3 months ago) |
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-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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cozmyc
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Re: Third flush results [Re: Rusty2096]
#27974358 - 09/29/22 06:04 PM (1 year, 3 months ago) |
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cool, Did you snap pic of latest flush?
-------------------- You're conscious population 2 stardust ---------------------- and that's valuable
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wazmo
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Re: Third flush results [Re: cozmyc] 1
#27976343 - 10/01/22 08:50 AM (1 year, 3 months ago) |
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Quote:
cozmyc said: cool, Did you snap pic of latest flush?
Yes, though this may have been after removing one early fruit from the electric side.
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wazmo
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Second experiment preparation [Re: wazmo] 1
#27978093 - 10/02/22 02:08 PM (1 year, 3 months ago) |
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I'd finished assembling the second experiment electrode grids: six acrylic plates, each with a 4x4 grid of stainless-steel jewelry pins connected together by a piece of 12AWG solid copper wire threaded through their eyes. These were then connected together by pieces of 18AWG high-voltage wire.
After the assembly and soldering, I then sealed the copper wires down the acrylic by applying silicone sealant to the underside.
When I looked at the results this morning, I remembered that I should have thought for a second before applying the silicone: I'd used acetoxy -cure silicone, which produces acetic acid upon curing. This had, of course, corroded the copper wire, making the already poor connection between the copper and the stainless steel even worse.
So I threw that assembly away, and am preparing to make a new one in which I use neutral cure silicone (GE RTV 2) instead.
Meanwhile I have three heavily-colonized jars of grain spawn ready to go.
Is there any problem with waiting a day or two to introduce these to the coir?
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Rusty2096
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Re: Second experiment preparation [Re: wazmo]
#27978110 - 10/02/22 02:20 PM (1 year, 3 months ago) |
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No problem waiting.
Your jars are bacterial
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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wazmo
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Re: Second experiment preparation [Re: Rusty2096] 1
#27978129 - 10/02/22 02:32 PM (1 year, 3 months ago) |
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Quote:
Rusty2096 said: No problem waiting.
Your jars are bacterial 
I was wondering about that. Is it just the condensation at the top that makes you say that? Is my grow doomed? I was hoping to have results from this experiment by Nov. 1 because I wanted to show them to some folks at a conference.
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fahtster
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Re: Second experiment preparation [Re: wazmo]
#27978135 - 10/02/22 02:35 PM (1 year, 3 months ago) |
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Condensation and long tendrils of ropey myc are a good indication. Not necessarily doomed but if they are bacterial, that’s a definite factor that’s going to skew your results.
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wazmo
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Re: Second experiment preparation [Re: fahtster] 1
#27978197 - 10/02/22 03:27 PM (1 year, 3 months ago) |
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Quote:
fahtster said: Condensation and long tendrils of ropey myc are a good indication. Not necessarily doomed but if they are bacterial, that’s a definite factor that’s going to skew your results.
Thanks. As long as I can get some fruiting, I can get comparable results that can show whether the electricity has any useful benefits (now that I have an adequate control with separate containers).
I'm going to have six 1-quart containers with electricity, six without, and a separate shoebox, all in the same SGFC to keep the humidity and temperature the same across all the containers.
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Rusty2096
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Re: Second experiment preparation [Re: wazmo]
#27978202 - 10/02/22 03:31 PM (1 year, 3 months ago) |
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Sadly, unclean spawn can reflect on the results. For example, you may have tubs having a harder time with the contaminants than others.
You would need clean spawn, ideally from an isolate for proper comparison.
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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rumfor69
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Re: Second experiment preparation [Re: Rusty2096]
#27978209 - 10/02/22 03:34 PM (1 year, 3 months ago) |
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Maybe the electricity will kill the contams!
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Rusty2096
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Re: Second experiment preparation [Re: rumfor69]
#27978211 - 10/02/22 03:35 PM (1 year, 3 months ago) |
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Quote:
rumfor69 said: Maybe the electricity will kill the contams!
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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wazmo
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Re: Second experiment preparation [Re: Rusty2096] 1
#27978232 - 10/02/22 03:49 PM (1 year, 3 months ago) |
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Quote:
Rusty2096 said: Sadly, unclean spawn can reflect on the results. For example, you may have tubs having a harder time with the contaminants than others.
You would need clean spawn, ideally from an isolate for proper comparison.
I'd intended to mix all the spawn and coir in a large bowl, trying for the most uniform mix possible. And I will weigh the mix that goes into each container and pack each to the same level.
I'd inoculated from agar wedges from plates that looked clean, but there is probably still a mix of genetics (I was in more of a hurry that I should have been and skipped an agar-to-agar transfer to isolate).
As for the bacterial problem, I'm not sure where the issue is. I've kept the WBS jars non-inoculated for a week or so and haven't seen any signs of bacteria in them, but that's probably not a good control. If I had more PC capacity (I only have a 9L PC) I could do more than 3 jars at once.
I've been using a SAB to do the transfers, and my agar plates haven't shown any signs of contamination (even the ones where I'd done agar-to-agar transfers).
Actually, I think I've figured it out... I'd assumed that the first red line meant 10psi and the second was 15psi, but according to this info I just found on my PC:

the second line was only 12.8psi max, so I've been under-sterilizing this whole time
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Rusty2096
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Re: Second experiment preparation [Re: wazmo]
#27978361 - 10/02/22 05:07 PM (1 year, 3 months ago) |
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That's a bummer. Good news is you figured it out at least.
-------------------- Currently looking for nothing. You guys who sent me stuff are straight up awesome!. We don't own things - things own us. Semi-solid liquid culture (SSLC)
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